Flowering responses to altered expression of phytochrome in mutants and transgenic lines of Arabidopsis thaliana (L.) Heynh.


:The long-day plant Arabidopsis thaliana (L.) Heynh. flowers early in response to brief end-of-day (EOD) exposures to far-red light (FR) following a fluorescent short day of 8 h. FR promotion of flowering was nullified by subsequent brief red light (R) EOD exposure, indicating phytochrome involvement. The EOD response to R or FR is a robust measure of phytochrome action. Along with their wild-type (WT) parents, mutants deficient in either phytochrome A or B responded similarly to the EOD treatments. Thus, neither phytochrome A nor B exclusively regulated flowering, although phytochrome B controlled hypocotyl elongation. Perhaps a third phytochrome species is important for the EOD responses of the mutants and/or their flowering is regulated by the amount of the FR-absorbing form of phytochrome, irrespective of the phytochrome species. Overexpression of phytochrome A or phytochrome B resulted in differing photoperiod and EOD responses among the genotypes. The day-neutral overexpressor of phytochrome A had an EOD response similar to all of the mutants and WTs, whereas R EOD exposure promoted flowering in the overexpressor of phytochrome B and FR EOD exposure inhibited this promotion. The comparisons between relative flowering times and leaf numbers at flowering of the over-expressors and their WTs were not consistent across photoperiods and light treatments, although both phytochromes A and B contributed to regulating flowering of the transgenic plants.


Plant Physiol


Plant physiology


Bagnall DJ,King RW,Whitelam GC,Boylan MT,Wagner D,Quail PH




Has Abstract


1995-08-01 00:00:00














  • Light-induced Changes of the Carotenoid Levels in Chloroplast Envelopes.

    abstract::The carotenoid content of thylakoids and envelopes isolated from dark-or light-treated spinach (Spinacia oleracea L.) chloroplasts was compared. In thylakoids, light induced a decrease of violaxanthin parallel with a stoichiometric increase of zeaxanthin due to violaxanthin deepoxidation. In envelopes, violaxanthin wa...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Siefermann-Harms D

    更新日期:1978-04-01 00:00:00

  • Nitrogen Use Efficiency Is Mediated by Vacuolar Nitrate Sequestration Capacity in Roots of Brassica napus.

    abstract::Enhancing nitrogen use efficiency (NUE) in crop plants is an important breeding target to reduce excessive use of chemical fertilizers, with substantial benefits to farmers and the environment. In Arabidopsis (Arabidopsis thaliana), allocation of more NO3 (-) to shoots was associated with higher NUE; however, the comm...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Han YL,Song HX,Liao Q,Yu Y,Jian SF,Lepo JE,Liu Q,Rong XM,Tian C,Zeng J,Guan CY,Ismail AM,Zhang ZH

    更新日期:2016-03-01 00:00:00

  • Isolation and Characterization of the Multiple 7S Globulins of Soybean Proteins.

    abstract::Two major proteins (the 7S and 11S globulins) of soybean (Glycine max) were simultaneously isolated by a simple method based on their different solubilities in dilute tris (hydroxymethyl) aminomethane buffers. The purified 7S globulins, which represented essentially the entire 7S soybean protein fraction capable of di...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Thanh VH,Okubo K,Shibasaki K

    更新日期:1975-07-01 00:00:00

  • Growth, Gravitropism, and Endogenous Ion Currents of Cress Roots (Lepidium sativum L.) : Measurements Using a Novel Three-Dimensional Recording Probe.

    abstract::A novel, three-dimensional recording, vibrating probe was used for measuring the density and direction of the endogenous ionic current of cress roots (Lepidium sativum L.) bathed in low salt media (artificial pond water, APW). Roots submerged in regular APW and growing vertically show the following current pattern. Cu...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Weisenseel MH,Becker HF,Ehlgötz JG

    更新日期:1992-09-01 00:00:00

  • Phloem Transport of Amino Acids in Relation to their Cytosolic Levels in Barley Leaves.

    abstract::A comparison of barley (Hordeum vulgare L.) leaves was made between the cytosolic content of amino acids and sucrose as determined by subcellular fractionation and the corresponding concentration in phloem sap, which was collected continuously for up to 6 days from severed aphid stylets. Because amino acids were found...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Winter H,Lohaus G,Heldt HW

    更新日期:1992-07-01 00:00:00

  • Purification and Characteristics of Sorbitol-6-phosphate Dehydrogenase from Loquat Leaves.

    abstract::To study the role of sorbitol-6-phosphate dehydrogenase in sorbitol synthesis in leaves of Rosaceous plants, properties of the enzyme and its presence in several plants in the family was investigated. The activity of the enzyme, which catalyzes an NADP-dependent oxidation of the substrate to glucose-6-phosphate, was d...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Hirai M

