Abstract:
:The kinetics of (14)CO(2) carboxylation and decarboxylation in corn root tips were determined to ascertain the sequence of product formation and subsequent utilization, and to obtain further evidence to predict the enzymes mediating the carboxylation and decarboxylations. The carboxylation data indicated that the first product was oxaloacetate followed by malate and aspartate. Malate was the first stable product which could be detected. Decarboxylation data indicated that a large fraction of the (14)CO(2) release and turnover of (14)C was accountable for by a decrease in malate: however, essentially all labeled amino acids turned over rapidly and at a greater rate than organic acids. The data generally support the hypothesis that CO(2) fixation in corn root tips is via P-enolpyruvate carboxylase and malic dehydrogenase and that subsequent malate metabolism is for the most part by direct decarboxylation, possibly by the malic enzyme.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Ting IP,Dugger WMdoi
10.1104/pp.42.5.712subject
Has Abstractpub_date
1967-05-01 00:00:00pages
712-8issue
5eissn
0032-0889issn
1532-2548journal_volume
42pub_type
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