Interaction between Arabidopsis Brca2 and its partners Rad51, Dmc1, and Dss1.

Abstract:

:The Arabidopsis (Arabidopsis thaliana) orthologs of Brca2, a protein whose mutations are involved in breast cancer in humans, were previously shown to be essential at meiosis. In an attempt to better understand the Brca2-interacting properties, we examined four partners of the two isoforms of Brca2 identified in Arabidopsis (AtRad51, AtDmc1, and two AtDss1 isoforms). The two Brca2 and the two Dss1 isoforms are named AtBrca2(IV), AtBrca2(V), AtDss1(I), and AtDss1(V) after their chromosomal localization. We first show that both AtBrca2 proteins can interact with either AtRad51 or AtDmc1 in vitro, and that the N-terminal region of AtBrca2 is responsible for these interactions. More specifically, the BRC motifs (so called because iterated in the Brca2 protein) in Brca2 are involved in these interactions: BRC motif number 2 (BRC2) alone can interact with AtDmc1, whereas BRC motif number 4 (BRC4) recognizes AtRad51. The human Rad51 and Dmc1 proteins themselves can interact with either the complete (HsRad51) or a shorter version of AtBrca2 (HsRad51 or HsDmc1) that comprises all four BRC motifs. We also identified two Arabidopsis isoforms of Dss1, another known partner of Brca2 in other organisms. Although all four Brca2 and Dss1 proteins are much conserved, AtBrca2(IV) interacts with only one of these AtDss1 proteins, whereas AtBrca2(V) interacts with both of them. Finally, we show for the first time that an AtBrca2 protein could bind two different partners at the same time: AtRad51 and AtDss1(I), or AtDmc1 and AtDss1(I).

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Dray E,Siaud N,Dubois E,Doutriaux MP

doi

10.1104/pp.105.075838

subject

Has Abstract

pub_date

2006-03-01 00:00:00

pages

1059-69

issue

3

eissn

0032-0889

issn

1532-2548

pii

pp.105.075838

journal_volume

140

pub_type

杂志文章
  • ABA Uptake in Source and Sink Tissues of Sugar Beet.

    abstract::The mode of abscisic acid (ABA) uptake was studied in excised leaf and root tissue discs of sugar beet (Beta vulgaris L.). Discs were incubated in buffered medium that contained 1 mm CaCl(2) and [(14)C]ABA. The sensitivity of ABA uptake to metabolic inhibitors and temperature indicated that the ABA transport system ha...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.2.430

    authors: Daie J,Wyse R

    更新日期:1983-06-01 00:00:00

  • Onset of deoxyribonucleic Acid synthesis in germinating wheat embryos.

    abstract::Germinating wheat embryos (Triticum vulgare var. Florence) synthesize proteins before the onset of DNA synthesis. The onset of DNA replication occurs at about 15 hours of germination and was shown to depend on proteins synthesized before 9 hours of germination with the use of blasticidin S, a specific inhibitor of pro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.1.20

    authors: Mory YY,Chen D,Sarid S

    更新日期:1972-01-01 00:00:00

  • Regulation of Acyl Carrier Protein Messenger RNA Levels during Seed and Leaf Development.

    abstract::We have examined the expression of acyl carrier protein (ACP) mRNA levels and ACP activity in leaves, where fatty acids function primarily in membrane synthesis, and in developing soybean seeds, where fatty acids are primarily used for oil storage. An RNA probe transcribed from a synthetic spinach ACP-I gene hybridize...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.4.1174

    authors: Hannapel DJ,Ohlrogge JB

    更新日期:1988-04-01 00:00:00

  • Metabolism under Microaerobic Conditions of Mitochondria from Cowpea Nodules.

    abstract::A method is described for isolating mitochondria from nodules of cowpea (Vigna unguiculata [L.] Walp.) under completely anaerobic conditions. The mitochondria were immediately active when incubated aerobically with substrates, and their respiration rates were higher than mitochondria prepared in air. The mitochondria ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.81.4.1097

    authors: Rawsthorne S,Larue TA

    更新日期:1986-08-01 00:00:00

  • Protective function of chloroplast 2-cysteine peroxiredoxin in photosynthesis. Evidence from transgenic Arabidopsis.

    abstract::2-Cysteine peroxiredoxins (2-CPs) constitute a ubiquitous group of peroxidases that reduce cell-toxic alkyl hydroperoxides to their corresponding alcohols. Recently, we cloned 2-CP cDNAs from plants and characterized them as chloroplast proteins. To elucidate the physiological function of the 2-CP in plant metabolism,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.119.4.1407

    authors: Baier M,Dietz KJ

    更新日期:1999-04-01 00:00:00

  • Tolerance of photosynthesis to high temperature in desert plants.

