Effect of Na(3)VO(4) on the P State of Nitella translucens.

Abstract:

:The capacity of sodium orthovanadate to inhibit the plasmalemma H(+) ATPase of Nitella translucens internodal cells in vivo was tested. Here we show that 1 millimolar vanadate added externally depolarizes strongly and permanently the membrane potential, both in dark and light, to the Nernst potential for potassium consistent with pump inhibition by vanadate. From the results it is clear that the H(+) ATPase is always active, under light or dark conditions, in contradiction with the widespread idea of pump inactivation by darkness. The changes in conductance for light, dark, and vanadate-induced conditions are analyzed. The effect of dark on membrane passive permeabilities and on the possibility that some plasmalemma channels could be regulated by a phosphorylation-dephosphorylation process is discussed.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Cruz-Mireles RM,Ortega-Blake I

doi

10.1104/pp.96.1.91

subject

Has Abstract

pub_date

1991-05-01 00:00:00

pages

91-7

issue

1

eissn

0032-0889

issn

1532-2548

journal_volume

96

pub_type

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