Abstract:
:Exposure of dark grown resting Euglena to ethanol produced a transient increase in the specific activity of the glyoxysomal enzyme malate synthase. Enzyme specific activity increased during the first 24 hours of ethanol treatment and then declined. Light exposure or malate addition failed to increase enzyme specific activity. The increase and decrease in enzyme specific activity represented changes in the amount of active enzyme. In both wild type cells and the plastidless mutant W(3)BUL, enzyme levels were always higher in the dark than in the light.The specific activity of the peroxisomal enzyme glycolate dehydrogenase began to increase 24 hours after dark grown resting Euglena were exposed to light. Ethanol, but not malate, prevented the increase and promoted a decrease in glycolate dehydrogenase levels. Cycloheximide produced a decline in enzyme levels similar to the decline produced by ethanol addition. Glycolate dehydrogenase was present in the plastidless mutant W(3)BUL indicating that it is coded in the nucleus and synthesized on cytoplasmic ribosomes. Streptomycin, a specific inhibitor of chloroplast protein synthesis and 3-(3,4-dichlorophenyl)-1,1-dimethylurea, an inhibitor of photosynthetic CO(2) fixation, inhibited the photoinduction of glycolate dehydrogenase while having no effect on the photoinduction of NADP dependent glyceraldehyde-3-phosphate dehydrogenase, another light induced, nuclear coded, cytoplasmically synthesized enzyme. Taken together, these results suggest that microbodies are continually synthesized in resting Euglena and their enzyme complement is determined through substrate induction of glyoxysomal and peroxisomal enzymes.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Horrum MA,Schwartzbach SDdoi
10.1104/pp.68.2.430subject
Has Abstractpub_date
1981-08-01 00:00:00pages
430-4issue
2eissn
0032-0889issn
1532-2548journal_volume
68pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::A simplified procedure (involving disruption in sorbitol-pyrophosphate mixtures) permits the separation of spinach chloroplasts which retain the ability to catalyze the photosynthetic assimilation of carbon dioxide and its associated oxygen evolution. ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.9.1415
更新日期:1968-09-01 00:00:00
abstract::Sugar beets (Beta vulgaris L.) infected with the Beet Yellows Virus exhibit lower rates of net photosynthesis at light saturation than do healthy plants. These Pn reductions were correlated with increases in leaf resistance to water vapor loss. Theoretical analyses demonstrated that, although the leaf resistance to wa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.50.5.576
更新日期:1972-11-01 00:00:00
abstract::A method is described for the release of large quantities of mesophyll cells from leaves of both cold-hardened and nonhardened winter rye seedlings by a combination of enzymic and physical maceration. Such preparations usually contain a certain percentage of nonviable cells and are thus not suitable for quantitative b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.5.906
更新日期:1981-05-01 00:00:00
abstract::The nature of bound forms of enzymes of phenylpropanoid and flavonoid metabolism have been investigated in Hippeastrum CV Dutch Red Hybrid. Particulate components of petal homogenates were fractionated on sucrose gradients and the EDTA shift method was employed to characterize membranes of the endoplasmic reticulum. I...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.74.4.901
更新日期:1984-04-01 00:00:00
abstract::The large ATP synthase gene cluster in spinach (Spinacia oleracea) plastids encodes four of the six chloroplast-encoded ATP synthase subunits. Expression of this cluster was examined to determine its response to light-induced plastid development. Spinach plastid transcripts were isolated from etiolated tissues, etiola...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.3.1164
更新日期:1992-11-01 00:00:00
abstract::The success of plant reproduction depends on pollen-pistil interactions occurring at the stigma/style. These interactions vary depending on the stigma type: wet or dry. Tobacco (Nicotiana tabacum) represents a model of wet stigma, and its stigmas/styles express genes to accomplish the appropriate functions. For a larg...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.131573
更新日期:2009-03-01 00:00:00
abstract::The carotenoid content of thylakoids and envelopes isolated from dark-or light-treated spinach (Spinacia oleracea L.) chloroplasts was compared. In thylakoids, light induced a decrease of violaxanthin parallel with a stoichiometric increase of zeaxanthin due to violaxanthin deepoxidation. In envelopes, violaxanthin wa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.61.4.530
更新日期:1978-04-01 00:00:00
