Characterization of the Endoproteases Appearing during Wheat Grain Development.

Abstract:

:The pattern of endoproteolytic activities occurring during wheat (Triticum aestivum, cultivar Chinese Spring) grain development was investigated. Total endoprotease activity, assayed in solution with azocasein as a substrate, increased during the early stages of grain development to reach a maximum at 15 d postanthesis that was maintained until the grain was mature. Endoprotease activity was also assayed in gradient polyacrylamide gels co-polymerized with gelatin. The increase in endoproteolytic activity was due to the appearance of up to 18 endoproteolytic bands that were arbitrarily classified into five groups (A, B, C, D, and E). The presence of serine, aspartic, metallo, and, to a lesser extent, thiol proteases in developing wheat grains was demonstrated by the use of class-specific protease inhibitors. The appearance of the different classes of endoproteases during seed development was subject to temporal control; serine proteases were more abundant at early stages and aspartic and metallo proteases were more abundant at later stages. At intermediate stages of development (15-20 d postanthesis), most of the endoproteases were localized in the aleurone, testa, and embryo. The content of acidic thiol proteases was low in the developing starchy endosperm.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Dominguez F,Cejudo FJ

doi

10.1104/pp.112.3.1211

subject

Has Abstract

pub_date

1996-11-01 00:00:00

pages

1211-1217

issue

3

eissn

0032-0889

issn

1532-2548

pii

112/3/1211

journal_volume

112

pub_type

杂志文章
  • Metabolism of D-glycero-D-manno-heptitol, volemitol, in polyanthus. Discovery Of a novel ketose reductase1

    abstract::Volemitol (D-glycero-D-manno-heptitol, alpha-sedoheptitol) is an unusual seven-carbon sugar alcohol that fulfills several important physiological functions in certain species of the genus Primula. Using the horticultural hybrid polyanthus (Primula x polyantha) as our model plant, we found that volemitol is the major n...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.119.1.191

    authors: Hafliger B,Kindhauser E,Keller F

    更新日期:1999-01-01 00:00:00

  • Isoelectric Focusing of Plant Plasma Membrane Proteins : Further Evidence that a 55 Kilodalton Polypeptide Is Associated with beta-1,3-Glucan Synthase Activity from Pea.

    abstract::Detergent-solubilized plasma membrane proteins from pea (Pisum sativum L.) stem tissue were separated by isoelectric focusing (IEF) using a Bio-Rad Rotofor cell, with the goal of identifying protein(s) involved in beta-1,3-glucan synthase (GS-II) activity. Ordinary IEF procedures result in membrane protein precipitati...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.4.1302

    authors: Dhugga KS,Ray PM

    更新日期:1991-04-01 00:00:00

  • Distinct functional properties of isoamylase-type starch debranching enzymes in monocot and dicot leaves.

    abstract::Isoamylase-type starch debranching enzymes (ISA) play important roles in starch biosynthesis in chloroplast-containing organisms, as shown by the strict conservation of both catalytically active ISA1 and the noncatalytic homolog ISA2. Functional distinctions exist between species, although they are not understood yet....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.225565

    authors: Facon M,Lin Q,Azzaz AM,Hennen-Bierwagen TA,Myers AM,Putaux JL,Roussel X,D'Hulst C,Wattebled F

    更新日期:2013-11-01 00:00:00

  • Expression and localization of nitrilase during symptom development of the clubroot disease in Arabidopsis.

    abstract::The expression of nitrilase in Arabidopsis during the development of the clubroot disease caused by the obligate biotroph Plasmodiophora brassicae was investigated. A time course study showed that only during the exponential growth phase of the clubs was nitrilase prominently enhanced in infected roots compared with c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.2.369

    authors: Grsic-Rausch S,Kobelt P,Siemens JM,Bischoff M,Ludwig-Müller J

    更新日期:2000-02-01 00:00:00

  • Acquisition of Thermotolerance in Soybean Seedlings : Synthesis and Accumulation of Heat Shock Proteins and their Cellular Localization.

    abstract::When soybean Glycine max var Wayne seedlings are shifted from a normal growth temperature of 28 degrees C up to 40 degrees C (heat shock or HS), there is a dramatic change in protein synthesis. A new set of proteins known as heat shock proteins (HSPs) is produced and normal protein synthesis is greatly reduced. A brie...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.1.152

    authors: Lin CY,Roberts JK,Key JL

    更新日期:1984-01-01 00:00:00

  • An intermediate in the synthesis of glucobrassicins from 3-indoleacetaldoxime by woad leaves.

