Nitrate Reduction by Roots of Soybean (Glycine max [L.] Merr.) Seedlings.

Abstract:

:Studies were conducted with 9 to 12 day-old soybean (Glycine max [L.] Merr. cv. Williams) seedlings to determine the contribution of roots to whole plant NO(3) (-) reduction. Using an in vivo -NO(3) (-) nitrate reductase (NR) assay (no exogenous NO(3) (-) added to incubation medium) developed for roots, the roots accounted for approximately 30% of whole plant nitrate reductase activity (NRA) of plants grown on 15 mm NO(3) (-).Nitrogen analyses of xylem exudate showed that 53 to 66% of the total-N was as reduced-N, depending on the time of day of exudate collection. These observations supported enzyme data that suggested roots were contributing significantly to whole plant NO(3) (-) reduction. In short-term feeding studies using (15)N-NO(3) (-) significant and increasing atom percent (15)N excess was found in the reduced-N fraction of xylem exudate at 1.5 and 3 hours after feeding, respectively, which verified that roots were capable of reducing NO(3) (-).Estimated reduced-N accumulation by plants based on in vivo -NO(3) (-) NR assays of all plant parts substantially over-estimated actual reduced-N accumulation by the plants. Thus, the in vivo NR assay cannot be used to accurately estimate reduced-N accumulation but still serves as a useful assay for relative differences in treatment conditions.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Crafts-Brandner SJ,Harper JE

doi

10.1104/pp.69.6.1298

subject

Has Abstract

pub_date

1982-06-01 00:00:00

pages

1298-303

issue

6

eissn

0032-0889

issn

1532-2548

journal_volume

69

pub_type

杂志文章
  • Auxin and ROP GTPase Signaling of Polar Nuclear Migration in Root Epidermal Hair Cells.

    abstract::Polar nuclear migration is crucial during the development of diverse eukaryotes. In plants, root hair growth requires polar nuclear migration into the outgrowing hair. However, knowledge about the dynamics and the regulatory mechanisms underlying nuclear movements in root epidermal cells remains limited. Here, we show...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00713

    authors: Nakamura M,Claes AR,Grebe T,Hermkes R,Viotti C,Ikeda Y,Grebe M

    更新日期:2018-01-01 00:00:00

  • Cold Acclimation and Freezing Tolerance (A Complex Interaction of Light and Temperature).

    abstract::By comparing growth under five different temperature and irradiance regimes (20[deg]C and 800, 250, and 50[mu]mol m-2 s-1 and 5[deg]C and 250 and 50 [mu]mul m-2 s-1), we have examined the effects of light, temperature, and the relative reduction state of photosystem II on plant morphology, freezing tolerance (lethal t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.2.467

    authors: Gray GR,Chauvin LP,Sarhan F,Huner N

    更新日期:1997-06-01 00:00:00

  • Cell Wall Metabolism in Ripening Fruit (VII. Biologically Active Pectin Oligomers in Ripening Tomato (Lycopersicon esculentum Mill.) Fruits).

    abstract::A water-soluble, ethanol-insoluble extract of autolytically inactive tomato (Lycopersicon esculentum Mill.) pericarp tissue contains a series of galacturonic acid-containing (pectic) oligosaccharides that will elicit a transient increase in ethylene biosynthesis when applied to pericarp discs cut from mature green fru...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.2.575

    authors: Melotto E,Greve LC,Labavitch JM

    更新日期:1994-10-01 00:00:00

  • Effect of high temperature on calcium uptake by suspension-cultured pear fruit cells.

    abstract::Uptake of Ca(2+) by suspension-cultured pear (Pyrus communis L. cv Passe Crassane) cells and protoplasts was significantly enhanced by exposure to 38 degrees C compared to 25 degrees C. The increased uptake was specific for Ca(2+) and was not due to cell wall binding. Tissues pretreated at 38 degrees C showed increase...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.1.153

    authors: Klein JD,Ferguson IB

    更新日期:1987-05-01 00:00:00

  • Overexpression of ca1pase Decreases Rubisco Abundance and Grain Yield in Wheat.

    abstract::Rubisco catalyzes the fixation of CO2 into organic compounds that are used for plant growth and the production of agricultural products, and specific sugar-phosphate derivatives bind tightly to the active sites of Rubisco, locking the enzyme in a catalytically inactive conformation. 2-carboxy-d-arabinitol-1-phosphate ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00693

    authors: Lobo AKM,Orr DJ,Gutierrez MO,Andralojc PJ,Sparks C,Parry MAJ,Carmo-Silva E

    更新日期:2019-10-01 00:00:00

  • Phloem loading in two Scrophulariaceae species. What can drive symplastic flow via plasmodesmata?

