Abstract:
:Short-term ion uptake into roots of Limnobium stoloniferum was followed extracellularly with ion selective macroelectrodes. Cytosolic or vacuolar pH, together with the electrical membrane potential, was recorded with microelectrodes both located in the same young root hair. At the onset of chloride, phosphate, and nitrate uptake the membrane potential transiently decreased by 50 to 100 millivolts. During Cl(-) and H(2)PO(4) (-) uptake cytosolic pH decreased by 0.2 to 0.3 pH units. Nitrate induced cytosolic alkalinization by 0.19 pH units, indicating rapid reduction. The extracellular medium alkalinized when anion uptake exceeded K(+) uptake. During fusicoccin-dependent plasmalemma hyperpolarization, extracellular and cytosolic pH remained rather constant. Upon K(+) absorption, FC intensified extracellular acidification and intracellular alkalinization (from 0.31 to 0.4 pH units). In the presence of Cl(-) FC induced intracellular acidification. Since H(+) fluxes per se do not change the pH, recorded pH changes only result from fluxes of the stronger ions. The extra- and intracellular pH changes, together with membrane depolarization, exclude mechanisms as K(+)/A(-) symport or HCO(3) (-)/A(-) antiport for anion uptake. Though not suitable to reveal the actual H(+)/A(-) stoichiometry, the results are consistent with an H(+)/A(-) cotransport mechanism.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Ullrich CI,Novacky AJdoi
10.1104/pp.94.4.1561subject
Has Abstractpub_date
1990-12-01 00:00:00pages
1561-7issue
4eissn
0032-0889issn
1532-2548journal_volume
94pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::A light-dependent tyrosine kinase activity is present in soluble extracts from the cyanobacterium Prochlorothrix hollandica. The substrate of this tyrosine kinase activity is a soluble 88-kD protein that is phosphorylated when cultures of P. hollandica are adapted to high-light conditions. This phosphoprotein was iden...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.2.629
更新日期:1994-06-01 00:00:00
abstract::Many plants flower in response to seasonal changes in daylength. This response often varies between accessions of a single species. We studied the variation in photoperiod response found in the model species Arabidopsis (Arabidopsis thaliana). Seventy-two accessions were grown under six daylengths varying in 2-h inter...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.140772
更新日期:2010-01-01 00:00:00
abstract::Using a novel screening procedure, we have selected a new class of mutant from the cyanobacterium Synechococcus PCC7942 that fails to adapt to growth at an extremely low inorganic carbon (Ci) concentration. The mutant (Tm17) reported in this study grows normally at or above air levels of CO2 (340 [mu]L L-1) but does n...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.2.605
更新日期:1994-02-01 00:00:00
abstract::Auxin-binding protein 1 subsp. mays (ABP1) has been suggested as a receptor mediating auxin-induced cell expansion and differentiation. In maize (Zea mays), ABP1 is encoded by a single gene, Abp1. The TATA and CAAT promoter elements as well as the transcriptional start site were previously identified and all were foun...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.124.1.369
更新日期:2000-09-01 00:00:00
abstract::Among the four uppermost leaves of greenhouse-grown plants of Xanthium strumarium L. the content of abscisic acid per unit fresh or dry weight was highest in the youngest leaf and decreased gradually with increasing age of the leaves. Expressed per leaf, the second youngest leaf was richest in ABA; the amount of ABA p...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.58.2.169
更新日期:1976-08-01 00:00:00
abstract::The pollen-pistil interactions that result in the stimulation of ethylene production and petal senescence in carnation (Dianthus caryophyllus L.) flowers were investigated. Pollination of White Sim flowers with Starlight pollen elicited an increase in ethylene production by styles, leading to increased petal ethylene ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.4.1405
更新日期:1995-08-01 00:00:00
abstract::Auxins and cytokinins support cell division in tissue and cell cultures. In cytokinin-independent pear (Pyrus communis) cells, omission of 2,4-dichlorophenoxyacetic acid (2,4-D) from the medium for two successive transfers leads to rapid cell lysis, unless the osmolarity is raised to 0.4 molar with mannitol. Use of th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.5.814
更新日期:1979-11-01 00:00:00
