Effect of water stress on cell division and cell-division-cycle 2-like cell-cycle kinase activity in wheat leaves


:In wheat (Triticum aestivum) seedlings subjected to a mild water stress (water potential of -0.3 MPa), the leaf-elongation rate was reduced by one-half and the mitotic activity of mesophyll cells was reduced to 42% of well-watered controls within 1 d. There was also a reduction in the length of the zone of mesophyll cell division to within 4 mm from the base compared with 8 mm in control leaves. However, the period of division continued longer in the stressed than in the control leaves, and the final cell number in the stressed leaves reached 85% of controls. Cyclin-dependent protein kinase enzymes that are required in vivo for DNA replication and mitosis were recovered from the meristematic zone of leaves by affinity for p13(suc1). Water stress caused a reduction in H1 histone kinase activity to one-half of the control level, although amounts of the enzyme were unaffected. Reduced activity was correlated with an increased proportion of the 34-kD Cdc2-like kinase (an enzyme sharing with the Cdc2 protein of other eukaryotes the same size, antigenic sites, affinity for p13(suc1), and H1 histone kinase catalytic activity) deactivated by tyrosine phosphorylation. Deactivation to 50% occurred within 3 h of stress imposition in cells at the base of the meristematic zone and was therefore too fast to be explained by a reduction in the rate at which cells reached mitosis because of slowing of growth; rather, stress must have acted more immediately on the enzyme. The operation of controls slowing the exit from the G1 and G2 phases is discussed. We suggest that a water-stress signal acts on Cdc2 kinase by increasing phosphorylation of tyrosine, causing a shift to the inhibited form and slowing cell production.


Plant Physiol


Plant physiology


Schuppler U,He PH,John PC,Munns R




Has Abstract


1998-06-01 00:00:00












  • Identification of BFN1, a bifunctional nuclease induced during leaf and stem senescence in Arabidopsis.

    abstract::Nuclease I enzymes are responsible for the degradation of RNA and single-stranded DNA during several plant growth and developmental processes, including senescence. However, in the case of senescence the corresponding genes have not been reported. We describe the identification and characterization of BFN1 of Arabidop...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Pérez-Amador MA,Abler ML,De Rocher EJ,Thompson DM,van Hoof A,LeBrasseur ND,Lers A,Green PJ

    更新日期:2000-01-01 00:00:00

  • Suppression of a vegetative MADS box gene of potato activates axillary meristem development.

    abstract::Potato MADS box 1 (POTM1) is a member of the SQUAMOSA-like family of plant MADS box genes isolated from an early stage tuber cDNA library. The RNA of POTM1 is most abundant in vegetative meristems of potato (Solanum tuberosum), accumulating specifically in the tunica and corpus layers of the meristem, the procambium, ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Rosin FM,Hart JK,Van Onckelen H,Hannapel DJ

    更新日期:2003-04-01 00:00:00

  • Effects of Salinity on Water Transport of Excised Maize (Zea mays L.) Roots.

    abstract::The root pressure probe was used to determine the effects of salinity on the hydraulic properties of primary roots of maize (Zea mays L. cv Halamish). Maize seedlings were grown in nutrient solutions modified by additions of NaCl and/or extra CaCl(2) so that the seedlings received one of four treatments: Control, plus...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Azaizeh H,Steudle E

    更新日期:1991-11-01 00:00:00

  • Photographic survey of the occurrence of bundle-sheath extensions in deciduous dicots.

    abstract::In a survey of over 300 nonevergreen dicots in 69 families, many species were found to have translucent patterns attributed to the presence of bundle-sheath extensions (BSE) on the small and ultimate veinlets. The BSE have been shown by others to inhibit transverse air movement within leaves, and it has been suggested...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: McClendon JH

    更新日期:1992-08-01 00:00:00

  • Apparent absence of a redox requirement for blue light activation of pump current in broad bean guard cells.

    abstract::In guard cells, membrane hyperpolarization in response to a blue light (BL) stimulus is achieved by the activation of a plasma membrane H(+)-ATPase. Using the patch clamp technique on broad bean (Vicia faba) guard cells we demonstrate that both steady-state- and BL-induced pump currents require ATP and are blocked by ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Taylor AR,Assmann SM

    更新日期:2001-01-01 00:00:00

  • Dissection of the light signal transduction pathways regulating the two early light-induced protein genes in Arabidopsis.