    更新日期:1981-02-01 00:00:00

  • Indole acetic acid distribution coincides with vascular differentiation pattern during Arabidopsis leaf ontogeny.

    abstract::We used an anti-indole acetic acid (IAA or auxin) monoclonal antibody-based immunocytochemical procedure to monitor IAA level in Arabidopsis tissues. Using immunocytochemistry and the IAA-driven beta-glucuronidase (GUS) activity of Aux/IAA promoter::GUS constructs to detect IAA distribution, we investigated the role o...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Avsian-Kretchmer O,Cheng JC,Chen L,Moctezuma E,Sung ZR

    更新日期:2002-09-01 00:00:00

  • Increased ratio of electron transport to net assimilation rate supports elevated isoprenoid emission rate in eucalypts under drought.

    abstract::Plants undergoing heat and low-CO2 stresses emit large amounts of volatile isoprenoids compared with those in stress-free conditions. One hypothesis posits that the balance between reducing power availability and its use in carbon assimilation determines constitutive isoprenoid emission rates in plants and potentially...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Dani KG,Jamie IM,Prentice IC,Atwell BJ

    更新日期:2014-10-01 00:00:00

  • Cytokinin-to-Auxin Ratios and Morphology of Shoots and Tissues Transformed by a Chimeric Isopentenyl Transferase Gene.

    abstract::Tissues transformed with the isopentenyl transferase (ipt) gene cloned from the T-DNA region of the Ti plasmid or with the ipt gene placed under the control of the cauliflower mosaic virus 35S promoter (35S-ipt) were analyzed for auxin and cytokinin. Free and total indole-3-acetic acid (IAA) levels in 35S-ipt-transfor...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Smigocki AC,Owens LD

    更新日期:1989-11-01 00:00:00

  • Induced beta-carotene synthesis driven by triacylglycerol deposition in the unicellular alga dunaliella bardawil

    abstract::Under stress conditions such as high light intensity or nutrient starvation, cells of the unicellular alga Dunaliella bardawil overproduce beta-carotene, which is accumulated in the plastids in newly formed triacylglycerol droplets. We report here that the formation of these sequestering structures and beta-carotene a...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Rabbani S,Beyer P,Lintig J,Hugueney P,Kleinig H

    更新日期:1998-04-01 00:00:00

  • Purification and Characterization of Microsomal Cytochrome b(5) and NADH Cytochrome b(5) Reductase from Pisum sativum.

    abstract::In this communication we document the reproducible protocols for the purification of milligram quantities of cytochrome b(5) and NADH-cytochrome b(5) reductase from the microsomal fraction of Pisum sativum. The cytochrome b(5) component of this NADH linked electron transport chain was found to have a molecular mass of...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Jollie DR,Sligar SG,Schuler M

    更新日期:1987-10-01 00:00:00

  • Glycoprotein Synthesis in Plants: II. Structure of the Mannolipid Intermediate.

    abstract::This paper reports the purification and structural determination of the mannolipid shown previously (Ericson and Delmer 1977 Plant Physiol 59: 341-347) to serve as an intermediate in glycoprotein synthesis in cotyledons of Phaseolus vulgaris. The mannolipid was purified by chromatography in organic solvents on diethyl...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Delmer DP,Kulow C,Ericson MC

    更新日期:1978-01-01 00:00:00

  • Sugar Potentiation of Fatty Acid and Triacylglycerol Accumulation.

    abstract::Photosynthetically derived sugar provides carbon skeletons for lipid biosynthesis. We used mutants of Arabidopsis (Arabidopsis thaliana) and the expression of oleogenic factors to investigate relationships among sugar availability, lipid synthesis, and the accumulation of triacylglycerol (TAG) in leaf tissue. The adg1...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Zhai Z,Liu H,Xu C,Shanklin J

    更新日期:2017-10-01 00:00:00

  • The Respiratory Chain of Plant Mitochondria: XIV. Ordering of Ubiquinone, Flavoproteins, and Cytochromes in the Respiratory Chain.

    abstract::The effect of initial oxygen concentration on the rate and extent of oxidation of the respiratory chain carriers of anaerobic mitochondria from mung bean (Phaseolus aureus) seedlings was examined. The substrate was succinate, with malonate added to give malonate to succinate ratios of 6 to 12, thereby minimizing the f...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Storey BT,Bahr JT

    更新日期:1972-07-01 00:00:00

  • Molecular analysis of two cDNA clones encoding acidic class I chitinase in maize.

