Abstract:
:Transgenic tobacco plants carrying a fusion between the nopaline synthase (nos) promoter and chloramphenicol acetyltransferase (CAT) reporter gene (cat) were studied for their inducibility by salicylic acid (SA) or methyl jasmonate (MJ) treatments. Either chemical significantly increased CAT activity to a level much higher than that achieved by wounding. Northern blot analysis showed a corresponding increase in mRNA levels. After 20 h of induction of flowering plants, the response to MJ treatment was weaker in old leaves compared with young leaves, whereas the SA response was stronger in old leaves. Kinetic experiments showed that the SA response was much faster than the MJ response, suggesting that the induction mechanism of the nos promoter by these chemicals may differ. Deletion analysis showed that both SA and MJ responses require the DNA sequence between -119 and -112 from the transcription initiation site. This region contains the hexamer sequence (TGACGT) that has been found to be an important regulatory element for several promoters. The MJ response was also reduced by deletions of the CAAT box region or the sequence between -112 and -101, whereas the SA response was not significantly affected by these deletions. This suggests that the nos upstream region containing the hexamer motif is essential for the SA or MJ response and that the CAAT box region and the sequence immediately downstream from the hexamer motif are required for maximum induction by MJ.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Kim SR,Kim Y,An Gdoi
10.1104/pp.103.1.97subject
Has Abstractpub_date
1993-09-01 00:00:00pages
97-103issue
1eissn
0032-0889issn
1532-2548pii
103/1/97journal_volume
103pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Two-dimensional electrophoresis was performed on proteins of bundle sheath and mesophyll cells isolated from the C(4) grass Digitaria sanguinalis (L.) Scop. Two-dimensional maps of these proteins were constructed and ribulose-1,5-biphosphate carboxylase and phosphoenolpyruvate carboxylase were identified. Of the total...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.5.1359
更新日期:1982-11-01 00:00:00
abstract::Branched-chain amino acids (BCAAs) are synthesized in plants from branched-chain keto acids, but their metabolism is not completely understood. The interface of BCAA metabolism lies with branched-chain aminotransferases (BCAT) that catalyze both the last anabolic step and the first catabolic step. In this study, six B...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.154922
更新日期:2010-07-01 00:00:00
abstract::The cereal aleurone layer is a model system for studying the regulation of transcription by gibberellin (GA) and abscisic acid (ABA). GA stimulates and ABA prevents the transcription of genes for alpha-amylases and other secreted hydrolytic enzymes, but how GA and ABA affect the transcription of other genes is largely...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.074435
更新日期:2006-02-01 00:00:00
abstract::During phosphate starvation, Snf1-related kinase 1 (SnRK1) activity significantly decreases compared with plants growing under normal nutritional conditions. An analysis of the expression of the genes encoding for the catalytic subunits of SnRK1 showed that these subunits were not affected by phosphate starvation. Tra...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.133298
更新日期:2009-04-01 00:00:00
abstract::Sections of sugar beet (Beta vulgaris L.) taproot were incubated in various concentrations of mannitol. At 0.4, 0.6, and 0.8 molar, the membrane electrical potential difference (E(m)) averaged about -130 millivolts; at 0.2 molar, about -90 millivolts; and at 0 molar, between -60 and -80 millivolts. Additions of 10 mil...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.82.4.1148
更新日期:1986-12-01 00:00:00
abstract::The DELLA protein REPRESSOR OF ga1-3-LIKE2 (RGL2) plays an important role in seed germination under different conditions through a number of transcription factors. However, the functions of the structural genes associated with RGL2-regulated germination are less defined. Here, we report the role of an Arabidopsis (Ara...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00858
