Chill-Induced Changes in the Activity and Abundance of the Vacuolar Proton-Pumping Pyrophosphatase from Mung Bean Hypocotyls.

Abstract:

:Changes in the properties of extractable vacuolar H+-pumping pyrophosphatase (V-PPase) and vacuolar ATPase activities in chilling-sensitive seedlings of mung bean (Vigna radiata) were investigated. Following chilling at 4[deg]C for 48 h, both hydrolytic and proton-pumping activities of the V-PPase increased 1.5- to 2-fold over controls and remained elevated even after 72 h at low temperatures. Vacuolar ATPase levels did not change significantly throughout the chilling regime. However a large increase in alcohol dehydrogenase activity during chilling suggests a shift toward fermentative metabolism, which can be expected to decrease ATPase activity in situ. Western blotting of vacuolar membrane-enriched fractions from control and treated plants has confirmed that the changes in V-PPase activity are mirrored by increases in the amount of pump protein. Results suggest a specific role for the V-PPase in protecting chill-sensitive plants from the injurious effects of low temperatures via the maintenance of the proton gradient across the vacuolar membrane.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Darley CP,Davies JM,Sanders D

doi

10.1104/pp.109.2.659

subject

Has Abstract

pub_date

1995-10-01 00:00:00

pages

659-665

issue

2

eissn

0032-0889

issn

1532-2548

pii

109/2/659

journal_volume

109

pub_type

杂志文章
  • Involvement of Abscisic Acid in Ethylene-Induced Cotyledon Abscission in Cotton Seedlings.

    abstract::Cotton (Gossypium hirsutum L. cv LG102) seedlings raised from seeds exposed to 100 [mu]M norflurazon (NFZ) during imbibition contained reduced levels of free abscisic acid (ABA) and were visibly achlorophyllous. Exposure of untreated cotton seedlings to ethylene concentrations >1 [mu]L/L for 24 h resulted in cotyledon...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.2.641

    authors: Suttle JC,Hultstrand JF

    更新日期:1993-02-01 00:00:00

  • Diurnal trends in net photosynthetic rate and carbohydrate levels of soybean leaves.

    abstract::A study was made of diurnal trends in net photosynthetic rate and carbohydrate levels of unifoliolate leaves of soybean (Glycine max L. Merrill) under constant environmental conditions (50,000-lux light intensity, 24.5 C air temperature, 60% relative humidity, and 300 microliters of CO(2) per liter of air).Net photosy...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.51.5.871

    authors: Upmeyer DJ,Koller HR

    更新日期:1973-05-01 00:00:00

  • Replication of DNA by nuclei isolated from soybean suspension cultures.

    abstract::DNA replication was studied in nuclei isolated from soybean cells grown in suspension culture. The isolation procedure involved the preparation of protoplasts, their lysis with a nonionic detergent and purification of nuclei. These nuclei synthesized low molecular weight DNA and joined these fragments into DNA of inte...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.4.726

    authors: Roman R

    更新日期:1980-10-01 00:00:00

  • AtKC1 and CIPK23 Synergistically Modulate AKT1-Mediated Low-Potassium Stress Responses in Arabidopsis.

    abstract::In Arabidopsis (Arabidopsis thaliana), the Shaker K(+) channel AKT1 conducts K(+) uptake in root cells, and its activity is regulated by CBL1/9-CIPK23 complexes as well as by the AtKC1 channel subunit. CIPK23 and AtKC1 are both involved in the AKT1-mediated low-K(+) (LK) response; however, the relationship between the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.01493

    authors: Wang XP,Chen LM,Liu WX,Shen LK,Wang FL,Zhou Y,Zhang Z,Wu WH,Wang Y

    更新日期:2016-04-01 00:00:00

  • Nitrogen recycling and remobilization are differentially controlled by leaf senescence and development stage in Arabidopsis under low nitrogen nutrition.

    abstract::Five recombinant inbred lines (RILs) of Arabidopsis (Arabidopsis thaliana), previously selected from the Bay-0 x Shahdara RIL population on the basis of differential leaf senescence phenotypes (from early senescing to late senescing) when cultivated under nitrogen (N)-limiting conditions, were analyzed to monitor meta...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.119040

    authors: Diaz C,Lemaître T,Christ A,Azzopardi M,Kato Y,Sato F,Morot-Gaudry JF,Le Dily F,Masclaux-Daubresse C

    更新日期:2008-07-01 00:00:00

  • The last step in cocaine biosynthesis is catalyzed by a BAHD acyltransferase.