    abstract::The expression of light-regulated genes in plants is controlled by different classes of photoreceptors that act through a variety of signaling molecules. During photomorphogenesis, the early light-induced protein (Elip) genes are among the first to be induced. To understand the light signal transduction pathways that ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Harari-Steinberg O,Ohad I,Chamovitz DA

    更新日期:2001-11-01 00:00:00

  • Abscisic Acid promotes both volume flow and ion release to the xylem in sunflower roots.

    abstract::The effect of abscisic acid (ABA) on the exudation process in excised sunflower root was investigated. A promotion of both ion flux and volume exudation rate was observed. Cutting off the external supply of ions to the roots markedly increased the magnitude of the ABA effect. The promotive effect of ABA on exudation r...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Glinka Z

    更新日期:1980-03-01 00:00:00

  • Short-term leaf elongation kinetics of maize in response to salinity are independent of the root.

    abstract::The essentiality of roots to the short-term responses of leaf elongation to salinity was tested by removing the roots of maize (Zea mays L.) from the shoots and comparing the initial short-term response of leaf elongation to that with intact plants. Eightday-old seedlings growing in solution culture were treated with ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Cramer GR,Bowman DC

    更新日期:1991-03-01 00:00:00

  • Purification and Characteristics of Sorbitol-6-phosphate Dehydrogenase from Loquat Leaves.

    abstract::To study the role of sorbitol-6-phosphate dehydrogenase in sorbitol synthesis in leaves of Rosaceous plants, properties of the enzyme and its presence in several plants in the family was investigated. The activity of the enzyme, which catalyzes an NADP-dependent oxidation of the substrate to glucose-6-phosphate, was d...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Hirai M

    更新日期:1981-02-01 00:00:00

  • Peroxygenase-Catalyzed Fatty Acid Epoxidation in Cereal Seeds (Sequential Oxidation of Linoleic Acid into 9(S),12(S),13(S)-Trihydroxy-10(E)-Octadecenoic Acid).

    abstract::Peroxygenase-catalyzed epoxidation of oleic acid in preparations of cereal seeds was investigated. The 105,000g particle fraction of oat (Avena sativa) seed homogenate showed high peroxygenase activity, i.e. 3034 [plus or minus] 288 and 2441 [plus or minus] 168 nmol (10 min)-1 mg-1 protein in two cultivars, whereas th...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Hamberg M,Hamberg G

    更新日期:1996-03-01 00:00:00

  • DRT111/SFPS Splicing Factor Controls Abscisic Acid Sensitivity during Seed Development and Germination.

    abstract::RNA splicing is a fundamental mechanism contributing to the definition of the cellular protein population in any given environmental condition. DNA-DAMAGE REPAIR/TOLERATION PROTEIN111 (DRT111)/SPLICING FACTOR FOR PHYTOCHROME SIGNALING is a splicing factor previously shown to interact with phytochrome B and characteriz...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Punzo P,Ruggiero A,Possenti M,Perrella G,Nurcato R,Costa A,Morelli G,Grillo S,Batelli G

    更新日期:2020-06-01 00:00:00

  • Epigenetic transcriptional silencing and 5-azacytidine-mediated reactivation of a complex transgene in rice.

    abstract::Despite a growing number of reports indicating non-Mendelian inheritance of transgene expression in monocots, no detailed description of the structure and stability of the transgene exists for transformants generated by direct DNA-transfer techniques, making the cause for these observations difficult to determine. In ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Kumpatla SP,Teng W,Buchholz WG,Hall TC

    更新日期:1997-10-01 00:00:00

  • Production of a Brassica napus Low-Molecular Mass Acyl-Coenzyme A-Binding Protein in Arabidopsis Alters the Acyl-Coenzyme A Pool and Acyl Composition of Oil in Seeds.

    abstract::Low-molecular mass (10 kD) cytosolic acyl-coenzyme A-binding protein (ACBP) has a substantial influence over fatty acid (FA) composition in oilseeds, possibly via an effect on the partitioning of acyl groups between elongation and desaturation pathways. Previously, we demonstrated that the expression of a Brassica nap...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Yurchenko O,Singer SD,Nykiforuk CL,Gidda S,Mullen RT,Moloney MM,Weselake RJ

    更新日期:2014-06-01 00:00:00

  • Cinnamyl Alcohol Dehydrogenase: Identification of New Sites of Promoter Activity in Transgenic Poplar.

    abstract::Stem sections from poplar that were stably transformed with a eucalypt cinnamyl alcohol dehydrogenase promoter-[beta]-glucuronidase construct were prepared by using either a technique routinely used in herbaceous species or a technique designed to take into account the particular anatomy of woody plants. Although both...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Hawkins S,Samaj J,Lauvergeat V,Boudet A,Grima-Pettenati J