    abstract::Winter- and summertime-active desert annual species were grown at different temperatures to assess their capacity for photosynthetic acclimation. Thermal stability of photosynthesis was determined from responses of chlorophyll fluorescence to increased temperature. Photosynthesis in winter ephemerals grown at 28 degre...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.4.786

    authors: Downton WJ,Berry JA,Seemann JR

    更新日期:1984-04-01 00:00:00

  • Development of the Primary Photochemical Apparatus of Photosynthesis during Greening of Etiolated Bean Leaves.

    abstract::Seven-day-old dark-grown bean leaves were greened under continuous light. The amount of chlorophyll, the ratio of chlorophyll a to chlorophyll b, the O(2) evolving capacity and the primary photochemical activities of Photosystem I and Photosystem II were measured on the leaves after various times of greening. The prim...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.4.526

    authors: Baker NR,Butler WL

    更新日期:1976-10-01 00:00:00

  • The ribosomes of pear fruit. Their synthesis during the climacteric and the age-related compensatory response to ionizing radiation.

    abstract::Maceration at liquid nitrogen temperatures, use of poly-vinylpyrrolidone, and careful pH control are essential to the isolation of ribosomes and polysomes from deciduous fruit tissue. Characteristics of the ribosomes and constituent RNA are described. The distribution of ribosomes among monomer and polymer forms remai...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.45.4.401

    authors: Ku LL,Romani RJ

    更新日期:1970-04-01 00:00:00

  • Specificities of the Acyl-Acyl Carrier Protein (ACP) Thioesterase and Glycerol-3-Phosphate Acyltransferase for Octadecenoyl-ACP Isomers (Identification of a Petroselinoyl-ACP Thioesterase in Umbelliferae).

    abstract::This study was designed to address the question: How specific for double bond position and conformation are plant enzymes that act on oleoyl-acyl carrier protein (ACP)? Octadecenoyl-ACPs with cis double bonds at positions [delta]6, [delta]7, [delta]8, [delta]9, [delta]10, [delta]11, or [delta]12 and elaidyl (18:1[delt...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.3.839

    authors: Dormann P,Frentzen M,Ohlrogge JB

    更新日期:1994-03-01 00:00:00

  • Induction and functional analysis of two reduced nicotinamide adenine dinucleotide phosphate-dependent glutathione peroxidase-like proteins in Synechocystis PCC 6803 during the progression of oxidative stress.

    abstract::Synechocystis PCC 6803 contains two types of glutathione peroxidase-like proteins (GPX-1 and GPX-2) that utilize NADPH but not reduced glutathione and unsaturated fatty acid hydroperoxides or alkyl hydroperoxides. The steady-state transcript level of gpx-1 gradually increased under oxidative stress conditions imposed ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.044842

    authors: Gaber A,Yoshimura K,Tamoi M,Takeda T,Nakano Y,Shigeoka S

    更新日期:2004-09-01 00:00:00

  • Purification and Characterization of Microsomal Cytochrome b(5) and NADH Cytochrome b(5) Reductase from Pisum sativum.

    abstract::In this communication we document the reproducible protocols for the purification of milligram quantities of cytochrome b(5) and NADH-cytochrome b(5) reductase from the microsomal fraction of Pisum sativum. The cytochrome b(5) component of this NADH linked electron transport chain was found to have a molecular mass of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.2.457

    authors: Jollie DR,Sligar SG,Schuler M

    更新日期:1987-10-01 00:00:00

  • Soybean root suberin: anatomical distribution, chemical composition, and relationship to partial resistance to Phytophthora sojae.

    abstract::Soybean (Glycine max L. Merr.) is a versatile and important agronomic crop grown worldwide. Each year millions of dollars of potential yield revenues are lost due to a root rot disease caused by the oomycete Phytophthora sojae (Kaufmann & Gerdemann). Since the root is the primary site of infection by this organism, we...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.091090

    authors: Thomas R,Fang X,Ranathunge K,Anderson TR,Peterson CA,Bernards MA

    更新日期:2007-05-01 00:00:00

  • Involvement of a Primary Electrogenic Pump in the Mechanism for HCO(3) Uptake by the Cyanobacterium Anabaena variabilis.

    abstract::The response of the membrane potential to HCO(3) (-) supply has been studied in the cyanobacterium Anabaena variabilis strain M-3 under various conditions. Changes in potential were followed with the aid of the lipophilic cation tetraphenyl phosphonium bromide.Addition of HCO(3) (-) to CO(2)-depleted cells resulted in...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.4.978

    authors: Kaplan A,Zenvirth D,Reinhold L,Berry JA

    更新日期:1982-04-01 00:00:00

  • Nicotine Biosynthetic Enzyme Activities in Nicotiana tabacum L. Genotypes with Different Alkaloid Levels.

