Abstract:
:DNA replication was studied in nuclei isolated from soybean cells grown in suspension culture. The isolation procedure involved the preparation of protoplasts, their lysis with a nonionic detergent and purification of nuclei. These nuclei synthesized low molecular weight DNA and joined these fragments into DNA of intermediate molecular weight. The characteristics of replication in isolated nuclei correlated well with those of the cells from which they were isolated, as shown by fluorodeoxyuridine synchronization and ultraviolet irradiation experiments.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Roman Rdoi
10.1104/pp.66.4.726subject
Has Abstractpub_date
1980-10-01 00:00:00pages
726-30issue
4eissn
0032-0889issn
1532-2548journal_volume
66pub_type
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