abstract::Vesicular-arbuscular mycorrhizal fungi are symbionts for a large variety of crop plants; however, the form in which they take up carbon from the host is not established. To trace the course of carbon metabolism, we have used nuclear magnetic resonance spectroscopy with [13C]glucose labeling in vivo and in extracts to ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.1.7
更新日期:1995-05-01 00:00:00
abstract::The effect of leaf water deficit on net CO(2) assimilation was studied under two conditions: in one, the stomata were allowed to contribute to the regulation of CO(2) assimilation; in the other, air was forced through the leaf at a constant rate to overcome the effects of change in stomatal resistance accompanying cha...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.55.4.589
更新日期:1975-04-01 00:00:00
abstract::In pea leaves, much of the metabolism of imported asparagine is by transamination. This activity was previously shown to be localized in the peroxisomes, suggesting a possible connection between asparagine and photorespiratory nitrogen metabolism. This was investigated by examination of the transfer of (15)N from the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.2.334
更新日期:1985-06-01 00:00:00
abstract::Changes in the physical state of microsomal membrane lipids during senescence of rose flower petals (Rosa hyb. L. cv Mercedes) were measured by x-ray diffraction analysis. During senescence of cut flowers held at 22 degrees C, lipid in the ordered, gel phase appeared in the otherwise disordered, liquid-crystalline pha...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.83.4.1037
更新日期:1987-04-01 00:00:00
abstract::Using novel specially designed instrumentation, fluorescence emission spectra were recorded from Arabidopsis (Arabidopsis thaliana) leaves during the induction period of dark to high-light adaptation in order to follow the spectral changes associated with the formation of nonphotochemical quenching. In addition to an ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.148213
更新日期:2010-03-01 00:00:00
abstract::Cell-free extracts of Chlorella pyrenoidosa contained two enzymes capable of oxidizing d-lactate; these were glycolate dehydrogenase and NAD(+)-dependent d-lactate dehydrogenase. The two enzymes could be distinguished by differential centrifugation, glycolate dehydrogenase being largely particulate and NAD(+)-d-lactat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.53.2.167
更新日期:1974-02-01 00:00:00
abstract::The petunia (Petunia [Mitchell]) chloroplast proteins, the chlorophyll a/b-binding (Cab) proteins, and the small subunit of ribulose bisphosphate carboxylase (RbcS) are encoded by nuclear genes that are expressed in a light-dependent manner. The steady-state concentrations of five cab mRNAs vary with a dramatic circad...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.3.776
更新日期:1989-03-01 00:00:00
abstract::The ability of the wheat germ initiation factors and ribosomes to serve as substrates for a wheat germ protein kinase (Yan and Tao 1982 J Biol Chem 257: 7037-7043) has been investigated. The wheat germ kinase catalyzes the phosphorylation of the 42,000 dalton subunit of eukaryotic initiation factor (eIF)-2 and the 107...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.77.2.370
更新日期:1985-02-01 00:00:00
abstract::Microcomplement fixation was employed to compare the immunological differences that occur between purified inhibitor I from potato tubers, the four purified protomers that comprise it, and inhibitor I from tuber and leaf extracts. Total inhibitors of chymotrypsin and trypsin in leaves of seven genera of the Solanaceae...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.50.5.627
更新日期:1972-11-01 00:00:00
abstract::Plant mitochondria contain non-phosphorylating NAD(P)H dehydrogenases (DHs) that are not found in animal mitochondria. The physiological function, substrate specificity, and location of enzymes within this family have yet to be conclusively determined. We have linked genome sequence information to protein and biochemi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.029363
更新日期:2003-12-01 00:00:00
abstract::Thermal conductivity coefficients have been determined for longitudinal and transverse flow in 4 varieties of fresh Citrus wood using steady state-methods. Equations were developed from which thermal conductivity could be rapidly estimated from moisture content or electrical conductivity. The heat balance of large and...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.42.8.1025
更新日期:1967-08-01 00:00:00
abstract::Despite much study of the role of S-adenosylmethionine (SAM) in the methylation of DNA, RNA, and proteins, and as a cofactor for a wide range of biosynthetic processes, little is known concerning the intracellular transport of this essential metabolite. Screening of the Arabidopsis (Arabidopsis thaliana) genome yielde...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.086975
