Abstract:
:A comparison of barley (Hordeum vulgare L.) leaves was made between the cytosolic content of amino acids and sucrose as determined by subcellular fractionation and the corresponding concentration in phloem sap, which was collected continuously for up to 6 days from severed aphid stylets. Because amino acids were found to be almost absent from the vacuoles, and because the amino acid patterns in the stroma and cytosol are similar, whole leaf contents could be taken as a measure of cytosolic amino acid levels for a comparison of data during a diurnal cycle. The results show that the pattern of amino acids in the phloem sap was very similar to the pattern in the cytosol. Therefore, we concluded that the overall process of transfer of amino acids from the cytosol of the source cells into the sieve tubes, although carrier mediated, may be a passive process and that the translocation of amino acids via the sieve tubes requires the mass flow of sucrose driven by the active sucrose transport involved by the phloem loading.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Winter H,Lohaus G,Heldt HWdoi
10.1104/pp.99.3.996subject
Has Abstractpub_date
1992-07-01 00:00:00pages
996-1004issue
3eissn
0032-0889issn
1532-2548journal_volume
99pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Under normal field growth conditions, canola (Brassica napus) seeds produce chloroplasts during early seed development and then catabolize the photosynthetic machinery during seed maturation, producing mature seeds at harvest that are essentially free of chlorophyll (Chl). However, frost exposure early in canola seed ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.084483
更新日期:2006-09-01 00:00:00
abstract::A MADS-box gene, MdMADS2, was isolated from the apple (Malus x domestica Borkh.) var Fuji and its developmental expression pattern was studied during flower development. MdMADS2 shares a high degree of amino acid sequence identity with the SQUAMOSA subfamily of genes. RNA blot analysis showed that MdMADS2 is transcrib...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.120.4.969
更新日期:1999-08-01 00:00:00
abstract::Blue light induces the differentiation of Chlamydomonas reinhardtii pregametes to gametes. The light-induced conversion of pregametes to gametes is protein synthesis dependent and proceeds only after a lag phase. Upon incubation in the dark, gametes lost their mating ability, resulting in dark-inactivated gametes. Rei...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.115.3.1241
更新日期:1997-11-01 00:00:00
abstract::A 108-fold purification of the sucrose synthetase from the extract of the shoot of bamboo Lelaba oldhami was achieved by ammonium sulfate fractionation, calcium phosphate gel adsorption, and chromatographic separations on Sephadex G-100 and diethylaminoethyl-cellulose columns. Some properties of this enzyme, namely th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.1.17
更新日期:1977-07-01 00:00:00
abstract::The Medicago truncatula line 2HA has a 500-fold greater capacity to regenerate plants in culture by somatic embryogenesis than wild-type Jemalong. We have compared proteomes of tissue cultures from leaf explants of these two lines. Both 2HA and Jemalong explants were grown on media containing the auxin 1-naphthaleneac...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.055277
更新日期:2005-04-01 00:00:00
abstract::Plant architecture is optimized for the local light environment. In response to foliar shade or neighbor proximity (low red to far-red light), some plant species exhibit shade-avoiding phenotypes, including increased stem and hypocotyl growth, which increases the likelihood of outgrowing competitor plants. If shade pe...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.241844
更新日期:2014-07-01 00:00:00
abstract::The accumulation of starch within photosynthetic tissues and within dedicated storage organs has been characterized extensively in many species, and a function in buffering carbon availability or in fueling later growth phases, respectively, has been proposed. However, developmentally regulated starch turnover within ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00916
更新日期:2016-12-01 00:00:00
abstract::How genes change their expression patterns over time is still poorly understood. Here, by conducting expression, functional, bioinformatic, and evolutionary analyses, we demonstrate that the differences between the Arabidopsis (Arabidopsis thaliana) APETALA1 (AP1) and CAULIFLOWER (CAL) duplicate genes in the time, spa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00320
