Accumulation of soybean glycinin and its assembly with the glutelins in rice

Abstract:

:Saline-soluble glycinins and insoluble glutelins are the major storage proteins in soybean (Glycine max) and rice (Oryza sativa), respectively. In spite of their differences in solubility properties, both proteins are members of the 11S globulin gene family based on their similarities in primary sequences and processing of the coded protein. Wild-type and methionine-modified glycinin coding sequences were expressed in transgenic rice plants under the control of the rice glutelin GluB-1 promoter. Glycinins were specifically synthesized in the endosperm tissue and co-localized with glutelins in type II protein bodies. They assembled into 7S and 11S species, similar to what was observed in developing soybean seeds. This pattern was quite different from that displayed by the rice glutelins in untransformed plants, in which processed subunits sedimenting at 2S were apparent. In glycinin-expressing transgenic plants, however, glutelins were observed sedimenting at 7S and 11S with lesser amounts in the 2S region. A portion of the glycinins was also found associated in the insoluble glutelin fraction. Renaturation experiments suggested that the hybrid glycinin-glutelin oligomers were formed through specific interactions. Overall, these results indicate that despite significant differences in the assembly of soybean glycinin and rice glutelin, both proteins can assemble with each other to form soluble hexameric oligomers or insoluble aggregates.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Katsube T,Kurisaka N,Ogawa M,Maruyama N,Ohtsuka R,Utsumi S,Takaiwa F

doi

10.1104/pp.120.4.1063

subject

Has Abstract

pub_date

1999-08-01 00:00:00

pages

1063-74

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

120

pub_type

杂志文章
  • Isolation of chloroplastic phosphoglycerate kinase : kinetics of the two-enzyme phosphoglycerate kinase/glyceraldehyde-3-phosphate dehydrogenase couple.

    abstract::We report here a method for the isolation of high specific activity phosphoglycerate kinase (EC 2.7.2.3) from chloroplasts. The enzyme has been purified over 200-fold from pea (Pisum sativum L.) stromal extracts to apparent homogeneity with 23% recovery. Negative cooperativity is observed with the two enzyme phosphogl...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.1.291

    authors: Macioszek J,Anderson JB,Anderson LE

    更新日期:1990-09-01 00:00:00

  • Biochemical and molecular inhibition of plastidial carbonic anhydrase reduces the incorporation of acetate into lipids in cotton embryos and tobacco cell suspensions and leaves.

    abstract::Two cDNAs encoding functional carbonic anhydrase (CA) enzymes were recently isolated from a non-photosynthetic, cotyledon library of cotton (Gossypium hirsutum) seedlings with putative plastid-targeting sequences (GenBank accession nos. AF132854 and AF132855). Relative CA transcript abundance and enzyme activity incre...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.010879

    authors: Hoang CV,Chapman KD

    更新日期:2002-04-01 00:00:00

  • Isolation of a plant glycoprotein involved with control of intercellular recognition.

    abstract::A recognition molecule was isolated from stigmas of S-allele genotype S(2)S(2) of Brassica oleracea var. capitata L. After Sephadex chromatography, it eluted as a single symmetrical peak during diethylaminoethane-cellulose chromatography. A high degree of purity was affirmed by: sedimentation as a single peak during u...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.67.2.270

    authors: Ferrari TE,Bruns D,Wallace DH

    更新日期:1981-02-01 00:00:00

  • Water Stress Effects on Nitrogen Assimilation and Growth of Trifolium subterraneum L. Using Dinitrogen or Ammonium Nitrate.

    abstract::The relative effects of water stress on growth parameters of subterranean clover (Trifolium subterraneum L. cv. Woogenellup) dependent on either N(2) or 8 millimolar NH(4)NO(3) for N were examined. Whole-plant carbon exchange rate (CER), acetylene reduction (AR), dry matter production, and Kjeldahl N accumulation were...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.2.416

    authors: Dejong TM,Phillips DA

    更新日期:1982-02-01 00:00:00

  • Compartmentation in Vicia faba Leaves: I. Kinetics of C in the Tissues following Pulse Labeling.

    abstract::Leaflets of Vicia faba were pulse-labeled with (14)CO(2) to follow the subsequent movement of photosynthate between leaf tissues. Samples were taken during a (12)CO(2) chase, quick frozen, freeze-substituted, and embedded in methacrylate. Paradermal sections provided tissue samples consisting only of upper epidermis, ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.4.699

    authors: Outlaw WH,Fisher DB

    更新日期:1975-04-01 00:00:00

  • Acclimation to Fluctuating Light Impacts the Rapidity of Response and Diurnal Rhythm of Stomatal Conductance.

    abstract::Plant acclimation to growth light environment has been studied extensively; however, the majority of these studies have focused on light intensity and photo-acclimation, with few studies exploring the impact of dynamic growth light on stomatal acclimation and behavior. To assess the impact of growth light regime on st...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.01809

    authors: Matthews JSA,Vialet-Chabrand S,Lawson T

    更新日期:2018-03-01 00:00:00

  • New lv Mutants of Pea Are Deficient in Phytochrome B.

    abstract::The lv-1 mutant of pea (Pisum sativum L.) is deficient in responses regulated by phytochrome B (phyB) in other species but has normal levels of spectrally active phyB. We have characterized three further lv mutants (lv-2, lv-3, and lv-4), which are all elongated under red (R) and white light but are indistinguishable ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.2.525

    authors: Weller JL,Nagatani A,Kendrick RE,Murfet IC,Reid JB

    更新日期:1995-06-01 00:00:00

  • The occurrence of phytoferritin and its relationship to effectiveness of soybean nodules.

