Root Engineering in Barley: Increasing Cytokinin Degradation Produces a Larger Root System, Mineral Enrichment in the Shoot and Improved Drought Tolerance.

Abstract:

:Root size and architecture are important crop plant traits, as they determine access to water and soil nutrients. The plant hormone cytokinin is a negative regulator of root growth and branching. Here, we generated transgenic barley (Hordeum vulgare) plants with an enlarged root system by enhancing cytokinin degradation in roots to explore the potential of cytokinin modulations in improving root functions. This was achieved through root-specific expression of a CYTOKININ OXIDASE/DEHYDROGENASE gene. Enhanced biomass allocation to roots did not penalize shoot growth or seed yield, indicating that these plants were not source limited. In leaves of transgenic lines, the concentrations of several macroelements and microelements were increased, particularly those with low soil mobility (phosphorus, manganese, and zinc). Importantly, seeds contained up to 44% more zinc, which is beneficial for human nutrition. Transgenic lines also demonstrated dampened stress responses to long-term drought conditions, indicating lower drought sensitivity. Taken together, this work demonstrates that root engineering of cereals is a promising strategy to improve nutrient efficiency, biofortification, and drought tolerance.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Ramireddy E,Hosseini SA,Eggert K,Gillandt S,Gnad H,von Wirén N,Schmülling T

doi

10.1104/pp.18.00199

subject

Has Abstract

pub_date

2018-07-01 00:00:00

pages

1078-1095

issue

3

eissn

0032-0889

issn

1532-2548

pii

pp.18.00199

journal_volume

177

pub_type

杂志文章
  • A transcriptome-based characterization of habituation in plant tissue culture.

    abstract::For the last 50 years, scientists have recognized that varying ratios of the plant hormones cytokinin and auxin induce plant cells to form particular tissues: undifferentiated calli, shoot structures, root structures, or a whole plant. Proliferation of undifferentiated callus tissue, greening, and the formation of sho...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.076059

    authors: Pischke MS,Huttlin EL,Hegeman AD,Sussman MR

    更新日期:2006-04-01 00:00:00

  • Long-Distance Water Transport in Aquatic Plants.

    abstract::Acropetal mass flow of water is demonstrated in two submerged angiosperms, Lobelia dortmanna L. and Sparganium emersum Rehman by means of guttation measurements. Transpiration is absent in truly submerged plants, but the presence of guttation verifies that long-distance water transport takes place. Use of tritiated wa...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.4.1369

    authors: Pedersen O

    更新日期:1993-12-01 00:00:00

  • Enhancement of carotenoid biosynthesis in transplastomic tomatoes by induced lycopene-to-provitamin A conversion.

    abstract::Carotenoids are essential pigments of the photosynthetic apparatus and an indispensable component of the human diet. In addition to being potent antioxidants, they also provide the vitamin A precursor beta-carotene. In tomato (Solanum lycopersicum) fruits, carotenoids accumulate in specialized plastids, the chromoplas...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.140533

    authors: Apel W,Bock R

    更新日期:2009-09-01 00:00:00

  • The Pepper RING-Type E3 Ligase CaAIRF1 Regulates ABA and Drought Signaling via CaADIP1 Protein Phosphatase Degradation.

    abstract::Ubiquitin-mediated protein modification occurs at multiple steps of abscisic acid (ABA) signaling. Here, we sought proteins responsible for degradation of the pepper (Capsicum annuum) type 2C protein phosphatase CaADIP1 via the 26S proteasome system. We showed that the RING-type E3 ligase CaAIRF1 (Capsicum annuum ADIP...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01817

    authors: Lim CW,Baek W,Lee SC

    更新日期:2017-04-01 00:00:00

  • Transfer of oligosaccharide to protein from a lipid intermediate in plants.

    abstract::A lipid-bound oligosaccharide was isolated from pea (Pisum sativum) cotyledons incubated with [(14)C]mannose. The oligosaccharide moiety appeared to be identical with the one obtained from rat liver, known to contain three glucoses, nine mannoses, and two N-acetylglucosamines, and to be involved in protein glycosylati...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.5.1175

    authors: Staneloni RJ,Tolmasky ME,Petriella C,Leloir LF

    更新日期:1981-11-01 00:00:00

  • Transcriptome analysis reveals a critical role of CHS7 and CHS8 genes for isoflavonoid synthesis in soybean seeds.