    更新日期:1997-02-01 00:00:00

  • Malate Decarboxylation by Kalanchoë daigremontiana Mitochondria and Its Role in Crassulacean Acid Metabolism.

    abstract::Mitochondria isolated from Kalanchoë daigremontiana, a Crassulacean acid metabolism plant, decarboxylate added malate to pyruvate at rates of up to 100 micromoles per hour per milligram original chlorophyll in the presence of ADP. Omitting ADP reduces this rate by approximately 50%. Antimycin A inhibits malate decarbo...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Day DA

    更新日期:1980-04-01 00:00:00

  • Phosphate Treatment Strongly Inhibits New Arbuscule Development But Not the Maintenance of Arbuscule in Mycorrhizal Rice Roots.

    abstract::Phosphorus (P) is a crucial nutrient for plant growth, but its availability to roots is limited in soil. Arbuscular mycorrhizal (AM) symbiosis is a promising strategy for improving plant P acquisition. However, P fertilizer reduces fungal colonization (P inhibition) and compromises mycorrhizal P uptake, warranting stu...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Kobae Y,Ohmori Y,Saito C,Yano K,Ohtomo R,Fujiwara T

    更新日期:2016-05-01 00:00:00

  • Analysis of Photosynthetic Antenna Function in a Mutant of Arabidopsis thaliana (L.) Lacking trans-Hexadecenoic Acid.

    abstract::Several lines of evidence support the proposal that the unusual chloroplast-specific lipid acyl group Delta3,trans-hexadecenoic acid (trans-C(16:1)) stimulates the formation or maintenance of the oligomeric form of the light-harvesting chlorophyll a/b complex (LHCP). To assess the functional significance of this appar...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: McCourt P,Browse J,Watson J,Arntzen CJ,Somerville CR

    更新日期:1985-08-01 00:00:00

  • Diurnal starch accumulation and utilization in phosphorus-deficient soybean plants.

    abstract::The effects of phosphorus deficiency on carbohydrate accumulation and utilization in 34-day-old soybean (Glycine max L. Merr.) plants were characterized over a diurnal cycle to evaluate the mechanisms by which phosphorus deficiency restricts plant growth. Phosphorus deficiency decreased the net CO(2) exchange rate thr...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Qiu J,Israel DW

    更新日期:1992-01-01 00:00:00

  • Inhibition and stimulation of root respiration in pisum and plantago by hydroxamate : its consequences for the assessment of alternative path activity.

    abstract::The contribution of the alternative pathway in root respiration of Pisum sativum L. cv Rondo, Plantago lanceolata L., and Plantago major L. ssp major was determined by titration with salicylhydroxamate (SHAM) in the absence and presence of cyanide. SHAM completely inhibited the cyanide-resistant component of root resp...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: de Visser R,Blacquière T

    更新日期:1984-07-01 00:00:00

  • Pathway-level acceleration of glycogen catabolism by a response regulator in the cyanobacterium Synechocystis species PCC 6803.

    abstract::Response regulators of two-component systems play pivotal roles in the transcriptional regulation of responses to environmental signals in bacteria. Rre37, an OmpR-type response regulator, is induced by nitrogen depletion in the unicellular cyanobacterium Synechocystis species PCC 6803. Microarray and quantitative rea...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Osanai T,Oikawa A,Numata K,Kuwahara A,Iijima H,Doi Y,Saito K,Hirai MY

    更新日期:2014-04-01 00:00:00

  • Flavone synthases from Medicago truncatula are flavanone-2-hydroxylases and are important for nodulation.

    abstract::Flavones are important copigments found in the flowers of many higher plants and play a variety of roles in plant adaptation to stress. In Medicago species, flavones also act as signal molecules during symbiotic interaction with the diazotropic bacterium Sinorhizobium meliloti. They are the most potent nod gene induce...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Zhang J,Subramanian S,Zhang Y,Yu O

    更新日期:2007-06-01 00:00:00

  • Metabolism of tryptophan in petioles of coleus.

    abstract::Auxin precursors retard abscission when applied to debladed petioles of Coleus blumei Benth. The d and l forms of tryptophan are equally effective in retarding abscission. Tryptamine is more effective than is tryptophan. Both compounds apparently are converted to auxin through an aldehyde intermediate. The evidence pr...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Valdovinos JG,Perley JE

