Localization, solubilization and characterization of plant membrane-associated calcium-dependent protein kinases.

abstract：:The net initial passive flux (J(Ni)) in reconstituted plasma membrane (PM) vesicles from maize (Zea mays) root cells was measured as recently described (P. Pouliquin, J.-P. Grouzis, R. Gibrat ¿1999 Biophys J 76: 360-373). J(Ni) in control liposomes responded to membrane potential or to NO(3)(-) as expected from the Go...

journal_title：Plant physiology

authors： Pouliquin P,Boyer JC,Grouzis JP,Gibrat R

更新日期：2000-01-01 00:00:00

abstract：:Cell walls of grasses have two major polysaccharides that contain uronic acids, the hemicellulosic glucuronoarabinoxylans and the galactosyluronic acid-rich pectins. A technique whereby esterified uronic acid carboxyl groups are reduced selectively to yield their respective 6,6-dideuterio neutral sugars was used to de...

journal_title：Plant physiology

authors： Kim JB,Carpita NC

更新日期：1992-02-01 00:00:00

abstract：:The essential oils of peppermint (Mentha x piperita) and spearmint (Mentha spicata) are distinguished by the oxygenation position on the p-menthane ring of the constitutive monoterpenes that is conferred by two regiospecific cytochrome P450 limonene-3- and limonene-6-hydroxylases. Following hydroxylation of limonene, ...

journal_title：Plant physiology

authors： Ringer KL,Davis EM,Croteau R

更新日期：2005-03-01 00:00:00

abstract：:Dimethylallyl diphosphate (DMADP) and geranyl diphosphate (GDP) are the last precursors of isoprene and monoterpenes emitted by leaves, respectively. DMADP and GDP pools were measured in leaves of plants emitting isoprene (Populus alba), monoterpenes (Quercus ilex and Mentha piperita), or nonemitting isoprenoids (Prun...

journal_title：Plant physiology

authors： Nogués I,Brilli F,Loreto F

更新日期：2006-06-01 00:00:00

abstract：:The petunia (Petunia [Mitchell]) chloroplast proteins, the chlorophyll a/b-binding (Cab) proteins, and the small subunit of ribulose bisphosphate carboxylase (RbcS) are encoded by nuclear genes that are expressed in a light-dependent manner. The steady-state concentrations of five cab mRNAs vary with a dramatic circad...

journal_title：Plant physiology

authors： Stayton MM,Brosio P,Dunsmuir P

更新日期：1989-03-01 00:00:00

abstract：:The levels and types of phospholipids changed in distinct phases during the germination of (32)P-labeled uredospores of Uromyces phaseoli. During the first 20 minutes of germination, the phospholipid content dropped to 40% of the pregermination level. Between 2 and 3 hours, phospholipid levels increased to approximate...

journal_title：Plant physiology

authors： Langenbach RJ,Knoche HW

更新日期：1971-12-01 00:00:00

abstract：:Freezing, dehydration, and supercooling cause microtubules in mesophyll cells of spinach (Spinacia oleracea L. cv Bloomsdale) to depolymerize (ME Bartolo, JV Carter [1991] Plant Physiol 97: 175-181). The objective of this study was to gain insight into the question of whether microtubules depolymerize as a direct resp...

journal_title：Plant physiology

authors： Bartolo ME,Carter JV

更新日期：1992-08-01 00:00:00

abstract：:Pretreatment with pertussis toxin or microinjection of guanosine- 5[prime]-(3-thiotriphosphate) (GTP-[gamma]-S) into guard cells in peeled epidermis of Commelina communis L. promoted stomatal opening under subsaturating white light. Guanosine-5[prime]-(2-thiodiphosphate) (GDP-[beta]-S) and adenosine-5[prime]-(3-thiotr...

journal_title：Plant physiology

authors： Lee HJ,Tucker EB,Crain RC,Lee Y

更新日期：1993-05-01 00:00:00

abstract：:Growth of Zea mays L. cv Potro roots was inhibited by the herbicide metsulfuron methyl (MSM) at the lowest concentration tested: 5 nanomoles per liter. Pretreatment of corn seeds with commercial 1,8-naphthalic anhydride (NA) at 1% (w/w) partially reversed MSM-induced root growth inhibition. MSM at a concentration of 5...

journal_title：Plant physiology

authors： Milhomme H,Bastide J

更新日期：1990-06-01 00:00:00

abstract：:Stimulation of active oxygen metabolism occurs during the early stages of interactions involving bacteria and plant cell suspensions. Although many cellular processes are known to affect active oxygen metabolism in plants, it is not known which of these factors affect active oxygen levels during plant-bacteria interac...

