Nonrecirculating hydroponic system suitable for uptake studies at very low nutrient concentrations.

Abstract:

:We describe the mechanical, electronic, hydraulic, and structural design of a nonrecirculating hydroponic system. The system is particularly suited to studies at very low nutrient concentrations, for which on-line concentration monitoring methods either do not exist or are costly and limited to monitoring relatively few individual plants. Solutions are mixed automatically to chosen concentrations, which can be set differently for every pump fed from a master supply of deionized water and nutrient concentrates. Pumping rates can be varied over a 50-fold range, up to 400 liters per day, which suffices to maintain a number of large, post-seedling plants in rapid growth at (sub)micromolar levels of N and P. The outflow of each pump is divided among as many as 12 separate root chambers. In each chamber one may monitor uptake by individual plant roots or segments thereof, by measuring nutrient depletion in batch samples of solution. The system is constructed from nontoxic materials that do not adsorb nutrient ions; no transient shifts of nitrate and phosphate concentrations are observable at the submicromolar level. Nonrecirculation of solutions limits problems of pH shifts, microbial contamination, and cumulative imbalances in unmonitored nutrients. We note several disadvantages, principally related to high consumption of deionized water and solutes. The reciprocating pumps can be constructed inexpensively, particularly by the researcher. We also report previously unattainable control of passive temperature rise of chambers exposed to full sunlight, by use of white epoxy paint.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Gutschick VP,Kay LE

doi

10.1104/pp.95.4.1125

subject

Has Abstract

pub_date

1991-04-01 00:00:00

pages

1125-30

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

95

pub_type

杂志文章
  • Distribution of Gibberellins in Lathyrus odoratus L. and Their Role in Leaf Growth.

    abstract::In sweet pea (Lathyrus odoratus L.) the mutant allele l reduced the level of gibberellin A1 (GA1) in expanding leaflets and resulted in smaller, more oval leaflets compared with the wild type. The apical portions of 6-d-old wild-type (L) seedlings also contained less GA1 and produced smaller, more oval leaflets than d...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.2.603

    authors: Ross JJ,Murfet IC,Reid JB

    更新日期:1993-06-01 00:00:00

  • Induction of Inorganic Carbon Accumulation in the Unicellular Green Algae Scenedesmus obliquus and Chlamydomonas reinhardtii.

    abstract::The induction of a dissolved inorganic carbon (DIC) accumulating mechanism in the two algal species Scenedesmus obliquus (WT) and Chlamydomonas reinhardtii (137 c+) was physiologically characterized by monitoring DIC uptake kinetics at a low and constant DIC concentration (120-140 micromolar), after transfer from high...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.2.437

    authors: Palmqvist K,Sjöberg S,Samuelsson G

    更新日期:1988-06-01 00:00:00

  • Effect of heat shock on the metabolism of glutathione in maize roots.

    abstract::High performance liquid chromatography analyses revealed that glutathione (GSH) and cysteine are two of the major low molecular weight thiol compounds in maize root extracts. Treatment of maize roots to heat shock temperatures of 40 degrees C resulted in a decrease of cysteine levels and an increase of GSH levels. Pul...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.4.1031

    authors: Nieto-Sotelo J,Ho TH

    更新日期:1986-12-01 00:00:00

  • Sodium Requirement for Photosynthesis and Its Relationship with Dinitrogen Fixation and the External CO(2) Concentration in Cyanobacteria.

    abstract::Cells of Anabaena PCC 7119 and of a mutant strain of Nostoc muscorum unable to fix dinitrogen, grown at pH 8 and under low CO(2) tension (air), showed a reduced capacity for photosynthesis when cultured in the absence of sodium, this inhibition being followed by symptoms of photooxidation, such as chlorosis, oxygen co...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.2.585

    authors: Maeso ES,Piñas FF,Gonzalez MG,Valiente EF

    更新日期:1987-10-01 00:00:00

  • Evidence for the Contribution of the Mehler-Peroxidase Reaction in Dissipating Excess Electrons in Drought-Stressed Wheat.

    abstract::Gross O2 evolution and uptake by attached, drought-stressed leaves of wheat (Triticum aestivum) were measured using a 16O2/ 18O2 isotope technique and mass spectrometry. The activity of photosystem II, determined from the rate of 16O2 evolution, is only slightly affected under drought conditions. During drought stress...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.1.265

    authors: Biehler K,Fock H

    更新日期:1996-09-01 00:00:00

  • RiceArrayNet: a database for correlating gene expression from transcriptome profiling, and its application to the analysis of coexpressed genes in rice.

