Replication of DNA by nuclei isolated from soybean suspension cultures.

Abstract:

:DNA replication was studied in nuclei isolated from soybean cells grown in suspension culture. The isolation procedure involved the preparation of protoplasts, their lysis with a nonionic detergent and purification of nuclei. These nuclei synthesized low molecular weight DNA and joined these fragments into DNA of intermediate molecular weight. The characteristics of replication in isolated nuclei correlated well with those of the cells from which they were isolated, as shown by fluorodeoxyuridine synchronization and ultraviolet irradiation experiments.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Roman R

doi

10.1104/pp.66.4.726

subject

Has Abstract

pub_date

1980-10-01 00:00:00

pages

726-30

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

66

pub_type

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