journal_title：Plant physiology

authors： Baker CJ,Harmon GL,Glazener JA,Orlandi EW

更新日期：1995-05-01 00:00:00

abstract：:Partially purified aminoacyl synthetase preparations from pea roots (Pisum sativum L. var. Alaska) contain a heat-labile factor which can degrade leucyl-tRNA(6) (leu) to a new species. The singular electrophoretic and chromatographic mobilities, the isoprenoid nucleoside content, and the charging characteristics of th...

journal_title：Plant physiology

authors： Babcock DF,Morris RO

更新日期：1973-09-01 00:00:00

abstract：:We describe the mechanical, electronic, hydraulic, and structural design of a nonrecirculating hydroponic system. The system is particularly suited to studies at very low nutrient concentrations, for which on-line concentration monitoring methods either do not exist or are costly and limited to monitoring relatively f...

journal_title：Plant physiology

authors： Gutschick VP,Kay LE

更新日期：1991-04-01 00:00:00

abstract：:Stomatal movement, which regulates gas exchange in plants, is controlled by a variety of environmental factors, including biotic and abiotic stresses. The stress hormone abscisic acid (ABA) initiates a signaling cascade, which leads to increased H2O2 and Ca2+ levels and F-actin reorganization, but the mechanism of, an...

journal_title：Plant physiology

authors： Biel A,Moser M,Meier I

更新日期：2020-02-01 00:00:00

abstract：:AtATM3, an ATP-binding cassette transporter of Arabidopsis (Arabidopsis thaliana), is a mitochondrial protein involved in the biogenesis of iron-sulfur clusters and iron homeostasis in plants. Our gene expression analysis showed that AtATM3 is up-regulated in roots of plants treated with cadmium [Cd(II)] or lead (II);...

journal_title：Plant physiology

authors： Kim DY,Bovet L,Kushnir S,Noh EW,Martinoia E,Lee Y

更新日期：2006-03-01 00:00:00

abstract：:Crude aqueous extracts from Arabidopsis leaves were subjected to chromatographic separations, after which the different fractions were monitored for antimicrobial activity using the fungus Neurospora crassa as a test organism. Two major fractions were obtained that appeared to have the same abundance in leaves from un...

journal_title：Plant physiology

authors： Tierens KF,Thomma BP,Brouwer M,Schmidt J,Kistner K,Porzel A,Mauch-Mani B,Cammue BP,Broekaert WF

更新日期：2001-04-01 00:00:00

abstract：:A method has been developed to measure concentrations of CO(2) in gases rapidly. A gas sample is injected into a flowing carrier gas that passes through an infrared CO(2) analyzer. A strip chart recorded peak response is obtained which is proportional to the CO(2) concentration. A resolution of better than 2 microlite...

journal_title：Plant physiology

authors： Clegg MD,Sullivan CY,Eastin JD

更新日期：1978-12-01 00:00:00

abstract：:The flavoprotein (R)-(+)-mandelonitrile lyase (MDL; EC 4.1.2.10) plays a key role in cyanogenesis in rosaceous stone fruits. An MDL gene (mdl3) and its corresponding cDNA (MDL3) were isolated from black cherry (Prunus serotina) and characterized. The mdl3 gene contains 2292 bp of the 5' flanking region, the entire cod...

journal_title：Plant physiology

authors： Hu Z,Poulton JE

更新日期：1997-12-01 00:00:00

abstract：:In an earlier paper we showed that in fully developed barley (Hordeum vulgare L.) root epidermal cells a decrease in cytosolic K+ was associated with an acidification of the cytosol (D.J. Walker, R. A. Leigh, A.J. Miller [1996] Proc Natl Acad Sci USA 93: 10510-10514). To show that these changes in cytosolic ion concen...

journal_title：Plant physiology

authors： Walker DJ,Black CR,Miller AJ

更新日期：1998-11-01 00:00:00

abstract：:Wheat (Triticum aestivum L. cv Etoile de Choisy) microsomes catalyzed the cytochrome P-450-dependent oxidation of the herbicide diclofop to three hydroxy-diclofop isomers. Hydroxylation was predominant at carbon 4, with migration of chlorine to carbon 5 (67%) and carbon 3 (25%). The 2,4-dichloro-5-hydroxy isomer was i...

journal_title：Plant physiology

authors： Zimmerlin A,Durst F

更新日期：1992-10-01 00:00:00

abstract：:Simultaneous comparisons were made between a freezing-tolerant, cold-acclimating (CA) wild potato species (Solanum commersonii) and a freezing-sensitive, nonacclimating (NA) cultivated species (Solanum tuberosum). Comparative studies allowed differentiation of plasma membrane lipid changes associated with increased fr...