    abstract::To determine the driving forces for symplastic sugar flux between mesophyll and phloem, gradients of sugar concentrations and osmotic pressure were studied in leaf tissues of two Scrophulariaceae species, Alonsoa meridionalis and Asarina barclaiana. A. meridionalis has a typical symplastic configuration of minor-vein ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.068312

    authors: Voitsekhovskaja OV,Koroleva OA,Batashev DR,Knop C,Tomos AD,Gamalei YV,Heldt HW,Lohaus G

    更新日期:2006-01-01 00:00:00

  • Overexpression of ARGOS Genes Modifies Plant Sensitivity to Ethylene, Leading to Improved Drought Tolerance in Both Arabidopsis and Maize.

    abstract::Lack of sufficient water is a major limiting factor to crop production worldwide, and the development of drought-tolerant germplasm is needed to improve crop productivity. The phytohormone ethylene modulates plant growth and development as well as plant response to abiotic stress. Recent research has shown that modify...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00780

    authors: Shi J,Habben JE,Archibald RL,Drummond BJ,Chamberlin MA,Williams RW,Lafitte HR,Weers BP

    更新日期:2015-09-01 00:00:00

  • Structural and Functional Insights into a Lysine Deacylase in the Cyanobacterium Synechococcus sp. PCC 7002.

    abstract::Lys deacylases are essential regulators of cell biology in many contexts. Here, we have identified CddA (cyanobacterial deacetylase/depropionylase), a Lys deacylase enzyme expressed in the cyanobacterium Synechococcus sp. PCC 7002 that has both deacetylase and depropionylase activity. Loss of the gene cddA led to slow...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00583

    authors: Liu X,Yang M,Liu Y,Ge F,Zhao J

    更新日期:2020-10-01 00:00:00

  • A Functional Unfolded Protein Response Is Required for Normal Vegetative Development.

    abstract::The unfolded protein response (UPR) is activated in plants in response to endoplasmic reticulum stress and plays an important role in mitigating stress damage. Multiple factors act in the UPR, including the membrane-associated transcription factor, BASIC LEUCINE ZIPPER 17 (bZIP17), and the membrane-associated RNA spli...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.01261

    authors: Bao Y,Bassham DC,Howell SH

    更新日期:2019-04-01 00:00:00

  • Effects of Previous Pollination and Stylar Ethylene on Pollen Tube Growth in Petunia hybrida Styles.

    abstract::The effect of ethylene on the growth rate of pollen tubes in styles of Petunia hybrida was examined. Apart from its strong inhibition of pollination-induced ethylene synthesis, aminoethoxyvinylglycine, placed on the stigma, did not impede tube growth. The inhibitors of the action of ethylene, silver thiosulfate and 2,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.1.4

    authors: Hoekstra FA,van Roekel T

    更新日期:1988-01-01 00:00:00

  • Virus-based microRNA expression for gene functional analysis in plants.

    abstract::Traditional virus-induced gene silencing (VIGS) is a powerful virus-based short interfering RNA-mediated RNA silencing technique for plant functional genomics. Besides short interfering RNAs, microRNAs (miRNAs) have also been shown to regulate gene expression by RNA silencing in various organisms. However, plant virus...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.155796

    authors: Tang Y,Wang F,Zhao J,Xie K,Hong Y,Liu Y

    更新日期:2010-06-01 00:00:00

  • The Arabidopsis family GT43 glycosyltransferases form two functionally nonredundant groups essential for the elongation of glucuronoxylan backbone.

    abstract::There exist four members of family GT43 glycosyltransferases in the Arabidopsis (Arabidopsis thaliana) genome, and mutations of two of them, IRX9 and IRX14, have previously been shown to cause a defect in glucuronoxylan (GX) biosynthesis. However, it is currently unknown whether IRX9 and IRX14 perform the same biochem...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.155309

    authors: Lee C,Teng Q,Huang W,Zhong R,Ye ZH

    更新日期:2010-06-01 00:00:00

  • Immunological Comparisons of Chymotrypsin Inhibitor I among Several Genera of the Solanaceae.

    abstract::Microcomplement fixation was employed to compare the immunological differences that occur between purified inhibitor I from potato tubers, the four purified protomers that comprise it, and inhibitor I from tuber and leaf extracts. Total inhibitors of chymotrypsin and trypsin in leaves of seven genera of the Solanaceae...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.5.627

    authors: Gurusiddaiah S,Kuo T,Ryan CA

    更新日期:1972-11-01 00:00:00

  • Stable transformation of ferns using spores as targets: Pteris vittata and Ceratopteris thalictroides.

