Expression of Escherichia coli glycogen synthase in the tubers of transgenic potatoes (Solanum tuberosum) results in a highly branched starch.

Abstract:

:A chimeric gene containing the patatin promoter and the transit-peptide region of the small-subunit carboxylase gene was utilized to direct expression of Escherichia coli glycogen synthase (glgA) to potato (Solanum tuberosum) tuber amyloplasts. Expression of the glgA gene product in tuber amyloplasts was between 0.007 and 0.028% of total protein in independent potato lines as determined by immunoblot analysis. Tubers from four transgenic potato lines were found to have a lowered specific gravity, a 30 to 50% reduction in the percentage of starch, and a decreased amylose/amylopectin ratio. Total soluble sugar content in these selected lines was increased by approximately 80%. Analysis of the starch from these potato lines also indicated a reduced phosphorous content. A very high degree of branching of the amylopectin fraction was detected by comparison of high and low molecular weight carbohydrate chains after debranching with isoamylase and corresponding high-performance liquid chromatography analysis of the products. Brabender viscoamylograph analysis and differential scanning calorimetry of the starches obtained from these transgenic potato lines also indicate a composition and structure much different from typical potato starch. Brabender analysis yielded very low stable paste viscosity values (about 30% of control values), whereas differential scanning calorimetry values indicated reduced enthalpy and gelatinization properties. The above parameters indicate a novel potato starch based on expression of the glgA E. coli gene product in transgenic potato.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Shewmaker CK,Boyer CD,Wiesenborn DP,Thompson DB,Boersig MR,Oakes JV,Stalker DM

doi

10.1104/pp.104.4.1159

subject

Has Abstract

pub_date

1994-04-01 00:00:00

pages

1159-66

issue

4

eissn

0032-0889

issn

1532-2548

pii

104/4/1159

journal_volume

104

pub_type

杂志文章
  • Phloem Transport of Amino Acids in Relation to their Cytosolic Levels in Barley Leaves.

    abstract::A comparison of barley (Hordeum vulgare L.) leaves was made between the cytosolic content of amino acids and sucrose as determined by subcellular fractionation and the corresponding concentration in phloem sap, which was collected continuously for up to 6 days from severed aphid stylets. Because amino acids were found...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.3.996

    authors: Winter H,Lohaus G,Heldt HW

    更新日期:1992-07-01 00:00:00

  • Solubilization of the starch-granule-bound starch synthase of normal maize kernels.

    abstract::The starch-granule-bound starch synthase from Zea mays has been solubilized with a recovery of between 50 and 84%. Chromatography of the solubilized enzyme on DEAE-Sepharose resolves two fractions of activity which may be distinguished by their response to citrate. Neither solubilized isoenzyme displays any significan...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.1.175

    authors: Macdonald FD,Preiss J

    更新日期:1983-09-01 00:00:00

  • Protein cryoprotective activity of a cytosolic small heat shock protein that accumulates constitutively in chestnut stems and is up-regulated by low and high temperatures.

    abstract::Heat shock, and other stresses that cause protein misfolding and aggregation, trigger the accumulation of heat shock proteins (HSPs) in virtually all organisms. Among the HSPs of higher plants, those belonging to the small HSP (sHSP) family remain the least characterized in functional terms. We analyzed the occurrence...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.035857

    authors: Lopez-Matas MA,Nuñez P,Soto A,Allona I,Casado R,Collada C,Guevara MA,Aragoncillo C,Gomez L

    更新日期:2004-04-01 00:00:00

  • Ca transport in membrane vesicles from pinto bean leaves and its alteration after ozone exposure.

    abstract::The influence of ozone on Ca(2+) transport in plant membranes from pinto bean (Phaseolus vulgaris L. var Pinto) leaves was investigated in vitro by means of a filtration method using purified vesicles. Two transport mechanisms located at the plasma membrane are involved in a response to ozone: (a) passive Ca(2+) influ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.2.788

    authors: Castillo FJ,Heath RL

    更新日期:1990-10-01 00:00:00

  • Compromised virus-induced gene silencing in RDR6-deficient plants.

