Characterization of the Electrogenicity of Soybean (Glycine max L.) Roots : ATP Dependence and Effect of ATPase Inhibitors.

Abstract:

:The ATP-dependence of the membrane potential of soybean (Glycine max L. cv Williams '79) roots was determined by correlating the decrease in ATP levels with the decrease in the membrane potential caused by carbonyl cyanide m-chlorophenylhydrazone (CCCP) or cyanide. The effects of the ATPase inhibitors, vanadate and fluoride, were also examined in an attempt to establish the role of a plasma membrane localized electrogenic proton pumping ATPase in generating the membrane potential.The membrane potential of intact seedling roots bathed in artificial pond water was -167 +/- 27 millivolts (n = 130); the ATP level was 161 +/- 27 nanomoles per gram fresh weight (n = 102) when measured using rapid acid homogenization of the intact roots. Depolarization to the diffusion potential (-100 millivolts) occurred at about 90 nanomoles ATP per gram fresh weight, 50 to 70% of the control ATP level.Cyanide-induced ATP loss and membrane potential depolarization occurred at similar rates, but CCCP-induced ATP loss was slower than the rate of depolarization. CCCP may initially depolarize the membrane potential by increasing the proton permeability of the plasma membrane and short-circuiting the putative electrogenic proton pump. Vanadate and NaF caused a relatively slight depolarization of the membrane potential. Both inhibited microsomal ATPase activity substantially at the concentrations causing the depolarization.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Lew RR,Spanswick RM

doi

10.1104/pp.75.1.1

subject

Has Abstract

pub_date

1984-05-01 00:00:00

pages

1-6

issue

1

eissn

0032-0889

issn

1532-2548

journal_volume

75

pub_type

杂志文章
  • The GH3 acyl adenylase family member PBS3 regulates salicylic acid-dependent defense responses in Arabidopsis.

    abstract::The pbs3-1 mutant, identified in a screen for Arabidopsis (Arabidopsis thaliana) mutants exhibiting enhanced susceptibility to the avirulent Pseudomonas syringae pathogen DC3000 (avrPphB), also exhibits enhanced susceptibility to virulent P. syringae strains, suggesting it may impact basal disease resistance. Because ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.097691

    authors: Nobuta K,Okrent RA,Stoutemyer M,Rodibaugh N,Kempema L,Wildermuth MC,Innes RW

    更新日期:2007-06-01 00:00:00

  • Involvement of Macromolecule Biosynthesis in Auxin and Fusicoccin Enhancement of beta-Glucan Synthase Activity in Pea.

    abstract::In pea stem segments whose cuticle has been made permeable by abrading it, actinomycin D (ActD) and 80S ribosomal protein synthesis inhibitors such as cycloheximide (CHI) inhibit enhancement by indole 3-acetic acid (IAA) of the activity of the cell wall biosynthetic enzyme, glucan synthase I (GS). This supersedes earl...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.2.523

    authors: Ray PM

    更新日期:1987-10-01 00:00:00

  • Fluorescence Properties of Guard Cell Chloroplasts: EVIDENCE FOR LINEAR ELECTRON TRANSPORT AND LIGHT-HARVESTING PIGMENTS OF PHOTOSYSTEMS I AND II.

    abstract::The presence of chloroplasts in guard cells from leaf epidermis, coleoptile, flowers, and albino portions of variegated leaves was established by incident fluorescence microscopy, thus confirming the notion that guard cell chloroplasts are remarkably conserved. Room temperature emission spectra from a few chloroplasts...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.67.1.17

    authors: Zeiger E,Armond P,Melis A

    更新日期:1981-01-01 00:00:00

  • Induction and Accumulation of Heat Shock-Specific Poly(A) RNAs and Proteins in Soybean Seedlings during Arsenite and Cadmium Treatments.

    abstract::Northern blot hybridization analyzes revealed that poly(A(+)) RNAs homologous to eight heat shock (HS)-specific cDNA clones were induced by arsenite (As) or Cd treatments. The mRNAs accumulated slower, and maximum accumulations were consistently lower than HS-induced levels. Prolonged treatment with low concentrations...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.4.1048

    authors: Edelman L,Czarnecka E,Key JL

    更新日期:1988-04-01 00:00:00

  • The histidine kinase Hik34 is involved in thermotolerance by regulating the expression of heat shock genes in synechocystis.

    abstract::Histidine kinases (Hiks) in Synechocystis sp. PCC 6803 are involved in the transduction of signals associated with various kinds of environmental stress. To examine the potential role in thermotolerance of Hiks, we used genome microarray analysis to screen a Hik knockout library for mutations that affected the express...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.059097

    authors: Suzuki I,Kanesaki Y,Hayashi H,Hall JJ,Simon WJ,Slabas AR,Murata N

    更新日期:2005-07-01 00:00:00

  • Mitochondrial Contribution to Photosynthetic Metabolism (A Study with Barley (Hordeum vulgare L.) Leaf Protoplasts at Different Light Intensities and CO2 Concentrations).

