Abstract:
:Traditional virus-induced gene silencing (VIGS) is a powerful virus-based short interfering RNA-mediated RNA silencing technique for plant functional genomics. Besides short interfering RNAs, microRNAs (miRNAs) have also been shown to regulate gene expression by RNA silencing in various organisms. However, plant virus-based miRNA silencing has not been reported. In addition, a number of plant miRNAs have been identified or predicted, while their functions are largely unknown. Thus, there is an urgent need for the development of new technologies to study miRNA function. Here, we report that a modified cabbage leaf-curl geminivirus vector can be used to express artificial and endogenous miRNAs in plants. Using this viral miRNA expression system, we demonstrate that VIGS using artificial miRNAs, dubbed as "MIR VIGS," was effective to silence the expression of endogenous genes, including PDS, Su, CLA1, and SGT1, in Nicotiana benthamiana. Silencing of SGT1 led to the loss of N-mediated resistance to Tobacco mosaic virus. Furthermore, using this viral miRNA expression system, we found that viral ectopic expression of endogenous miR156 and miR165 but not their mutants in N. benthamiana resulted in earlier abnormal developmental phenotypes, and expression of miR165 induced abnormal chlorotic spots on leaves. These results demonstrate that the cabbage leaf-curl geminivirus-based miRNA expression system can be utilized not only to specifically silence genes involved in general metabolism and defense but also to investigate the function of endogenous miRNAs in plants.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Tang Y,Wang F,Zhao J,Xie K,Hong Y,Liu Ydoi
10.1104/pp.110.155796subject
Has Abstractpub_date
2010-06-01 00:00:00pages
632-41issue
2eissn
0032-0889issn
1532-2548pii
pp.110.155796journal_volume
153pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::A mathematical model is presented to explain the regulation of nitrogenase electron allocation to N2 fixation (EAC) in legume nodules. The model is based on two assumptions: (a) that H2 inhibits N2 fixation in a competitive manner; and (b) that O2, H2, and N2 move into and out of nodules by diffusion and their movemen...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.2.421
更新日期:1993-10-01 00:00:00
abstract::Infrared video thermography was used to observe ice nucleation temperatures, patterns of ice formation, and freezing rates in nonacclimated and cold acclimated leaves of a spring (cv Quest) and a winter (cv Express) canola (Brassica napus). Distinctly different freezing patterns were observed, and the effect of water ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.028308
更新日期:2004-07-01 00:00:00
abstract::In nature, plants interact with numerous beneficial or pathogenic soil-borne microorganisms. Plants have developed various defense strategies to expel pathogenic microbes, some of which function soon after pathogen infection. We used Medicago truncatula and its oomycete pathogen Aphanomyces euteiches to elucidate earl...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.00278
更新日期:2019-07-01 00:00:00
abstract::Water storage is thought to play an integral role in the maintenance of whole-plant water balance. The contribution of both living and dead cells to water storage can be derived from rehydration and water-release curves on excised plant material, but the underlying tissue-specific emptying/refilling dynamics remain un...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.01133
更新日期:2017-12-01 00:00:00
abstract::A phytostat to mass culture higher plant cells in liquid medium is described. This apparatus allowed the culture in batch, turbidostat and chemostat of 20 liters of cells. Automatic control of cell suspension growth was based on culture turbidity. Changes with time of certain cell characteristics, particularly cell re...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.59.3.502
更新日期:1977-03-01 00:00:00
abstract::The brown planthopper, Nilaparvata lugens, is a pest that threatens rice (Oryza sativa) production worldwide. While feeding on rice plants, planthoppers secrete saliva, which plays crucial roles in nutrient ingestion and modulating plant defense responses, although the specific functions of salivary proteins remain la...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00755
更新日期:2018-01-01 00:00:00
abstract::Saline-soluble glycinins and insoluble glutelins are the major storage proteins in soybean (Glycine max) and rice (Oryza sativa), respectively. In spite of their differences in solubility properties, both proteins are members of the 11S globulin gene family based on their similarities in primary sequences and processi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.120.4.1063
更新日期:1999-08-01 00:00:00