    abstract::We have used cDNA microarray analysis to examine changes in gene expression during embryo development in soybean (Glycine max) and to compare gene expression profiles of two soybean cultivars that differ in seed isoflavonoid content. The analysis identified 5,910 genes that were differentially expressed in both soybea...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.086306

    authors: Dhaubhadel S,Gijzen M,Moy P,Farhangkhoee M

    更新日期:2007-01-01 00:00:00

  • CfrA, a Novel Carbon Flow Regulator, Adapts Carbon Metabolism to Nitrogen Deficiency in Cyanobacteria.

    abstract::Cyanobacteria unable to fix atmospheric nitrogen have evolved sophisticated adaptations to survive to long periods of nitrogen starvation. These genetic programs are still largely unknown-as evidenced by the many proteins whose expression is regulated in response to nitrogen availability, but which belong to unknown o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00802

    authors: Muro-Pastor MI,Cutillas-Farray Á,Pérez-Rodríguez L,Pérez-Saavedra J,Vega-de Armas A,Paredes A,Robles-Rengel R,Florencio FJ

    更新日期:2020-12-01 00:00:00

  • Purification and characterization of the reconstitutively active citrate carrier from maize mitochondria.

    abstract::The citrate carrier from maize (Zea mays) shoot mitochondria was solubilized with Triton X-100 and purified by sequential chromatography on hydroxyapatite and hydroxyapatite/celite in the presence of cardiolipin. SDS-gel electrophoresis of the purified fraction showed a single polypeptide band with an apparent molecul...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.120.3.841

    authors: Genchi G,Spagnoletta A,De Santis A,Stefanizzi L,Palmieri F

    更新日期:1999-07-01 00:00:00

  • The Golgi Localization of GnTI Requires a Polar Amino Acid Residue within Its Transmembrane Domain.

    abstract::The Golgi apparatus consists of stacked cisternae filled with enzymes that facilitate the sequential and highly controlled modification of glycans from proteins that transit through the organelle. Although the glycan processing pathways have been extensively studied, the underlying mechanisms that concentrate Golgi-re...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00310

    authors: Schoberer J,Liebminger E,Vavra U,Veit C,Grünwald-Gruber C,Altmann F,Botchway SW,Strasser R

    更新日期:2019-06-01 00:00:00

  • Root Type-Specific Reprogramming of Maize Pericycle Transcriptomes by Local High Nitrate Results in Disparate Lateral Root Branching Patterns.

    abstract::The adaptability of root system architecture to unevenly distributed mineral nutrients in soil is a key determinant of plant performance. The molecular mechanisms underlying nitrate dependent plasticity of lateral root branching across the different root types of maize are only poorly understood. In this study, detail...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.01885

    authors: Yu P,Baldauf JA,Lithio A,Marcon C,Nettleton D,Li C,Hochholdinger F

    更新日期:2016-03-01 00:00:00

  • Identification of methyl jasmonate and salicylic acid response elements from the nopaline synthase (nos) promoter.

    abstract::Transgenic tobacco plants carrying a fusion between the nopaline synthase (nos) promoter and chloramphenicol acetyltransferase (CAT) reporter gene (cat) were studied for their inducibility by salicylic acid (SA) or methyl jasmonate (MJ) treatments. Either chemical significantly increased CAT activity to a level much h...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.1.97

    authors: Kim SR,Kim Y,An G

    更新日期:1993-09-01 00:00:00

  • Next-generation systemic acquired resistance.

    abstract::Systemic acquired resistance (SAR) is a plant immune response to pathogen attack. Recent evidence suggests that plant immunity involves regulation by chromatin remodeling and DNA methylation. We investigated whether SAR can be inherited epigenetically following disease pressure by Pseudomonas syringae pv tomato DC3000...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.187468

    authors: Luna E,Bruce TJ,Roberts MR,Flors V,Ton J

    更新日期:2012-02-01 00:00:00

  • Localization of [gamma]-Glutamylcysteine Synthetase and Glutathione Synthetase Activity in Maize Seedlings.

    abstract::Fresh weight, protein, cysteine, [gamma]-glutamylcysteine, glutathione, and the extractable activity of the enzymes of glutathione biosynthesis, [gamma]-glutamylcysteine synthetase (EC 6.3.2.2) and glutathione synthetase (EC 6.3.2.3), were measured in roots, scutella, endosperms, and shoots of 3-, 7-, and 11-d-old mai...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.2.561

    authors: Ruegsegger A,Brunold C

    更新日期:1993-02-01 00:00:00

  • Control of specific gene expression by gibberellin and brassinosteroid.

    abstract::We identified a recessive, brassinolide-insensitive mutant caused by a deletion allele (bri1-201) of the brassinosteroid (BR) receptor BRI1. The bri1-201 mutant displayed altered expression levels of genes differentially regulated by gibberellin (GA). RNA-blot analysis revealed that BR and GA antagonistically regulate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:

    authors: Bouquin T,Meier C,Foster R,Nielsen ME,Mundy J

    更新日期:2001-10-01 00:00:00

  • The arabidopsis cell plate-associated dynamin-like protein, ADL1Ap, is required for multiple stages of plant growth and development.

