Purification and Characterization of Sucrose Synthetase from the Shoot of Bamboo Leleba oldhami.

Abstract:

:A 108-fold purification of the sucrose synthetase from the extract of the shoot of bamboo Lelaba oldhami was achieved by ammonium sulfate fractionation, calcium phosphate gel adsorption, and chromatographic separations on Sephadex G-100 and diethylaminoethyl-cellulose columns. Some properties of this enzyme, namely thermal and pH stabilities, stabilization by aqueous glycerol, pH optimum, substrate specificities, effects of metallic ions, effects of sulfhydryl reagents, molecular weight, sedimentation constants, isoelectric point, and substrate saturation kinetics had been investigated.The substrate saturation kinetics indicated that the enzyme could be an allosteric enzyme with the saccharide substrates (sucrose and fructose) serving as the homotropic allosteric effectors in regulating the biosynthesis and degradation of sucrose.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Su JC

doi

10.1104/pp.60.1.17

subject

Has Abstract

pub_date

1977-07-01 00:00:00

pages

17-21

issue

1

eissn

0032-0889

issn

1532-2548

journal_volume

60

pub_type

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