abstract::An investigation was made to determine the univalent cation requirements of starch synthetase from a variety of plant species of economic importance. The particulate enzyme from sweet corn was shown to have an absolute requirement for potassium, with the optimum activation occurring at 0.05 M KCl. Rubidium, cesium, an...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.9.1260
更新日期:1969-09-01 00:00:00
abstract::The phospholipid dependence of the UDP-glucose sterol glucosyl transferase (UDPG-SGTase) from maize coleoptiles was previously demonstrated using the partially purified and highly delipidated enzyme, in the presence of the detergent Triton X-100 (P Ullmann, P Bouvier-Navé, P Benveniste [1987] Plant Physiol 85: 51-55)....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.91.2.567
更新日期:1989-10-01 00:00:00
abstract::Three populations of seedlings of Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco) were subjected to recurrent drought cycles. Pressure-volume curves were developed to derive the osmotic and pressure potentials as functions of the symplastic water over a wide range of relative water content. The drought cycles induc...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.83.2.418
更新日期:1987-02-01 00:00:00
abstract::The Arabidopsis (Arabidopsis thaliana) orthologs of Brca2, a protein whose mutations are involved in breast cancer in humans, were previously shown to be essential at meiosis. In an attempt to better understand the Brca2-interacting properties, we examined four partners of the two isoforms of Brca2 identified in Arabi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.075838
更新日期:2006-03-01 00:00:00
abstract::Investigation on the biosynthesis of S-methyl-L-cysteine in radish leaves has shown that it is formed by the methylation of cysteine. This conclusion is based on: A) the relatively high recovery of radioactivity in methylcysteine sulfoxide after the administration of cysteine or methyl-labeled methionine to radish lea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.8.1301
更新日期:1966-10-01 00:00:00
abstract::Prevention of actin polymerization with low concentrations of latrunculin B (Lat-B; 2 nm) exerts a profound inhibitory effect on pollen tube growth. Using flow-through chambers, we show that growth retardation starts after 10 min treatment with 2 nm Lat-B, and by 15 to 20 min reaches a basal rate of 0.1 to 0.2 microm/...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.113035
更新日期:2008-04-01 00:00:00
abstract::We tested whether the expression of the suberization-associated anionic peroxidase gene is involved in the timely appearance of the vascular suberized coating involved in the resistance of a tomato line to Verticillium albo-atrum. The mRNA for this peroxidase appeared at a higher level one day earlier in wound-healing...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.92.1.276
更新日期:1990-01-01 00:00:00
abstract::Prolonged growth of cell cultures of sycamore (Acer pseudoplatanus L.) on agar medium containing myo-inositol-2-(3)H resulted in incorporation of label predominately into uronosyl and pentosyl units of cell wall polysaccharides. Procedures normally used to distinguish between pectic substance and hemicellulose yielded...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.9.1489
更新日期:1966-11-01 00:00:00
abstract::The intracellular distribution of enzymes capable of catalyzing the reactions from oxaloacetate to sucrose in germinating castor bean endosperm has been studied by sucrose density gradient centrifugation. One set of glycolytic enzyme activities was detected in the plastids and another in the cytosol. The percentages o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.1.31
更新日期:1979-07-01 00:00:00
abstract::Hydroperoxide lyase (HPLS) activity in soybean (Glycine max) seed/seedlings, leaves, and chloroplasts of leaves required detergent solubilization for maximum in vitro activity. On a per milligram of protein basis, more HPLS activity was found in leaves, especially chloroplasts, than in seeds or seedlings. The total yi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.97.3.1059
更新日期:1991-11-01 00:00:00
abstract::The chloroplast response in the green alga Dunaliella salina to irradiance stress was investigated. Cells were grown under low light (LL) at 100 [mu]mol photons m-2 s-1 or high light (HL) at 2000 [mu]mol photons m-2 s-1 incident intensity. LL-grown cells had a low chlorophyll (Chl) a/b ratio, an abundance of light-har...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.3.885
更新日期:1995-03-01 00:00:00
abstract::It has been suggested that jasmonic acid (JA) could be an integral part of a general signal transduction system regulating inducible defense genes in plants. It was reported that treatment with an elicitor (N-acetylchitoheptaose) induced production of phytoalexin in suspension-cultured rice (Oryza sativa L.) cells. In...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.2.387
