Isomerization of 1-O-indol-3-ylacetyl-beta-D-glucose. Enzymatic hydrolysis of 1-O, 4-O, and 6-O-indol-3-ylacetyl-beta-D-glucose and the enzymatic synthesis of indole-3-acetyl glycerol by a hormone metabolizing complex.

Abstract:

:The first compound in the series of reactions leading to the ester conjugates of indole-3-acetic acid (IAA) in kernels of Zea mays sweet corn is the acyl alkyl acetal, 1-O-indol-3-ylacetyl-beta-D-glucose (1-O-IAGlu). The enzyme catalyzing the synthesis of this compound is UDP-glucose:indol-3-ylacetate glucosyl-transferase (IAGlu synthase). The IAA moiety of the high energy compound 1-O-IAGlu may be enzymatically transferred to myo-inositol or to glycerol or the 1-O-IAGlu may be enzymatically hydrolyzed. Alternatively, nonenzymatic acyl migration may occur to yield the 2-O, 4-O, and 6-O esters of IAA and glucose. The 4-O and 6-O esters may then be enzymatically hydrolyzed to yield free IAA and glucose. This work reports new enzymatic activities, the transfer of IAA from 1-O-IAGlu to glycerol, and the enzyme-catalyzed hydrolysis of 4-O and 6-O-IAGlu. Data is also presented on the rate of non-enzymatic acyl migration of IAA from the 1-O to the 4-O and 6-O positions of glucose. We also report that enzymes catalyzing the synthesis of 1-O-IAGlu and the hydrolysis of 1-O, 4-O, and 6-O-IAGlu fractionate as a hormone metabolizing complex. The association of synthetic and hydrolytic capabilities in enzymes which cofractionate may have physiological significance.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Kowalczyk S,Bandurski RS

doi

10.1104/pp.94.1.4

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

4-12

eissn

0032-0889

issn

1532-2548

journal_volume

94

pub_type

杂志文章
  • TOPP4 Regulates the Stability of PHYTOCHROME INTERACTING FACTOR5 during Photomorphogenesis in Arabidopsis.

    abstract::In plants, photoreceptors transfer light signals to phytochrome-interacting factors (PIFs), inducing the rapid phosphorylation and degradation of PIFs to promote photomorphogenesis. However, the phosphatase responsible for PIF dephosphorylation remains unknown. In this study, we identified a type 1 protein phosphatase...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.01729

    authors: Yue J,Qin Q,Meng S,Jing H,Gou X,Li J,Hou S

    更新日期:2016-03-01 00:00:00

  • Comparative Effects of Ancymidol and Its Analogs on Growth of Peas and Ent-Kaurene Oxidation in Cell-Free Extracts of Immature Marah macrocarpus Endosperm.

    abstract::The plant growth retardant alpha-cyclopropyl-alpha-(4-methyoxyphenyl)-5-pyrimidine methyl alcohol (ancymidol) and a series of analogs of this substance in which one or more of the substituents were varied were tested for their comparative biological activity. The compounds were tested as inhibitors of internode elonga...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.3.707

    authors: Coolbaugh RC,Swanson DI,West CA

    更新日期:1982-03-01 00:00:00

  • Biosynthesis of Protein Amino Acids in Plant Tissue Culture IV Isotope Competition Experiments using Glucose-U-C and Potential Intermediates.

    abstract::The isotope competition method with glucose-U-(14)C as a carbon source has been used to determine whether or not selected compounds contribute carbon to the biosynthesis of protein amino acids in cells derived from Paul's Scarlet Rose. Of 48 compounds tested, 15 contributed carbon to protein amino acids. The results s...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.7.941

    authors: Dougall DK,Fulton MM

    更新日期:1967-07-01 00:00:00

  • Isolation of Guard Cell Protoplasts from Mechanically Prepared Epidermis of Vicia faba Leaves.

    abstract::A method for isolating guard cell protoplasts (GCP) from mechanically prepared epidermis of Vicia faba is described. Epidermis was prepared by homogenizing leaves in a Waring blender in a solution of 10% Ficoll, 5 millimolar CaCl(2), and 0.1% polyvinylpyrrolidone 40 (PVP). Attached mesophyll and epidermal cells were r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.4.1382

    authors: Kruse T,Tallman G,Zeiger E

    更新日期:1989-08-01 00:00:00

  • Molecular and genetic characterization of a non-climacteric phenotype in melon reveals two loci conferring altered ethylene response in fruit.

    abstract::Fruit ripening and abscission are associated with an ethylene burst in several melon (Cucumis melo) genotypes. In cantaloupe as in other climacteric fruit, exogenous ethylene can prematurely induce abscission, ethylene production, and ripening. Melon genotypes without fruit abscission or without ethylene burst also ex...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.010613

    authors: Périn C,Gomez-Jimenez M,Hagen L,Dogimont C,Pech JC,Latché A,Pitrat M,Lelièvre JM

