Control of specific gene expression by gibberellin and brassinosteroid.

Abstract:

:We identified a recessive, brassinolide-insensitive mutant caused by a deletion allele (bri1-201) of the brassinosteroid (BR) receptor BRI1. The bri1-201 mutant displayed altered expression levels of genes differentially regulated by gibberellin (GA). RNA-blot analysis revealed that BR and GA antagonistically regulate the accumulation of mRNAs of the GA-responsive GASA1 gene, as well as the GA-repressible GA5 gene. Expression studies with cycloheximide indicated that the antagonistic effects of GA and BR on GA5 require de novo protein synthesis. Reporter transgene analyses and RNA-blot analysis showed that BR and GA modulate GA5 expression, at least in part, at the transcriptional level, and that the signals are independent and subtractive.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Bouquin T,Meier C,Foster R,Nielsen ME,Mundy J

subject

Has Abstract

pub_date

2001-10-01 00:00:00

pages

450-8

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

127

pub_type

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