Chloroplast thylakoid protein phosphatase is a membrane surface-associated activity.

Abstract:

:Chloroplast thylakoid protein phosphatase activity was measured using (32)P-labeled histone as an exogenous substrate and an assay of the (32)Pi released involving formation of a phosphomolybdate complex and organic extraction. The activity was liberated from wheat (Triticum aestivum) thylakoids by washing the membranes in NaCl-containing solutions followed by centrifugation. The liberated phosphatase activity had a pH optimum of approximately 6.75, was inhibited by addition of 10 millimolar EDTA or EGTA, and was stimulated by addition of millimolar amounts of dithiothreitol, magnesium, manganese, or calcium ions. The rate of thylakoid protein dephosphorylation was decreased following liberation of a portion of the protein phosphatase activity and was increased by addition of salt-liberated phosphatase fraction. These results suggest that at least a portion of wheat thylakoid protein phosphatase is a peripheral, rather than an integral, membrane protein.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Sun G,Bailey D,Jones MW,Markwell J

doi

10.1104/pp.89.1.238

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

238-43

issue

1

eissn

0032-0889

issn

1532-2548

journal_volume

89

pub_type

杂志文章
  • Actin-bundling protein isolated from pollen tubes of lily. Biochemical and immunocytochemical characterization

    abstract::A 135-kD actin-bundling protein was purified from pollen tubes of lily (Lilium longiflorum) using its affinity to F-actin. From a crude extract of the pollen tubes, this protein was coprecipitated with exogenously added F-actin and then dissociated from F-actin by treating it with high-ionic-strength solution. The pro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.4.1421

    authors: Yokota E,Shimmen KiTT

    更新日期:1998-04-01 00:00:00

  • Identification and kinetics of accumulation of proteins induced by ethylene in bean abscission zones.

    abstract::A two-dimensional gel electrophoresis system that combines a cationic polyacrylamide gel electrophoresis at pH near neutrality with sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the spectrum of basic polypeptides that accumulate in bean (Phaseolus vulgaris) abscission zones after treatm...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.98.3.955

    authors: Del Campillo E,Lewis LN

    更新日期:1992-03-01 00:00:00

  • Germanium-68 as an adequate tracer for silicon transport in plants. Characterization of silicon uptake in different crop species.

    abstract::A basic problem in silicon (Si) uptake studies in biology is the lack of an appropriate radioactive isotope. Radioactive germanium-68 ((68)Ge) has been used previously as a Si tracer in biological materials, but its suitability for the study of Si transport in higher plants is still untested. In this study, we investi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.090845

    authors: Nikolic M,Nikolic N,Liang Y,Kirkby EA,Römheld V

    更新日期:2007-01-01 00:00:00

  • Nodule-specific modulation of glutamine synthetase in transgenic Medicago truncatula leads to inverse alterations in asparagine synthetase expression.

    abstract::Transgenic Medicago truncatula plants were produced harboring chimeric gene constructs of the glutamine synthetase (GS) cDNA clones (MtGS1a or MtGS1b) fused in sense or antisense orientation to the nodule-specific leghemoglobin promoter Mtlb1. A series of transgenic plants were obtained showing a 2- to 4-fold alterati...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.017830

    authors: Carvalho HG,Lopes-Cardoso IA,Lima LM,Melo PM,Cullimore JV

    更新日期:2003-09-01 00:00:00

  • A genome-wide and metabolic analysis determined the adaptive response of Arabidopsis cells to folate depletion induced by methotrexate.

    abstract::Control of folate homeostasis is essential to sustain the demand for one-carbon (C1) units that are necessary for major biological functions, including nucleotide synthesis and methylation reactions. In this study, we analyzed the genome-wide and metabolic adaptive response of Arabidopsis (Arabidopsis thaliana) cells ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.130336

    authors: Loizeau K,De Brouwer V,Gambonnet B,Yu A,Renou JP,Van Der Straeten D,Lambert WE,Rébeillé F,Ravanel S

    更新日期:2008-12-01 00:00:00

  • incurvata13, a novel allele of AUXIN RESISTANT6, reveals a specific role for auxin and the SCF complex in Arabidopsis embryogenesis, vascular specification, and leaf flatness.

    abstract::Auxin plays a pivotal role in plant development by modulating the activity of SCF ubiquitin ligase complexes. Here, we positionally cloned Arabidopsis (Arabidopsis thaliana) incurvata13 (icu13), a mutation that causes leaf hyponasty and reduces leaf venation pattern complexity and auxin responsiveness. We found that i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.207779

    authors: Esteve-Bruna D,Pérez-Pérez JM,Ponce MR,Micol JL

    更新日期:2013-03-01 00:00:00

  • Silencing Nicotiana attenuata calcium-dependent protein kinases, CDPK4 and CDPK5, strongly up-regulates wound- and herbivory-induced jasmonic acid accumulations.

