Fructokinase (Fraction IV) of Pea Seeds.

Abstract:

:A fructokinase (EC 2.7.1.4) was obtained from pea (Pisum sativum L.) seeds. This enzyme, termed fructokinase (fraction IV), was specific for fructose as substrate and had little activity with glucose or mannose. Excess fructose inhibited the enzyme at the optimum pH (8.2) but not at pH 6.6. MgATP was inhibitory at pH 6.6. The apparent Michaelis-Menten constants at pH 8.2 were 0.057 mm for fructose and 0.10 mm for MgATP. Mg(2+) ions were essential for activity; Mn(2+) could partially replace Mg(2+). Fructokinase (fraction IV) had a requirement for K(+) ions which could be substantially replaced by Rb(+) or NH(4) (+) but not by Na(+). The enzyme was inhibited by MgADP. The possible significance of fructokinase (fraction IV) in plant carbohydrate metabolism is discussed.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Turner JF,Harrison DD,Copeland L

doi

10.1104/pp.60.5.666

subject

Has Abstract

pub_date

1977-11-01 00:00:00

pages

666-9

issue

5

eissn

0032-0889

issn

1532-2548

journal_volume

60

pub_type

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