更新日期:2015-11-01 00:00:00
abstract::Systemic acquired resistance (SAR) is a plant immune response to pathogen attack. Recent evidence suggests that plant immunity involves regulation by chromatin remodeling and DNA methylation. We investigated whether SAR can be inherited epigenetically following disease pressure by Pseudomonas syringae pv tomato DC3000...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.187468
更新日期:2012-02-01 00:00:00
abstract::alpha-Amylase activity increased in attached cotyledons of germinated Vigna mungo seeds until the 5th day after imbibition and decreased thereafter, whereas in detached and incubated cotyledons the activity continuously increased and, at the 6th day, reached the value more than three times that of the maximum activity...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.71.1.173
更新日期:1983-01-01 00:00:00
abstract::The initial rate of Ca2+ movement across the inner-envelope membrane of pea (Pisum sativum L.) chloroplasts was directly measured by stopped-flow spectrofluorometry using membrane vesicles loaded with the Ca2+-sensitive fluorophore fura-2. Calibration of fura-2 fluorescence was achieved by combining a ratiometric meth...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.118.4.1447
更新日期:1998-12-01 00:00:00
abstract::A water-soluble chlorophyll protein (WSCP) in cauliflower (Brassica oleracea L.) was purified and its N-terminal sequence was determined. Forty-six of 48 residues of the sequence completely matched those of the drought-induced 22-kD protein (BnD22) in rapeseed (Brassica napus L.). All 40 sequenced residues of WSCP fro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.115.2.841
更新日期:1997-10-01 00:00:00
abstract::To examine the role of isoamylase1 (ISA1) in amylopectin biosynthesis in plants, a genomic DNA fragment from Aegilops tauschii was introduced into the ISA1-deficient rice (Oryza sativa) sugary-1 mutant line EM914, in which endosperm starch is completely replaced by phytoglycogen. A. tauschii is the D genome donor of w...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.051359
更新日期:2005-01-01 00:00:00
abstract::A population of M2 seedlings of Arabidopsis thaliana was screened for mutants that were insensitive to the ethylene precursor 1-aminocyclopropane-1-carboxylate (ACC). Several independent lines were obtained and proved insensitive to both ACC and ethylene. Two lines were identified as alleles of a single recessive muta...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.2.401
更新日期:1993-06-01 00:00:00
abstract::When fully filled pods of bean plants were deseeded, the rate of axillary bud growth and the chlorophyll content of leaves were increased. Application of 0.1% indoleacetic acid (IAA) in lanolin on the deseeded pods caused abscission of axillary buds, inhibited growth of the remaining buds, and decreased leaf chlorophy...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.2.476
更新日期:1981-08-01 00:00:00
abstract::In this study, we generated transgenic Arabidopsis (Arabidopsis thaliana) plants overexpressing the Rieske FeS protein (PetC), a component of the cytochrome b6f (cyt b6f) complex. Increasing the levels of this protein resulted in concomitant increases in the levels of cyt f (PetA) and cyt b6 (PetB), core proteins of t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00622
更新日期:2017-09-01 00:00:00
abstract::Seven-day-old dark-grown bean leaves were greened under continuous light. The amount of chlorophyll, the ratio of chlorophyll a to chlorophyll b, the O(2) evolving capacity and the primary photochemical activities of Photosystem I and Photosystem II were measured on the leaves after various times of greening. The prim...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.58.4.526
更新日期:1976-10-01 00:00:00
abstract::Chloroplast division is driven by a macromolecular complex containing components that are positioned on the cytosolic surface of the outer envelope, the stromal surface of the inner envelope, and in the intermembrane space. The only constituents of the division apparatus identified thus far are the tubulin-like protei...
