Actin-bundling protein isolated from pollen tubes of lily. Biochemical and immunocytochemical characterization

Abstract:

:A 135-kD actin-bundling protein was purified from pollen tubes of lily (Lilium longiflorum) using its affinity to F-actin. From a crude extract of the pollen tubes, this protein was coprecipitated with exogenously added F-actin and then dissociated from F-actin by treating it with high-ionic-strength solution. The protein was further purified sequentially by chromatography on a hydroxylapatite column, a gel-filtration column, and a diethylaminoethyl-cellulose ion-exchange column. In the present study, this protein is tentatively referred to as P-135-ABP (Plant 135-kD Actin-Bundling Protein). By the elution position from a gel-filtration column, we estimated the native molecular mass of purified P-135-ABP to be 260 kD, indicating that it existed in a dimeric form under physiological conditions. This protein bound to and bundled F-actin prepared from chicken breast muscle in a Ca2+-independent manner. The binding of 135-P-ABP to actin was saturated at an approximate stoichiometry of 26 actin monomers to 1 dimer of P-135-ABP. By transmission electron microscopy of thin sections, we observed cross-bridges between F-actins with a longitudinal periodicity of 31 nm. Immunofluorescence microscopy using rhodamine-phalloidin and antibodies against the 135-kD polypeptide showed that P-135-ABP was colocalized with bundles of actin filaments in lily pollen tubes, leading us to conclude that it is the factor responsible for bundling the filaments.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Yokota E,Shimmen KiTT

doi

10.1104/pp.116.4.1421

subject

Has Abstract

pub_date

1998-04-01 00:00:00

pages

1421-9

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

116

pub_type

杂志文章
  • Adenosine triphosphatase from soybean callus and root cells.

    abstract::The ATPase activity of a membrane fraction from soybean (Glycine max L.) root and callus cells, presumed to be enriched in plasma membrane, has been characterized with respect to ion stimulation, pH requirement, and nucleotide specificity. The enzyme from both sources was activated by divalent cations (Mg(2+) > Mn(2+)...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.2.264

    authors: Hendrix DL,Kennedy RM

    更新日期:1977-02-01 00:00:00

  • Role of polygalacturonase in bean leaf abscission.

    abstract::The role of polygalacturonase in leaf abscission was studied in explants of Phaseolus vulgaris L. cv. Red Kidney. Bean polygalacturonase was partially characterized and comparisons were made between the bean enzyme and previously reported higher plant polygalacturonases. Polygalacturonase isolated from bean leaf absci...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.6.1133

    authors: Berger RK,Reid PD

    更新日期:1979-06-01 00:00:00

  • Effect of high temperature on calcium uptake by suspension-cultured pear fruit cells.

    abstract::Uptake of Ca(2+) by suspension-cultured pear (Pyrus communis L. cv Passe Crassane) cells and protoplasts was significantly enhanced by exposure to 38 degrees C compared to 25 degrees C. The increased uptake was specific for Ca(2+) and was not due to cell wall binding. Tissues pretreated at 38 degrees C showed increase...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.1.153

    authors: Klein JD,Ferguson IB

    更新日期:1987-05-01 00:00:00

  • Effects of drought on primary photosynthetic processes of cotton leaves.

    abstract::The effects of drought on Photosystem II (PSII) fluorescence and photosynthetic electron transport activities were analyzed in cotton. Water stress did not modify the amplitude of leaf variable fluorescence at room temperature in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) nor at 77 K. It is therefo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.2.360

    authors: Genty B,Briantais JM,Da Silva JB

    更新日期:1987-02-01 00:00:00

  • An investigation of the mineral content of barley grains and seedlings.

    abstract::The Ca, Mg, K, and P content of dry barley (Hordeum vulgare) grains and seedlings was investigated using energy dispersive x-ray analysis and neutron activation analysis. Energy dispersive x-ray analysis of protein bodies in aleurone cells showed that these bodies contained very little Ca in relation to P, Mg, and K. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.1.93

    authors: Stewart A,Nield H,Lott JN

    更新日期:1988-01-01 00:00:00

  • Enhanced copper tolerance in Silene vulgaris (Moench) Garcke populations from copper mines is associated with increased transcript levels of a 2b-type metallothionein gene.

