3,4-Dehydroproline inhibits cell wall assembly and cell division in tobacco protoplasts.

Abstract:

:We investigated the function of cell wall hydroxyproline-rich glycoproteins by observing the effects of a selective inhibitor of prolyl hydroxylase, 3,4-dehydro-L-proline (Dhp), on wall regeneration by Nicotiana tabacum mesophyll cell protoplasts. Protoplasts treated with micromolar concentrations of Dhp do not develop osmotic stability and do not initiate mitosis. The architecture of regenerated cell walls was examined using deep-etch, freeze-fracture electron microscopy of rapidly frozen tobacco cells. Untreated protoplasts assemble a dense fibrillar cell wall consisting of laterally associating subelementary fibrils. In contrast, treatment of protoplasts with Dhp alters the structure of the regenerated wall fibrils in several ways: first, the microfibrils are coated with globular knobs; second, some larger fiber bundles have an open ribbon-like appearance; and third, the smallest subelementary fibrils were not visible. Tobacco cells develop an abnormal morphology as a consequence of this abnormal cell wall structure. Thus, inhibition of prolyl hydroxylase results in the regeneration of a cell wall with abnormal structural and functional properties. These data provide experimental evidence that hydroxyproline-rich glycoproteins are important for the structural integrity of primary cell walls and for the correct assembly of other wall polymers, and that wall structure is an important regulator of cell division and cell morphology.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Cooper JB,Heuser JE,Varner JE

doi

10.1104/pp.104.2.747

subject

Has Abstract

pub_date

1994-02-01 00:00:00

pages

747-52

issue

2

eissn

0032-0889

issn

1532-2548

pii

104/2/747

journal_volume

104

pub_type

杂志文章
  • Evidence for a Block between Plastoquinone and Cytochrome f in a Photosynthetic Mutant of Lemna with Abnormal Flowering Behavior.

    abstract::Mutant strain 1073 of Lemna perpusilla is concluded to be blocked between plastoquinone and cytochrome f in the photosynthetic electron transport system. The location of the block is based on the following observations of activities in chloroplasts isolated from the mutant and wild-type plants. (a) Relative to wild ty...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.4.577

    authors: Shahak Y,Posner HB,Avron M

    更新日期:1976-04-01 00:00:00

  • Cytochemical localization of catalase activity in glyoxysomes from castor bean endosperm.

    abstract::Glyoxysomes isolated from castor bean (Ricinus communis L. var. zanzibariensis) endosperm have been stained by the cytochemical diaminobenzidine reaction. The reaction product obtained by preincubation with 3,3'-diaminobenzidine and incubation with the reagent and H(2)O(2) is distributed uniformly throughout the matri...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.53.2.276

    authors: Bieglmayer C,Ruis H

    更新日期:1974-02-01 00:00:00

  • The sugar-dependent1 lipase limits triacylglycerol accumulation in vegetative tissues of Arabidopsis.

    abstract::There has been considerable interest recently in the prospect of engineering crops to produce triacylglycerol (TAG) in their vegetative tissues as a means to achieve a step change in oil yield. Here, we show that disruption of TAG hydrolysis in the Arabidopsis (Arabidopsis thaliana) lipase mutant sugar-dependent1 (sdp...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.219840

    authors: Kelly AA,van Erp H,Quettier AL,Shaw E,Menard G,Kurup S,Eastmond PJ

    更新日期:2013-07-01 00:00:00

  • Modulation of Host Gene Expression during Initiation and Early Growth of Nodules in Cowpea, Vigna unguiculata (L.) Walp.

    abstract::Inoculation of 2-day-old cowpea (Vigna unguiculata [L.] Walp.) seedlings with Rhizobium fredii USDA257 results in proficient nodulation of the tap root. The most abundant nodulation occurs in a region roughly corresponding to the position of the root tip at the time of inoculation. We have examined plant gene expressi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.92.4.946

    authors: Trese AT,Pueppke SG

    更新日期:1990-04-01 00:00:00

  • Differential inhibition and activation of two leaf dihydroxyacetone phosphate reductases : role of fructose 2,6-bisphosphate.

    abstract::The chloroplastic and cytosolic forms of spinach (Spinacia oleracea cv Long Standing Bloomsdale) leaf NADH:dihydroxyacetone phosphate (DHAP) reductase were separated and partially purified. The chloroplastic form was stimulated by dithiothreitol, reduced thioredoxin, dihydrolipoic acid, 6-phosphogluconate, and phospha...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.2.379

    authors: Gee RW,Byerrum RU,Gerber DW,Tolbert NE

    更新日期:1988-06-01 00:00:00

  • Modulation of PSI and PSII Organization During Loss and Repair of Photosynthetic Activity in a Temperature Sensitive Mutant of Chlorella pyrenoidosa.