    abstract::The plant hormone jasmonic acid (JA) plays a pivotal role in plant-insect interactions. Herbivore attack usually elicits dramatic increases in JA concentrations, which in turn activate the accumulation of metabolites that function as defenses against herbivores. Although almost all enzymes involved in the biosynthesis...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.199018

    authors: Yang DH,Hettenhausen C,Baldwin IT,Wu J

    更新日期:2012-08-01 00:00:00

  • Incorporation of label into pollen tube walls from myoinositol-labeled Lilium longiflorum pistils.

    abstract::Compatible and incompatible pollen tubes growing on detached Lilium longiflorum pistils which had been prelabeled with myoinositol-U-(14)C take up a portion of the label and utilize it for biosynthesis of tube wall substance. The label is transferred from pistil to pollen tubes apparently via the secretion products (e...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.45.1.92

    authors: Kroh M,Miki-Hirosige H,Rosen W,Loewus F

    更新日期:1970-01-01 00:00:00

  • Disruption of Ethylene Responses by Turnip mosaic virus Mediates Suppression of Plant Defense against the Green Peach Aphid Vector.

    abstract::Plants employ diverse responses mediated by phytohormones to defend themselves against pathogens and herbivores. Adapted pathogens and herbivores often manipulate these responses to their benefit. Previously, we demonstrated that Turnip mosaic virus (TuMV) infection suppresses callose deposition, an important plant de...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00332

    authors: Casteel CL,De Alwis M,Bak A,Dong H,Whitham SA,Jander G

    更新日期:2015-09-01 00:00:00

  • Unique Motifs and Length of Hairpin in Oleosin Target the Cytosolic Side of Endoplasmic Reticulum and Budding Lipid Droplet.

    abstract::Plant cytosolic lipid droplets (LDs) are covered with a layer of phospholipids and oleosin and were extensively studied before those in mammals and yeast. Oleosin has short amphipathic N- and C-terminal peptides flanking a conserved 72-residue hydrophobic hairpin, which penetrates and stabilizes the LD Oleosin is synt...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00366

    authors: Huang CY,Huang AHC

    更新日期:2017-08-01 00:00:00

  • Characterization of the Proton-Translocating Cytochrome c Oxidase Activity in the Plasma Membrane of Intact Anacystis nidulans Spheroplasts.

    abstract::Intact spheroplasts of the cyanobacterium (blue-green alga) Anacystis nidulans oxidized various exogenous c-type cytochromes with concomitant outward proton translocation while exogenous ferricytochrome c was not reduced. The H(+)/e(-) stoichiometry was close to 1 with each of the cytochromes and did not depend on the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.4.968

    authors: Peschek GA

    更新日期:1984-08-01 00:00:00

  • Endogenous Bioactive Jasmonate Is Composed of a Set of (+)-7-iso-JA-Amino Acid Conjugates.

    abstract::Jasmonates (JAs) regulate a wide range of plant defense and development processes. The bioactive JA is perceived by its receptor COI1 to trigger the degradation of JASMONATE ZIM-DOMAIN (JAZ) proteins and subsequently derepress the JAZ-repressed transcription factors for activation of expression of JA-responsive genes....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00906

    authors: Yan J,Li S,Gu M,Yao R,Li Y,Chen J,Yang M,Tong J,Xiao L,Nan F,Xie D

    更新日期:2016-12-01 00:00:00

  • Mechanism of photoactivation of electron transport in intact bryopsis chloroplasts.

    abstract::The mechanism of photoactivation of photosystem I electron transport was studied in intact Bryopsis corticulans chloroplasts. The evidence from chemical and photochemical studies suggests that photoactivation is a consequence of a reduction of an electron transport component, presumably ferredoxin-NADP(+) reductase. O...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.5.1413

    authors: Satoh K

    更新日期:1982-11-01 00:00:00

  • CO(2) Enhancement of Growth and Photosynthesis in Rice (Oryza sativa) : Modification by Increased Ultraviolet-B Radiation.

    abstract::Two cultivars of rice (Oryza sativa L.) IR-36 and Fujiyama-5 were grown at ambient (360 microbars) and elevated CO(2) (660 microbars) from germination through reproduction in unshaded greenhouses at the Duke University Phytotron. Growth at elevated CO(2) resulted in significant decreases in nighttime respiration and i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.2.473

    authors: Ziska LH,Teramura AH

    更新日期:1992-06-01 00:00:00

  • Analysis of the Nicotiana tabacum stigma/style transcriptome reveals gene expression differences between wet and dry stigma species.