journal_title:Plant physiology
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
abstract::In red clover (Trifolium pratense) leaves, phaselic acid (2-O-caffeoyl-L-malate) accumulates to several mmol kg(-1) fresh weight and is a crucial component of a natural system that prevents protein breakdown during harvest and storage of this forage crop. Previously, we identified HCT2, a red clover gene encoding a hy...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.166793
更新日期:2011-03-01 00:00:00
abstract::Wheat (Triticum aestivum L. cv Etoile de Choisy) microsomes catalyzed the cytochrome P-450-dependent oxidation of the herbicide diclofop to three hydroxy-diclofop isomers. Hydroxylation was predominant at carbon 4, with migration of chlorine to carbon 5 (67%) and carbon 3 (25%). The 2,4-dichloro-5-hydroxy isomer was i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.2.874
更新日期:1992-10-01 00:00:00
abstract::Inactivation of inward-rectifying K+ channels (IK,in) by a rise in cytosolic free [Ca2+] ([Ca2+]i) is a key event leading to solute loss from guard cells and stomatal closure. However, [Ca2+]i action on IK,in has never been quantified, nor are its origins well understood. We used membrane voltage to manipulate [Ca2+]i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.119.1.277
更新日期:1999-01-01 00:00:00
abstract::The fate of glutamine, the major source of nitrogen supplied to the developing maize endosperm, has been examined in endosperm tissues of corn caryopsis grown under sterile conditions. In the culture system, [U-(14)C]glutamine was included in the medium or was injected directly into the endosperm. Samples were harvest...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.77.3.520
更新日期:1985-03-01 00:00:00
abstract::The pbs3-1 mutant, identified in a screen for Arabidopsis (Arabidopsis thaliana) mutants exhibiting enhanced susceptibility to the avirulent Pseudomonas syringae pathogen DC3000 (avrPphB), also exhibits enhanced susceptibility to virulent P. syringae strains, suggesting it may impact basal disease resistance. Because ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.097691
更新日期:2007-06-01 00:00:00
abstract::The possibility of HCO(3) (-) transport into isolated leaf mesophyll cells of Asparagus sprengeri Regel has been investigated. Measurement of the inorganic carbon pool in these cells over an external pH range 6.2 to 8.0, using the silicone-fluid filtration technique, indicated that the pool was larger than predicted b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.3.649
更新日期:1982-09-01 00:00:00
abstract::Very low ethylene production rates were measured in nonpollinated Cyclamen persicum Mill flowers, and no change in production was observed during the whole life span of the flower until death. Normal senescence was accompanied by a gradual discoloration and loss of turgor followed by wilting. Pollination induced a dra...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.4.1090
更新日期:1984-08-01 00:00:00
abstract::A closed system was designed for concomitant determination of net CO(2) uptake, transpiration, and foliar nitrogen (N) loss in soybeans (Glycine max [L.] Merr.). The CO(2) uptake was monitored by infrared analysis of system air removed periodically in aliquots. Leaf vapors were trapped in calibrated tubes at Dry Ice t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.65.2.403
更新日期:1980-02-01 00:00:00
abstract::The effect of the exogenous and endogenous NO(3) (-) concentration on net uptake, influx, and efflux of NO(3) (-) and on nitrate reductase activity (NRA) in roots was studied in Phaseolus vulgaris L. cv. Witte Krombek. After exposure to NO(3) (-), an apparent induction period of about 6 hours occurred regardless of th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.3.754
更新日期:1982-09-01 00:00:00
abstract::The first committed step in the conversion of cycloartenol into Delta(5) C24-alkyl sterols in plants is catalyzed by an S-adenosyl-methionine-dependent sterol-C24-methyltransferase type 1 (SMT1). We report the consequences of overexpressing SMT1 in tobacco (Nicotiana tabacum), under control of either the constitutive ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.004226
更新日期:2002-09-01 00:00:00
abstract::Experiments were conducted in growth chambers to examine the effect of a mild water stress (-200 kilopascals polyethylene glycol) on frond elongation and water status of the ostrich fern (Matteuccia struthiopteris [L.] Todaro). Measurements were taken for two days, starting one day after the application of polyethylen...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.72.1.96
更新日期:1983-05-01 00:00:00
abstract::A double pressure probe technique was used to measure simultaneously water flows and hydraulic parameters of individual cells and of excised roots of young seedlings of maize (Zea mays L.) in osmotic experiments. By following initial flows of water at the cell and root level and by estimating the profiles of driving f...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.95.1.305
更新日期:1991-01-01 00:00:00
abstract::The slow inactivation which accompanies catalysis by higher-plant ribulose-P(2) carboxylase-oxygenase (Rubisco) in vitro was only partially reversed when the enzyme was gel filtered to remove small molecules. However, gel filtration or dialysis in the presence of high SO(2-) (4) concentrations induced full recovery. T...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.4.1390
更新日期:1990-08-01 00:00:00
abstract::Lipid peroxide-derived toxic carbonyl compounds (oxylipin carbonyls), produced downstream of reactive oxygen species (ROS), were recently revealed to mediate abiotic stress-induced damage of plants. Here, we investigated how oxylipin carbonyls cause cell death. When tobacco (Nicotiana tabacum) Bright Yellow-2 (BY-2) c...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.115.256834
更新日期:2015-07-01 00:00:00