    abstract::RNA silencing in plants serves as a potent antiviral defense mechanism through the action of small interfering RNAs (siRNAs), which direct RNA degradation. siRNAs can be derived directly from the viral genome or via the action of host-encoded RNA-dependent RNA polymerases (RDRs). Plant genomes encode multiple RDRs, an...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.132688

    authors: Vaistij FE,Jones L

    更新日期:2009-03-01 00:00:00

  • Bound auxin formation in growing stems.

    abstract::The term "bound auxin" is herein used to describe auxin conjugates insoluble in organic solvents which dissolve indoleacetic acid (IAA) and its derivatives, but hydrolyzable by NaOH to release IAA. Bound auxin from pea stems was fractionated into water-soluble, water-insoluble/NaOH-hydrolyzable, and insoluble componen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.2.197

    authors: Davies PJ

    更新日期:1976-02-01 00:00:00

  • Membrane glycerolipid remodeling triggered by nitrogen and phosphorus starvation in Phaeodactylum tricornutum.

    abstract::Diatoms constitute a major phylum of phytoplankton biodiversity in ocean water and freshwater ecosystems. They are known to respond to some chemical variations of the environment by the accumulation of triacylglycerol, but the relative changes occurring in membrane glycerolipids have not yet been studied. Our goal was...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.252395

    authors: Abida H,Dolch LJ,Meï C,Villanova V,Conte M,Block MA,Finazzi G,Bastien O,Tirichine L,Bowler C,Rébeillé F,Petroutsos D,Jouhet J,Maréchal E

    更新日期:2015-01-01 00:00:00

  • Antisense suppression of 2-cysteine peroxiredoxin in Arabidopsis specifically enhances the activities and expression of enzymes associated with ascorbate metabolism but not glutathione metabolism.

    abstract::The aim of this study was to characterize the effect of decreased 2-cysteine peroxiredoxin (2-CP) on the leaf anti-oxidative system in Arabidopsis. At three stages of leaf development, two lines of transgenic Arabidopsis mutants with decreased contents of chloroplast 2-CP were compared with wild type and a control lin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.124.2.823

    authors: Baier M,Noctor G,Foyer CH,Dietz KJ

    更新日期:2000-10-01 00:00:00

  • Developmental and Tissue-Specific Structural Alterations of the Cell-Wall Polysaccharides of Arabidopsis thaliana Roots.

    abstract::The plant cell wall is a dynamic structure that plays important roles in growth and development and in the interactions of plants with their environment and other organisms. We have used monoclonal antibodies that recognize different carbohydrate epitopes present in plant cell-wall polysaccharides to locate these epit...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.4.1413

    authors: Freshour G,Clay RP,Fuller MS,Albersheim P,Darvill AG,Hahn MG

    更新日期:1996-04-01 00:00:00

  • The Effect of Light on the Tricarboxylic Acid Cycle in Green Leaves: II. Intermediary Metabolism and the Location of Control Points.

    abstract::Long term feeding of acetate-2-(14)C, (14)CO(2), citrate-1,5-(14)C, fumarate-2,3-(14)C, and succinate-2,3-(14)C to mung bean (Phaseolus aureus L. var. Mungo) leaves in the dark gave labeling predominantly in tricarboxylic acid cycle intermediates. Kinetics of the intermediates during dark/light/dark transitions showed...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.53.6.886

    authors: Chapman EA,Graham D

    更新日期:1974-06-01 00:00:00

  • Selection of Nicotiana tabacum Haploids of High Photosynthetic Efficiency.