    abstract::The esterification of methylecgonine (2-carbomethoxy-3β-tropine) with benzoic acid is the final step in the biosynthetic pathway leading to the production of cocaine in Erythoxylum coca. Here we report the identification of a member of the BAHD family of plant acyltransferases as cocaine synthase. The enzyme is capabl...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.248187

    authors: Schmidt GW,Jirschitzka J,Porta T,Reichelt M,Luck K,Torre JC,Dolke F,Varesio E,Hopfgartner G,Gershenzon J,D'Auria JC

    更新日期:2015-01-01 00:00:00

  • Purification and characterization of glutathione reductase isozymes specific for the state of cold hardiness of red spruce.

    abstract::Isozymes of glutathione reductase (GR) have been purified from red spruce (Picea rubens Sarg.) needles. Two isozymes could be separated by anion-exchange chromatography from both nonhardened or cold-hardened tissue. Based on chromatographic elution profiles, the isozymes were designated GR-1NH and GR-2NH in preparatio...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.1.205

    authors: Hausladen A,Alscher RG

    更新日期:1994-05-01 00:00:00

  • Simulation of Cl Uptake by Low-salt Barley Roots as a Test of Models of Salt Uptake.

    abstract::Computing techniques are used to simulate the course of uptake of K(+), Na(+), and Cl(-) by low-salt roots. Measurements of the fluxes of these ions in high-salt roots are used to calculate membrane permeabilities, which are then used to calculate cell uptake. In this way it is possible to test the predictive value of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.10.1417

    authors: Pitman MG

    更新日期:1969-10-01 00:00:00

  • Calcium-regulated proteolysis of eEF1A.

    abstract::Eukaryotic elongation factor 1alpha (eEF1A) can be post-translationally modified by the addition of phosphorylglycerylethanolamine (PGE). [(14)C]Ethanolamine was incorporated into the PGE modification, and with carrot (Daucus carota L.) suspension culture cells, eEF1A was the only protein that incorporated detectable ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.3.957

    authors: Ransom-Hodgkins WD,Brglez I,Wang X,Boss WF

    更新日期:2000-03-01 00:00:00

  • Selection and initial characterization of partially nitrate tolerant nodulation mutants of soybean.

    abstract::Since NO(3) (-) availability in the rooting medium seriously limits symbiotic N(2) fixation by soybean (Glycine max [L.] Merr.), studies were initiated to select nodulation mutants which were more tolerant to NO(3) (-) and were adapted to the Midwest area of the United States. Three independent mutants were selected i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.1.169

    authors: Gremaud MF,Harper JE

    更新日期:1989-01-01 00:00:00

  • Involvement of Calcium and Calmodulin in Membrane Deterioration during Senescence of Pea Foliage.

    abstract::The prospect that Ca(2+) promotes senescence by activating calmodulin has been examined using cut pea (Pisum sativum co Alaska) foliage as a model system. Senescence was induced by severing 17-day-old plants from their roots and maintaining them in aqueous test solutions in the dark for an additional 4 days. Treatment...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.2.329

    authors: Leshem YY,Sridhara S,Thompson JE

    更新日期:1984-06-01 00:00:00

  • Fractionation of the three stable oxygen isotopes by oxygen-producing and oxygen-consuming reactions in photosynthetic organisms.

    abstract::The triple isotope composition (delta17O and delta18O) of dissolved O2 in the ocean and in ice cores was recently used to assess the primary productivity over broad spatial and temporal scales. However, assessment of the productivity with the aid of this method must rely on accurate measurements of the 17O/16O versus ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.063768

    authors: Helman Y,Barkan E,Eisenstadt D,Luz B,Kaplan A

    更新日期:2005-08-01 00:00:00

  • Modulation of PSI and PSII Organization During Loss and Repair of Photosynthetic Activity in a Temperature Sensitive Mutant of Chlorella pyrenoidosa.

    abstract::Photosynthetic activity and organization of chlorophyll(Chl)-protein complexes in a temperature sensitive mutant of Chlorella pyrenoidosa have been investigated. The mutant is practically indistinguishable from wild type cells when grown at 25 C. However, mutant cells grown at 33 C do not synthesize Chl and lose their...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.6.1264

    authors: Lavintman N,Galling G,Ohad I

    更新日期:1981-12-01 00:00:00

  • BIG Regulates Dynamic Adjustment of Circadian Period in Arabidopsis thaliana.

    abstract::Circadian clocks drive rhythms with a period near 24 h, but the molecular basis of the regulation of the period of the circadian clockis poorly understood. We previously demonstrated that metabolites affect the free-running period of the circadian oscillator of Arabidopsis (Arabidopsis thaliana), with endogenous sugar...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.00571

    authors: Hearn TJ,Marti Ruiz MC,Abdul-Awal SM,Wimalasekera R,Stanton CR,Haydon MJ,Theodoulou FL,Hannah MA,Webb AAR