abstract::Glyoxylate at a concentration of 10 millimolar caused 50% inhibition of decarboxylation of 20 millimolar [1-(14)C]glycine and accompanying synthesis of serine in a mitochondria-enriched preparation from tobacco (Nicotiana tabacum var. John Williams Broadleaf) leaves. None of the other compounds tested including format...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.1.61
更新日期:1982-07-01 00:00:00
abstract::Arabidopsis (Arabidopsis thaliana) GARP (Golden2, ARR-B, Psr1) family transcription factors, GOLDEN2-LIKE1 and -2 (GLK1/2), function in different biological processes; however, whether and how these transcription factors modulate the response to abscisic acid (ABA) remain unknown. In this study, we used a glk1 glk2 do...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.01466
更新日期:2019-04-01 00:00:00
abstract::The relationship between gravitropism and surface electrical potentials was studied using etiolated epicotyls of adzuki bean (Phaseolus angularis). Early downward curvature (or transient positive gravitropic response) was observed about 1 min after gravistimulation. The downward curvature was closely related to the sp...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.3.875
更新日期:1994-07-01 00:00:00
abstract::A novel alliinase (EC 4.4.1.4) was detected and purified from the roots of the Amazonian medicinal plant Petiveria alliacea. The isolated enzyme is a heteropentameric glycoprotein composed of two alpha-subunits (68.1 kD each), one beta-subunit (56.0 kD), one gamma-subunit (24.8 kD), and one delta-subunit (13.9 kD). Th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.142430
更新日期:2009-11-01 00:00:00
abstract::The sugar compositions of root hairs of a variety of plant species were determined. Root hairs of legumes had very similar compositions, whereas those of different families varied widely. Only approximately 50% of the weight of the root hairs of legumes could be accounted for by sugar. Up to 80% of the weight of root ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.2.638
更新日期:1988-02-01 00:00:00
abstract::Three aspartate aminotransferase isoenzymes were identified from extracts of carrot (Daucus carota L.) cell suspension cultures. These isoenzymes were separated by DEAE chromatography and were analyzed on native gradient polyacrylamide gels. The relative molecular weights of the isoenzymes were 111,000 +/- 5000, 105,0...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.92.3.587
更新日期:1990-03-01 00:00:00
abstract::The enzyme aldose (aldehyde) reductase was partially purified (142-fold) and characterized from Euonymus japonica leaves. The reductase, a dimer, had an average molecular weight of 67,000 as determined by gel filtration on Sephadex G-100. The enzyme was NADPH specific and reduced a broad range of substrates including ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.4.972
更新日期:1986-04-01 00:00:00
abstract::Bound auxin metabolism in cultured crown-gall tumor cells and pith callus of tobacco was examined by feeding radiolabeled auxins and auxin conjugates. In all tissues fed [(14)C]indoleacetic acid (IAA), at least one-third of the IAA was decarboxylated, and most of the remaining radiolabel occurred in a compound(s) whic...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.2.315
更新日期:1986-02-01 00:00:00
abstract::The adaptability of root system architecture to unevenly distributed mineral nutrients in soil is a key determinant of plant performance. The molecular mechanisms underlying nitrate dependent plasticity of lateral root branching across the different root types of maize are only poorly understood. In this study, detail...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.01885
更新日期:2016-03-01 00:00:00
abstract::The first compound in the series of reactions leading to the ester conjugates of indole-3-acetic acid (IAA) in kernels of Zea mays sweet corn is the acyl alkyl acetal, 1-O-indol-3-ylacetyl-beta-D-glucose (1-O-IAGlu). The enzyme catalyzing the synthesis of this compound is UDP-glucose:indol-3-ylacetate glucosyl-transf...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.1.4
更新日期:1990-01-01 00:00:00
abstract::The light dependence of D1 phosphorylation is unique to higher plants, being constitutive in cyanobacteria and algae. In a photoautotrophic higher plant, Spirodela oligorrhiza, grown in greenhouse conditions under natural diurnal cycles of solar irradiation, the ratio of phosphorylated versus total D1 protein (D1-P in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.013441
更新日期:2002-12-01 00:00:00
abstract::The idea that embolized xylem vessels can be refilled while adjacent vessels remain under tension is difficult to accept if the cavitated vessels remain hydraulically connected to vessels under tension. A mechanism by which embolized conduits could be hydraulically isolated from adjacent conduits requires the existenc...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.123.3.1015