    abstract::Young plants of five Nicotiana tabacum L. genotypes were examined for activity of nicotine biosynthetic enzymes. Genotypes near isogenic except at two loci each with two alleles controlling nicotine level were used in a comparison of the four homozygous allelic combinations producing high, high intermediate, low inter...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.64.2.236

    authors: Saunders JW,Bush LP

    更新日期:1979-08-01 00:00:00

  • An early response to gibberellic Acid not requiring protein synthesis.

    abstract::Cell-free extracts from gibberellic acid-treated barley (Hordeum vulgare L. cv. Himalaya) aleurone layers show phosphorylcholine glyceride transferase activity greater than that from control layers. The increase in activity is not prevented by a mixture of amino acid analogs nor by cordycepin under conditions in which...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.6.813

    authors: Ben-Tal Y

    更新日期:1974-12-01 00:00:00

  • An mRNA putatively coding for an O-methyltransferase accumulates preferentially in maize roots and is located predominantly in the region of the endodermis.

    abstract::ZRP4, a 1.4-kb mRNA that preferentially accumulates in roots of young Zea mays L. plants, was identified by isolation of the corresponding cDNA clone. Genomic Southern analysis indicates that the zrp4 gene is represented once in the corn genome. The deduced ZRP4 polypeptide of 39,558 D is rich in leucine, serine, and ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.3.1001

    authors: Held BM,Wang H,John I,Wurtele ES,Colbert JT

    更新日期:1993-07-01 00:00:00

  • Phytochrome-Mediated Light Regulation of PHYA- and PHYB-GUS Transgenes in Arabidopsis thaliana Seedlings.

    abstract::Phytochrome wild-type gene-[beta]-glucuronidase (PHY-GUS) gene fusions were used in transgenic Arabidopsis to compare the activity levels and light regulation of the PHYA and PHYB promoters and to identify the photoreceptors mediating this regulation. In dark-grown seedlings, both promoters are 4-fold more active in s...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.2.523

    authors: Somers DE,Quail PH

    更新日期:1995-02-01 00:00:00

  • Chlorophyll Formation and Photosynthetic Competence in Euglena During Light-Induced Chloroplast Development in the Presence of 3, (3,4-dichlorophenyl) 1,1-Dimethyl Urea (DCMU).

    abstract::The possibility that photosynthetic competence is gratuitous for light-induced chloroplast development in Euglena gracilis var. bacillaris was examined by incubating dark-grown resting cells in the light with DCMU, an inhibitor of photosynthesis. Under these conditions photosynthetic carbon dioxide fixation was inhibi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.12.1716

    authors: Schiff JA,Zeldin MH,Rubman J

    更新日期:1967-12-01 00:00:00

  • Characterization of MdMADS2, a member of the SQUAMOSA subfamily of genes, in apple.

    abstract::A MADS-box gene, MdMADS2, was isolated from the apple (Malus x domestica Borkh.) var Fuji and its developmental expression pattern was studied during flower development. MdMADS2 shares a high degree of amino acid sequence identity with the SQUAMOSA subfamily of genes. RNA blot analysis showed that MdMADS2 is transcrib...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.120.4.969

    authors: Sung SK,Yu GH,An G

    更新日期:1999-08-01 00:00:00

  • Photosynthetic pigment localization and thylakoid membrane morphology are altered in Synechocystis 6803 phycobilisome mutants.

    abstract::Cyanobacteria are oxygenic photosynthetic prokaryotes that are the progenitors of the chloroplasts of algae and plants. These organisms harvest light using large membrane-extrinsic phycobilisome antenna in addition to membrane-bound chlorophyll-containing proteins. Similar to eukaryotic photosynthetic organisms, cyano...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.192849

    authors: Collins AM,Liberton M,Jones HD,Garcia OF,Pakrasi HB,Timlin JA

    更新日期:2012-04-01 00:00:00

  • Functional genomics reveals that a compact terpene synthase gene family can account for terpene volatile production in apple.

    abstract::Terpenes are specialized plant metabolites that act as attractants to pollinators and as defensive compounds against pathogens and herbivores, but they also play an important role in determining the quality of horticultural food products. We show that the genome of cultivated apple (Malus domestica) contains 55 putati...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.208249

    authors: Nieuwenhuizen NJ,Green SA,Chen X,Bailleul EJ,Matich AJ,Wang MY,Atkinson RG

    更新日期:2013-02-01 00:00:00

  • Effects of low concentrations of o(3) on net photosynthesis, dark respiration, and chlorophyll contents in aging hybrid poplar leaves.