    abstract::Photosynthetic activity and organization of chlorophyll(Chl)-protein complexes in a temperature sensitive mutant of Chlorella pyrenoidosa have been investigated. The mutant is practically indistinguishable from wild type cells when grown at 25 C. However, mutant cells grown at 33 C do not synthesize Chl and lose their...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.6.1264

    authors: Lavintman N,Galling G,Ohad I

    更新日期:1981-12-01 00:00:00

  • Kinetic and spectral resolution of multiple nonphotochemical quenching components in Arabidopsis leaves.

    abstract::Using novel specially designed instrumentation, fluorescence emission spectra were recorded from Arabidopsis (Arabidopsis thaliana) leaves during the induction period of dark to high-light adaptation in order to follow the spectral changes associated with the formation of nonphotochemical quenching. In addition to an ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.148213

    authors: Lambrev PH,Nilkens M,Miloslavina Y,Jahns P,Holzwarth AR

    更新日期:2010-03-01 00:00:00

  • Water uptake, diameter change, and nonlinear diffusion in tree stems.

    abstract::A diffusion model for phloem swelling and contraction is proposed in which the rate of water movement changes markedly with moisture content. Good agreement between the actual swelling of the phloem of cotton stems and that predicted by the model was obtained. This result implies that water moves more readily into the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.2.247

    authors: Parlange JY,Turner NC,Waggoner PE

    更新日期:1975-02-01 00:00:00

  • Sugar Potentiation of Fatty Acid and Triacylglycerol Accumulation.

    abstract::Photosynthetically derived sugar provides carbon skeletons for lipid biosynthesis. We used mutants of Arabidopsis (Arabidopsis thaliana) and the expression of oleogenic factors to investigate relationships among sugar availability, lipid synthesis, and the accumulation of triacylglycerol (TAG) in leaf tissue. The adg1...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00828

    authors: Zhai Z,Liu H,Xu C,Shanklin J

    更新日期:2017-10-01 00:00:00

  • Photorespiration in c(3) and c(4) plant tissue cultures: significance of kranz anatomy to low photorespiration in c(4) plants.

    abstract::Photorespiration rates in tissue cultures of a C(4) plant, Portulaca oleracea, were compared to those in tissue cultures of a C(3) plant, Streptanthus tortuosus. The C(4) plant tissue cultures have one-half to one-third the photorespiration rate of the C(3) plant tissue cultures and respond to varying O(2) concentrati...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.4.573

    authors: Kennedy RA

    更新日期:1976-10-01 00:00:00

  • Transient accumulation of asparagine in sycamore cells after a long period of sucrose starvation.

    abstract::The mobilization of stored carbohydrates (sucrose and starch) and proteins during sucrose starvation was studied with sycamore (Acer pseudoplatanus L.) cells. When almost all the intracellular carbohydrate pools had disappeared, the cell protein content declined progressively whereas asparagine determined by either (1...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.2.717

    authors: Genix P,Bligny R,Martin JB,Douce R

    更新日期:1990-10-01 00:00:00

  • Going with the Flow: Multiscale Insights into the Composite Nature of Water Transport in Roots.

    abstract::As water often limits crop production, a more complete understanding of plant water capture and transport is necessary. Here, we developed MECHA, a mathematical model that computes the flow of water across the root at the scale of walls, membranes, and plasmodesmata of individual cells, and used it to test hypotheses ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.01006

    authors: Couvreur V,Faget M,Lobet G,Javaux M,Chaumont F,Draye X

    更新日期:2018-12-01 00:00:00

  • Iron acquisition by phytosiderophores contributes to cadmium tolerance.

    abstract::Based on the ability of phytosiderophores to chelate other heavy metals besides iron (Fe), phytosiderophores were suggested to prevent graminaceous plants from cadmium (Cd) toxicity. To assess interactions between Cd and phytosiderophore-mediated Fe acquisition, maize (Zea mays) plants were grown hydroponically under ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.094474

    authors: Meda AR,Scheuermann EB,Prechsl UE,Erenoglu B,Schaaf G,Hayen H,Weber G,von Wirén N

    更新日期:2007-04-01 00:00:00

  • Developmental regulation of enzymes of sucrose and hexose metabolism in effective and ineffective soybean nodules.

    abstract::Soybean (Glycine max) nodules formed by inoculation with either an effective strain or an ineffective (noninvasive, nodule-forming) strain of Bradyrhizobium japonicum were assayed for changes in developmental patterns of carbon metabolic enzymes of the plant nodule cells. Of the enzyme activities measured, only sucros...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.92.2.346

    authors: Anthon GE,Emerich DW

    更新日期:1990-02-01 00:00:00

  • The grapevine root-specific aquaporin VvPIP2;4N controls root hydraulic conductance and leaf gas exchange under well-watered conditions but not under water stress.