    abstract::An oligomycin concentration that specifically inhibits oxidative phosphorylation was added to isolated barley (Hordeum vulgare L.) leaf protoplasts at various irradiances and carbon dioxide concentrations. At saturating as well as low light intensities, photosynthetic oxygen evolution was decreased as a result of the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.3.947

    authors: Kromer S,Malmberg G,Gardestrom P

    更新日期:1993-07-01 00:00:00

  • Combining experimental and predicted datasets for determination of the subcellular location of proteins in Arabidopsis.

    abstract::Substantial experimental datasets defining the subcellular location of Arabidopsis (Arabidopsis thaliana) proteins have been reported in the literature in the form of organelle proteomes built from mass spectrometry data (approximately 2,500 proteins). Subcellular location for specific proteins has also been published...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.065532

    authors: Heazlewood JL,Tonti-Filippini J,Verboom RE,Millar AH

    更新日期:2005-10-01 00:00:00

  • Constitutive expression of a miR319 gene alters plant development and enhances salt and drought tolerance in transgenic creeping bentgrass.

    abstract::MicroRNA319 (miR319) is one of the first characterized and conserved microRNA families in plants and has been demonstrated to target TCP (for TEOSINTE BRANCHED/CYCLOIDEA/PROLIFERATING CELL FACTORS [PCF]) genes encoding plant-specific transcription factors. MiR319 expression is regulated by environmental stimuli, sugge...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.208702

    authors: Zhou M,Li D,Li Z,Hu Q,Yang C,Zhu L,Luo H

    更新日期:2013-03-01 00:00:00

  • Mercury-induced Ethylene Formation and Abscission in Citrus and Coleus Explants.

    abstract::Mercury vapor induces ethylene formation and abscission in Citrus and Coleus explants. Both responses are markedly greater in the absence of CO(2). The stimulation of these metabolically complex processes indicates that the action of mercury vapor is not consistent with the more popular conception of mercury toxicity....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.4.628

    authors: Goren R,Siegel SM

    更新日期:1976-04-01 00:00:00

  • The multiple roles of conserved arginine 286 of 1-aminocyclopropane-1-carboxylate synthase. Coenzyme binding, substrate binding, and beyond.

    abstract::A pyridoxal 5'-phosphate (PLP)-dependent enzyme, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (S-adenosyl-L-Met methylthioadenosine-lyase, EC 4.4.1.14), catalyzes the conversion of S-adenosyl-L-methionine (AdoMet) to ACC. A tomato ACC synthase isozyme (LE-ACS2) with a deletion of 46 amino acids at the C termin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.121.3.913

    authors: Zhou H,Wang HW,Zhu K,Sui SF,Xu P,Yang SF,Li N

    更新日期:1999-11-01 00:00:00

  • l-Aspartate Transport into Pea Chloroplasts : Kinetic and Inhibitor Evidence for Multiple Transport Systems.

    abstract::The kinetics of l-aspartate transport into pea chloroplasts was studied in the presence and absence of transport inhibitors to determine whether multiple aspartate carriers exist. Transport was measured by the silicone oil centrifugation technique. Reciprocal plots of concentration-dependent transport rates were bipha...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.2.221

    authors: Werner-Washburne M,Keegstra K

    更新日期:1985-06-01 00:00:00

  • Rapid Hormone-induced Hyperpolarization of the Oat Coleoptile Transmembrane Potential.

    abstract::The effects of the plant growth substances indoleacetic acid (IAA) and fusicoccin on the transmembrane potential of Avena coleoptile cells (at 27-29 C) were studied. Fusicoccin caused hyperpolarization of the membrane potential which started after a lag of less than 20 seconds, and which on average reached -49 mv at a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.3.395

    authors: Cleland RE,Prins HB,Harper JR,Higinbotham N

    更新日期:1977-03-01 00:00:00

  • Variation in amounts of pyruvate, orthophosphate dikinase, and some other enzymes of the c(4) pathway in some wheat species.

    abstract::First leaves and flag leaves of the wheat species Triticum aestivum cv Anza (6x), T. boeoticum Boiss (2x) L. were examined for content of pyruvate, orthophosphate dikinase (PPDK), phosphoenolpyruvate carboxylase (PEPC), and ribulose 1,5-bisphosphate carboxylase (RuBPC) by protein blot analyses using antibodies to maiz...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.1.96

    authors: Aoyagi K,Bassham JA

    更新日期:1986-09-01 00:00:00

  • CO2 Uptake and Electron Transport Rates in Wild-Type and a Starchless Mutant of Nicotiana sylvestris (The Role and Regulation of Starch Synthesis at Saturating CO2 Concentrations).

