Abstract:
:Despite the importance of sulfur (S) for plant nutrition, the role of the arbuscular mycorrhizal (AM) symbiosis in S uptake has received little attention. To address this issue, 35S-labeling experiments were performed on mycorrhizas of transformed carrot (Daucus carota) roots and Glomus intraradices grown monoxenically on bicompartmental petri dishes. The uptake and transfer of 35SO4(2-) by the fungus and resulting 35S partitioning into different metabolic pools in the host roots was analyzed when altering the sulfate concentration available to roots and supplying the fungal compartment with cysteine (Cys), methionine (Met), or glutathione. Additionally, the uptake, transfer, and partitioning of 35S from the reduced S sources [35S]Cys and [35S]Met was determined. Sulfate was taken up by the fungus and transferred to mycorrhizal roots, increasing root S contents by 25% in a moderate (not growth-limiting) concentration of sulfate. High sulfate levels in the mycorrhizal root compartment halved the uptake of 35SO4(2-) from the fungal compartment. The addition of 1 mm Met, Cys, or glutathione to the fungal compartment reduced the transfer of sulfate by 26%, 45%, and 80%, respectively, over 1 month. Similar quantities of 35S were transferred to mycorrhizal roots whether 35SO4(2-), [35S]Cys, or [35S]Met was supplied in the fungal compartment. Fungal transcripts for putative S assimilatory genes were identified, indicating the presence of the trans-sulfuration pathway. The suppression of fungal sulfate transfer in the presence of Cys coincided with a reduction in putative sulfate permease and not sulfate adenylyltransferase transcripts, suggesting a role for fungal transcriptional regulation in S transfer to the host. A testable model is proposed describing root S acquisition through the AM symbiosis.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Allen JW,Shachar-Hill Ydoi
10.1104/pp.108.129866subject
Has Abstractpub_date
2009-01-01 00:00:00pages
549-60issue
1eissn
0032-0889issn
1532-2548pii
pp.108.129866journal_volume
149pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::The idea that embolized xylem vessels can be refilled while adjacent vessels remain under tension is difficult to accept if the cavitated vessels remain hydraulically connected to vessels under tension. A mechanism by which embolized conduits could be hydraulically isolated from adjacent conduits requires the existenc...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.123.3.1015
更新日期:2000-07-01 00:00:00
abstract::Studies were conducted with 9 to 12 day-old soybean (Glycine max [L.] Merr. cv. Williams) seedlings to determine the contribution of roots to whole plant NO(3) (-) reduction. Using an in vivo -NO(3) (-) nitrate reductase (NR) assay (no exogenous NO(3) (-) added to incubation medium) developed for roots, the roots acco...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.6.1298
更新日期:1982-06-01 00:00:00
abstract::Nod Factor Receptor5 (NFR5) is an atypical receptor-like kinase, having no activation loop in the protein kinase domain. It forms a heterodimer with NFR1 and is required for the early plant responses to Rhizobium infection. A Rho-like small GTPase from Lotus japonicus was identified as an NFR5-interacting protein. The...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.197269
更新日期:2012-05-01 00:00:00
abstract::The expression of alternative oxidase (Aox) and uncoupling proteins (Ucp) was investigated during ripening in mango (Mangifera indica) and compared with the expression of peroxisomal thiolase, a previously described ripening marker in mango. The multigene family for the Aox in mango was expressed differentially during...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.126.4.1619
更新日期:2001-08-01 00:00:00
abstract::We report here a method for the isolation of high specific activity phosphoglycerate kinase (EC 2.7.2.3) from chloroplasts. The enzyme has been purified over 200-fold from pea (Pisum sativum L.) stromal extracts to apparent homogeneity with 23% recovery. Negative cooperativity is observed with the two enzyme phosphogl...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.1.291
更新日期:1990-09-01 00:00:00
abstract::The light dependence of D1 phosphorylation is unique to higher plants, being constitutive in cyanobacteria and algae. In a photoautotrophic higher plant, Spirodela oligorrhiza, grown in greenhouse conditions under natural diurnal cycles of solar irradiation, the ratio of phosphorylated versus total D1 protein (D1-P in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.013441
更新日期:2002-12-01 00:00:00
abstract::The expression of CHALCONE SYNTHASE (CHS) expression is an important control step in the biosynthesis of flavonoids, which are major photoprotectants in plants. CHS transcription is regulated by endogenous programs and in response to environmental signals. Luciferase reporter gene fusions showed that the CHS promoter ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.005405