    abstract::Ferns (Pteridophyta) are very important members of the plant kingdom that lag behind other taxa with regards to our understanding of their genetics, genomics, and molecular biology. We report here, to our knowledge, the first instance of stable transformation of fern with recovery of transgenic sporophytes. Spores of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.224675

    authors: Muthukumar B,Joyce BL,Elless MP,Stewart CN Jr

    更新日期:2013-10-01 00:00:00

  • Analysis of pea chloroplast inner and outer envelope membrane proteins by two-dimensional gel electrophoresis and their comparison with stromal proteins.

    abstract::Analysis of inner and outer pea (Pisum sativum var. Laxtons Progress No. 9) chloroplast envelope membranes by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that, although the two membranes have distinct polypeptide compositions, there are several comigrating polypeptides in the two membrane fracti...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.3.569

    authors: Werner-Washburne M,Cline K,Keegstra K

    更新日期:1983-11-01 00:00:00

  • Effects of CO(2) Concentration on Rubisco Activity, Amount, and Photosynthesis in Soybean Leaves.

    abstract::Growth at an elevated CO(2) concentration resulted in an enhanced capacity for soybean (Glycine max L. Merr. cv Bragg) leaflet photosynthesis. Plants were grown from seed in outdoor controlled-environment chambers under natural solar irradiance. Photosynthetic rates, measured during the seed filling stage, were up to ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.4.1310

    authors: Campbell WJ,Allen LH,Bowes G

    更新日期:1988-12-01 00:00:00

  • Correlation of Rapid Cell Death with Metabolic Changes in Fungus-Infected, Cultured Parsley Cells.

    abstract::To study in detail the hypersensitive reaction, one of the major defense responses of plants against microbial infection, we used a model system of reduced complexity with cultured parsley (Petroselinum crispum) cells infected with the phytopathogenic fungus Phytophthora infestans. Experimental conditions were establi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.1.433

    authors: Naton B,Hahlbrock K,Schmelzer E

    更新日期:1996-09-01 00:00:00

  • Whole genome analysis of gene expression reveals coordinated activation of signaling and metabolic pathways during pollen-pistil interactions in Arabidopsis.

    abstract::Plant reproduction depends on the concerted activation of many genes to ensure correct communication between pollen and pistil. Here, we queried the whole transcriptome of Arabidopsis (Arabidopsis thaliana) in order to identify genes with specific reproductive functions. We used the Affymetrix ATH1 whole genome array ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.169813

    authors: Boavida LC,Borges F,Becker JD,Feijó JA

    更新日期:2011-04-01 00:00:00

  • Citrate cleavage enzymes from developing soybean cotyledons: incorporation of citrate carbon into Fatty acids.

    abstract::Data are presented which demonstrate a citrate cleavage enzyme in the supernatant of a developing soybean (Glycine max L. Merr., var. Harosoy 63) cotyledon homogenate following a 126,000g spin for 2 hours. Activity of the enzyme was observed directly in the supernatant enzyme preparation and in a desalted supernatant ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.1.69

    authors: Nelson DR,Rinne RW

    更新日期:1975-01-01 00:00:00

  • Mediation of a plant response to malformin by ethylene.

    abstract::Malformin and ethylene stimulate abscission of the primary leaves of Phaseolus aureus Roxb. in the dark, and abscission stimulation by both compounds is inhibited by indeleacetic acid and CO(2). Ethylene production by malformin-treated buds is stimulated within 4 hours. and up to 8 days, after treatment. Malformin-ind...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.1.76

    authors: Curtis RW

    更新日期:1968-01-01 00:00:00

  • Developmental physiology of cluster-root carboxylate synthesis and exudation in harsh hakea. Expression of phosphoenolpyruvate carboxylase and the alternative oxidase.

    abstract::Harsh hakea (Hakea prostrata R.Br.) is a member of the Proteaceae family, which is highly represented on the extremely nutrient-impoverished soils in southwest Australia. When phosphorus is limiting, harsh hakea develops proteoid or cluster roots that release carboxylates that mobilize sparingly soluble phosphate in t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.035659

    authors: Shane MW,Cramer MD,Funayama-Noguchi S,Cawthray GR,Millar AH,Day DA,Lambers H

    更新日期:2004-05-01 00:00:00

  • Bisphosphonate inhibitors reveal a large elasticity of plastidic isoprenoid synthesis pathway in isoprene-emitting hybrid aspen.

    abstract::Recently, a feedback inhibition of the chloroplastic 1-deoxy-D-xylulose 5-phosphate (DXP)/2-C-methyl-D-erythritol 4-phosphate (MEP) pathway of isoprenoid synthesis by end products dimethylallyl diphosphate (DMADP) and isopentenyl diphosphate (IDP) was postulated, but the extent to which DMADP and IDP can build up is n...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00470

    authors: Rasulov B,Talts E,Kännaste A,Niinemets Ü

    更新日期:2015-06-01 00:00:00

  • Low Temperature Enhances Plant Immunity via Salicylic Acid Pathway Genes That Are Repressed by Ethylene.