    abstract::Leaves of woad (Isatis tinctoria L.) were found to incorporate efficiently tritiated indoleacetaldoxime and (35)S from (35)S-l-cystine into glucobrassicin and sulfoglucobrassicin. Time course of incorporation of (35)S from (35)S-cystine into the glucosinolates indicated that glucobrassicin was formed first and then su...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.1.43

    authors: Mahadevan S,Stowe BB

    更新日期:1972-07-01 00:00:00

  • Resolvase OsGEN1 Mediates DNA Repair by Homologous Recombination.

    abstract::Yen1/GEN1 are canonical Holliday junction resolvases that belong to the RAD2/XPG family. In eukaryotes, such as budding yeast, mice, worms, and humans, Yen1/GEN1 work together with Mus81-Mms4/MUS81-EME1 and Slx1-Slx4/SLX1-SLX4 in DNA repair by homologous recombination to maintain genome stability. In plants, the biolo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01726

    authors: Wang C,Higgins JD,He Y,Lu P,Zhang D,Liang W

    更新日期:2017-02-01 00:00:00

  • Chromosomes carrying meiotic avoidance loci in three apomictic eudicot Hieracium subgenus Pilosella species share structural features with two monocot apomicts.

    abstract::The LOSS OF APOMEIOSIS (LOA) locus is one of two dominant loci known to control apomixis in the eudicot Hieracium praealtum. LOA stimulates the differentiation of somatic aposporous initial cells after the initiation of meiosis in ovules. Aposporous initial cells undergo nuclear proliferation close to sexual megaspore...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.181164

    authors: Okada T,Ito K,Johnson SD,Oelkers K,Suzuki G,Houben A,Mukai Y,Koltunow AM

    更新日期:2011-11-01 00:00:00

  • Protein Synthesis in Imbibed Seeds III. Kinetics of Amino Acid Incorporation Ribosome Activation, and Polysome Formation.

    abstract::The kinetics of development of protein-synthesizing capacity in the imbibing wheat embryo, were studied both in vivo and in vitro. During the first 30 minutes of imbibition protein-synthesizing capacity rises rapidly, lagging about 10 minutes behind water uptake. This rise in synthesizing capacity is accompanied by an...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.7.1167

    authors: Marcus A,Feeley J,Volcani T

    更新日期:1966-09-01 00:00:00

  • Fractionation of the three stable oxygen isotopes by oxygen-producing and oxygen-consuming reactions in photosynthetic organisms.

    abstract::The triple isotope composition (delta17O and delta18O) of dissolved O2 in the ocean and in ice cores was recently used to assess the primary productivity over broad spatial and temporal scales. However, assessment of the productivity with the aid of this method must rely on accurate measurements of the 17O/16O versus ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.063768

    authors: Helman Y,Barkan E,Eisenstadt D,Luz B,Kaplan A

    更新日期:2005-08-01 00:00:00

  • Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei.

    abstract::We describe a simple and highly effective means for global identification of genes that are expressed within specific cell types within complex tissues. It involves transgenic expression of nuclear-targeted green fluorescent protein in a cell-type-specific manner. The fluorescent nuclei are then purified from homogena...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.115246

    authors: Zhang C,Barthelson RA,Lambert GM,Galbraith DW

    更新日期:2008-05-01 00:00:00

  • C(6)-[benzene ring]-indole-3-acetic Acid: a new internal standard for quantitative mass spectral analysis of indole-3-acetic Acid in plants.

    abstract::Indole-3-acetic acid (IAA) labeled with (13)C in the six carbons of the benzene ring is described for use as an internal standard for quantitative mass spectral analysis of IAA by gas chromatography/selected ion monitoring. [(13)C(6)]IAA was compared to the available deuterium labeled compounds and shown to offer the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.1.14

    authors: Cohen JD,Baldi BG,Slovin JP

    更新日期:1986-01-01 00:00:00

  • Anaerobiosis induces transcription but not translation of sucrose synthase in maize.

    abstract::This report examines the effect of anaerobic stress on the expression of sucrose synthase in maize (Zea mays L.). Following 24 hours of anaerobic treatment, alcohol dehydrogenase displayed the classical characteristics of induction: increased mRNA and protein levels. However, there was no detectable increase in sucros...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.2.542

    authors: McElfresh KC,Chourey PS

    更新日期:1988-06-01 00:00:00

  • Activation of a Reserve Pool of Photosystem II in Chlamydomonas reinhardtii Counteracts Photoinhibition.