    abstract::Anthers of Nicotiana tabacum cultivar Wisconsin 38 were treated with the mutagen ethyl methane sulfonate and cultured to obtain populations of haploid plants of high genetic variability. The selection of these populations by their photosynthetic efficiency was carried out in a hydroponic culture chamber with a CO(2) a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.2.505

    authors: Medrano H,Primo-Millo E

    更新日期:1985-10-01 00:00:00

  • HEAT SHOCK FACTOR A8a Modulates Flavonoid Synthesis and Drought Tolerance.

    abstract::Drought is an important environmental factor affecting the growth and production of agricultural crops and fruits worldwide, including apple (Malus domestica). Heat shock factors (HSFs) have well-documented functions in stress responses, but their roles in flavonoid synthesis and the flavonoid-mediated drought respons...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.01106

    authors: Wang N,Liu W,Yu L,Guo Z,Chen Z,Jiang S,Xu H,Fang H,Wang Y,Zhang Z,Chen X

    更新日期:2020-11-01 00:00:00

  • Second positive phototropism results from coordinated co-action of the phototropins and cryptochromes.

    abstract::Phototropism and hypocotyl growth inhibition are modulated by the coaction of different blue-light photoreceptors and their signaling pathways. How seedlings integrate the activities of the different blue-light photoreceptors to coordinate these hypocotyl growth responses is still unclear. We have used time-lapse imag...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.018481

    authors: Whippo CW,Hangarter RP

    更新日期:2003-07-01 00:00:00

  • Deficiency of a plastidial adenylate kinase in Arabidopsis results in elevated photosynthetic amino acid biosynthesis and enhanced growth.

    abstract::An Arabidopsis (Arabidopsis thaliana) L. Heynh mutant deficient in an isoform of adenylate kinase (ADK; At2g37250) was isolated by reverse genetics. It contains a T-DNA insertion 377 bp downstream of the start point of transcription. The mutant lacks At2g37250 transcripts and has a mild reduction in total cellular ADK...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.056143

    authors: Carrari F,Coll-Garcia D,Schauer N,Lytovchenko A,Palacios-Rojas N,Balbo I,Rosso M,Fernie AR

    更新日期:2005-01-01 00:00:00

  • Enzymic Mechanism of Starch Breakdown in Germinating Rice Seeds: 8. Immunohistochemical Localization of beta-Amylase.

    abstract::Rabbit antiserum against beta-amylase isolated from germinating seeds of rice was produced, and its specific cross-reactivity with beta-amylase was confirmed by means of Ouchterlony double immunodiffusion and immunoelectrophoresis procedures. The cellular localization of beta-amylase was studied by indirect fluorescen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.64.2.337

    authors: Okamoto K,Akazawa T

    更新日期:1979-08-01 00:00:00

  • Regulation of Photosynthetic Activity in the Primary Leaves of Bean (Phaseolus vulgaris L.) by Materials Moving in the Water-conducting System.

    abstract::Endogenous factors which determine the photosynthetic capacity of the leaf were studied in the fully expanded, primary leaves of young seedings of bean (cv. Bulgarian). Following removal of the shoot above the primary leaf node and excision of all axillary buds, the primary leaves increased in area and thickness, in c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.64.2.285

    authors: Carmi A,Koller D

    更新日期:1979-08-01 00:00:00

  • Development of water conducting capacity in the root systems of young plants of corn and some other c4 grasses.

    abstract::Development of the primary and early nodal roots was studied in Zea mays L., Zea mexicana (Schrad.) Reeves & Mangelsd., Sorghum bicolor (L.) Moench., and Sorghum sudanese (Piper) Stapf. in relation to shoot development. In all the types studied all roots reached lengths of about 30 centimeters before the late metaxyle...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.4.1094

    authors: Wenzel CL,McCully ME,Canny MJ

    更新日期:1989-04-01 00:00:00

  • Effect of volatile methyl jasmonate on the oxylipin pathway in tobacco, cucumber, and arabidopsis.