    abstract::Dynamin and dynamin-like proteins are GTP-binding proteins involved in vesicle trafficking. In soybean, a 68-kD dynamin-like protein called phragmoplastin has been shown to be associated with the cell plate in dividing cells (Gu and Verma, 1996). Five ADL1 genes encoding dynamin-like proteins related to phragmoplastin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.126.1.47

    authors: Kang BH,Busse JS,Dickey C,Rancour DM,Bednarek SY

    更新日期:2001-05-01 00:00:00

  • Preparation of Corn Root Plasmalemma with Low Mg-ATPase Latency and High Electrogenic H Pumping Activity after Phase Partitioning.

    abstract::Crude plasma membranes of corn (Zea mays L.) roots were obtained according to MI De Michelis and RM Spanswick (1986 Plant Physiol 81: 542-547). This preparation, which contained tightly sealed vesicles displaying Mg-ATP dependent H(+)-transport, was purified by phase partitioning. The percentage of inside-out vesicles...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.2.491

    authors: Galtier N,Belver A,Gibrat R,Grouzis JP,Rigaud J,Grignon C

    更新日期:1988-06-01 00:00:00

  • The Azolla-Anabaena azollae Relationship : XI. PHYCOBILIPROTEINS IN THE ACTION SPECTRUM FOR NITROGENASE-CATALYZED ACETYLENE REDUCTION.

    abstract::Visible absorption spectra are presented for the Azolla caroliniana Willd.-Anabaena azollae Strass. association and the individual partners. Although absorption by the phycobiliproteins of the endophytic cyanobacterium clearly complements the absorption by the fern pigments, their contribution to the absorption spectr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.6.1479

    authors: Tyagi VV,Ray TB,Mayne BC,Peters GA

    更新日期:1981-12-01 00:00:00

  • Inhibition of Flowering of Xanthium pensylvanicum Wallr. by Prolonged Irradiation with Far Red.

    abstract::Interrupting each long night with a prolonged period of far red radiant energy resulted in the inhibition of floral initiation in cocklebur. Irradiations inducing different relative levels of P(FR) from 1 to 2% to 80% had about the same effect under 4-hour photoperiods. The lower levels of P(FR) induced by continuous ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.1.95

    authors: Mancinelli AL,Downs RJ

    更新日期:1967-01-01 00:00:00

  • Unraveling transcriptional control in Arabidopsis using cis-regulatory elements and coexpression networks.

    abstract::Analysis of gene expression data generated by high-throughput microarray transcript profiling experiments has demonstrated that genes with an overall similar expression pattern are often enriched for similar functions. This guilt-by-association principle can be applied to define modular gene programs, identify cis-reg...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.136028

    authors: Vandepoele K,Quimbaya M,Casneuf T,De Veylder L,Van de Peer Y

    更新日期:2009-06-01 00:00:00

  • Root Exudates of Various Host Plants of Rhizobium leguminosarum Contain Different Sets of Inducers of Rhizobium Nodulation Genes.

    abstract::Rhizobium promoters involved in the formation of root nodules on leguminous plants are activated by flavonoids in plant root exudate. A series of Rhizobium strains which all contain the inducible Rhizobium leguminosarum nodA promoter fused to the Escherichia coli lacZ gene, and which differ only in the source of the r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.4.1298

    authors: Zaat SA,Wijffelman CA,Mulders IH,van Brussel AA,Lugtenberg BJ

    更新日期:1988-04-01 00:00:00

  • A soybean dual-specificity kinase, GmSARK, and its Arabidopsis homolog, AtSARK, regulate leaf senescence through synergistic actions of auxin and ethylene.

    abstract::As the last stage of leaf development, senescence is a fine-tuned process regulated by interplays of multiple signaling pathways. We have previously identified soybean (Glycine max) SENESCENCE-ASSOCIATED RECEPTOR-LIKE KINASE (SARK), a leucine-rich repeat-receptor-like protein kinase from soybean, as a positive regulat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.182899

    authors: Xu F,Meng T,Li P,Yu Y,Cui Y,Wang Y,Gong Q,Wang NN

    更新日期:2011-12-01 00:00:00

  • Expression and molecular analysis of the Arabidopsis DXR gene encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase, the first committed enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway.

