Endoplasmic reticulum as a site of phenylpropanoid and flavonoid metabolism in hippeastrum.

Abstract:

:The nature of bound forms of enzymes of phenylpropanoid and flavonoid metabolism have been investigated in Hippeastrum CV Dutch Red Hybrid. Particulate components of petal homogenates were fractionated on sucrose gradients and the EDTA shift method was employed to characterize membranes of the endoplasmic reticulum. In magnesiumcontaining gradients, a portion of phenylalanine ammonia lyase, chalcone synthase, glucosyl transferase, and all of the trans-cinnamate 4-monooxygenase and NADH Cytochrome c reductase (the last an endoplasmic reticulum marker) were associated with membranes equilibrating at 1.18 specific gravity. In gradients lacking magnesium and containing EDTA, the above activities-except chalcone synthase, which was lost-and protein were diminished at 1.18 specific gravity and enhanced at lower densities characteristic of membranes of the smooth endoplasmic reticulum. These results are consistent with the contention that endoplasmic reticulum is a site of phenylpropanoid and flavonoid metabolism in Hippeastrum.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Wagner GJ,Hrazdina G

doi

10.1104/pp.74.4.901

subject

Has Abstract

pub_date

1984-04-01 00:00:00

pages

901-6

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

74

pub_type

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