    abstract::Silene vulgaris (Moench) Garcke has evolved populations with extremely high levels of copper tolerance. To evaluate the role of metallothioneins (MTs) in copper tolerance in S. vulgaris, we screened a cDNA library derived from a highly copper-tolerant population using Arabidopsis-based MT probes and identified an MT2b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.126.4.1519

    authors: van Hoof NA,Hassinen VH,Hakvoort HW,Ballintijn KF,Schat H,Verkleij JA,Ernst WH,Karenlampi SO,Tervahauta AI

    更新日期:2001-08-01 00:00:00

  • Adaptation of the Cyanobacterium Anabaena variabilis to Low CO(2) Concentration in Their Environment.

    abstract::The rate of adaptation of high CO(2) (5% v/v CO(2) in air)-grown Anabaena to a low level of CO(2) (0.05% v/v in air) was determined as a function of O(2) concentration. Exposure of cells to low (2.6%) O(2) concentration resulted in an extended lag in the adaptation to low CO(2) concentration. The rate of adaptation fo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.71.1.208

    authors: Marcus Y,Harel E,Kaplan A

    更新日期:1983-01-01 00:00:00

  • The regulation of gene expression in transformed maize aleurone and endosperm protoplasts. Analysis of promoter activity, intron enhancement, and mRNA untranslated regions on expression.

    abstract::Gene expression in the aleurone and endosperm is highly regulated during both seed development and germination. Studies of alpha-amylase expression in the aleurone of barley (Hordeum vulgare) have generated the current paradigm for hormonal control of gene expression in germinating cereal grain. Gene expression studie...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.3.929

    authors: Gallie DR,Young TE

    更新日期:1994-11-01 00:00:00

  • Assay of Proton-Coupled Glycolate and D-Glycerate Transport into Chloroplast Inner Envelope Membrane Vesicles by Stopped-Flow Fluorescence.

    abstract::The transport of glycolate and D-glycerate across the inner envelope membrane of intact chloroplasts is rapid and mediated by a translocator with proton/substrate symport activity. The true initial rate of glycolate or D-glycerate transport could not be measured by conventional methods. To resolve the initial rates of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.3.793

    authors: Young XK,McCarty RE

    更新日期:1993-03-01 00:00:00

  • Accumulation of soybean glycinin and its assembly with the glutelins in rice

    abstract::Saline-soluble glycinins and insoluble glutelins are the major storage proteins in soybean (Glycine max) and rice (Oryza sativa), respectively. In spite of their differences in solubility properties, both proteins are members of the 11S globulin gene family based on their similarities in primary sequences and processi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.120.4.1063

    authors: Katsube T,Kurisaka N,Ogawa M,Maruyama N,Ohtsuka R,Utsumi S,Takaiwa F

    更新日期:1999-08-01 00:00:00

  • Expression of foreign genes in transgenic yellow-poplar plants.

    abstract::Cells of yellow-poplar (Liriodendron tulipifera L.) were transformed by direct gene transfer and regenerated into plants by somatic embryogenesis. Plasmid DNA bearing marker genes encoding beta-glucuronidase (GUS) and neomycin phosphotransferase (NPT II) were introduced by microprojectile bombardment into single cells...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.98.1.114

    authors: Wilde HD,Meagher RB,Merkle SA

    更新日期:1992-01-01 00:00:00

  • Specificity of cycloheximide in higher plant systems.

    abstract::Although cycloheximide is extremely inhibitory to protein synthesis in vivo in higher plants, the reported insensitivity of some plant ribosomes suggests that it may not invariably act at the ribosomal level. This suggestion is reinforced by results obtained with red beet storage tissue disks, the respiration of which...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.46.2.227

    authors: Ellis RJ,Macdonald IR

    更新日期:1970-08-01 00:00:00

  • Characterization of glyoxysomes from castor bean endosperm.

    abstract::Electron micrographs are presented which establish the identity of the components of the 3 major bands observed after sucrose density centrifugation of the crude particulate fraction from the endosperm of germinating castor bean seedlings. These are: mitochondria (density 1.19 g/cc), proplastids (density 1.23 g/cc) an...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.5.705

    authors: Breidenbach RW,Kahn A,Beevers H

    更新日期:1968-05-01 00:00:00

  • Cytochrome c is released in a reactive oxygen species-dependent manner and is degraded via caspase-like proteases in tobacco Bright-Yellow 2 cells en route to heat shock-induced cell death.