更新日期:2016-06-01 00:00:00
abstract::We have introduced an ice nucleation gene (inaZ) from Pseudomonas syringae pv. syringae into Nicotiana tabacum, a freezing-sensitive species, and Solanum commersonii, a freezing-tolerant species. Transformants of both species showed increased ice nucleation activity over untransformed controls. The concentration of ic...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.4.1730
更新日期:1992-12-01 00:00:00
abstract::Changes in cytoplasmic Ca2+ concentration ([Ca2+]i) have been proposed to be involved in signal transduction pathways in response to a number of stimuli, including gravity and touch. The current hypothesis proposes that the development of gravitropic bending is correlated with a redistribution of [Ca2+]i in gravistimu...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.3.789
更新日期:1997-07-01 00:00:00
abstract::Plants have significantly more transcription factor (TF) families than animals and fungi, and plant TF families tend to contain more genes; these expansions are linked to adaptation to environmental stressors. Many TF family members bind to similar or identical sequence motifs, such as G-boxes (CACGTG), so it is diffi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.01086
更新日期:2017-10-01 00:00:00
abstract::Ethylene receptors are multispanning membrane proteins that negatively regulate ethylene responses via the formation of a signaling complex with downstream elements. To better understand their biochemical functions, we investigated the membrane topology and subcellular localization of CmERS1, a melon (Cucumis melo) et...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.080523
更新日期:2006-06-01 00:00:00
abstract::Photoinhibition-induced degradation of the D1 protein of the photosystem II reaction center was studied in intact pumpkin (Cucurbita pepo L.) leaves. Photoinhibition was observed to cause the cleavage of the D1 protein at two distinct sites. The main cleavage generated an 18-kD N-terminal and a 20-kD C-terminal degrad...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.4.1183
更新日期:1996-08-01 00:00:00
abstract::The interaction of tropisms is important in determining the final growth form of the plant body. In roots, gravitropism is the predominant tropistic response, but phototropism also plays a role in the oriented growth of roots in flowering plants. In blue or white light, roots exhibit negative phototropism that is medi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.013847
更新日期:2003-03-01 00:00:00
abstract::Two major proteins (the 7S and 11S globulins) of soybean (Glycine max) were simultaneously isolated by a simple method based on their different solubilities in dilute tris (hydroxymethyl) aminomethane buffers. The purified 7S globulins, which represented essentially the entire 7S soybean protein fraction capable of di...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.56.1.19
更新日期:1975-07-01 00:00:00
abstract::As part of a more detailed study on plant tumorigenesis, the action of gibberellic acid (GA(3)) in wounded potato tuber tissues as a model system has been evaluated. GA(3) stimulates total RNA synthesis in wounded tissues, the optimal concentration being 0.1 micromolar. The responsiveness of the tissue toward the horm...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.5.863
更新日期:1979-11-01 00:00:00
abstract::Wood is an important raw material and environmentally cost-effective renewable source of energy. However, the molecular biology of wood formation (i.e. secondary growth) is surprisingly understudied. A novel experimental system was employed to study the molecular regulation of secondary xylem formation in Arabidopsis....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.038844
更新日期:2004-06-01 00:00:00
abstract::Visible absorption spectra are presented for the Azolla caroliniana Willd.-Anabaena azollae Strass. association and the individual partners. Although absorption by the phycobiliproteins of the endophytic cyanobacterium clearly complements the absorption by the fern pigments, their contribution to the absorption spectr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.6.1479
更新日期:1981-12-01 00:00:00
abstract::Pattern-recognition receptors (PRRs), which consist of receptor kinases (RKs) and receptor-like proteins, sense microbe- and host-derived molecular patterns associated with pathogen infection to trigger immune responses in plants. Several kinases of the 46-member Arabidopsis (Arabidopsis thaliana) receptor-like cytopl...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00486