    abstract::The success of plant reproduction depends on pollen-pistil interactions occurring at the stigma/style. These interactions vary depending on the stigma type: wet or dry. Tobacco (Nicotiana tabacum) represents a model of wet stigma, and its stigmas/styles express genes to accomplish the appropriate functions. For a larg...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.131573

    authors: Quiapim AC,Brito MS,Bernardes LA,Dasilva I,Malavazi I,DePaoli HC,Molfetta-Machado JB,Giuliatti S,Goldman GH,Goldman MH

    更新日期:2009-03-01 00:00:00

  • Regulation of the Photosynthesis Rhythm in Euglena gracilis: I. Carbonic Anhydrase and Glyceraldehyde-3-Phosphate Dehydrogenase Do Not Regulate the Photosynthesis Rhythm.

    abstract::A circadian rhythm of O(2) evolution has been found in Euglena gracilis, Klebs strain Z. The rhythm persists for at least 5 days in constant dim light and temperature, but damps out in constant bright light. The phase of this rhythm can be shifted by a pulse of bright light and the period length is not changed over a ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.2.150

    authors: Lonergan TA,Sargent ML

    更新日期:1978-02-01 00:00:00

  • Salinity effects on photosynthesis in isolated mesophyll cells of cowpea leaves.

    abstract::Mesophyll cells from leaves of cowpea (Vigna unquiculata [L.] Walp.) plants grown under saline conditions were isolated and used for the determination of photosynthetic CO(2) fixation. Maximal CO(2) fixation rate was obtained when the osmotic potential of both cell isolation and CO(2) fixation assay media were close t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.2.493

    authors: Plaut Z,Grieve CM,Federman E

    更新日期:1989-10-01 00:00:00

  • Adaptation of the Photosynthetic Apparatus in Maize Leaves as a Result of Nitrogen Limitation : Relationships between Electron Transport and Carbon Assimilation.

    abstract::In maize (Zea mays L., cv Contessa), nitrogen (NO(3) (-)) limitation resulted in a reduction in shoot growth and photosynthetic capacity and in an increase in the leaf zeaxanthin contents. Nitrogen deficiency had only a small effect on the quantum yield of CO(2) assimilation but a large effect on the light-saturated r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.3.1436

    authors: Khamis S,Lamaze T,Lemoine Y,Foyer C

    更新日期:1990-11-01 00:00:00

  • Iron deficiency-induced secretion of phenolics facilitates the reutilization of root apoplastic iron in red clover.

    abstract::Phenolic compounds are frequently reported to be the main components of root exudates in response to iron (Fe) deficiency in Strategy I plants, but relatively little is known about their function. Here, we show that removal of secreted phenolics from the root-bathing solution almost completely inhibited the reutilizat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.095794

    authors: Jin CW,You GY,He YF,Tang C,Wu P,Zheng SJ

    更新日期:2007-05-01 00:00:00

  • Contribution of malic enzyme, pyruvate kinase, phosphoenolpyruvate carboxylase, and the krebs cycle to respiration and biosynthesis and to intracellular pH regulation during hypoxia in maize root tips observed by nuclear magnetic resonance imaging and gas

    abstract::In vivo pyruvate synthesis by malic enzyme (ME) and pyruvate kinase and in vivo malate synthesis by phosphoenolpyruvate carboxylase and the Krebs cycle were measured by 13C incorporation from [1-13C]glucose into glucose-6-phosphate, alanine, glutamate, aspartate, and malate. These metabolites were isolated from maize ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.3.1073

    authors: Edwards S,Nguyen BT,Do B,Roberts JKM

    更新日期:1998-03-01 00:00:00

  • Differential Ion Stimulation of Plasmalemma Adenosine Triphosphatase from Leaf Epidermis and Mesophyll of Nicotiana rustica L.

    abstract::An ATPase preparation, presumed to be associated with plasma membrane due to the coincidence on isopycnic gradients of cellulase and beta-glucan synthetase at high substrate, has been isolated from the epidermal and mesophyll of tobacco leaf. The ATPase from both tissues was found to prefer ATP over other nucleotides....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.5.936

    authors: Lurie S

    更新日期:1979-05-01 00:00:00

  • Characteristics of Nitrate Reductase-inactivating Proteins Obtained from Corn Roots and Rice Cell Cultures.

    abstract::Nitrate reductase (NR)-inactivating proteins from corn roots (Wf-9 x 38-11) and rice cell suspension cultures were tested against a partially purified NR obtained from corn leaves (W64A x W182E). The corn protein was purified 921-fold and the rice protein, 1,660-fold using standard purification procedures. Approximate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.1.141

    authors: Yamaya T,Oaks A,Boesel IL

    更新日期:1980-01-01 00:00:00

  • Conversion of endogenous indole-3-butyric acid to indole-3-acetic acid drives cell expansion in Arabidopsis seedlings.