更新日期:1996-02-01 00:00:00
abstract::The expression of nitrilase in Arabidopsis during the development of the clubroot disease caused by the obligate biotroph Plasmodiophora brassicae was investigated. A time course study showed that only during the exponential growth phase of the clubs was nitrilase prominently enhanced in infected roots compared with c...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.122.2.369
更新日期:2000-02-01 00:00:00
abstract::Highly purified fractions of three starch branching enzymes from developing maize (Zea mays L.) endosperm were used to prepare antisera in rabbits. In double diffusion experiments, no immunoprecipitate was observed when branching enzyme IIa or IIb was tested against branching enzyme I antiserum. No immunoprecipitate w...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.72.3.813
更新日期:1983-07-01 00:00:00
abstract::The enormous diversity of seed traits is an intriguing feature and critical for the overwhelming success of higher plants. In particular, seed mass is generally regarded to be key for seedling development but is mostly approximated by using scanning methods delivering only two-dimensional data, often termed seed size....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01122
更新日期:2016-11-01 00:00:00
abstract::The sink-source conversion in developing leaves of tobacco (Nicotiana tabacum L.) was studied to determine whether import termination is caused by the onset of export or is related to achievement of positive carbon balance. Albino shoots were grown in vitro and grafted to detopped stems of green tobacco plants. Termin...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.76.1.45
更新日期:1984-09-01 00:00:00
abstract::In an earlier paper we showed that in fully developed barley (Hordeum vulgare L.) root epidermal cells a decrease in cytosolic K+ was associated with an acidification of the cytosol (D.J. Walker, R. A. Leigh, A.J. Miller [1996] Proc Natl Acad Sci USA 93: 10510-10514). To show that these changes in cytosolic ion concen...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.118.3.957
更新日期:1998-11-01 00:00:00
abstract::The effects of plant hormones and sucrose (Suc) on potato (Solanum tuberosum L.) tuberization were studied using in vitro cultured single-node cuttings. Tuber-inducing (high Suc) and -noninducing (low Suc or high Suc plus gibberellin [GA]) media were tested. Tuberization frequencies, tuber widths, and stolon lengths w...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.117.2.575
更新日期:1998-06-01 00:00:00
abstract::Free amino acid constituents were determined of grape stem and Phylloxera leaf gall callus in tissue culture. Fast, medium and slow growing single cell clones of, respectively, stem and gall origins were grown on a mineral salt-sucrose medium supplemented with coconut milk and alpha-naphthaleneacetic acid. Stem and ga...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.4.573
更新日期:1966-04-01 00:00:00
abstract::Incubation of freshly cut carrot tissue in Na(3)ADP and Na(3)ATP promotes a marked intracellular increase in both ADP and ATP. The rapid increase in ATP during an ADP incubation and in ADP during an ATP incubation results from the activity of cytoplasmic enzyme systems upon the nucleotide absorbed into the cell from t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.45.4.500
更新日期:1970-04-01 00:00:00
abstract::The expression patterns of five genes (PsGA20ox1, PsGA20ox2, PsGA3ox1, PsGA2ox1, and PsGA2ox2) encoding five regulatory gibberellin (GA) biosynthesis enzymes (two GA 20-oxidases, a GA 3beta-hydroxylase, and two GA 2beta-hydroxylases) were examined to gain insight into how these genes coordinate GA biosynthesis during ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.086199
更新日期:2006-11-01 00:00:00
abstract::Rhizobium promoters involved in the formation of root nodules on leguminous plants are activated by flavonoids in plant root exudate. A series of Rhizobium strains which all contain the inducible Rhizobium leguminosarum nodA promoter fused to the Escherichia coli lacZ gene, and which differ only in the source of the r...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.4.1298
更新日期:1988-04-01 00:00:00
abstract::Endosperm halves from 3-day-old castor bean (Ricinus communis var Hale) were incubated for 30 minutes with l-[(14)C]serine, after which label was observed in ethanolamine, choline, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine, ethanolaminephosphate, and CDPethanolamine, but not in cholinephosphate...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.84.1.78
更新日期:1987-05-01 00:00:00