    abstract::To gain some insight into the mechanism of plant programmed cell death, certain features of cytochrome c (cyt c) release were investigated in heat-shocked tobacco (Nicotiana tabacum) Bright-Yellow 2 cells in the 2- to 6-h time range. We found that 2 h after heat shock, cyt c is released from intact mitochondria into t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.078683

    authors: Vacca RA,Valenti D,Bobba A,Merafina RS,Passarella S,Marra E

    更新日期:2006-05-01 00:00:00

  • Adaptation to CO(2) Level and Changes in the Phosphorylation of Thylakoid Proteins during the Cell Cycle of Chlamydomonas reinhardtii.

    abstract::The photosynthetic performance of synchronously grown Chlamydomonas reinhardtii alternated rhythmically during the cell cycle. The activity of the "CO(2) concentrating mechanism" including the ability to accumulate CO(2) internally and the activity of carbonic anhydrase peaked after 6 to 9 hours of light and reached m...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.2.604

    authors: Marcus Y,Schuster G,Michaels A,Kaplan A

    更新日期:1986-02-01 00:00:00

  • Functional analysis of the cellulose synthase genes CesA1, CesA2, and CesA3 in Arabidopsis.

    abstract::Polysaccharide analyses of mutants link several of the glycosyltransferases encoded by the 10 CesA genes of Arabidopsis to cellulose synthesis. Features of those mutant phenotypes point to particular genes depositing cellulose predominantly in either primary or secondary walls. We used transformation with antisense co...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.010931

    authors: Burn JE,Hocart CH,Birch RJ,Cork AC,Williamson RE

    更新日期:2002-06-01 00:00:00

  • Green sperm. Identification of male gamete promoters in Arabidopsis.

    abstract::Previously, in an effort to better understand the male contribution to fertilization, we completed a maize (Zea mays) sperm expressed sequence tag project. Here, we used this resource to identify promoters that would direct gene expression in sperm cells. We used reverse transcription-polymerase chain reaction to iden...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.054213

    authors: Engel ML,Holmes-Davis R,McCormick S

    更新日期:2005-08-01 00:00:00

  • Clonal variation for tolerance to polyethylene glycol-induced water stress in cultured tomato cells.

    abstract::Cell clones were isolated from a population of cultured tomato (Lycopersicon esculentum Mill cv VFNT-cherry) cells and their tolerance to polyethylene glycol (PEG)-induced water stress was measured. Considerable variation for tolerance among the clones was found. Tolerance differences between clones appeared to be spo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.3.645

    authors: Handa AK,Bressan RA,Handa S,Hasegawa PM

    更新日期:1983-07-01 00:00:00

  • Cytokinins from the Moss Physcomitrella patens.

    abstract::Gametophore-over-producing mutants of the moss, Physcomitrella patens, when grown in liquid culture export high levels of cytokinin into their culture medium. The cytokinin produced by these mutants is postulated to account for their peculiar phenotype, that of mosses treated with exogenous cytokinin. N(6)-(Delta(2)-i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.3.735

    authors: Wang TL,Horgan R,Cove D

    更新日期:1981-09-01 00:00:00

  • Comparison of the Ability of Salicylic Acid and Ferricyanide to Induce Flowering in the Long-day Plant, Lemna Gibba G3.

    abstract::Both salicylic acid and ferricyanide induce flowering in the long-day plant Lemna gibba L., strain G3 under 8- and 9-hour short days. In both cases the effect is daylength-dependent. Salicylic acid is ineffective on daylengths less than 8 hours and ferricyanide is ineffective on daylengths less than 5 hours. When both...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.6.1058

    authors: Tanaka O,Cleland CF

    更新日期:1980-06-01 00:00:00

  • Diversity of the superfamily of phloem lectins (phloem protein 2) in angiosperms.

    abstract::Phloem protein 2 (PP2) is one of the most abundant and enigmatic proteins in the phloem sap. Although thought to be associated with structural P-protein, PP2 is translocated in the assimilate stream where its lectin activity or RNA-binding properties can exert effects over long distances. Analyzing the diversity of th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.013086

    authors: Dinant S,Clark AM,Zhu Y,Vilaine F,Palauqui JC,Kusiak C,Thompson GA

    更新日期:2003-01-01 00:00:00

  • Sulfur economy and cell wall biosynthesis during sulfur limitation of Chlamydomonas reinhardtii.

    abstract::We have identified two novel periplasmic/cell wall polypeptides that specifically accumulate during sulfur limitation of Chlamydomonas reinhardtii. These polypeptides, present at high levels in the extracellular polypeptide fraction from a sulfur-deprived, cell wall-minus C. reinhardtii strain, have apparent molecular...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:

    authors: Takahashi H,Braby CE,Grossman AR

    更新日期:2001-10-01 00:00:00

  • Endo-[beta]-Mannanase Activity from Individual Tomato Endosperm Caps and Radicle Tips in Relation to Germination Rates.