    abstract::The cloning and analysis of two different cDNA clones encoding putative maize (Zea mays L.) chitinases obtained by polymerase chain reaction (PCR) and cDNA library screening is described. The cDNA library was made from poly(A)+ RNA from leaves challenged with mercuric chloride for 2 d. The two clones, pCh2 and pCh11, ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Wu S,Kriz AL,Widholm JM

    更新日期:1994-08-01 00:00:00

  • Mutations in UDP-Glucose:sterol glucosyltransferase in Arabidopsis cause transparent testa phenotype and suberization defect in seeds.

    abstract::In higher plants, the most abundant sterol derivatives are steryl glycosides (SGs) and acyl SGs. Arabidopsis (Arabidopsis thaliana) contains two genes, UGT80A2 and UGT80B1, that encode UDP-Glc:sterol glycosyltransferases, enzymes that catalyze the synthesis of SGs. Lines having mutations in UGT80A2, UGT80B1, or both U...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: DeBolt S,Scheible WR,Schrick K,Auer M,Beisson F,Bischoff V,Bouvier-Navé P,Carroll A,Hematy K,Li Y,Milne J,Nair M,Schaller H,Zemla M,Somerville C

    更新日期:2009-09-01 00:00:00

  • Comparing the in vivo function of α-carboxysomes and β-carboxysomes in two model cyanobacteria.

    abstract::The carbon dioxide (CO2)-concentrating mechanism of cyanobacteria is characterized by the occurrence of Rubisco-containing microcompartments called carboxysomes within cells. The encapsulation of Rubisco allows for high-CO2 concentrations at the site of fixation, providing an advantage in low-CO2 environments. Cyanoba...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Whitehead L,Long BM,Price GD,Badger MR

    更新日期:2014-05-01 00:00:00

  • Roles of BOR2, a boron exporter, in cross linking of rhamnogalacturonan II and root elongation under boron limitation in Arabidopsis.

    abstract::Boron (B) is required for cross linking of the pectic polysaccharide rhamnogalacturonan II (RG-II) and is consequently essential for the maintenance of cell wall structure. Arabidopsis (Arabidopsis thaliana) BOR1 is an efflux B transporter for xylem loading of B. Here, we describe the roles of BOR2, the most similar p...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Miwa K,Wakuta S,Takada S,Ide K,Takano J,Naito S,Omori H,Matsunaga T,Fujiwara T

    更新日期:2013-12-01 00:00:00

  • Interference in Carotenogenesis as a Mechanism of Action of the Pyridazinone Herbicide Sandoz 6706: Accumulation of C-40 Carotenoid Precursors Inhibition of beta-Carotene Synthesis and Enhancement of Phytoene Epoxidation.

    abstract::The herbicide Sandoz 6706 (4-chloro-5-(dimethylamino)-2-alpha,alpha,alpha, (trifluoro-m-tolyl)-3(2H)-pyridazinone), when applied as a preplant soil treatment at a concentration of 0.05 mug/g reduced the content of beta-carotene and chlorophylls in 21-day-old wheat seedlings (Triticum aestivum L.) by 55% and 29%, respe...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Ben-Aziz A,Koren E

    更新日期:1974-12-01 00:00:00

  • An Improved Brome mosaic virus Silencing Vector: Greater Insert Stability and More Extensive VIGS.

    abstract::Virus-induced gene silencing (VIGS) is used extensively for gene function studies in plants. VIGS is inexpensive and rapid compared with silencing conducted through stable transformation, but many virus-silencing vectors, especially in grasses, induce only transient silencing phenotypes. A major reason for transient p...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Ding XS,Mannas SW,Bishop BA,Rao X,Lecoultre M,Kwon S,Nelson RS

    更新日期:2018-01-01 00:00:00

  • The last step in cocaine biosynthesis is catalyzed by a BAHD acyltransferase.

    abstract::The esterification of methylecgonine (2-carbomethoxy-3β-tropine) with benzoic acid is the final step in the biosynthetic pathway leading to the production of cocaine in Erythoxylum coca. Here we report the identification of a member of the BAHD family of plant acyltransferases as cocaine synthase. The enzyme is capabl...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Schmidt GW,Jirschitzka J,Porta T,Reichelt M,Luck K,Torre JC,Dolke F,Varesio E,Hopfgartner G,Gershenzon J,D'Auria JC

    更新日期:2015-01-01 00:00:00

  • Phytoalexin Elicitor Activity of Carbohydrates from Phytophthora megasperma f.sp. glycinea and Other Sources.

