Characterization of maize elongation factor 1A and its relationship to protein quality in the endosperm.

Abstract:

:The protein synthesis elongation factor 1A (eEF1A) is a multifunctional protein in eukaryotic cells. In maize (Zea mays L.) endosperm eEF1A co-localizes with actin around protein bodies, and its accumulation is highly correlated with the protein-bound lysine (Lys) content. We purified eEF1A from maize kernels by ammonium sulfate precipitation, ion-exchange, and chromatofocusing. The identify of the purified protein was confirmed by microsequencing of an endoproteinase glutamic acid-C fragment and by its ability to bundle actin. Using purified eEF1A as a standard, we found that this protein contributes 0.4% of the total protein in W64A+ endosperm and approximately 1% of the protein in W64Ao2. Because eEF1A contains 10% Lys, it accounts for 2.2% of the total Lys in W64A+ and 2.3% of the Lys in W64Ao2. However, its concentration predicts 90% of the Lys found in endosperm proteins of both genotypes, indicating that eEF1A is a key component of the group of proteins that determines the nutritional quality of the grain. This notion is further supported by the fact that in floury2, another high-Lys mutant, the content of eEF1A increases with the dosage of the floury2 gene. These data provide the biochemical basis for further investigation of the relationship between eEF1A content and the nutritional quality of cereals.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Sun Y,Carneiro N,Clore AM,Moro GL,Habben JE,Larkins BA

doi

10.1104/pp.115.3.1101

subject

Has Abstract

pub_date

1997-11-01 00:00:00

pages

1101-7

issue

3

eissn

0032-0889

issn

1532-2548

pii

115/3/1101

journal_volume

115

pub_type

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