    更新日期:2002-05-01 00:00:00

  • A nodule-specific dicarboxylate transporter from alder is a member of the peptide transporter family.

    abstract::Alder (Alnus glutinosa) and more than 200 angiosperms that encompass 24 genera are collectively called actinorhizal plants. These plants form a symbiotic relationship with the nitrogen-fixing actinomycete Frankia strain HFPArI3. The plants provide the bacteria with carbon sources in exchange for fixed nitrogen, but th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.032102

    authors: Jeong J,Suh S,Guan C,Tsay YF,Moran N,Oh CJ,An CS,Demchenko KN,Pawlowski K,Lee Y

    更新日期:2004-03-01 00:00:00

  • Inositol Metabolism in Plants. III. Conversion of Myo-inositol-2-H to Cell Wall Polysaccharides in Sycamore (Acer pseudoplatanus L.) Cell Culture.

    abstract::Prolonged growth of cell cultures of sycamore (Acer pseudoplatanus L.) on agar medium containing myo-inositol-2-(3)H resulted in incorporation of label predominately into uronosyl and pentosyl units of cell wall polysaccharides. Procedures normally used to distinguish between pectic substance and hemicellulose yielded...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.9.1489

    authors: Roberts RM,Loewus F

    更新日期:1966-11-01 00:00:00

  • Apoplasmic and Protoplasmic Water Transport through the Parenchyma of the Potato Storage Organ.

    abstract::Stationary volume fluxes through living and denatured parenchyma slices of the potato (Solanum tuberosum L.) storage organ were studied to estimate the hydraulic conductivity of the cell wall and to evaluate the significance of water transport through protoplasts, cell walls, and intercellular spaces. Slices were plac...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.115.3.1089

    authors: Michael W,Schultz A,Meshcheryakov AB,Ehwald R

    更新日期:1997-11-01 00:00:00

  • Preparation of Membrane Vesicles Enriched in ATP-Dependent Proton Transport from Suspension Cultures of Tomato Cells.

    abstract::Membranes enriched in ATP-dependent proton transport were prepared from suspension cultures of tomato cells (Lycopersicon esculentum Mill cv VF36). Suspension cultures were a source of large quantities of membranes from rapidly growing, undifferentiated cells. Proton transport activity was assayed as quench of acridin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.1.69

    authors: Dupont FM,Zabala Mde G

    更新日期:1985-01-01 00:00:00

  • Acclimation to Fluctuating Light Impacts the Rapidity of Response and Diurnal Rhythm of Stomatal Conductance.

    abstract::Plant acclimation to growth light environment has been studied extensively; however, the majority of these studies have focused on light intensity and photo-acclimation, with few studies exploring the impact of dynamic growth light on stomatal acclimation and behavior. To assess the impact of growth light regime on st...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.01809

    authors: Matthews JSA,Vialet-Chabrand S,Lawson T

    更新日期:2018-03-01 00:00:00

  • Trehalose as cryoprotectant for the freeze preservation of carrot and tobacco cells.

    abstract::Suspension cultures of carrot (Daucus carota, line C1), tobacco (Nicotiana tabacum, line TX1), and Nicotiana plumbaginifolia (line NP) were frozen under controlled conditions with trehalose as the sole cryoprotectant. Maximal post-thaw viability (71-74%), measured by phenosafranin dye exclusion, was obtained with the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.2.430

    authors: Bhandal IS,Hauptmann RM,Widholm JM

    更新日期:1985-06-01 00:00:00

  • Enhancement of wound-induced ethylene synthesis by ethylene in preclimacteric cantaloupe.

    abstract::Although intact fruits of unripe cantaloupe (Cucumis melo L.) produce very little ethylene, a massive increase in ethylene production occurred in response to excision. The evidence indicates that this wound ethylene is produced from methionine via 1-aminocyclopropanecarboxylic acid (ACC) as in ripening fruits. Excisio...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.2.317

    authors: Hoffman NE,Yang SF

    更新日期:1982-02-01 00:00:00

  • Biochemical characterization of a spearmint mutant that resembles peppermint in monoterpene content.

    abstract::A radiation-induced mutant of Scotch spearmint (Mentha x gracilis) was shown to produce an essential oil containing principally C3-oxygenated p-menthane monoterpenes that are typical of peppermint, instead of the C6-oxygenated monoterpene family characteristic of spearmint. In vitro measurement of all of the enzymes r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.3.744

    authors: Croteau R,Karp F,Wagschal KC,Satterwhite DM,Hyatt DC,Skotland CB

    更新日期:1991-07-01 00:00:00

  • GROWTH REGULATING FACTOR5 stimulates Arabidopsis chloroplast division, photosynthesis, and leaf longevity.