    abstract::Microarray data can be used to derive understanding of the relationships between the genes involved in various biological systems of an organism, given the availability of databases of gene expression measurements from the complete spectrum of experimental conditions and materials. However, there have been no reports,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.139030

    authors: Lee TH,Kim YK,Pham TT,Song SI,Kim JK,Kang KY,An G,Jung KH,Galbraith DW,Kim M,Yoon UH,Nahm BH

    更新日期:2009-09-01 00:00:00

  • Requirement for the H phosphoprotein in photosystem II of Chlamydomonas reinhardtii.

    abstract::To dissect the expression of the psbB gene cluster of the Chlamydomonas reinhardtii chloroplast genome and to assess the role of the photosystem II H-phosphoprotein (PSII-H) in the biogenesis and/or stabilization of PSII, an aadA gene cassette conferring spectinomycin resistance was employed for mutagenesis. Disruptio...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.4.1359

    authors: Summer EJ,Schmid VH,Bruns BU,Schmidt GW

    更新日期:1997-04-01 00:00:00

  • mGWAS Uncovers Gln-Glucosinolate Seed-Specific Interaction and its Role in Metabolic Homeostasis.

    abstract::Gln is a key player in plant metabolism. It is one of the major free amino acids that is transported into the developing seed and is central for nitrogen metabolism. However, Gln natural variation and its regulation and interaction with other metabolic processes in seeds remain poorly understood. To investigate the la...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00039

    authors: Slaten ML,Yobi A,Bagaza C,Chan YO,Shrestha V,Holden S,Katz E,Kanstrup C,Lipka AE,Kliebenstein DJ,Nour-Eldin HH,Angelovici R

    更新日期:2020-06-01 00:00:00

  • Abnormal Stomatal Behavior and Hormonal Imbalance in flacca, a Wilty Mutant of Tomato: IV. Effect of Abscisic Acid and Water Content on RNase Activity and RNA.

    abstract::Plants of the wilty tomato (Lycopersicum esculentum) mutant, flacca, and of the normal cultivar Rheinlands Ruhm growing under either "normal" or high humidity were used in this research. Under normal humidity, RNase activity was much higher in mutant plants in which abscisic acid (ABA) and water content were lower tha...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.2.173

    authors: Puri J,Tal M

    更新日期:1977-02-01 00:00:00

  • Bisphosphonate inhibitors reveal a large elasticity of plastidic isoprenoid synthesis pathway in isoprene-emitting hybrid aspen.

    abstract::Recently, a feedback inhibition of the chloroplastic 1-deoxy-D-xylulose 5-phosphate (DXP)/2-C-methyl-D-erythritol 4-phosphate (MEP) pathway of isoprenoid synthesis by end products dimethylallyl diphosphate (DMADP) and isopentenyl diphosphate (IDP) was postulated, but the extent to which DMADP and IDP can build up is n...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00470

    authors: Rasulov B,Talts E,Kännaste A,Niinemets Ü

    更新日期:2015-06-01 00:00:00

  • Production of volatile amines and skatole at anthesis in some arum lily species.

    abstract::The odoriferous compounds produced at the time of flowering by the inflorescences of 5 arum lily species were condensed in dry ice traps. The components of the condensates were separated by paper chromatography and identified on the basis of position and color reactions in comparison with known compounds. The concentr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.2.343

    authors: Smith BN,Meeuse BJ

    更新日期:1966-02-01 00:00:00

  • Lipids of plasma membranes prepared from oat root cells : effects of induced water-deficit tolerance.

    abstract::Plasma membranes were isolated from oat (Avena sativa) roots by the phase-partitioning method. The membranes were exposed to repeated periods of moderate water-deficit stress, and a water-deficit tolerance was induced (acclimated plants). The plasma membranes of the controls (nonacclimated plants) were characterized b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.4.1136

    authors: Norberg P,Liljenberg C

    更新日期:1991-08-01 00:00:00

  • Structure-activity relationship in the auxin activity of mono-substituted phenylacetic acids.

    abstract::The analysis of substituent constants for the lipophilic and electronic factors in the auxin activity of substituted phenylacetic acids in elongation of coleoptile segments shows that these factors parallel those for the phenoxyacetic acids but assign reactivity in growth promotion to the meta position of phenylacetic...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.11.1519

    authors: Muir RM,Fujita T,Hansch C

    更新日期:1967-11-01 00:00:00

  • Mutations in the TIR1 auxin receptor that increase affinity for auxin/indole-3-acetic acid proteins result in auxin hypersensitivity.