journal_title：Plant physiology

authors： Palta JP,Whitaker BD,Weiss LS

更新日期：1993-11-01 00:00:00

abstract：:Growth of the aerial part of the plant is dependent upon the maintenance of the shoot apical meristem (SAM). A balance between the self-renewing stem cells in the central zone (CZ) and organogenesis in the peripheral zone (PZ) is essential for the integrity, function, and maintenance of the SAM. Understanding how the ...

journal_title：Plant physiology

authors： Ung N,Lal S,Smith HM

更新日期：2011-06-01 00:00:00

abstract：:Symptoms typical of senescence occurred in green detached primary barley (Hordeum vulgare L.) leaves placed in darkness and in light. Chlorophyll, total soluble protein, ribulose 1,5-diphosphate carboxylase protein and activity each progressively decreased in darkness and to a lesser extent in light. In all treatments...

journal_title：Plant physiology

authors： Peterson LW,Huffaker RC

更新日期：1975-06-01 00:00:00

abstract：:Intact spheroplasts of the cyanobacterium (blue-green alga) Anacystis nidulans oxidized various exogenous c-type cytochromes with concomitant outward proton translocation while exogenous ferricytochrome c was not reduced. The H(+)/e(-) stoichiometry was close to 1 with each of the cytochromes and did not depend on the...

journal_title：Plant physiology

authors： Peschek GA

更新日期：1984-08-01 00:00:00

abstract：:Glutamine synthetase (GS) from Synechocystis sp. PCC 6803 was inactivated in vivo by transferring cells from light to darkness or by incubation with the photosynthetic inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea but not with 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone. Addition of glucose prevented both dark...

journal_title：Plant physiology

authors： Reyes JC,Crespo JL,Garcia-Dominguez M,Florencio FJ

更新日期：1995-11-01 00:00:00

abstract：:Sections of sugar beet (Beta vulgaris L.) taproot were incubated in various concentrations of mannitol. At 0.4, 0.6, and 0.8 molar, the membrane electrical potential difference (E(m)) averaged about -130 millivolts; at 0.2 molar, about -90 millivolts; and at 0 molar, between -60 and -80 millivolts. Additions of 10 mil...

journal_title：Plant physiology

authors： Kinraide TB,Wyse RE

更新日期：1986-12-01 00:00:00

abstract：:Single-cell RNA sequencing (scRNA-seq) has been used extensively to study cell-specific gene expression in animals, but it has not been widely applied to plants. Here, we describe the use of a commercially available droplet-based microfluidics platform for high-throughput scRNA-seq to obtain single-cell transcriptomes...

journal_title：Plant physiology

authors： Ryu KH,Huang L,Kang HM,Schiefelbein J

更新日期：2019-04-01 00:00:00

abstract：:Regulation of cortical microtubule reorganization is essential for plant cell survival under high salinity conditions. In response to salt stress, microtubules undergo rapid depolymerization followed by reassembly to form a new microtubule network that promotes cell survival; however, the upstream regulatory mechanism...

journal_title：Plant physiology

authors： Dou L,He K,Higaki T,Wang X,Mao T

更新日期：2018-03-01 00:00:00

abstract：:Indoleacetic acid oxidase in tobacco callus tissues (Nicotiana tabacum L., cultivar White Gold) was resolved into seven anionic isoenzymes by polyacrylamide gel disc electrophoresis. Different concentrations of kinetin and zeatin in the presence of indoleacetic acid affected the level of this enzyme, particularly two ...

journal_title：Plant physiology

authors： Lee TT

更新日期：1971-02-01 00:00:00

abstract：:ZRP4, a 1.4-kb mRNA that preferentially accumulates in roots of young Zea mays L. plants, was identified by isolation of the corresponding cDNA clone. Genomic Southern analysis indicates that the zrp4 gene is represented once in the corn genome. The deduced ZRP4 polypeptide of 39,558 D is rich in leucine, serine, and ...

journal_title：Plant physiology

authors： Held BM,Wang H,John I,Wurtele ES,Colbert JT

更新日期：1993-07-01 00:00:00

abstract：:Collection of allelopathic chemicals from the undisturbed plant root system is difficult because of their low concentrations and the high level of contaminants in growth media such as soil. A new approach for the continuous trapping of quantities of extracellular chemicals from donor plants is described. Bigalta limpo...

journal_title：Plant physiology

authors： Tang CS,Young CC

更新日期：1982-01-01 00:00:00