Abstract:
:Optimization of nitrogen fixation by rhizobia in legumes is a key area of research for sustainable agriculture. Symbiotic nitrogen fixation (SNF) occurs in specialized organs called nodules and depends on a steady supply of carbon to both plant and bacterial cells. Here we report the functional characterization of a nodule-specific Suc transporter, MtSWEET11 from Medicago truncatula MtSWEET11 belongs to a clade of plant SWEET proteins that are capable of transporting Suc and play critical roles in pathogen susceptibility. When expressed in mammalian cells, MtSWEET11 transported sucrose (Suc) but not glucose (Glc). The MtSWEET11 gene was found to be expressed in infected root hair cells, and in the meristem, invasion zone, and vasculature of nodules. Expression of an MtSWEET11-GFP fusion protein in nodules resulted in green fluorescence associated with the plasma membrane of uninfected cells and infection thread and symbiosome membranes of infected cells. Two independent Tnt1-insertion sweet11 mutants were uncompromised in SNF Therefore, although MtSWEET11 appears to be involved in Suc distribution within nodules, it is not crucial for SNF, probably because other Suc transporters can fulfill its role(s).
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Kryvoruchko IS,Sinharoy S,Torres-Jerez I,Sosso D,Pislariu CI,Guan D,Murray J,Benedito VA,Frommer WB,Udvardi MKdoi
10.1104/pp.15.01910subject
Has Abstractpub_date
2016-05-01 00:00:00pages
554-65issue
1eissn
0032-0889issn
1532-2548pii
pp.15.01910journal_volume
171pub_type
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