    abstract::1-Deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) catalyzes the first committed step of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis. In Arabidopsis, DXR is encoded by a single-copy gene. We have cloned a full-length cDNA corresponding to this gene. A comparative analysis of all plan...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.003798

    authors: Carretero-Paulet L,Ahumada I,Cunillera N,Rodríguez-Concepción M,Ferrer A,Boronat A,Campos N

    更新日期:2002-08-01 00:00:00

  • Regulation of Sucrose Transporters and Phloem Loading in Response to Environmental Cues.

    abstract::Suc transporters (SUTs) play a key role in the allocation and partitioning of photosynthetically fixed carbon in plants. While a function could be assigned to many members of the SUT family, almost no information is available on their regulation. Here, the transcriptional regulation of SUTs in response to various envi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.01088

    authors: Xu Q,Chen S,Yunjuan R,Chen S,Liesche J

    更新日期:2018-01-01 00:00:00

  • Partial purification of gibberellin oxidases from spinach leaves.

    abstract::Four enzyme activities catalyzing the following oxidative steps in the gibberellin (GA) biosynthetic pathway have been extracted from spinach (Spinacia oleracea L.) leaves after exposure to 8 long days: GA(12) --> GA(53) --> GA(44) --> GA(19) --> GA(20). Two of these, GA(53) oxidase and GA(19) oxidase, were separable ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.1.87

    authors: Gilmour SJ,Bleecker AB,Zeevaart JA

    更新日期:1987-09-01 00:00:00

  • Chemical Composition of Hypodermal and Endodermal Cell Walls and Xylem Vessels Isolated from Clivia miniata (Identification of the Biopolymers Lignin and Suberin).

    abstract::The occurrence of the biopolymers lignin and suberin was investigated with hypodermal (HCW) and endodermal cell walls (ECW) and xylem vessels (XV) isolated from Clivia miniata Reg. roots. Both biopolymers were detected in HCW and ECW, whereas in XV, typical aliphatic suberin monomers were missing and only representati...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.4.1223

    authors: Zeier J,Schreiber L

    更新日期:1997-04-01 00:00:00

  • Sequences downstream of the translation initiation codon are important determinants of translation efficiency in chloroplasts.

    abstract::The objective of this study was to determine if mRNA sequences downstream of the translation initiation codon are important for translation of plastid mRNAs. We have employed a transgenic approach, measuring accumulation of the neomycin phosphotransferase (NPTII) reporter enzyme translationally fused with 14 N-termina...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.125.1.430

    authors: Kuroda H,Maliga P

    更新日期:2001-01-01 00:00:00

  • Spatial and temporal distribution of polyamine levels and polyamine anabolism in different organs/tissues of the tobacco plant. Correlations with age, cell division/expansion, and differentiation.

    abstract::Polyamine (PA) titers and biosynthesis follow a basipetal decrease along the tobacco (Nicotiana tabacum) plant axis, and they also correlate negatively with cell size. On the contrary, the titers of arginine (Arg), ornithine (Orn), and arginase activity increase with age. The free (soluble)/total-PA ratios gradually i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.055483

    authors: Paschalidis KA,Roubelakis-Angelakis KA

    更新日期:2005-05-01 00:00:00

  • Synthesis and Interconversion of Amino Acids in Developing Cotyledons of Pea (Pisum sativum L.).

    abstract::Freshly isolated cotyledons from 10-day developing pea (Pisum sativum) seeds were fed radiolabeled precursors for 5 hours, and the specific radioactivity of the free and total protein amino acids was determined using a dansylation procedure. When the seven most abundant amino acids in phloem exudate of pea fruits (asp...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.3.344

    authors: Macnicol PK

    更新日期:1977-09-01 00:00:00

  • Purification and Characterization of an Endophytic Fungal Proteinase That Is Abundantly Expressed in the Infected Host Grass.

    abstract::A novel Acremonium typhinum proteinase that is expressed during endophytic infection of the grass Poa ampla Merr. was purified from endophyte-infected leaf sheath tissue. It is a thiol-containing serine alkaline endoproteinase with bound carbohydrate. In the infected host tissue, this proteinase is an abundant protein...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.1.7

    authors: Lindstrom JT,Belanger FC

    更新日期:1994-09-01 00:00:00

  • Differential Response in the Water Status of Immature and Mature Fronds of the Ostrich Fern (Matteuccia struthiopteris [L.] Todaro) to a Mild Water Stress.

    abstract::Experiments were conducted in growth chambers to examine the effect of a mild water stress (-200 kilopascals polyethylene glycol) on frond elongation and water status of the ostrich fern (Matteuccia struthiopteris [L.] Todaro). Measurements were taken for two days, starting one day after the application of polyethylen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.1.96

    authors: Prange RK,Ormrod DP

    更新日期:1983-05-01 00:00:00