更新日期:2000-07-01 00:00:00
abstract::When pea (Pisum sativum) seeds were wetted, a sharp front separated the wet and dry portions, the seeds swelled, and the water content in the wetted portion continued to increase for a long time. A model was proposed and tested that takes into account these three characteristics and in particular does not postulate a ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.57.2.153
更新日期:1976-02-01 00:00:00
abstract::Axes of soybean seeds are tolerant to dehydration at 6 hours of imbibition, but susceptible to dehydration injury if dried at 36 hours of imbibition. Smooth microsomal membranes were isolated from axes imbibed for 6 hours (dehydration tolerant state) and 36 hours (dehydration susceptible state) before and after dehydr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.76.3.759
更新日期:1984-11-01 00:00:00
abstract::The partitioning of noncyclic photosynthetic electron transport between net fixation of CO(2) and collective O(2)-dependent, dissipative processes such as photorespiration has been examined in intact leaf tissue from Nicotiana tabacum. The method involves simultaneous application of CO(2) exchange and pulse modulated ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.4.1322
更新日期:1989-08-01 00:00:00
abstract::Histone H2B monoubiquitination (H2Bub) is being recognized as a regulatory mechanism that controls a range of cellular processes in plants, but the molecular mechanisms of H2Bub that are involved in responses to biotic stress are largely unknown. In this study, we used wild-type and H2Bub loss-of-function mutations of...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.234567
更新日期:2014-04-01 00:00:00
abstract::Vanadate (Na(3)VO(4)) selectively and reversibly affects the rhythmic movement of Albizzia julibrissin leaflets. Leaflets floated on 1 millimolar vanadate open at the same rate or more rapidly than controls, but closure is inhibited. After 6 to 24 hours incubation, the inhibition can be reversed by a 24-to 48-hour per...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.5.905
更新日期:1979-11-01 00:00:00
abstract::Durum wheat (Triticum turgidum L. subsp. durum Desf.) Line 149 contains two novel major genes for excluding Na(+) from leaf blades, named Nax1 and Nax2. The genes were separated into families containing a single gene and near-isogenic homozygous lines were selected. Lines containing either Nax1 or Nax2 had lower rates...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.086538
更新日期:2006-12-01 00:00:00
abstract::Previous work indicated more polysomes bound to pea (Pisum sativum cv Progress No. 9) thylakoids in light than in the dark, in vivo (LE Fish, AT Jagendorf 1982 Plant Physiol 69: 814-825). With isolated intact chloroplasts incubated in darkness, addition of MgATP had no effect but 24 to 74% more RNA was thylakoid-bound...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.84.1.31
更新日期:1987-05-01 00:00:00
abstract::The major nucleoside triphosphatase (NTPase) activities in mammalian and pea (Pisum sativum L.) nuclei are associated with enzymes that are very similar both biochemically and immunochemically. The major NTPase from rat liver nuclei appears to be a 46-kD enzyme that represents the N-terminal portion of lamins A and C,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.101.3.1005
更新日期:1993-03-01 00:00:00
abstract::ADP-glucose pyrophosphorylase catalyzes the synthesis of ADP-glucose (ADP-Glc) from Glc-1-phosphate (G-1-P) and ATP. Kinetic studies were performed to define the nature of the reaction, both in the presence and absence of allosteric effector molecules. When 3-phosphoglycerate (3-PGA), the putative physiological activa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.149450
更新日期:2010-02-01 00:00:00
abstract::A new indole-3-acetic acid metabolite was isolated from broad bean (Vicia faba L. cv Chukyo) seedlings. It was a conjugate of dioxindole-3-acetic acid, aspartic acid, and glucose and was identified as 3-(O-beta-glucosyl)-2-indolone-3-acetylaspartic acid (molecular weight 484) from ultraviolet, infrared, nuclear magnet...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.79.3.667
更新日期:1985-11-01 00:00:00
abstract::Microcomplement fixation was employed to compare the immunological differences that occur between purified inhibitor I from potato tubers, the four purified protomers that comprise it, and inhibitor I from tuber and leaf extracts. Total inhibitors of chymotrypsin and trypsin in leaves of seven genera of the Solanaceae...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.50.5.627
更新日期:1972-11-01 00:00:00