    abstract::The effect of atmospheric methyl jasmonate on the oxylipin pathway was investigated in leaves of tobacco (Nicotiana tabacum L.), cucumber (Cucumis sativa L.), and Arabidopsis thaliana (L.). Differential sensitivities of test plants to methyl jasmonate were observed. Thus, different concentrations of methyl jasmonate w...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.4.1227

    authors: Avdiushko S,Croft KP,Brown GC,Jackson DM,Hamilton-Kemp TR,Hildebrand D

    更新日期:1995-12-01 00:00:00

  • Genetic and Physiological Analysis of a New Locus in Arabidopsis That Confers Resistance to 1-Aminocyclopropane-1-Carboxylic Acid and Ethylene and Specifically Affects the Ethylene Signal Transduction Pathway.

    abstract::A population of M2 seedlings of Arabidopsis thaliana was screened for mutants that were insensitive to the ethylene precursor 1-aminocyclopropane-1-carboxylate (ACC). Several independent lines were obtained and proved insensitive to both ACC and ethylene. Two lines were identified as alleles of a single recessive muta...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.2.401

    authors: Van Der Straeten D,Djudzman A,Van Caeneghem W,Smalle J,Van Montagu M

    更新日期:1993-06-01 00:00:00

  • Nitrate Assimilation during Vegetative Regrowth of Alfalfa.

    abstract::Dry matter accumulation, nitrate reductase activity of various organs, nitrate accumulation, nitrogen derived from nitrate, and nitrogen content were studied during 17 days of vegetative regrowth of harvested (detopped) alfalfa (Medicago sativa L.). Seedlings were grown in the glasshouse and treated with 0, 40, and 80...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.5.1052

    authors: Vance CP,Heichel GH

    更新日期:1981-11-01 00:00:00

  • Synthesis and uptake of cytoplasmically synthesized pyruvate, pi dikinase polypeptide by chloroplasts.

    abstract::Polyadenylated RNA was isolated from maize leaves and translated in vitro. In agreement with a previous report by others, we found among the translation products a 110-kilodalton pyruvate orthophosphate dikinase (PPDK) precursor that is about 16 kilodaltons larger than the polypeptide isolated from cells. This maize P...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.4.807

    authors: Aoyagi K,Bassham JA

    更新日期:1985-08-01 00:00:00

  • Synthesis and apparent turnover of Acid invertase in relation to invertase inhibitor in wounded sweet potato root tissue.

    abstract::Previously we showed that acid invertase activity increased and then decreased rapidly in wounded sweet potato (Ipomoea batatas Liam.) root tissue, and that the tissue contained a heat-stable, proteinaceous inhibitor with a molecular weight of about 19,500 daltons.In response to wounding of sweet potato root tissue, i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.5.879

    authors: Matsushita K,Uritani I

    更新日期:1977-05-01 00:00:00

  • Strigolactone Hormones and Their Stereoisomers Signal through Two Related Receptor Proteins to Induce Different Physiological Responses in Arabidopsis.

    abstract::Two α/β-fold hydrolases, KARRIKIN INSENSITIVE2 (KAI2) and Arabidopsis thaliana DWARF14 (AtD14), are necessary for responses to karrikins (KARs) and strigolactones (SLs) in Arabidopsis (Arabidopsis thaliana). Although KAI2 mediates responses to KARs and some SL analogs, AtD14 mediates SL but not KAR responses. To furth...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.240036

    authors: Scaffidi A,Waters MT,Sun YK,Skelton BW,Dixon KW,Ghisalberti EL,Flematti GR,Smith SM