    abstract::The phytohormone auxin regulates virtually every aspect of plant development. The hormone directly mediates the interaction between the two members of the auxin coreceptor complex, a TRANSPORT INHIBITOR RESPONSE (TIR1)/AUXIN SIGNALING F-BOX protein and an AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) transcriptional repressor....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.215582

    authors: Yu H,Moss BL,Jang SS,Prigge M,Klavins E,Nemhauser JL,Estelle M

    更新日期:2013-05-01 00:00:00

  • The effects of drought stress on respiration of isolated corn mitochondria.

    abstract::Mitochondria were isolated from etiolated corn shoots (Zea mays L.) that were stressed to a measured water potential. The rates of mitochondrial respiration in state III, state IV, and without phosphate or ADP on a milligram protein basis decreased as water stress increased with succinate, malatepyruvate, or reduced n...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.48.4.413

    authors: Bell DT,Koeppe DE,Miller RJ

    更新日期:1971-10-01 00:00:00

  • The Plastid Lipase PLIP1 Is Critical for Seed Viability in diacylglycerol acyltransferase1 Mutant Seed.

    abstract::In developing Arabidopsis (Arabidopsis thaliana) seeds, the synthesis of triacylglycerol (TAG) is mediated primarily by the acyl-CoA-dependent enzyme diacylglycerol acyltransferase1 (DGAT1). In the absence of DGAT1 activity, phospholipid:diacylglycerol acyltransferase (PDAT1) plays an important role in TAG synthesis, ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00600

    authors: Aulakh K,Durrett TP

    更新日期:2019-08-01 00:00:00

  • Powdery mildew-induced Mla mRNAs are alternatively spliced and contain multiple upstream open reading frames.

    abstract::In barley (Hordeum vulgare), the Mla13 powdery mildew resistance gene confers Rar1-dependent, AvrMla13-specific resistance to Blumeria graminis f. sp. hordei (Bgh). We have identified cDNA and genomic copies of Mla13 and used this coiled-coil nucleotide-binding site leucine-rich repeat protein-encoding gene as a model...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.014407

    authors: Halterman DA,Wei F,Wise RP

    更新日期:2003-02-01 00:00:00

  • Blue Light Interference in the Phytochrome-controlled Germination of the Spores of Cheilanthes farinosa.

    abstract::Short exposure of the spores of Cheilanthes farinosa to low intensity red light promotes their germination, which is not reversed by a subsequent exposure to far red light. Germination is, however, inhibited by blue light administered before or after red light. Inhibition of germination by blue light is annulled by ex...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.51.2.306

    authors: Raghavan V

    更新日期:1973-02-01 00:00:00

  • Cu2+ Reduction by Tomato Root Plasma Membrane Vesicles.

    abstract::Reduction of Cu2+ by plasma membrane vesicles isolated from tomato (Lycopersicon esculentum Mill.) roots was investigated. Plants were grown in hydroponic culture with complete nutrition for 4 weeks or were deprived of Fe for the last 7 d. Plasma membrane vesicles were prepared by aqueous two-phase partitioning. Reduc...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.3.1093

    authors: Holden MJ,Crimmins TJ Jr,Chaney RL

    更新日期:1995-07-01 00:00:00

  • Attenuation of the Phenotype Caused by the Root-Inducing, Left-Hand, Transferred DNA and Its rolA Gene (Correlations with Changes in Polyamine Metabolism and DNA Methylation).

    abstract::We present four examples of attenuation of the transformed phenotype caused by the root-inducing, left-hand, transferred DNA from Agrobacterium rhizogenes in tobacco (Nicotiana tabacum). The first was associated with a genetic variable (homozygosity for the T-DNA), and the second was induced at the physiological level...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.1.259

    authors: Martin-Tanguy J,Sun LY,Burtin D,Vernoy R,Rossin N,Tepfer D

    更新日期:1996-05-01 00:00:00

  • Variations in leaf soluble amino acids and ammonium content in subtropical seagrasses related to salinity stress.

    abstract::A survey of leaf soluble amino acids was conducted for four subtropical seagrasses grown at several salinities. Proline functioned as an organic osmoticum in Halodule wrightii Aschers., Thallasia testudinum Banks ex Koenig, and Ruppia maritima L., while alanine functioned in an osmoregulatory capacity in Halophila eng...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.1.283

    authors: Pulich WM

    更新日期:1986-01-01 00:00:00

  • A circuit analog model for studying quantitative water relations of plant tissues.

    abstract::Using arrays of resistors and capacitors, a lumped circuit analog of plant tissue is developed. The circuit elements of the analog are identified in terms of physiological variables (hydraulic conductivities, water capacities, and cell dimensions) which can be measured in the laboratory. With the aid of a circuit simu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.64.5.712

    authors: Molz FJ,Kerns DV,Peterson CM,Dane JH

    更新日期:1979-11-01 00:00:00

  • The small GTPase ROP6 interacts with NFR5 and is involved in nodule formation in Lotus japonicus.