    abstract::CO2 uptake rate, chlorophyll fluorescence, and 830-nm absorbance were measured in wild-type (wt) Nicotiana sylvestris (Speg. et Comes) and starchless mutant NS 458 leaves at different light intensities and CO2 concentrations. Initial slopes of the relationships between CO2 uptake and light and CO2 were similar, but th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.2.679

    authors: Eichelmann H,Laisk A

    更新日期:1994-10-01 00:00:00

  • A class I ADP-ribosylation factor GTPase-activating protein is critical for maintaining directional root hair growth in Arabidopsis.

    abstract::Membrane trafficking and cytoskeletal dynamics are important cellular processes that drive tip growth in root hairs. These processes interact with a multitude of signaling pathways that allow for the efficient transfer of information to specify the direction in which tip growth occurs. Here, we show that AGD1, a class...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.119529

    authors: Yoo CM,Wen J,Motes CM,Sparks JA,Blancaflor EB

    更新日期:2008-08-01 00:00:00

  • Expression of Escherichia coli glycogen synthase in the tubers of transgenic potatoes (Solanum tuberosum) results in a highly branched starch.

    abstract::A chimeric gene containing the patatin promoter and the transit-peptide region of the small-subunit carboxylase gene was utilized to direct expression of Escherichia coli glycogen synthase (glgA) to potato (Solanum tuberosum) tuber amyloplasts. Expression of the glgA gene product in tuber amyloplasts was between 0.007...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.4.1159

    authors: Shewmaker CK,Boyer CD,Wiesenborn DP,Thompson DB,Boersig MR,Oakes JV,Stalker DM

    更新日期:1994-04-01 00:00:00

  • Variations in leaf soluble amino acids and ammonium content in subtropical seagrasses related to salinity stress.

    abstract::A survey of leaf soluble amino acids was conducted for four subtropical seagrasses grown at several salinities. Proline functioned as an organic osmoticum in Halodule wrightii Aschers., Thallasia testudinum Banks ex Koenig, and Ruppia maritima L., while alanine functioned in an osmoregulatory capacity in Halophila eng...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.1.283

    authors: Pulich WM

    更新日期:1986-01-01 00:00:00

  • Regulation of key enzymes of sucrose biosynthesis in soybean leaves : effect of dark and light conditions and role of gibberellins and abscisic Acid.

    abstract::An important part in the understanding of the regulation of carbon partitioning within the leaf is to investigate the endogenous variations of parameters related to carbon metabolism. This study of diurnal changes in the activities of sucrose-synthesizing enzymes and levels of nonstructural carbohydrates in intact lea...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.3.1230

    authors: Cheikh N,Brenner ML

    更新日期:1992-11-01 00:00:00

  • Overexpression of ARGOS Genes Modifies Plant Sensitivity to Ethylene, Leading to Improved Drought Tolerance in Both Arabidopsis and Maize.

    abstract::Lack of sufficient water is a major limiting factor to crop production worldwide, and the development of drought-tolerant germplasm is needed to improve crop productivity. The phytohormone ethylene modulates plant growth and development as well as plant response to abiotic stress. Recent research has shown that modify...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00780

    authors: Shi J,Habben JE,Archibald RL,Drummond BJ,Chamberlin MA,Williams RW,Lafitte HR,Weers BP

    更新日期:2015-09-01 00:00:00

  • Grain Development Mutants of Barley ([alpha]-Amylase Production during Grain Maturation and Its Relation to Endogenous Gibberellic Acid Content).

    abstract::Barley (Hordeum vulgare L. Himalaya) mutants with altered grain morphology were isolated to investigate whether defects in grain development, possibly involving gibberellins (GAs) and abscisic acid, would lead to altered patterns of [alpha]-amylase gene expression. Following treatment with sodium azide, 75 mutants, ty...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.1.203

    authors: Green LS,Faergestad EM,Poole A,Chandler PM

    更新日期:1997-05-01 00:00:00

  • Adenosine kinase modulates root gravitropism and cap morphogenesis in Arabidopsis.

    abstract::Adenosine kinase (ADK) is a key enzyme that regulates intra- and extracellular levels of adenosine, thereby modulating methyltransferase reactions, production of polyamines and secondary compounds, and cell signaling in animals. Unfortunately, little is known about ADK's contribution to the regulation of plant growth ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.084798

    authors: Young LS,Harrison BR,Narayana Murthy UM,Moffatt BA,Gilroy S,Masson PH

    更新日期:2006-10-01 00:00:00

  • The Chickpea Early Flowering 1 (Efl1) Locus Is an Ortholog of Arabidopsis ELF3.