    更新日期:2014-07-01 00:00:00

  • In vitro reconstitution of electron transport from glucose-6-phosphate and NADPH to nitrite

    abstract::An NADPH-dependent NO2--reducing system was reconstituted in vitro using ferredoxin (Fd) NADP+ oxidoreductase (FNR), Fd, and nitrite reductase (NiR) from the green alga Chlamydomonas reinhardtii. NO2- reduction was dependent on all protein components and was operated under either aerobic or anaerobic conditions. NO2- ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.1.303

    authors: Jin T,Huppe HC,Turpin DH

    更新日期:1998-05-01 00:00:00

  • Reduction of light-induced anthocyanin accumulation in inoculated sorghum mesocotyls. Implications for a compensatory role in the defense response.

    abstract::Sorghum (Sorghum bicolor L. Moench) accumulates the anthocyanin cyanidin 3-dimalonyl glucoside in etiolated mesocotyls in response to light. Inoculation with the nonpathogenic fungus Cochliobolus heterostrophus drastically reduced the light-induced accumulation of anthocyanin by repressing the transcription of the ant...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.3.979

    authors: Lo SC,Nicholson RL

    更新日期:1998-03-01 00:00:00

  • Stomatal Opening Is Induced in Epidermal Peels of Commelina communis L. by GTP Analogs or Pertussis Toxin.

    abstract::Pretreatment with pertussis toxin or microinjection of guanosine- 5[prime]-(3-thiotriphosphate) (GTP-[gamma]-S) into guard cells in peeled epidermis of Commelina communis L. promoted stomatal opening under subsaturating white light. Guanosine-5[prime]-(2-thiodiphosphate) (GDP-[beta]-S) and adenosine-5[prime]-(3-thiotr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.1.95

    authors: Lee HJ,Tucker EB,Crain RC,Lee Y

    更新日期:1993-05-01 00:00:00

  • Complementation of sugary-1 phenotype in rice endosperm with the wheat isoamylase1 gene supports a direct role for isoamylase1 in amylopectin biosynthesis.

    abstract::To examine the role of isoamylase1 (ISA1) in amylopectin biosynthesis in plants, a genomic DNA fragment from Aegilops tauschii was introduced into the ISA1-deficient rice (Oryza sativa) sugary-1 mutant line EM914, in which endosperm starch is completely replaced by phytoglycogen. A. tauschii is the D genome donor of w...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.051359

    authors: Kubo A,Rahman S,Utsumi Y,Li Z,Mukai Y,Yamamoto M,Ugaki M,Harada K,Satoh H,Konik-Rose C,Morell M,Nakamura Y

    更新日期:2005-01-01 00:00:00

  • Calcium Requirement for Indoleacetic Acid-induced Acidification by Avena Coleoptiles.

    abstract::The ionic specificity of IAA-induced acidification by Avena coleoptiles was studied, using zwitterionic, presumably impermeant buffers. The acidification was almost totally dependent on divalent cations with an order of effectiveness of Ca(2+) >/= Sr(2+) > Mn(2+), Mg(2+); whereas other polyvalent cations tested were i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.3.347

    authors: Cohen JD,Nadler KD

    更新日期:1976-03-01 00:00:00

  • Calcium Levels Affect the Ability to Immunolocalize Calmodulin to Cortical Microtubules.

    abstract::Calcium affects the stability of cortical microtubules (MTs) in lysed protoplasts. This calmodulin (CaM)-mediated interaction may provide a mechanism that serves to integrate cellular behavior with MT function. To test the hypothesis that CaM associates with these MTs, monoclonal antibodies were produced against CaM, ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.2.543

    authors: Fisher DD,Cyr RJ

    更新日期:1993-10-01 00:00:00

  • Growth of transplastomic cells expressing D-amino acid oxidase in chloroplasts is tolerant to D-alanine and inhibited by D-valine.

    abstract::Dual-conditional positive/negative selection markers are versatile genetic tools for manipulating genomes. Plastid genomes are relatively small and conserved DNA molecules that can be manipulated precisely by homologous recombination. High-yield expression of recombinant products and maternal inheritance of plastid-en...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.204107

    authors: Gisby MF,Mudd EA,Day A

    更新日期:2012-12-01 00:00:00