Abstract:
:In developing Arabidopsis (Arabidopsis thaliana) seeds, the synthesis of triacylglycerol (TAG) is mediated primarily by the acyl-CoA-dependent enzyme diacylglycerol acyltransferase1 (DGAT1). In the absence of DGAT1 activity, phospholipid:diacylglycerol acyltransferase (PDAT1) plays an important role in TAG synthesis, consistent with the higher-than-expected oil content and altered fatty acid composition of dgat1 seed. Transcript profiling of developing wild type (Columbia-0) and dgat1-1 mutant seed identified 602 differentially expressed genes. Expression of genes important for the formation of phosphatidylcholine, including LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASE2, and REDUCED OLEATE DESATURATION1 were strongly upregulated, consistent with increased substrate supply for PDAT1. In addition, several genes lacking a defined role in TAG biosynthesis were also upregulated, including the α/β-hydrolase family gene PLIP1, which encodes a plastid-localized lipase. In most tissues, PLIP1 was expressed at equivalent levels in wild-type and dgat1 plants, except for developing seed, where transcript levels were higher in the dgat1 mutant. Seeds from plip1 mutant plants possessed a 20% reduction in oil content and were smaller than seed from wild-type plants. Crosses between dgat1 and plip1 failed to generate double-homozygous mutant plants. Reciprocal crossing with wild-type plants demonstrated that both male and female gametophytes could transmit the dgat1 plip1 double-mutant genotype. Double-homozygous dgat1 plip1 seed formed but was green and failed to germinate. The synthetic lethal phenotype of dgat1 with plip1 indicates an important role for PLIP1 in the absence of DGAT1 activity, likely by supplying polyunsaturated fatty acid substrates for PDAT1.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Aulakh K,Durrett TPdoi
10.1104/pp.19.00600subject
Has Abstractpub_date
2019-08-01 00:00:00pages
1962-1974issue
4eissn
0032-0889issn
1532-2548pii
pp.19.00600journal_volume
180pub_type
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