    abstract::The effect of strong irradiance (2000 micromole photons per square meter per second) on PSII heterogeneity in intact cells of Chlamydomonas reinhardtii was investigated. Low light (LL, 15 micromole photons per square meter per second) grown C. reinhardtii are photoinhibited upon exposure to strong irradiance, and the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.92.4.1196

    authors: Neale PJ,Melis A

    更新日期:1990-04-01 00:00:00

  • Endogenous gibberellin and abscisic Acid content as related to senescence of detached lettuce leaves.

    abstract::Levels of gibberillins (GAs) and of abscisic acid (ABA) in attached leaves of romaine lettuce (Lactuca sativa L.) declined as the leaf became older. The time course of changes in hormone levels, determined in detached lettuce leaves kept in darkness, revealed that a sharp decline in GAs accompanied by a moderate rise ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.2.224

    authors: Aharoni N

    更新日期:1978-08-01 00:00:00

  • Auxin Conjugation by Tobacco Mesophyll Protoplasts : Correlations between Auxin Cytotoxicity under Low Density Growth Conditions and Induction of Conjugation Processes at High Density.

    abstract::Auxin induction of the proliferation of Nicotiana tabacum (cv Xanthi) mesophyll protoplasts and of protoplast-derived cells was studied. The growth-promoting properties and cytotoxicities at high concentrations of IAA and naphthaleneacetic acid were strongly affected by cell density. The induction of growth by 2,4-dic...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.1.54

    authors: Caboche M,Aranda G,Poll AM,Huet JC,Leguay JJ

    更新日期:1984-05-01 00:00:00

  • Vacuolar/Extravacuolar Distribution of Aminopeptidases in Giant Alga Chara australis and Partial Purification of One Such Enzyme.

    abstract::The presence of two major aminopeptidases (aminopeptidases I and II) in the giant alga Chara australis was shown using polyacrylamide gel electrophoresis. Partially purified aminopeptidase I had a molecular weight of about 120,000, hydrolyzed both leucine-beta-naphthylamide (pH optimum 6.0) and alanine-beta-naphthylam...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.3.720

    authors: Moriyasu Y,Sakano K,Tazawa M

    更新日期:1987-07-01 00:00:00

  • The UAP56-Interacting Export Factors UIEF1 and UIEF2 Function in mRNA Export.

    abstract::In eukaryotes, the regulated transport of mRNAs from the nucleus to the cytosol through nuclear pore complexes represents an important step in the expression of protein-coding genes. In plants, the mechanism of nucleocytosolic mRNA transport and the factors involved are poorly understood. The Arabidopsis (Arabidopsis ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.01476

    authors: Ehrnsberger HF,Pfaff C,Hachani I,Flores-Tornero M,Sørensen BB,Längst G,Sprunck S,Grasser M,Grasser KD

    更新日期:2019-04-01 00:00:00

  • Light-induced growth promotion by SPA1 counteracts phytochrome-mediated growth inhibition during de-etiolation.

    abstract::Previous evidence has suggested that SPA1 is a signal transduction component that appears to require phytochrome A for function in seedling photomorphogenesis. Using digital image analysis, we examined the time course of growth inhibition induced by red light in spa1 mutants to test the interpretation that SPA1 functi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.126.3.1291

    authors: Parks BM,Hoecker U,Spalding EP

    更新日期:2001-07-01 00:00:00

  • Functional classification, genomic organization, putatively cis-acting regulatory elements, and relationship to quantitative trait loci, of sorghum genes with rhizome-enriched expression.

    abstract::Rhizomes are organs of fundamental importance to plant competitiveness and invasiveness. We have identified genes expressed at substantially higher levels in rhizomes than other plant parts, and explored their functional categorization, genomic organization, regulatory motifs, and association with quantitative trait l...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.082891

    authors: Jang CS,Kamps TL,Skinner DN,Schulze SR,Vencill WK,Paterson AH

    更新日期:2006-11-01 00:00:00

  • Fatty acid activation in cyanobacteria mediated by acyl-acyl carrier protein synthetase enables fatty acid recycling.

    abstract::In cyanobacteria fatty acids destined for lipid synthesis can be synthesized de novo, but also exogenous free fatty acids from the culture medium can be directly incorporated into lipids. Activation of exogenous fatty acids is likely required prior to their utilization. To identify the enzymatic activity responsible f...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.148007

    authors: Kaczmarzyk D,Fulda M

    更新日期:2010-03-01 00:00:00

  • Further Characterization on the Transport Property of Plasmalemma NADH Oxidation System in Isolated Corn Root Protoplasts.