    abstract::Arabidopsis (Arabidopsis thaliana) leaf development relies on subsequent phases of cell proliferation and cell expansion. During the proliferation phase, chloroplasts need to divide extensively, and during the transition from cell proliferation to expansion, they differentiate into photosynthetically active chloroplas...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.256180

    authors: Vercruyssen L,Tognetti VB,Gonzalez N,Van Dingenen J,De Milde L,Bielach A,De Rycke R,Van Breusegem F,Inzé D

    更新日期:2015-03-01 00:00:00

  • Sugar Potentiation of Fatty Acid and Triacylglycerol Accumulation.

    abstract::Photosynthetically derived sugar provides carbon skeletons for lipid biosynthesis. We used mutants of Arabidopsis (Arabidopsis thaliana) and the expression of oleogenic factors to investigate relationships among sugar availability, lipid synthesis, and the accumulation of triacylglycerol (TAG) in leaf tissue. The adg1...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00828

    authors: Zhai Z,Liu H,Xu C,Shanklin J

    更新日期:2017-10-01 00:00:00

  • Ectopic expression of constitutively activated RACB in barley enhances susceptibility to powdery mildew and abiotic stress.

    abstract::Small RAC/ROP-family G proteins regulate development and stress responses in plants. Transient overexpression and RNA interference experiments suggested that the barley (Hordeum vulgare) RAC/ROP protein RACB is involved in susceptibility to the powdery mildew fungus Blumeria graminis f. sp. hordei. We created transgen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.066613

    authors: Schultheiss H,Hensel G,Imani J,Broeders S,Sonnewald U,Kogel KH,Kumlehn J,Hückelhoven R

    更新日期:2005-09-01 00:00:00

  • Modification of Herbicide Binding to Photosystem II in Two Biotypes of Senecio vulgaris L.

    abstract::THE PRESENT STUDY COMPARES THE BINDING AND INHIBITORY ACTIVITY OF TWO PHOTOSYSTEM II INHIBITORS: 3-(3,4-dichlorophenyl)-1,1-dimethylurea (diuron [DCMU]) and 2-chloro-4-(ethylamine)-6-(isopropyl amine)-S-triazene (atrazine). Chloroplasts isolated from naturally occurring triazine-susceptible and triazine-resistant biot...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.64.6.995

    authors: Pfister K,Radosevich SR,Arntzen CJ

    更新日期:1979-12-01 00:00:00

  • The circadian clock that controls gene expression in Arabidopsis is tissue specific.

    abstract::The expression of CHALCONE SYNTHASE (CHS) expression is an important control step in the biosynthesis of flavonoids, which are major photoprotectants in plants. CHS transcription is regulated by endogenous programs and in response to environmental signals. Luciferase reporter gene fusions showed that the CHS promoter ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.005405

    authors: Thain SC,Murtas G,Lynn JR,McGrath RB,Millar AJ

    更新日期:2002-09-01 00:00:00

  • A novel phospholipase D of Arabidopsis that is activated by oleic acid and associated with the plasma membrane.

    abstract::Oleate-dependent phospholipase D (PLD; EC 3.1.4.4) has been reported in animal systems, but its molecular nature is unkown. Multiple PLDs have been characterized in plants, but none of the previously cloned PLDs exhibits the oleate-activated activity. Here, we describe the biochemical and molecular identification and ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:

    authors: Wang C,Wang X

    更新日期:2001-11-01 00:00:00

  • Identification of microRNAs involved in pathogen-associated molecular pattern-triggered plant innate immunity.

    abstract::Pathogen-associated molecular patterns (PAMPs) trigger plant defenses when perceived by surface-localized immune receptors. PAMP-triggered immunity (PTI) plays a vital role in the resistance of plants to numerous potential pathogens. MicroRNA (miRNA) biogenesis is known to be important for PTI, but miRNA species invol...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.151803

    authors: Li Y,Zhang Q,Zhang J,Wu L,Qi Y,Zhou JM

    更新日期:2010-04-01 00:00:00

  • Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei.

    abstract::We describe a simple and highly effective means for global identification of genes that are expressed within specific cell types within complex tissues. It involves transgenic expression of nuclear-targeted green fluorescent protein in a cell-type-specific manner. The fluorescent nuclei are then purified from homogena...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.115246

    authors: Zhang C,Barthelson RA,Lambert GM,Galbraith DW

    更新日期:2008-05-01 00:00:00

  • Effect of Na(3)VO(4) on the P State of Nitella translucens.