更新日期:2002-09-01 00:00:00
abstract::Bacterial blight is a devastating disease of rice (Oryza sativa) caused by Xanthomonas oryzae pv oryzae (Xoo). Zinc finger proteins harboring the motif with three conserved cysteine residues and one histidine residue (CCCH) belong to a large family. Although at least 67 CCCH-type zinc finger protein genes have been id...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.191379
更新日期:2012-02-01 00:00:00
abstract::Since peroxidase and manganese have been implicated in both auxin destruction and ethylene production, the effect of auxins and high tissue levels of manganese on the peroxidative indoleacetic acid oxidase system and the internal level of ethylene was determined in cotton (Gossypium hirsutum L. cv. Watson GL-7). The h...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.49.4.555
更新日期:1972-04-01 00:00:00
abstract::Plants of a single genotype of wild strawberry, Fragaria virginiana Duchesne, were grown with or without fertilizer in high (406 microeinsteins per square meter per second) and low (80 microeinsteins per square meter per second) light. High-light leaves were thicker than low-light leaves and had greater development of...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.4.1044
更新日期:1982-10-01 00:00:00
abstract::The rate of CO(2) reduction in the S-triazine-resistant biotype of smooth pigweed (Amaranthus hybridus L.) was lower at all levels of irradiance than the rate of CO(2) reduction in the susceptible biotype. The intent of this study was to determine whether or not the lower rates of CO(2) reduction are a direct conseque...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.72.4.925
更新日期:1983-08-01 00:00:00
abstract::Several cell wall-bound glycosidases present in Avena sativa coleoptiles were assayed by following the hydrolysis of p-nitrophenyl-glycosides. Particular emphasis was placed on characterizing some parameters affecting the activity of beta-galactosidase. The pH optimum of this enzyme is 4.5 to 5.5; it is sensitive to c...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.53.2.224
更新日期:1974-02-01 00:00:00
abstract::NADP-glyceraldehyde-3-P dehydrogenase of spinach (Spinacia oleracea) chloroplasts was activated by thioredoxin that was reduced either photochemically with ferredoxin and ferredoxin-thioredoxin reductase or chemically with dithiothreitol. The activation process that was observed with the soluble protein fraction from ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.61.4.669
更新日期:1978-04-01 00:00:00
abstract::Glycolate oxidase (GO) has been identified in the endocyanom Cyanophora paradoxa which has peroxisome-like organelles and cyanelles instead of chloroplasts. The enzyme used or formed equimolar amounts of O(2) or H(2)O(2) and glyoxylate, respectively. Aerobically, the enzyme did not reduce the artificial electron accep...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.3.887
更新日期:1992-03-01 00:00:00
abstract::This study presents evidence for a new enzyme, d-ribose-5-P reductase, which catalyzes the reaction: d-ribose-5-P + NADPH + H(+) --> d-ribitol-5-P + NADP(+). The enzyme was isolated from Adonis vernalis L. leaves in 38% yield and was purified 71-fold. The reductase was NADPH specific and had a pH optimum in the range ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.4.758
更新日期:1985-08-01 00:00:00
abstract::We investigated the function of cell wall hydroxyproline-rich glycoproteins by observing the effects of a selective inhibitor of prolyl hydroxylase, 3,4-dehydro-L-proline (Dhp), on wall regeneration by Nicotiana tabacum mesophyll cell protoplasts. Protoplasts treated with micromolar concentrations of Dhp do not develo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.2.747
更新日期:1994-02-01 00:00:00
abstract::Chymotrypsin inhibitor I is a protein that can be induced to accumulate in potato leaflets within hr in the light when leaflets are detached from intact plants and supplied with water. This increase in inhibitor I is not accompanied by an increase in all proteins in the detached leaflets. The accumulation is polar, pr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.11.1859
更新日期:1968-11-01 00:00:00
abstract::A number of early nodulin genes are expressed in specific cell types as pea (Pisum sativum) root nodules develop. The Pisum sativum early nodulin PsENOD2 is detected only in the uninfected cells of the nodule parenchyma, whereas PsENOD12 is expressed at two spatially removed sites: in root hairs and adjacent cortical ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.4.1649
更新日期:1992-12-01 00:00:00