    更新日期:2018-09-01 00:00:00

  • Distribution of Gibberellins in Lathyrus odoratus L. and Their Role in Leaf Growth.

    abstract::In sweet pea (Lathyrus odoratus L.) the mutant allele l reduced the level of gibberellin A1 (GA1) in expanding leaflets and resulted in smaller, more oval leaflets compared with the wild type. The apical portions of 6-d-old wild-type (L) seedlings also contained less GA1 and produced smaller, more oval leaflets than d...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.2.603

    authors: Ross JJ,Murfet IC,Reid JB

    更新日期:1993-06-01 00:00:00

  • Promotion of testa rupture during garden cress germination involves seed compartment-specific expression and activity of pectin methylesterases.

    abstract::Pectin methylesterase (PME) controls the methylesterification status of pectins and thereby determines the biophysical properties of plant cell walls, which are important for tissue growth and weakening processes. We demonstrate here that tissue-specific and spatiotemporal alterations in cell wall pectin methylesterif...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.247429

    authors: Scheler C,Weitbrecht K,Pearce SP,Hampstead A,Büttner-Mainik A,Lee KJ,Voegele A,Oracz K,Dekkers BJ,Wang X,Wood AT,Bentsink L,King JR,Knox JP,Holdsworth MJ,Müller K,Leubner-Metzger G

    更新日期:2015-01-01 00:00:00

  • Mechanism of photoactivation of electron transport in intact bryopsis chloroplasts.

    abstract::The mechanism of photoactivation of photosystem I electron transport was studied in intact Bryopsis corticulans chloroplasts. The evidence from chemical and photochemical studies suggests that photoactivation is a consequence of a reduction of an electron transport component, presumably ferredoxin-NADP(+) reductase. O...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.5.1413

    authors: Satoh K

    更新日期:1982-11-01 00:00:00

  • A Proteinase from Germinating Barley : I. Purification and Some Physical Properties of a 30 kD Cysteine Endoproteinase from Green Malt.

    abstract::A proteinase was purified from germinated barley (green malt from Hordeum vulgare L. cv Morex) by acidic extraction, ammonium sulfate fractionation and successive chromatographies on CM-cellulose, hemoglobin sepharose, Sephadex G-75 and organomercurial agarose columns. The overall purification and final recovery were ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.4.1454

    authors: Poulle M,Jones BL

    更新日期:1988-12-01 00:00:00

  • Gene locus specificity of the glucose effect in the thiamine pathway of the angiosperm, Arabidopsis.

    abstract::All mutants at 3 loci in Arabidopsis thaliana (L.) Heynh., a higher plant, that are associated with the synthesis or coupling of the thiazole moiety of thiamine are susceptible to reversible glucose inhibition. In contrast, several different alleles involved in the synthesis of the pyrimidine moiety of the vitamin are...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.2.225

    authors: Li SL,Redei GP

    更新日期:1969-02-01 00:00:00

  • Phosphate Starvation Alters Abiotic-Stress-Induced Cytosolic Free Calcium Increases in Roots.

    abstract::Phosphate (Pi) deficiency strongly limits plant growth, and plant roots foraging the soil for nutrients need to adapt to optimize Pi uptake. Ca2+ is known to signal in root development and adaptation but has to be tightly controlled, as it is highly toxic to Pi metabolism. Under Pi starvation and the resulting decreas...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.01469

    authors: Matthus E,Wilkins KA,Swarbreck SM,Doddrell NH,Doccula FG,Costa A,Davies JM

    更新日期:2019-04-01 00:00:00

  • The Arabidopsis abscisic acid catabolic gene CYP707A2 plays a key role in nitrate control of seed dormancy.

    abstract::Nitrate releases seed dormancy in Arabidopsis (Arabidopsis thaliana) Columbia accession seeds in part by reducing abscisic acid (ABA) levels. Nitrate led to lower levels of ABA in imbibed seeds when included in the germination medium (exogenous nitrate). Nitrate also reduced ABA levels in dry seeds when provided to th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.126938

    authors: Matakiadis T,Alboresi A,Jikumaru Y,Tatematsu K,Pichon O,Renou JP,Kamiya Y,Nambara E,Truong HN

    更新日期:2009-02-01 00:00:00

  • Next-generation systemic acquired resistance.