    abstract::Three unique classes of carbohydrates were isolated from the hyphal cell walls of Phytophthora megasperma f.sp. glycinea (Pmg) and compared with other substances for their activity as elicitors of the phytoalexin glyceollin in soybean tissues. Glucomannans extracted from cell walls with soybean beta-1,3-endoglucanase ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Keen NT,Yoshikawa M,Wang MC

    更新日期:1983-03-01 00:00:00

  • Isolation and characterization of cDNAs expressed in the early stages of flavonol-induced pollen germination in petunia.

    abstract::Petunia (Petunia hybrida) pollen requires flavonols (Fl) to germinate. Adding kaempferol to Fl-deficient pollen causes rapid and synchronous germination and tube outgrowth. We exploited this system to identify genes responsive to Fls and to examine the changes in gene expression that occur during the first 0.5 h of po...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Guyon VN,Astwood JD,Garner EC,Dunker AK,Taylor LP

    更新日期:2000-06-01 00:00:00

  • Acclimation of Photosynthetic Light Reactions during Induction of Inorganic Carbon Accumulation in the Green Alga Chlamydomonas reinhardtii.

    abstract::Cells of the unicellular green algae Chlamydomonas reinhardtii were grown in high dissolved inorganic carbon (DIC) concentrations (supplied with 50 milliliters per liter CO(2)[g]) and transferred to low DIC concentrations (supplied with

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Palmqvist K,Sundblad LG,Wingsle G,Samuelsson G

    更新日期:1990-09-01 00:00:00

  • High genetic variability of herbivore-induced volatile emission within a broad range of maize inbred lines.

    abstract::Maize plants (Zea mays) attacked by caterpillars release a mixture of odorous compounds that attract parasitic wasps, natural enemies of the herbivores. We assessed the genetic variability of these induced volatile emissions among 31 maize inbred lines representing a broad range of genetic diversity used by breeders i...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Degen T,Dillmann C,Marion-Poll F,Turlings TC

    更新日期:2004-08-01 00:00:00

  • Role of polygalacturonase in bean leaf abscission.

    abstract::The role of polygalacturonase in leaf abscission was studied in explants of Phaseolus vulgaris L. cv. Red Kidney. Bean polygalacturonase was partially characterized and comparisons were made between the bean enzyme and previously reported higher plant polygalacturonases. Polygalacturonase isolated from bean leaf absci...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Berger RK,Reid PD

    更新日期:1979-06-01 00:00:00

  • Transformation of Soybean Cells Using Mixed Strains of Agrobacterium tumefaciens and Phenolic Compounds.

    abstract::Cotyledon explants from germinated 1-day-old soybean seedling were inoculated with single or mixed strains of Agrobacterium tumefaciens. Mixed-strain infections with the supervirulent L,L-succinamopine type strain A281 (pTiBo542) and strain LBA4404 carrying an octopine type virulence (vir) region and a binary vector (...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Owens LD,Smigocki AC

    更新日期:1988-11-01 00:00:00

  • Mutational analysis of branching in pea. Evidence that Rms1 and Rms5 regulate the same novel signal.

    abstract::The fifth increased branching ramosus (rms) mutant, rms5, from pea (Pisum sativum), is described here for phenotype and grafting responses with four other rms mutants. Xylem sap zeatin riboside concentration and shoot auxin levels in rms5 plants have also been compared with rms1 and wild type (WT). Rms1 and Rms5 appea...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Morris SE,Turnbull CG,Murfet IC,Beveridge CA

    更新日期:2001-07-01 00:00:00

  • Radioautographic analysis of the distribution of label from h-indoleacetic Acid supplied to isolated coleus internodes.

    abstract::Isolated fifth internodes of Coleus blumei Benth. were supplied with (3)H-IAA at their apical ends. Microradioautography, using thin sections (0.25-0.5 microns) of Epon embedded tissue and liquid emulsion techniques, revealed extremely specific localization of the acetone-insoluble derivatives of IAA. Changes in the p...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Sabnis DD,Hirshberg G,Jacobs WP

    更新日期:1969-01-01 00:00:00

  • A dominant mutation in the light-oxygen and voltage2 domain vicinity impairs phototropin1 signaling in tomato.

    abstract::In higher plants, blue light (BL) phototropism is primarily controlled by the phototropins, which are also involved in stomatal movement and chloroplast relocation. These photoresponses are mediated by two phototropins, phot1 and phot2. Phot1 mediates responses with higher sensitivity than phot2, and phot2 specificall...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Sharma S,Kharshiing E,Srinivas A,Zikihara K,Tokutomi S,Nagatani A,Fukayama H,Bodanapu R,Behera RK,Sreelakshmi Y,Sharma R

    更新日期:2014-04-01 00:00:00