An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine.

Abstract:

:In contrast to angiosperms, pines and other gymnosperms form well-developed suspensors in somatic embryogenic cultures. This creates a useful system to study suspensor biology. In a study of gene expression during the early stages of conifer embryogenesis, we identified a transcript, PtNIP1;1, that is abundant in immature loblolly pine (Pinus taeda) zygotic and somatic embryos, but is undetectable in later-stage embryos, megagametophytes, and roots, stems, and needles from 1 year-old seedlings. Analysis of PtNIP1;1 transcript in embryo proper and suspensor tissues by reverse transcription-polymerase chain reaction suggests preferential expression in the suspensor. Based on comparisons of derived amino acid sequences, PtNIP1;1 belongs to the nodulin-like members of the major intrinsic protein superfamily branch of the aquaporin (major intrinsic protein) superfamily. Through heterologous expression in Xenopus laevis oocytes and the yeast (Saccharomyces cerevisiae) fps1(-) mutant, PtNIP1;1 has been shown to be an active aquaglyceroporin.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Ciavatta VT,Morillon R,Pullman GS,Chrispeels MJ,Cairney J

subject

Has Abstract

pub_date

2001-12-01 00:00:00

pages

1556-67

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

127

pub_type

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