Abscission: the role of RNA synthesis.

Abstract:

:Ethylene stimulated the incorporation of (32)P into RNA in the abscission zone of bean explants (Phaseolus vulgaris L. var. Red Kidney). The enhancement was observed in all fractions separated by methylated albumin kieselguhr column chromatography, although the magnitude of the increase was not the same for each fraction. Differential extraction of the nucleic acids indicated that the ethylene stimulation was confined to the fraction extracted with sodium lauryl sulfate, with the increase mainly in Fraction III (Ribosomal RNA) and Fraction IV (Messenger RNA). Actinomycin D, which blocks ethylene-stimulated abscission, inhibited (32)P incorporation into all column fractions. 5-Fluorouracil, which blocked 50% of the ethylene-enhanced (32)P incorporation, did not inhibit ethylene-enhanced abscission. The results indicate that ethylene may regulate abscission through control of specific RNA's.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Holm RE,Abeles FB

doi

10.1104/pp.42.8.1094

subject

Has Abstract

pub_date

1967-08-01 00:00:00

pages

1094-102

issue

8

eissn

0032-0889

issn

1532-2548

journal_volume

42

pub_type

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