    abstract::Recent experiments show that exogenous NADH increases the O(2) consumption and uptake of inorganic ions into isolated corn (Zea mays L. Pioneer Hybrid 3320) root protoplasts (Lin 1982, Proc Natl Acad Sci USA 79: 3773-3776). A mild treatment of protoplasts with trypsin released most of the NADH oxidation system from th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.2.219

    authors: Lin W

    更新日期:1984-02-01 00:00:00

  • Partial Purification and Characterization of d-Ribose-5-phosphate Reductase from Adonis vernalis L. Leaves.

    abstract::This study presents evidence for a new enzyme, d-ribose-5-P reductase, which catalyzes the reaction: d-ribose-5-P + NADPH + H(+) --> d-ribitol-5-P + NADP(+). The enzyme was isolated from Adonis vernalis L. leaves in 38% yield and was purified 71-fold. The reductase was NADPH specific and had a pH optimum in the range ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.4.758

    authors: Negm FB,Marlow GC

    更新日期:1985-08-01 00:00:00

  • Developmentally Regulated Expression of the Gene Family for Cytosolic Glutamine Synthetase in Pisum sativum.

    abstract::In Pisum sativum, two classes of genes encode distinct isoforms of cytosolic glutamine synthetase (GS). The first class comprises two nearly identical or "twin" GS genes (GS341 and GS132), while the second comprises a single GS gene (GS299) distinct in both coding and noncoding regions from the "twin" GS genes. Gene-s...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.2.702

    authors: Walker EL,Coruzzi GM

    更新日期:1989-10-01 00:00:00

  • Fifty years of progress in water relations research.

    abstract::Many of the basic concepts dealing with soil and plant water relationships were in existence 50 years ago, but were inadequately presented in the textbooks of that time. There has been a marked increase in the amount of work done in this field during recent decades, but much of it involves advances in understanding th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.4.463

    authors: Kramer PJ

    更新日期:1974-10-01 00:00:00

  • Photosynthesis in Fescue : III. RATES OF ELECTRON TRANSPORT IN A POLYPLOID SERIES OF TALL FESCUE PLANTS.

    abstract::Photosystem I electron transport activity has been found to be considerably higher in a decaploid tall fescue (Festuca arundinacea Schreb.) genotype as compared to a common hexaploid genotype. The decaploid genotype also displayed a higher photosystem whole chain (Photosystem II plus Photosystem I uncoupled) activity,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.5.1110

    authors: Krueger RW,Miles D

    更新日期:1981-11-01 00:00:00

  • Partial purification and characterization of the mitochondrial and peroxisomal isozymes of enoyl-coenzyme a hydratase from germinating pea seedlings.

    abstract::Distinct organellar forms of the beta-oxidation enzyme enoyl-coenzyme A (CoA) hydratase were partially purified and characterized from 2-day germinated pea (Pisum sativum L.) seedlings. The purification was accomplished by disruption of purified mitochondria or peroxisomes, (NH(4))(2)SO(4) fractionation, and gel perme...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.2.564

    authors: Miernyk JA,Thomas DR,Wood C

    更新日期:1991-02-01 00:00:00

  • Starch-synthesizing Enzymes in the Endosperm and Pollen of Maize.

    abstract::Two mutations, amylose-extender and waxy, which affect the proportion of amylose and amylopectin of starch synthesized in the endosperm of maize (Zea mays L.) seeds, are also expressed in the pollen. However, most mutations that affect starch synthesis in the maize endosperm are not expressed in the pollen. In an atte...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.2.312

    authors: Bryce WH,Nelson OE

    更新日期:1979-02-01 00:00:00

  • Protein and RNA Synthesizing Activities during Sexual Process of Heterothallic Closterium.

    abstract::Protein and RNA synthesizing activities increased markedly during the mating process and decreased during the maturation stage of zygotes in heterothallic strains of Closterium peracerosum-strigosum-littoale, KAS-4-29 (mating-type minus) and KAS -4-30 (mating-type plus) and a homothallic Closterium acerosum. Different...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.4.696

    authors: Kato A,Sasaki K

    更新日期:1985-08-01 00:00:00

  • Characterization of a meta-Fluorotyrosine-Tolerant Cell Culture of Eschscholtzia californica Cham.

    abstract::A cell line of Eschscholtzia californica selected for meta-fluorotyrosine (MFT) tolerance was found to have 10-fold increased levels of phenylalanine and tyrosine compared to the parent line, while most other amino acids were only increased 2-fold. Tracer experiments with shikimic acid in the presence of MFT showed th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.4.1299

    authors: Berlin J,van Huystee RB,Witte L,Bender J,Jäger HJ,Wray V

    更新日期:1989-04-01 00:00:00