Induction and Accumulation of Heat Shock-Specific Poly(A) RNAs and Proteins in Soybean Seedlings during Arsenite and Cadmium Treatments.

Abstract:

:Northern blot hybridization analyzes revealed that poly(A(+)) RNAs homologous to eight heat shock (HS)-specific cDNA clones were induced by arsenite (As) or Cd treatments. The mRNAs accumulated slower, and maximum accumulations were consistently lower than HS-induced levels. Prolonged treatment with low concentrations (50-100 micromolar) of As for 6 hours, or Cd for 12 hours, resulted in decreased accumulations of HS-specific mRNAs. This response resembled the ;autoregulation' observed during continuous 40 degrees C HS. However, no autoregulation was evident when soybean seedlings were exposed to high concentrations of As (250 micromolar) or Cd (1 millimolar) for 12 hours. The cDNA probe pCE54 detected a second higher molecular weight poly(A(+)) RNA following As or Cd treatments which accumulated concomitantly with the lower molecular weight HS-specific poly(A(+)) RNA. The patterns of low molecular weight HS polypeptides from in vitro translations induced by HS, As, and Cd, and analyzed by one-dimensional and two-dimensional SDS-PAGE, were similar but temporal differences were apparent. In addition to HS proteins, many control proteins were also detected in both in vitro and in vivo labeling patterns from As and, to a lesser extent, Cd treatments. The chemical agents used in this study apparently induced the accumulation and translation of HS messages in vivo but not in the selective manner as observed during HS treatment.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Edelman L,Czarnecka E,Key JL

doi

10.1104/pp.86.4.1048

subject

Has Abstract

pub_date

1988-04-01 00:00:00

pages

1048-56

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

86

pub_type

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