    abstract::Endo-[beta]-mannanase is hypothesized to be a rate-limiting enzyme in endosperm weakening, which is a prerequisite for radicle emergence from tomato (Lycopersicon esculentum Mill.) seeds. Using a sensitive, single-seed assay, we have measured mannanase activity diffusing from excised tomato endosperm caps following tr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.1.21

    authors: Still DW,Bradford KJ

    更新日期:1997-01-01 00:00:00

  • Possible involvement of calmodulin and the cytoskeleton in electrofusion of plant protoplasts.

    abstract::Calmodulin antagonists, trifluoperazine, chlorpromazine, calmidazolium, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), strongly inhibited the electrofusion of barley (Hordeum vulgare L. cv Moor) protoplasts with a marked increase of broken fusion products, after 60 minutes of incubation. W-5, a dechlorinate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.81.4.1151

    authors: Abe S,Takeda J

    更新日期:1986-08-01 00:00:00

  • DNA-binding study identifies C-box and hybrid C/G-box or C/A-box motifs as high-affinity binding sites for STF1 and LONG HYPOCOTYL5 proteins.

    abstract::LONG HYPOCOTYL5 (HY5) is a bZIP (basic leucine zipper) transcription factor that activates photomorphogenesis and root development in Arabidopsis (Arabidopsis thaliana). Previously, STF1 (soybean [Glycine max] TGACG-motif binding factor 1), a homologous legume protein with a RING-finger motif and a bZIP domain, was re...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.113217

    authors: Song YH,Yoo CM,Hong AP,Kim SH,Jeong HJ,Shin SY,Kim HJ,Yun DJ,Lim CO,Bahk JD,Lee SY,Nagao RT,Key JL,Hong JC

    更新日期:2008-04-01 00:00:00

  • Potassium Transport in Corn Roots : IV. Characterization of the Linear Component.

    abstract::A detailed examination was conducted on the linear, or first-order kinetic component for K(+)((86)Rb(+)) influx into root segments of both low- and high-salt grown corn seedlings (Zea mays [A632 x Oh 43]). In tissue from both low- and high-salt grown roots, replacement of Cl(-) in the uptake solution by either SO(4) (...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.3.771

    authors: Kochian LV,Xin-Zhi J,Lucas WJ

    更新日期:1985-11-01 00:00:00

  • Foliar sterols in soybeans exposed to chronic levels of ozone.

    abstract::Soybeans (Glycine max) exposed to chronic levels of ozone showed a linear decrease in biomass with increasing concentration. The foliar free sterols increased while the steryl ester, and the steryl glycosides, a minor component, decreased with increasing pollutant concentration. Of the free sterols, stigmasterol showe...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.1.245

    authors: Grunwald C,Endress AG

    更新日期:1985-01-01 00:00:00

  • Characterization of the Endoproteases Appearing during Wheat Grain Development.

    abstract::The pattern of endoproteolytic activities occurring during wheat (Triticum aestivum, cultivar Chinese Spring) grain development was investigated. Total endoprotease activity, assayed in solution with azocasein as a substrate, increased during the early stages of grain development to reach a maximum at 15 d postanthesi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.3.1211

    authors: Dominguez F,Cejudo FJ

    更新日期:1996-11-01 00:00:00

  • Abscisic Acid and the Photoperiodic Induction of Dormancy in Salix viminalis L.

    abstract::A series of growth room experiments was carried out aiming to establish the role of abscisic acid on dormancy of Salix viminalis L. The inhibitor content and abscisic acid levels of extracts from roots, sap, leaves, and apical tissues of willow were measured using biological assay and gas-liquid chromatography.No evid...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.4.774

    authors: Alvim R,Saunders PF,Barros RS

    更新日期:1979-04-01 00:00:00

  • Flowering responses to altered expression of phytochrome in mutants and transgenic lines of Arabidopsis thaliana (L.) Heynh.

    abstract::The long-day plant Arabidopsis thaliana (L.) Heynh. flowers early in response to brief end-of-day (EOD) exposures to far-red light (FR) following a fluorescent short day of 8 h. FR promotion of flowering was nullified by subsequent brief red light (R) EOD exposure, indicating phytochrome involvement. The EOD response ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.4.1495

    authors: Bagnall DJ,King RW,Whitelam GC,Boylan MT,Wagner D,Quail PH

    更新日期:1995-08-01 00:00:00