Abstract:
:We identified a recessive, brassinolide-insensitive mutant caused by a deletion allele (bri1-201) of the brassinosteroid (BR) receptor BRI1. The bri1-201 mutant displayed altered expression levels of genes differentially regulated by gibberellin (GA). RNA-blot analysis revealed that BR and GA antagonistically regulate the accumulation of mRNAs of the GA-responsive GASA1 gene, as well as the GA-repressible GA5 gene. Expression studies with cycloheximide indicated that the antagonistic effects of GA and BR on GA5 require de novo protein synthesis. Reporter transgene analyses and RNA-blot analysis showed that BR and GA modulate GA5 expression, at least in part, at the transcriptional level, and that the signals are independent and subtractive.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Bouquin T,Meier C,Foster R,Nielsen ME,Mundy Jsubject
Has Abstractpub_date
2001-10-01 00:00:00pages
450-8issue
2eissn
0032-0889issn
1532-2548journal_volume
127pub_type
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