    abstract::We functionally characterized the grape (Vitis vinifera) VvPIP2;4N (for Plasma membrane Intrinsic Protein) aquaporin gene. Expression of VvPIP2;4N in Xenopus laevis oocytes increased their swelling rate 54-fold. Northern blot and quantitative reverse transcription-polymerase chain reaction analyses showed that VvPIP2;...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.203455

    authors: Perrone I,Gambino G,Chitarra W,Vitali M,Pagliarani C,Riccomagno N,Balestrini R,Kaldenhoff R,Uehlein N,Gribaudo I,Schubert A,Lovisolo C

    更新日期:2012-10-01 00:00:00

  • Promotive effects of organic solvents and kinetin on dark germination of lettuce seeds.

    abstract::Significant promotion in dark germination was observed when Grand Rapids lettuce (Lactuca sativa L.) seeds were soaked in acetone or dichloromethane, vacuum-dried, and imbibed at 25 C. Permeation of kinetin via these organic solvents further enhanced the dark germination. Those seeds that were affected by acetone and ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.3.446

    authors: Rao VS,Braun JW,Khan AA

    更新日期:1976-03-01 00:00:00

  • Long-Term Growth of Moss in Microfluidic Devices Enables Subcellular Studies in Development.

    abstract::Key developmental processes that occur on the subcellular and cellular level or occur in occluded tissues are difficult to access, let alone image and analyze. Recently, culturing living samples within polydimethylsiloxane (PDMS) microfluidic devices has facilitated the study of hard-to-reach developmental events. Her...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00879

    authors: Bascom CS Jr,Wu SZ,Nelson K,Oakey J,Bezanilla M

    更新日期:2016-09-01 00:00:00

  • The occurrence of phytoferritin and its relationship to effectiveness of soybean nodules.

    abstract::Polyacrylamide gel electrophoresis and electron microscopy revealed that accumulation of iron-protein in soybean nodules is influenced by nodule age, mutation in bradyrhizobia, and rhizobial/bradyrhizobial strain-soybean cultivar interactions. Iron-protein concentrations (micrograms per milligram protein) were inverse...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.2.299

    authors: Ko MP,Huang PY,Huang JS,Barker KR

    更新日期:1987-02-01 00:00:00

  • Physiological Comparisons of Pith Callus With Crown-Gall and Genetic Tumors of Nicotiana glauca, N. langsdorffii, and N. glauca-langsdorffii Grown in Vitro. II. Nutritional Physiology.

    abstract::Explants of genetic tumors, tumors initiated by Agrobacterium tumefaciens strains B-6 and T-37, and excised pith plugs from Nicotiana glauca, N. langsdorffii, and N. glauca-langsdorffii were cultured on Murashige and Skoog medium. All cultures, pith callus and tumors with the exception of N. langsdorffii pith grew on ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.7.1073

    authors: Sharp WR,Gunckel JE

    更新日期:1969-07-01 00:00:00

  • A cross-polarization, magic-angle-spinning, 13C-nuclear-magnetic-resonance study of polysaccharides in sugar beet cell walls

    abstract::Solid-state nuclear magnetic resonance relaxation experiments were used to study the rigidity and spatial proximity of polymers in sugar beet (Beta vulgaris) cell walls. Proton T1rho decay and cross-polarization patterns were consistent with the presence of rigid, crystalline cellulose microfibrils with a diameter of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.119.4.1315

    authors: Renard CM,Jarvis MC

    更新日期:1999-04-01 00:00:00

  • Light-induced Ethylene Production in Sorghum.

    abstract::Ethylene production was induced in sections of dark-grown Sorghum vulgare L. seedlings by treatment with light in the blue and far red regions of the light spectrum. The action spectrum closely resembled the previously reported spectra for high irradiance response; thus, light-induced ethylene production is probably a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.51.6.1082

    authors: Craker LE,Abeles FB,Shropshire W

    更新日期:1973-06-01 00:00:00

  • Gibberellins in Suspensors of Phaseolus coccineus L. Seeds.

    abstract::Analysis of extracts from 6300 (1.2 grams fresh weight) Phaseolus coccineus suspensors by combined gas chromatography-mass spectrometry has demonstrated the presence of five C(19)-gibberellins, GA(1), GA(4), GA(5), GA(6), GA(8), and one C(20)-GA, GA(44). The major GAs present were GA(1) and GA(8). Data are discussed i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.1.298

    authors: Picciarelli P,Alpi A

    更新日期:1986-09-01 00:00:00

  • Phototropism and photoinhibition of basipolar transport of auxin in oat coleoptiles.