更新日期:2006-11-01 00:00:00
abstract::A vibrating probe was used to measure the changes in ionic currents around gravistimulated roots of Zea mays L. in an effort to determine whether these currents are involved in stimulus transduction from the root cap to the elongation zone. We did not observe a migration of the previously reported auxin-insensitive cu...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.3.1417
更新日期:1992-11-01 00:00:00
abstract::Subtilisin-like proteins represent an ancient family of serine proteases that are extremely widespread in living organisms. We report here the structure and genomic organization of two new transcriptionally active genes encoding proteins that belong to the P69 family of subtilisin-like proteases from tomato (Lycopersi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.122.1.67
更新日期:2000-01-01 00:00:00
abstract::We describe the development and utilization of a new imaging technology for plant biology, optical coherence microscopy (OCM), which allows true in vivo visualization of plants and plant cells. This novel technology allows the direct, in situ (e.g. plants in soil), three-dimensional visualization of cells and events i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.123.1.3
更新日期:2000-05-01 00:00:00
abstract::The unicellular green alga Chlorella kessleri can induce monosaccharide-H+ symport catalyzing the energy-dependent transport of D-glucose (D-Glc) and several other pentoses and hexoses across the plasmalemma. The gene coding for the inducible HUP1 monosaccharide-H+ symporter has been cloned and the protein has been ch...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.1.33
更新日期:1995-01-01 00:00:00
abstract::Intercellular fluid (IF) obtained from tomato (Lycopersicon esculentum L.) leaflets colonized by Cladosporium fulvum Cooke contains specific elicitors that induce necrosis in tomato cultivars resistant to the race of C. fulvum used to produce the IF. The responses of cell-suspension cultures produced from tomato lines...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.99.3.1208
更新日期:1992-07-01 00:00:00
abstract::OsMADS6, an ancient AGAMOUS-LIKE6 (AGL6)-like gene, has essential functions in specifying floral organ and meristem identity in rice (Oryza sativa). However, how AGL6 genes control flower development remains largely unknown. In this study, we report that OsMADS6 directly targets FACTOR OF DNA METHYLATION LIKE 1 (OsFDM...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00017
更新日期:2018-06-01 00:00:00
abstract::Fluxes of mineral nutrients in the xylem are strongly influenced by interactions with the surrounding stem tissues and are probably regulated by them. Toward a mechanistic understanding of these interactions, we applied stable isotope tracers of magnesium, potassium, and calcium continuously to the transpiration strea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.143776
更新日期:2010-02-01 00:00:00
abstract::Thermostability of the photosynthetic apparatus of abscisic acid (ABA)-treated seedlings of barley (Hordeum vulgare) was studied by light-scattering and by fluorescence measurements of isolated chloroplasts. ABA treatment markedly decreased heat damage of the chloroplast ultrastructure; an exogenous ABA concentration ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.4.1228
更新日期:1992-04-01 00:00:00
abstract::Previous work (D.P. Delmer, J. Pear, A. Andrawis, D. Stalker [1995] Mol Gen Genet 248: 43-51) has identified a gene in cotton (Gossypium hirsutum), Rac13, that encodes a small, signal-transducing GTPase and shows high expression in the fiber at the time of transition from primary to secondary wall synthesis. Since Rac...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.4.1491
更新日期:1996-12-01 00:00:00
abstract::Changes in the rate and pattern of protein synthesis and in translatable mRNA population during cold acclimation of alfalfa (Medicago falcata cv Anik) seedlings have been examined. There appears to be a positive correlation between the increase in ability to synthesize proteins at 4 degrees C and the increase in freez...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.84.4.1172
更新日期:1987-08-01 00:00:00
abstract::Some key carotenogenic genes (crts) in Dunaliella bardawil are regulated in response to salt stress partly due to salt-inducible cis-acting elements in their promoters. Thus, we isolated and compared the ζ-carotene desaturase (Dbzds) promoter with other crts promoters including phytoene synthase (Dbpsy), phytoene desa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.235390
更新日期:2014-05-01 00:00:00