    abstract::Chronic exposure of hybrid poplar (Populus deltoides x trichocarpa) plants to low concentrations of ozone had negative impact upon net photosynthetic capacity, dark respiration, and leaf chlorophyll contents. Exposure to as much as 0.20 microliters per liter O(3) had no immediate effect on net photosynthesis (P(n)), b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.2.291

    authors: Reich PB

    更新日期:1983-10-01 00:00:00

  • ATP-Dependent Proteolytic Activity from Spinach Leaves.

    abstract::Spinach (Spinacia oleracea CV Bloomsdale Long Standing) leaf cytoplasmic starch phosphorylase and rabbit muscle phosphorylase a were inactivated by incubation with partially purified leaf extract in the presence of ATP and Mg(2+). The inactivating factor(s) were heat stable and susceptible to protease attack. Phosphor...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.4.902

    authors: Hammond JB,Preiss J

    更新日期:1983-12-01 00:00:00

  • Enhanced deoxyribonucleic Acid polymerase activity of chromatin from soybean hypocotyls treated with 2,4-dichlorophenoxyacetic Acid.

    abstract::Chromatin isolated from soybean (Glycine max L., var. Wayne) hypocotyls was capable of catalyzing the polymerization of labeled deoxyribonucleoside triphosphate in the presence of the three other deoxyribonucleoside triphosphates into a trichloroacetic acid-insoluble product. This product was insensitive to base hydro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.48.1.43

    authors: Leffler HR,O'brien TJ,Glover DV,Cherry JH

    更新日期:1971-07-01 00:00:00

  • Endogenous abscisic acid as a key switch for natural variation in flooding-induced shoot elongation.

    abstract::Elongation of leaves and stem is a key trait for survival of terrestrial plants during shallow but prolonged floods that completely submerge the shoot. However, natural floods at different locations vary strongly in duration and depth, and, therefore, populations from these locations are subjected to different selecti...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.162792

    authors: Chen X,Pierik R,Peeters AJ,Poorter H,Visser EJ,Huber H,de Kroon H,Voesenek LA

    更新日期:2010-10-01 00:00:00

  • Amino Acid Metabolism of Lemna minor L. : IV. N-Labeling Kinetics of the Amide and Amino Groups of Glutamine and Asparagine.

    abstract::A serious limitation to the use of N(O,S)-heptafluorobutyryl isobutyl amino acid derivatives in the analysis of (15)N-labeling kinetics of amino acids in plant tissues, is that the amides glutamine and asparagine undergo acid hydrolysis to glutamate and aspartate, respectively, during derivatization. This led us to co...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.4.1161

    authors: Rhodes D,Rich PJ,Brunk DG

    更新日期:1989-04-01 00:00:00

  • Light-regulated gravitropism in seedling roots of maize.

    abstract::Red light-induced changes in the gravitropism of roots of Zea mays variety Merit is a very low fluence response with a threshold of 10(-9) moles per square meter and is not reversible by far red light. Blue light also affects root gravitropism but the sensitivity of roots to blue is 50 to 100 times less than to an eq...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.2.241

    authors: Feldman LJ,Briggs WR

    更新日期:1987-01-01 00:00:00

  • Effect of heat shock on the metabolism of glutathione in maize roots.

    abstract::High performance liquid chromatography analyses revealed that glutathione (GSH) and cysteine are two of the major low molecular weight thiol compounds in maize root extracts. Treatment of maize roots to heat shock temperatures of 40 degrees C resulted in a decrease of cysteine levels and an increase of GSH levels. Pul...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.4.1031

    authors: Nieto-Sotelo J,Ho TH

    更新日期:1986-12-01 00:00:00

  • Passive nitrate transport by root plasma membrane vesicles exhibits an acidic optimal pH like the H(+)-ATPase.

    abstract::The net initial passive flux (J(Ni)) in reconstituted plasma membrane (PM) vesicles from maize (Zea mays) root cells was measured as recently described (P. Pouliquin, J.-P. Grouzis, R. Gibrat ¿1999 Biophys J 76: 360-373). J(Ni) in control liposomes responded to membrane potential or to NO(3)(-) as expected from the Go...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.1.265

    authors: Pouliquin P,Boyer JC,Grouzis JP,Gibrat R

    更新日期:2000-01-01 00:00:00

  • Partial Purification and Some Properties of Flavonol 7-Sulfotransferase from Flaveria bidentis.

    abstract::A novel flavonol-specific sulfotransferase was partially purified from the shoot tips of Flaveria bidentis var. Angustifolia O.K. (Asteraceae) by chromatography on 3'-phosphoadenosine 5'-phosphate-agarose affinity column and chromatofocusing on Mono P. The latter step resulted in the separation of two isoforms, both o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.4.1254

    authors: Varin L,Ibrahim RK

    更新日期:1991-04-01 00:00:00