    abstract::Genetic evidence in Arabidopsis (Arabidopsis thaliana) suggests that the auxin precursor indole-3-butyric acid (IBA) is converted into active indole-3-acetic acid (IAA) by peroxisomal beta-oxidation; however, direct evidence that Arabidopsis converts IBA to IAA is lacking, and the role of IBA-derived IAA is not well u...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.157461

    authors: Strader LC,Culler AH,Cohen JD,Bartel B

    更新日期:2010-08-01 00:00:00

  • Inositol polyphosphate 5-phosphatases 1 and 2 are required for regulating seedling growth.

    abstract::Signals can be perceived and amplified at the cell membrane by receptors coupled to the production of a variety of second messengers, including myoinositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)]. The myoinositol polyphosphate 5-phosphatases (5PTases; EC 3.1.3.56) comprise a large protein family that hydrolyzes 5-phospha...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.089474

    authors: Gunesekera B,Torabinejad J,Robinson J,Gillaspy GE

    更新日期:2007-03-01 00:00:00

  • Characterization of Sucrolysis via the Uridine Diphosphate and Pyrophosphate-Dependent Sucrose Synthase Pathway.

    abstract::The breakdown of sucrose to feed both hexoses into glycolytic carbon flow can occur by the sucrose synthase pathway. This uridine diphosphate (UDP) and pyrophosphate (PPi)-dependent pathway was biochemically characterized using soluble extracts from several plants. The sucrolysis process required the simultaneous pres...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.2.635

    authors: Xu DP,Sung SJ,Loboda T,Kormanik PP,Black CC

    更新日期:1989-06-01 00:00:00

  • A critical role of STAYGREEN/Mendel's I locus in controlling disease symptom development during Pseudomonas syringae pv tomato infection of Arabidopsis.

    abstract::Production of disease symptoms represents the final phase of infectious diseases and is a main cause of crop loss and/or marketability. However, little is known about the molecular basis of disease symptom development. In this study, a genetic screening was conducted to identify Arabidopsis (Arabidopsis thaliana) muta...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.181826

    authors: Mecey C,Hauck P,Trapp M,Pumplin N,Plovanich A,Yao J,He SY

    更新日期:2011-12-01 00:00:00

  • Pectin Modification in Cell Walls of Ripening Tomatoes Occurs in Distinct Domains.

    abstract::The class of cell wall polysaccharides that undergoes the most extensive modification during tomato (Lycopersicon esculentum) fruit ripening is pectin. De-esterification of the polygalacturonic acid backbone by pectin methylesterase facilitates the depolymerization of pectins by polygalacturonase II (PGII). To investi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.1.373

    authors: Steele NM,McCann MC,Roberts K

    更新日期:1997-05-01 00:00:00

  • Mechanism of Stimulation and Inhibition of Tonoplast H-ATPase of Beta vulgaris by Chloride and Nitrate.

    abstract::The H(+)-ATPase of tonoplast vesicles isolated from red beet (Beta vulgaris L.) storage tissue was studied with respect to the kinetic effects of Cl(-) and NO(3) (-). N-Ethylmaleimide (NEM) was employed as a probe to investigate substrate binding and gross conformational changes of the enzyme. Chloride decreased the K...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.81.1.120

    authors: Griffith CJ,Rea PA,Blumwald E,Poole RJ

    更新日期:1986-05-01 00:00:00

  • Comparison between a Stable NaCl-Selected Nicotiana Cell Line and the Wild Type : K, Na, and Proline Pools as a Function of Salinity.

    abstract::An NaCl-resistant line has been developed from suspension-cultured tobacco cells (Nicotiana tabacum/gossii) by stepwise increases in the NaCl concentration in the medium. Resistance showed stability through at least 24 generations in the absence of added NaCl.Above an external NaCl concentration of 35 millimolar, prol...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.3.624

    authors: Watad AE,Reinhold L,Lerner HR

    更新日期:1983-11-01 00:00:00

  • The alc-GR system: a modified alc gene switch designed for use in plant tissue culture.

    abstract::The ALCR/alcA (alc) two-component, ethanol-inducible gene expression system provides stringent control of transgene expression in genetically modified plants. ALCR is an ethanol-activated transcription factor that can drive expression from the ALCR-responsive promoter (alcA). However, the alc system has been shown to ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.059659

    authors: Roberts GR,Garoosi GA,Koroleva O,Ito M,Laufs P,Leader DJ,Caddick MX,Doonan JH,Tomsett AB

    更新日期:2005-07-01 00:00:00