abstract::Temperature-induced changes in the enzymes for fatty acid synthesis and desaturation were studied in developing soybean seeds (Glycine max L. var Williams 82). Changes were induced by culture of the seed pods for 20 hours in liquid media at 20, 25, or 35 degrees C. Linoleoyl and oleoyl desaturases were 94 and 10 times...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.2.760
更新日期:1989-06-01 00:00:00
abstract::Systemic acquired resistance (SAR) is a plant immune response to pathogen attack. Recent evidence suggests that plant immunity involves regulation by chromatin remodeling and DNA methylation. We investigated whether SAR can be inherited epigenetically following disease pressure by Pseudomonas syringae pv tomato DC3000...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.187468
更新日期:2012-02-01 00:00:00
abstract::The effect of the exogenous and endogenous NO(3) (-) concentration on net uptake, influx, and efflux of NO(3) (-) and on nitrate reductase activity (NRA) in roots was studied in Phaseolus vulgaris L. cv. Witte Krombek. After exposure to NO(3) (-), an apparent induction period of about 6 hours occurred regardless of th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.3.754
更新日期:1982-09-01 00:00:00
abstract::C-TERMINALLY ENCODED PEPTIDEs (CEPs) control root system architecture in a non-cell-autonomous manner. In Medicago truncatula, MtCEP1 affects root development by increasing nodule formation and inhibiting lateral root emergence by unknown pathways. Here, we show that the MtCEP1 peptide-dependent increase in nodulation...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00113
更新日期:2016-08-01 00:00:00
abstract::Kinematic analysis has provided important insights into the biology of growth by revealing the distribution of expansion within growing organs. Modern methods of kinematic analysis have made use of new image-tracking algorithms and computer-assisted evaluation, but these methods have yet to be adapted for examination ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.103226
更新日期:2007-10-01 00:00:00
abstract::Plants commonly live in a symbiotic association with arbuscular mycorrhizal fungi (AMF). They invest photosynthetic products to feed their fungal partners, which, in return, provide mineral nutrients foraged in the soil by their intricate hyphal networks. Intriguingly, AMF can link neighboring plants, forming common m...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.195727
更新日期:2012-06-01 00:00:00
abstract::Experiments were designed to obtain evidence about the possible role of xanthophylls as abscisic acid (ABA) precursors in water-stressed leaves of Phaseolus vularis L. Leaves were exposed to (14)CO(2) and the specific activities of several major leaf xanthophylls and stress-induced ABA were determined after a chase in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.4.910
更新日期:1987-12-01 00:00:00
abstract::Gibberellins (GAs) are involved in regulation of many aspects during plant development. To investigate the impact of altered GA levels on plant growth and metabolism, transgenic tobacco (Nicotiana tabacum) plants have been engineered to express either a GA20-oxidase (AtGA20-ox) or a GA2-oxidase (AtGA2-ox) gene from Ar...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.036988
更新日期:2004-05-01 00:00:00
abstract::Cytidine deoxyriboside (Cdr), uridine deoxyriboside (Udr), and guanosine deoxyriboside (Gdr), induce quantitative bleaching of the fronds of Lemna minor (duckweed) during growth in continuous light on photoheterotrophic medium. Cdr-induced bleaching is not accompanied by a reduction in frond multiplication rate, but U...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.61.6.989
更新日期:1978-06-01 00:00:00
abstract::The cultivated tomato (Lycopersicon esculentum) has a unipinnate compound leaf. In the developing leaf primordium, major leaflet initiation is basipetal, and lobe formation and early vascular differentiation are acropetal. We show that engineered alterations in the expression of a tomato homeobox gene, LeT6, can cause...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.117.3.771
更新日期:1998-07-01 00:00:00
abstract::Despite ongoing research on carotenoid biosynthesis in model organisms, there is a paucity of information on pathway regulation operating in the grasses (Poaceae), which include plants of world-wide agronomic importance. As a result, efforts to either breed for or metabolically engineer improvements in carotenoid cont...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.039818
更新日期:2004-07-01 00:00:00
abstract::A basic problem in silicon (Si) uptake studies in biology is the lack of an appropriate radioactive isotope. Radioactive germanium-68 ((68)Ge) has been used previously as a Si tracer in biological materials, but its suitability for the study of Si transport in higher plants is still untested. In this study, we investi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.090845
更新日期:2007-01-01 00:00:00