Abstract:
:Chromatin isolated from soybean (Glycine max L., var. Wayne) hypocotyls was capable of catalyzing the polymerization of labeled deoxyribonucleoside triphosphate in the presence of the three other deoxyribonucleoside triphosphates into a trichloroacetic acid-insoluble product. This product was insensitive to base hydrolysis and ribonuclease, but was sensitive to acid hydrolysis and deoxyribonuclease. Chromatin-DNA polymerase required Mg(2+) and all four deoxyribonucleoside triphosphates for maximal activity. Inorganic pyrophosphate and actinomycin D inhibited the polymerase activity, but 2, 4-dichlorophenoxyacetic acid had no effect in vitro. Chromatin from plants previously treated with 2, 4-dichlorophenoxyacetic acid supported a greater level of DNA synthesis than did chromatin from untreated plants.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Leffler HR,O'brien TJ,Glover DV,Cherry JHdoi
10.1104/pp.48.1.43subject
Has Abstractpub_date
1971-07-01 00:00:00pages
43-5issue
1eissn
0032-0889issn
1532-2548journal_volume
48pub_type
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