更新日期:2018-08-01 00:00:00
abstract::Previously the Arabidopsis (Arabidopsis thaliana) zinc finger protein OXIDATIVE STRESS2 (AtOXS2) and four OXS2-like (AtO2L) family members were described to play a role in stress tolerance and stress escape. For stress escape, SOC1 was a target of AtOXS2. However, for stress tolerance, the downstream targets were not ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00220
更新日期:2016-07-01 00:00:00
abstract::ZRP4, a 1.4-kb mRNA that preferentially accumulates in roots of young Zea mays L. plants, was identified by isolation of the corresponding cDNA clone. Genomic Southern analysis indicates that the zrp4 gene is represented once in the corn genome. The deduced ZRP4 polypeptide of 39,558 D is rich in leucine, serine, and ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.3.1001
更新日期:1993-07-01 00:00:00
abstract::The rate of (14)C-leucine and (3)H-uracil incorporation by tobacco cells (Nicotiana tabaccum var. Samsun N.N.) in suspension culture was simultaneously decreased by the addition of kinetin at concentrations above 2.5 x 10(-5)m. Ribosomal RNA was the first RNA species affected by kinetin. The purine derivatives, adenin...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.47.3.400
更新日期:1971-03-01 00:00:00
abstract::The rate of malic acid consumption in the Crassulacean acid metabolism (CAM) plant Kalanchoë daigremontiana Hamet et Perrier was found to be more rapid than the rate of photosynthetic oxygen evolution under all levels of irradiation by white light. This accounts for the accumulation of carbon dioxide in CAM tissues in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.3.568
更新日期:1982-03-01 00:00:00
abstract::To identify genetic loci involved in the regulation of organ-specific enzyme activities, a specific histochemical staining protocol was used in combination with quantitative trait locus (QTL) analysis. Using phosphoglucomutase (PGM) as an example, it is shown that enzyme activity can specifically, and with high resolu...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.027615
更新日期:2004-01-01 00:00:00
abstract::Low temperature is a seasonal abiotic stress that restricts native plant ranges and crop distributions. Two types of low-temperature stress can be distinguished: chilling and freezing. Much work has been done on the mechanisms by which chilling is sensed, but relatively little is known about how plants sense freezing....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00286
更新日期:2016-07-01 00:00:00
abstract::Poplar (Populus tremula x alba) trees (clone INRA 717-1-B4) were cultivated for 1 month in phytotronic chambers with two different levels of ozone (60 and 120 nL L(-1)). Foliar activities of shikimate dehydrogenase (EC 1.1.1.25), phenylalanine ammonia lyase (EC 4.3.1.5), and cinnamyl alcohol dehydrogenase (CAD, EC 1.1...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.031765
更新日期:2004-02-01 00:00:00
abstract::With the aid of an extracellular vibrating electrode, natural electric fields were detected and measured in the medium near growing roots and root hairs of barley seedlings. An exploration of these fields indicates that both the root as a whole, as well as individual root hairs, drive large steady currents through the...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.3.512
更新日期:1979-09-01 00:00:00
abstract::Kinematic analysis has provided important insights into the biology of growth by revealing the distribution of expansion within growing organs. Modern methods of kinematic analysis have made use of new image-tracking algorithms and computer-assisted evaluation, but these methods have yet to be adapted for examination ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.103226
更新日期:2007-10-01 00:00:00
abstract::The photorespiratory Arabidopsis (Arabidopsis thaliana) mutant gld1 (now designated mtkas-1) is deficient in glycine decarboxylase (GDC) activity, but the exact nature of the genetic defect was not known. We have identified the mtkas-1 locus as gene At2g04540, which encodes beta-ketoacyl-[acyl carrier protein (ACP)] s...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.104000
更新日期:2007-09-01 00:00:00
abstract::The permeability of the Chlorella pyrenoidosa membrane was studied by following the efflux of (14)C-intracellular material from cells which had been allowed to incorporate (14)CO(2) photosynthetically. It was observed that the efflux increased upon treatment with low concentrations (3-30 muM) of 2, 3-dichloro-1, 4-nap...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.51.2.363
更新日期:1973-02-01 00:00:00