    abstract::In climates that experience short growing seasons due to drought, heat, or end-of-season frost, early flowering is a highly desirable trait for chickpea (Cicer arietinum). In this study, we mapped, sequenced, and characterized Early flowering3 (Efl3), an ortholog of Arabidopsis (Arabidopsis thaliana) EARLY FLOWERING3 ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00082

    authors: Ridge S,Deokar A,Lee R,Daba K,Macknight RC,Weller JL,Tar'an B

    更新日期:2017-10-01 00:00:00

  • Effects of short-term n(2) deficiency on N metabolism in legume nodules.

    abstract::The study aimed to test the hypothesis that ammonia production by Rhizobium bacteroids provides not only a source of nitrogen for growth but has a central regulatory role in maintaining the metabolic activity and functional integrity of the legume nodule. Production of ammonia in intact, attached nodules was interrupt...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.76.3.705

    authors: Atkins CA,Pate JS,Shelp BJ

    更新日期:1984-11-01 00:00:00

  • Abscisic Acid promotes both volume flow and ion release to the xylem in sunflower roots.

    abstract::The effect of abscisic acid (ABA) on the exudation process in excised sunflower root was investigated. A promotion of both ion flux and volume exudation rate was observed. Cutting off the external supply of ions to the roots markedly increased the magnitude of the ABA effect. The promotive effect of ABA on exudation r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.3.537

    authors: Glinka Z

    更新日期:1980-03-01 00:00:00

  • Lipid Characterization of an Enriched Plasma Membrane Fraction of Dunaliella salina Grown in Media of Varying Salinity.

    abstract::We have developed a rapid procedure for isolating a fraction enriched in plasma membrane from Dunaliella salina using an aqueous two-phase system (dextran/polyethylene glycol, 6.7%/6.7%). An enriched plasma membrane fraction, free of chloroplast and mitochondrial contamination, could be obtained in 2.5 hours. Plasma m...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.3.970

    authors: Peeler TC,Stephenson MB,Einspahr KJ,Thompson GA

    更新日期:1989-03-01 00:00:00

  • Isolation and characterization of soluble boron complexes in higher plants. The mechanism of phloem mobility of boron.

    abstract::Boron (B) polyol complexes have been isolated and characterized from the phloem sap of celery (Apium graveolens L.) and the extrafloral nectar of peach (Prunus persica L.). In celery the direct analysis of untreated phloem sap by matrix-assisted laser desorption-Fourier transform mass spectrometry, with verification b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.2.649

    authors: Hu H,Penn SG,Lebrilla CB,Brown PH

    更新日期:1997-02-01 00:00:00

  • Optimization of Light-Harvesting Pigment Improves Photosynthetic Efficiency.

    abstract::Maximizing light capture by light-harvesting pigment optimization represents an attractive but challenging strategy to improve photosynthetic efficiency. Here, we report that loss of a previously uncharacterized gene, HIGH PHOTOSYNTHETIC EFFICIENCY1 (HPE1), optimizes light-harvesting pigments, leading to improved phot...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00698

    authors: Jin H,Li M,Duan S,Fu M,Dong X,Liu B,Feng D,Wang J,Wang HB

    更新日期:2016-11-01 00:00:00

  • Inhibition of leaf process by p-fluorophenylalanine during induction of flowering in the cocklebur.

    abstract::The amino acid antimetabolite, DL-p-fluorophenylalanine (FPA), inhibited induction of flowering in the short-day cocklebur plant, Xanthium pensylvanicum Wall., primarily by interfering with processes occurring during the inductive dark period. At the concentrations used the inhibitor had little effect on subsequent ve...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.7.1185

    authors: Miller J,Ross C

    更新日期:1966-09-01 00:00:00

  • CO(2) Metabolism in Corn Roots. I. Kinetics of Carboxylation and Decarboxylation.

    abstract::The kinetics of (14)CO(2) carboxylation and decarboxylation in corn root tips were determined to ascertain the sequence of product formation and subsequent utilization, and to obtain further evidence to predict the enzymes mediating the carboxylation and decarboxylations. The carboxylation data indicated that the firs...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.5.712

    authors: Ting IP,Dugger WM

    更新日期:1967-05-01 00:00:00

  • Selectivity of alkali cation influx across the plasma membrane of oat roots: cation specificity of the plasma membrane ATPase.

    abstract::Influx of alkali cations (Li(+), Na(+), K(+), Rb(+), Cs(+)) across plasma membranes of cells of excised roots of Avena sativa cv. Goodfield was selective, but different, in the absence and in the presence of 1 mm CaSO(4). Ca(2+) reduced the influx rates of all of the alkali cations-especially Na(+) and Li(+). Transpor...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.4.641

    authors: Sze H,Hodges TK

    更新日期:1977-04-01 00:00:00