    abstract::Nod Factor Receptor5 (NFR5) is an atypical receptor-like kinase, having no activation loop in the protein kinase domain. It forms a heterodimer with NFR1 and is required for the early plant responses to Rhizobium infection. A Rho-like small GTPase from Lotus japonicus was identified as an NFR5-interacting protein. The...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.197269

    authors: Ke D,Fang Q,Chen C,Zhu H,Chen T,Chang X,Yuan S,Kang H,Ma L,Hong Z,Zhang Z

    更新日期:2012-05-01 00:00:00

  • The requirement for sodium as a micronutrient by species having the c(4) dicarboxylic photosynthetic pathway.

    abstract::Six species having characteristics of plants with the C(4) dicarboxylic photosynthetic pathway, Echinochloa utilis L. Ohwi et Yabuno (Japanese millet), Cynodon dactylon L. (Bermuda grass), Kyllinga brevifolia Rottb., Amaranthus tricolor L. cv. Early splendour, Kochia childsii Hort., and Portulaca grandiflora Hook (ros...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.5.794

    authors: Brownell PF,Crossland CJ

    更新日期:1972-05-01 00:00:00

  • Loss of Microtubules in the Interphase Cells of Onion (Allium cepa L.) Root Tips from the Cell Cortex and Their Appearance in the Cytoplasm after Treatment with Cycloheximide.

    abstract::As part of a project to investigate the mechanism of cortical microtubule (MT) alignment, we examined the effects of cycloheximide (CHM) on cortical MTs in the root tip cells of Allium cepa L. Results show that although a preprophase band of MTs remained in the cell cortex, interphase MTs disappeared from the cortical...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.1.281

    authors: Mineyuki Y,Iida H,Anraku Y

    更新日期:1994-01-01 00:00:00

  • Ca transport in membrane vesicles from pinto bean leaves and its alteration after ozone exposure.

    abstract::The influence of ozone on Ca(2+) transport in plant membranes from pinto bean (Phaseolus vulgaris L. var Pinto) leaves was investigated in vitro by means of a filtration method using purified vesicles. Two transport mechanisms located at the plasma membrane are involved in a response to ozone: (a) passive Ca(2+) influ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.2.788

    authors: Castillo FJ,Heath RL

    更新日期:1990-10-01 00:00:00

  • Abrupt increase in the level of hydrogen peroxide in leaves of winter wheat is caused by cold treatment.

    abstract::After cold treatment of seedlings of winter wheat (Triticum aestivum L.), levels of hydrogen peroxide in the leaves were measured. The concentration of hydrogen peroxide increased to about three times the control level within a few minutes, and returned to the normal level in 15 to 20 minutes. The elevated level of hy...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.97.3.1265

    authors: Okuda T,Matsuda Y,Yamanaka A,Sagisaka S

    更新日期:1991-11-01 00:00:00

  • Characterization of glyoxysomes from castor bean endosperm.

    abstract::Electron micrographs are presented which establish the identity of the components of the 3 major bands observed after sucrose density centrifugation of the crude particulate fraction from the endosperm of germinating castor bean seedlings. These are: mitochondria (density 1.19 g/cc), proplastids (density 1.23 g/cc) an...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.5.705

    authors: Breidenbach RW,Kahn A,Beevers H

    更新日期:1968-05-01 00:00:00

  • Arabidopsis actin-depolymerizing factor AtADF4 mediates defense signal transduction triggered by the Pseudomonas syringae effector AvrPphB.

    abstract::The actin cytoskeleton has been implicated in plant defenses against pathogenic fungi and oomycetes with limited, indirect evidence. To date, there are no reports linking actin with resistance against phytopathogenic bacteria. The dynamic behavior of actin filaments is regulated by a diverse array of actin-binding pro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.137604

    authors: Tian M,Chaudhry F,Ruzicka DR,Meagher RB,Staiger CJ,Day B

    更新日期:2009-06-01 00:00:00

  • Regulation of Glutamine Synthetase by Light and during Nitrogen Deficiency in Synchronous Chlorella sorokiniana.

    abstract::A method is described to achieve density labeling of proteins in unicellular algae by using (13)CO(2). This is a satisfactory procedure especially for work on nitrogen metabolism. The increase in activity of glutamine synthetase (EC 6.3.1.2.) and glutamate synthase (EC 1.4.7.1.) in Chlorella sorokiniana mediated by a ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.5.805

    authors: Tischner R

    更新日期:1980-11-01 00:00:00