    abstract::Temperature has a large impact on plant immune responses. Earlier studies identified intracellular immune receptor nucleotide-binding leucine-rich repeat (NLR) genes and salicylic acid (SA) as targets of high-temperature inhibition of plant immunity. Here, we report that moderately low temperature enhances immunity to...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.01130

    authors: Li Z,Liu H,Ding Z,Yan J,Yu H,Pan R,Hu J,Guan Y,Hua J

    更新日期:2020-01-01 00:00:00

  • Purification and Characterization of the Enzymes of Fructan Biosynthesis in Tubers of Helianthus tuberosus Colombia (II. Purification of Sucrose:Sucrose 1-Fructosyltransferase and Reconstitution of Fructan Synthesis in Vitro with Purified Sucrose:Sucrose

    abstract::Sucrose:sucrose 1-fructosyltransferase (1-SST), an enzyme involved in fructan biosynthesis, was purified to homogeneity from tubers of Helianthus tuberosus that were harvested in the accumulation phase. Gel filtration under native conditions predicted a molecular mass of about 67 kD. Electrophoresis or gel filtration ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.4.1167

    authors: Koops AJ,Jonker HH

    更新日期:1996-04-01 00:00:00

  • Chloroplast and mitochondrial proteases in Arabidopsis. A proposed nomenclature.

    abstract::The identity and scope of chloroplast and mitochondrial proteases in higher plants has only started to become apparent in recent years. Biochemical and molecular studies suggested the existence of Clp, FtsH, and DegP proteases in chloroplasts, and a Lon protease in mitochondria, although currently the full extent of t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.125.4.1912

    authors: Adam Z,Adamska I,Nakabayashi K,Ostersetzer O,Haussuhl K,Manuell A,Zheng B,Vallon O,Rodermel SR,Shinozaki K,Clarke AK

    更新日期:2001-04-01 00:00:00

  • Phosphorus Distribution in Red Pine Roots and the Ectomycorrhizal Fungus Hebeloma arenosa.

    abstract::Red pines (Pinus resinosa Ait.) were grown in a pasteurized sandy loam either unamended with phosphate or fertilized with one of two levels of phosphate (34 or 136 mg/kg) as superphosphate, and with and without addition of Hebeloma arenosa inoculum. Shoot and total dry weights of mycorrhizal seedlings grown in soil un...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.2.713

    authors: Macfall JS,Slack SA,Wehrli S

    更新日期:1992-10-01 00:00:00

  • Evolution of Cold Acclimation and Its Role in Niche Transition in the Temperate Grass Subfamily Pooideae.

    abstract::The grass subfamily Pooideae dominates the grass floras in cold temperate regions and has evolved complex physiological adaptations to cope with extreme environmental conditions like frost, winter, and seasonality. One such adaptation is cold acclimation, wherein plants increase their frost tolerance in response to gr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.01448

    authors: Schubert M,Grønvold L,Sandve SR,Hvidsten TR,Fjellheim S

    更新日期:2019-05-01 00:00:00

  • Phototropism and photoinhibition of basipolar transport of auxin in oat coleoptiles.

    abstract::We have proposed that the lateral inequality of auxin associated with the phototropic response is a consequence of a light-induced impairment of basipolar transport of the hormone. If this is so, the dose-response curve for photoinhibition of auxin transport should resemble that for phototropism. Further, the waveleng...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.4.491

    authors: Shen-Miller J,Cooper P,Gordon SA

    更新日期:1969-04-01 00:00:00

  • Iron uptake and translocation by tomato plants as influenced by root temperature and manganese nutrition.

    abstract::The uptake of iron (Fe) by VF-36 tomato plants as influenced by root temperature and manganese (Mn) nutrition was studied. Following a 1-week pretreatment period of various levels of Fe and Mn in the nutrient solution in a controlled temperature room, the uptake of (59)Fe from ferric ethylenediamine di(O-hydroxyphenyl...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.7.1095

    authors: Riekels JW,Lingle JC

    更新日期:1966-09-01 00:00:00

  • Mitochondrial malate dehydrogenase from corn : purification of multiple forms.

    abstract::A method to fractionate corn (Zea mays L. B73) mitochondria into soluble proteins, high molecular weight soluble proteins, and membrane proteins was developed. These fractions were analyzed by both sodium dodecyl sulfate-polyacrylamide gel electrophoresis and assays of mitochondrial enzyme activities. The Krebs cycle ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.97.4.1381

    authors: Hayes MK,Luethy MH,Elthon TE

    更新日期:1991-12-01 00:00:00