    abstract::We have proposed that the lateral inequality of auxin associated with the phototropic response is a consequence of a light-induced impairment of basipolar transport of the hormone. If this is so, the dose-response curve for photoinhibition of auxin transport should resemble that for phototropism. Further, the waveleng...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.4.491

    authors: Shen-Miller J,Cooper P,Gordon SA

    更新日期:1969-04-01 00:00:00

  • Effect of cold acclimation on bulk tissue electrical impedance: I. Measurements with birdsfoot trefoil at subfreezing temperatures.

    abstract::The resistive and reactive components of electrical impedance were measured for birdsfoot trefoil (Lotus corniculatus L.) stems at freezing temperatures to -8 degrees C. As temperature decreased the specific resistance at frequencies between 49 hertz and 1.11 megahertz of stems from cold acclimated plants increased mo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.1.275

    authors: Stout DG

    更新日期:1988-01-01 00:00:00

  • Reduction of light-induced anthocyanin accumulation in inoculated sorghum mesocotyls. Implications for a compensatory role in the defense response.

    abstract::Sorghum (Sorghum bicolor L. Moench) accumulates the anthocyanin cyanidin 3-dimalonyl glucoside in etiolated mesocotyls in response to light. Inoculation with the nonpathogenic fungus Cochliobolus heterostrophus drastically reduced the light-induced accumulation of anthocyanin by repressing the transcription of the ant...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.3.979

    authors: Lo SC,Nicholson RL

    更新日期:1998-03-01 00:00:00

  • Resemblance and dissemblance of Arabidopsis type II peroxiredoxins: similar sequences for divergent gene expression, protein localization, and activity.

    abstract::The Arabidopsis type II peroxiredoxin (PRXII) family is composed of six different genes, five of which are expressed. On the basis of the nucleotide and protein sequences, we were able to define three subgroups among the PRXII family. The first subgroup is composed of AtPRXII-B, -C, and -D, which are highly similar an...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.022533

    authors: Bréhélin C,Meyer EH,de Souris JP,Bonnard G,Meyer Y

    更新日期:2003-08-01 00:00:00

  • Photosynthetic Efficiency as Bioindicator of Environmental Pressure in A. halleri.

    abstract::In earlier ecophysiological studies that were conducted on Arabidopsis halleri plants, scientists focused on the mechanisms of Cd and Zn hyperaccumulation but did not take into consideration the environmental factors that can significantly affect the physiological responses of plants in situ. In this study, we investi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00212

    authors: Sitko K,Rusinowski S,Kalaji HM,Szopiński M,Małkowski E

    更新日期:2017-09-01 00:00:00

  • Piperonylic acid, a selective, mechanism-based inactivator of the trans-cinnamate 4-hydroxylase: A new tool to control the flux of metabolites in the phenylpropanoid pathway

    abstract::Piperonylic acid (PA) is a natural molecule bearing a methylenedioxy function that closely mimics the structure of trans-cinnamic acid. The CYP73A subfamily of plant P450s catalyzes trans-cinnamic acid 4-hydroxylation, the second step of the general phenylpropanoid pathway. We show that when incubated in vitro with ye...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.118.1.209

    authors: Schalk M,Cabello-Hurtado F,Pierrel MA,Atanossova R,Saindrenan P,Werck-Reichhart D

    更新日期:1998-09-01 00:00:00

  • A transcriptome-based characterization of habituation in plant tissue culture.

    abstract::For the last 50 years, scientists have recognized that varying ratios of the plant hormones cytokinin and auxin induce plant cells to form particular tissues: undifferentiated calli, shoot structures, root structures, or a whole plant. Proliferation of undifferentiated callus tissue, greening, and the formation of sho...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.076059

    authors: Pischke MS,Huttlin EL,Hegeman AD,Sussman MR

    更新日期:2006-04-01 00:00:00

  • Biosynthetic Origin of Gibberellins A(3) and A(7) in Cell-Free Preparations from Seeds of Marah macrocarpus and Malus domestica.

    abstract::Cell-free preparations from seeds of Marah macrocarpus L. and Malus domestica L. catalyzed the conversion of gibberellin A(9) (GA(9)) and 2,3-dehydroGA(9) to GA(7); GA(9) was also metabolized to GA(4) in a branch pathway. The preparation from Marah seeds also metabolized GA(5) to GA(3) in high yield; GA(6) was a minor...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.1.132

    authors: Albone KS,Gaskin P,Macmillan J,Phinney BO,Willis CL

    更新日期:1990-09-01 00:00:00