    abstract::The capacity of sodium orthovanadate to inhibit the plasmalemma H(+) ATPase of Nitella translucens internodal cells in vivo was tested. Here we show that 1 millimolar vanadate added externally depolarizes strongly and permanently the membrane potential, both in dark and light, to the Nernst potential for potassium con...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.1.91

    authors: Cruz-Mireles RM,Ortega-Blake I

    更新日期:1991-05-01 00:00:00

  • Dual regulation role of GH3.5 in salicylic acid and auxin signaling during Arabidopsis-Pseudomonas syringae interaction.

    abstract::Salicylic acid (SA) plays a central role in plant disease resistance, and emerging evidence indicates that auxin, an essential plant hormone in regulating plant growth and development, is involved in plant disease susceptibility. GH3.5, a member of the GH3 family of early auxin-responsive genes in Arabidopsis (Arabido...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.106021

    authors: Zhang Z,Li Q,Li Z,Staswick PE,Wang M,Zhu Y,He Z

    更新日期:2007-10-01 00:00:00

  • The Suitability of a Quantitative Spectrophotometric Assay for Phenylalanine Ammonia-lyase Activity in Barley, Buckwheat, and Pea Seedlings.

    abstract::It has been suggested by others that the spectrophotometric assay of phenylalanine ammonia-lyase based on changes in absorbance at 290 nm may be complicated by a side reaction involving transamination from phenylalanine onto alpha-keto acids. This would lead to the production of phenylpyruvate which would spontaneousl...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.3.412

    authors: Saunders JA,McClure JW

    更新日期:1974-09-01 00:00:00

  • Subcellular localization and membrane topology of the melon ethylene receptor CmERS1.

    abstract::Ethylene receptors are multispanning membrane proteins that negatively regulate ethylene responses via the formation of a signaling complex with downstream elements. To better understand their biochemical functions, we investigated the membrane topology and subcellular localization of CmERS1, a melon (Cucumis melo) et...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.080523

    authors: Ma B,Cui ML,Sun HJ,Takada K,Mori H,Kamada H,Ezura H

    更新日期:2006-06-01 00:00:00

  • Adenylate gradients and Ar:O(2) effects on legume nodules: I. Mathematical models.

    abstract::Mathematical models were developed to test the likelihood that large cytosolic adenylate concentration gradients exist across the bacteria-infected cells of legume nodules. Previous studies hypothesized that this may be the case to account for the unusually low adenylate energy charge (AEC; 0.65) measured in the plant...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.032318

    authors: Wei H,Atkins CA,Layzell DB

    更新日期:2004-02-01 00:00:00

  • Optical coherence microscopy. A technology for rapid, in vivo, non-destructive visualization of plants and plant cells.

    abstract::We describe the development and utilization of a new imaging technology for plant biology, optical coherence microscopy (OCM), which allows true in vivo visualization of plants and plant cells. This novel technology allows the direct, in situ (e.g. plants in soil), three-dimensional visualization of cells and events i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.123.1.3

    authors: Hettinger JW,de la Peña Mattozzi M,Myers WR,Williams ME,Reeves A,Parsons RL,Haskell RC,Petersen DC,Wang R,Medford JI

    更新日期:2000-05-01 00:00:00

  • Manipulation of in vivo sorbitol production alters boron uptake and transport in tobacco

    abstract::Recent evidence that some species can retranslocate boron as complexes with sugar alcohols in the phloem suggests a possible mechanism for enhancing boron efficiency. We investigated the relationship between sugar alcohol (sorbitol) content, boron uptake and distribution, and translocation of foliar-applied, isotopica...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.119.2.735

    authors: Bellaloui N,Brown PH,Dandekar AM

    更新日期:1999-02-01 00:00:00

  • Biosynthesis of Indoleacetic Acid from Tryptophan-C in Cell-free Extracts of Pea Shoot Tips.

    abstract::A 2-step, 1-dimensional thin-layer chromatographic procedure for isolating indoleacetic acid (IAA) was developed and utilized in investigations of the biosynthesis of IAA from tryptophan-(14)C in cell-free extracts of pea (Pisum sativum L.) shoot tips. Identification of a (14)C-product as IAA was by (a) co-chromatogra...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.12.1787

    authors: Moore TC,Shaner CA

    更新日期:1967-12-01 00:00:00

  • Varying Photoperiod, Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase and CO(2) Uptake in Thalassiosira fluviatilis (Bacillariophyceae).

    abstract::The purpose of this research was to test the hypothesis that acclimation of the unicellular marine alga, Thalassiosira fluviatilis Hustedt, to short photoperiods results in decreased cellular concentrations of ribulose 1,5-bisphosphate carboxylase/oxygenase and decreased rates of light-saturated CO(2) uptake. Cells we...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.3.833

    authors: Hobson LA,Morris WJ,Guest KP

    更新日期:1985-11-01 00:00:00