    更新日期:1966-12-01 00:00:00

  • Cis-acting elements essential for light regulation of the nuclear gene encoding the A subunit of chloroplast glyceraldehyde 3-phosphate dehydrogenase in Arabidopsis thaliana.

    abstract::We report the characterization of cis-acting elements involved in light regulation of the nuclear gene (GapA) that encodes the A subunit of glyceraldehyde 3-phosphate dehydrogenase in Arabidopsis thaliana. Our previous deletion analyses indicate that the -277 to -195 upstream region of GapA is essential for light indu...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Park SC,Kwon HB,Shih MC

    更新日期:1996-12-01 00:00:00

  • Distinct roles for mitogen-activated protein kinase signaling and CALMODULIN-BINDING TRANSCRIPTIONAL ACTIVATOR3 in regulating the peak time and amplitude of the plant general stress response.

    abstract::To survive environmental challenges, plants have evolved tightly regulated response networks, including a rapid and transient general stress response (GSR), followed by well-studied stress-specific responses. The mechanisms underpinning the GSR have remained elusive, but a functional cis-element, the rapid stress resp...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Bjornson M,Benn G,Song X,Comai L,Franz AK,Dandekar AM,Drakakaki G,Dehesh K

    更新日期:2014-10-01 00:00:00

  • The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis.

    abstract::In many plant species, a subset of the genes of the chloroplast genome is transcribed by RpoTp, a nuclear-encoded plastid-targeted RNA polymerase. Here, we describe the positional cloning of the SCABRA3 (SCA3) gene, which was found to encode RpoTp in Arabidopsis (Arabidopsis thaliana). We studied one weak (sca3-1) and...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Hricová A,Quesada V,Micol JL

    更新日期:2006-07-01 00:00:00

  • Peroxidase Release Induced by Ozone in Sedum album Leaves: Involvement of Ca.

    abstract::The effect of ozone was studied on the peroxidase activity from various compartments of Sedum album leaves (epidermis, intercellular fluid, residual cell material, and total cell material). The greatest increase following a 2-hour ozone exposure (0.4 microliters O(3) per liter) was observed in extracellular peroxidase...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Castillo FJ,Penel C,Greppin H

    更新日期:1984-04-01 00:00:00

  • Induction and Accumulation of Heat Shock-Specific Poly(A) RNAs and Proteins in Soybean Seedlings during Arsenite and Cadmium Treatments.

    abstract::Northern blot hybridization analyzes revealed that poly(A(+)) RNAs homologous to eight heat shock (HS)-specific cDNA clones were induced by arsenite (As) or Cd treatments. The mRNAs accumulated slower, and maximum accumulations were consistently lower than HS-induced levels. Prolonged treatment with low concentrations...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Edelman L,Czarnecka E,Key JL

    更新日期:1988-04-01 00:00:00

  • Salinity stress inhibits bean leaf expansion by reducing turgor, not wall extensibility.

    abstract::Treatment of bean (Phaseolus vulgaris L.) seedlings with low levels of salinity (50 or 100 millimolar NaCl) decreased the rate of light-induced leaf cell expansion in the primary leaves over a 3 day period. This decrease could be due to a reduction in one or both of the primary cellular growth parameters: wall exten...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Neumann PM,Van Volkenburgh E,Cleland RE

    更新日期:1988-01-01 00:00:00

  • A new 1-aminocyclopropane-1-carboxylic acid-conjugating activity in tomato fruit.

    abstract::A new conjugate, 1-(gamma-L-glutamylamino)cyclopropane-1-carboxylic acid (GACC), of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) is identified. The only previously identified conjugate of ACC is 1-(malonylamino)cyclopropane-1-carboxylic acid (MACC). GACC, not MACC, was the major conjugate formed ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Martin MN,Cohen JD,Saftner RA

    更新日期:1995-11-01 00:00:00

  • Cloning and functional expression of a cytochrome P450 cDNA encoding 2-hydroxyisoflavanone synthase involved in biosynthesis of the isoflavonoid skeleton in licorice.

    abstract::Isoflavonoids are distributed predominantly in leguminous plants and play critical roles in plant physiology. A cytochrome P450 (P450), 2-hydroxyisoflavanone synthase, is the key enzyme in their biosynthesis. In cultured licorice (Glycyrrhiza echinata L., Fabaceae) cells, the production of both an isoflavonoid-derived...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Akashi T,Aoki T,Ayabe Si

    更新日期:1999-11-01 00:00:00