    abstract::Systemic acquired resistance (SAR) is a plant immune response to pathogen attack. Recent evidence suggests that plant immunity involves regulation by chromatin remodeling and DNA methylation. We investigated whether SAR can be inherited epigenetically following disease pressure by Pseudomonas syringae pv tomato DC3000...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.187468

    authors: Luna E,Bruce TJ,Roberts MR,Flors V,Ton J

    更新日期:2012-02-01 00:00:00

  • Agrobacterium tumefaciens Site Attachment as a Necessary Prerequisite for Crown Gall Tumor Formation on Potato Discs.

    abstract::The infectivity of Agrobacterium tumefaciens strain B6 was inhibited about 50% when these bacteria were inoculated on potato discs with equal viable cell counts of a weakly virulent strain of A. tumefaciens (B-48) or autoclaved strains of B6 or B-48. Inhibition by B-48 or autoclaved B6 could still be obtained when the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.6.1031

    authors: Glogowski W,Galsky AG

    更新日期:1978-06-01 00:00:00

  • The procambium specification gene Oshox1 promotes polar auxin transport capacity and reduces its sensitivity toward inhibition.

    abstract::The auxin-inducible homeobox gene Oshox1 of rice (Oryza sativa) is a positive regulator of procambial cell fate commitment, and its overexpression reduces the sensitivity of polar auxin transport (PAT) to the PAT inhibitor 1-N-naphthylphthalamic acid (NPA). Here, we show that wild-type rice leaves formed under conditi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.009167

    authors: Scarpella E,Boot KJ,Rueb S,Meijer AH

    更新日期:2002-11-01 00:00:00

  • fhy3-1 retains inductive responses of phytochrome A.

    abstract::The fhy3 mutation of Arabidopsis impairs phytochrome A (phyA)-mediated inhibition of hypocotyl growth without affecting the levels of phyA measured spectrophotometrically or immunochemically. We investigated whether the fhy3-1 mutation has similar effects on very low fluence responses (VLFR) and high irradiance respon...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.123.1.235

    authors: Yanovsky MJ,Whitelam GC,Casal JJ

    更新日期:2000-05-01 00:00:00

  • Cell-specific photosynthetic gene expression in maize determined using cell separation techniques and hybridization in situ.

    abstract::Bundle sheath strands and mesophyll cell extracts have been isolated from maize (Zea mays L.) leaves using a mechanical disruption-filtration technique. Northern blot analysis showed that phosphoenolpyruvate carboxylase (PEPCase; EC 4.1.1.31) mRNA accumulates only in mesophyll cells. The mechanisms regulating the cell...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.2.613

    authors: Martineau B,Taylor WC

    更新日期:1986-10-01 00:00:00

  • On the Relationships of Net CO(2) Assimilation and Leaf Expansion to Vegetative Growth in Lycospersicum esculentum, var Jubilee.

    abstract::Relationships between net plant CO(2) exchange rate (CER) and canopy development were examined in ;jubilee' tomato over the initial 4 weeks of vegetative growth. A comparison was made between two plant groups that were alternatively exposed to 200 or 800 microeinsteins per square meter per second midday irradiation to...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.3.711

    authors: Campbell DE,Lyman M,Corse J,Hautala E

    更新日期:1986-03-01 00:00:00

  • On the uptake, metabolism and retention of [h] gibberellin a(1) by barley aleurone layers at low temperatures.

    abstract::Barley (c.v. Himalaya) aleurone layers were incubated in [(3)H]gibberellin A(1) (GA(1)) at low temperatures. At 3 and 4 C, (3)H-activity was steadily accumulated in aleurone layers, and this accumulation was correlated with significant [(3)H]GA(1) metabolism. At 1 and 1.5 C, metabolism could not be detected, and at th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.5.956

    authors: Keith B,Boal R,Srivastava LM

    更新日期:1980-11-01 00:00:00

  • Phosphorylation of the amino terminus of maize sucrose synthase in relation to membrane association and enzyme activity.

    abstract::Sucrose synthase (SUS) is phosphorylated on a major, amino-terminal site located at Ser-15 (S15) in the maize (Zea mays) SUS1 protein. Site- and phospho-specific antibodies against a phosphorylated S15 (pS15) peptide allowed direct analysis of S15 phosphorylation in relation to membrane association. Immunoblots of the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.036780

    authors: Hardin SC,Winter H,Huber SC

    更新日期:2004-04-01 00:00:00

  • Variable Chlorophyll a Fluorescence and CO(2) Uptake in Water-Stressed White Spruce Seedlings.

    abstract::Regulation of photosynthetic activity can contribute to the prevention of photodamage in stress resistant plants during exposure to drought or low temperatures. Responses to increasing levels of water stress were examined in seedlings of the stress resistant forest conifer, white spruce (Picea glauca [Moench.] Voss). ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.3.744

    authors: Toivonen P,Vidaver W

    更新日期:1988-03-01 00:00:00