    abstract::Polyacrylamide gel electrophoresis and electron microscopy revealed that accumulation of iron-protein in soybean nodules is influenced by nodule age, mutation in bradyrhizobia, and rhizobial/bradyrhizobial strain-soybean cultivar interactions. Iron-protein concentrations (micrograms per milligram protein) were inverse...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.2.299

    authors: Ko MP,Huang PY,Huang JS,Barker KR

    更新日期:1987-02-01 00:00:00

  • Evidence for osmotic regulation of hydrolytic enzyme production in germinating barley seeds.

    abstract::alpha-Amylase levels in intact seeds of barley (Hordeum vulgare L. cv. Himalaya) reach a maximum at 3 to 4 days of germination while gibberellin levels continue to increase beyond 6 days of germination. In contrast to its effect on half seeds, gibberellic acid does not increase the total amount of alpha-amylase produc...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.48.2.137

    authors: Jones RL,Armstrong JE

    更新日期:1971-08-01 00:00:00

  • Characterization of the Destruction of Phytochrome in the Red-absorbing Form.

    abstract::Both the red-absorbing (Pr) and far red-absorbing (Pfr) forms of phytochrome undergo destruction, defined as the loss of photoreversibly detectable chromoprotein following actinic irradiation of dark-grown tissue, in 4-day-old etiolated oat seedlings. Pr and Pfr destruction follow the same time course, exhibit the sam...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.4.680

    authors: Stone HJ,Pratt LH

    更新日期:1979-04-01 00:00:00

  • Role of Ethylene in the Geotropic Response of Bermudagrass (Cynodon dactylon L. Pers.) Stolons.

    abstract::We studied the relationship between ethylene and gravity-induced upward bending of bermudagrass (Cynodon dactylon L. Pers.) stolons. Ethylene production begins within 3 hours of the onset of gravistimulation, and increases thereafter until the 15th hour, after which it declines. There is a close positive relationship ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.4.1251

    authors: Balatti PA,Willemöes JG

    更新日期:1989-12-01 00:00:00

  • Transport of sodium into the xylem exudate of tobacco.

    abstract::When tobacco (Nicotiana tabacum L. var. Virginia Gold) plants were pretreated with Na ((22)Na) several days before detopping, from 2.3 to 4.9% of Na previously accumulated in roots appeared in the xylem exudate in 7 days after detopping. Na from the external medium, however, was readily transported to the exudate. Mor...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.3.388

    authors: Wallace A,Abou-Zamzam AM,Mueller RT

    更新日期:1972-09-01 00:00:00

  • Carbon Oxysulfide Inhibition of the CO(2)-Concentrating Process of Unicellular Green Algae.

    abstract::Carbonyl sulfide (COS), a substrate for carbonic anhydrase, inhibited alkalization of the medium, O(2) evolution, dissolved inorganic carbon accumulation, and photosynthetic CO(2) fixation at pH 7 or higher by five species of unicellular green algae that had been air-adapted for forming a CO(2)-concentrating process. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.98.2.578

    authors: Goyal A,Shiraiwa Y,Tolbert NE

    更新日期:1992-02-01 00:00:00

  • CO(2) Assimilation by Etiolated Hordeum vulgare Seedlings during the Onset of Photosynthesis.

    abstract::The assimilation of CO(2) by etiolated Hordeum vulgare seedlings during an illumination period indicates a conversion of the organisms to autotrophy.After 1 hour illumination, increases in the photo-assimilation of CO(2) are observed and the distribution of C(14) in the soluble fraction of the plants is predominantly ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.1.115

    authors: Biggins J,Park RB

    更新日期:1966-01-01 00:00:00

  • Electrostatic Changes in Lycopersicon esculentum Root Plasma Membrane Resulting from Salt Stress.

    abstract::Salinity-induced alterations in tomato (Lypersicon esculentum Mill. cv Heinz 1350) root plasma membrane properties were studied and characterized using a membrane vesicle system. Equivalent rates of MgATP-dependent H(+)-transport activity were measured by quinacrine fluorescence (DeltapH) in plasma membrane vesicles i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.2.471

    authors: Suhayda CG,Giannini JL,Briskin DP,Shannon MC

    更新日期:1990-06-01 00:00:00

  • Characterization of a Diffusible Signal Capable of Inducing Defense Gene Expression in Tobacco.

    abstract::Treatment of tobacco (Nicotiana tabacum) cell-suspension cultures with cryptogein, an elicitin protein from Phytophthora cryptogea, resulted in the release of a factor(s) that diffused through a 1000-D cutoff dialysis membrane and was capable of inducing sesquiterpene cyclase enzyme activity (a key phytoalexin biosynt...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.2.621

    authors: Chappell J,Levine A,Tenhaken R,Lusso M,Lamb C

    更新日期:1997-02-01 00:00:00

  • Expression of foreign genes in transgenic yellow-poplar plants.

    abstract::Cells of yellow-poplar (Liriodendron tulipifera L.) were transformed by direct gene transfer and regenerated into plants by somatic embryogenesis. Plasmid DNA bearing marker genes encoding beta-glucuronidase (GUS) and neomycin phosphotransferase (NPT II) were introduced by microprojectile bombardment into single cells...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.98.1.114

    authors: Wilde HD,Meagher RB,Merkle SA

    更新日期:1992-01-01 00:00:00

  • Role of roots in regulating the growth rate and cytokinin content in leaves.

    abstract::The role of roots in the enhancement of cytokinin content and leaf growth of Phaseolus vulgaris plants after decapitation and partial defoliation was investigated. Partial excision of the roots of plants which were decapitated above the primary leaf node resulted in a reduction of leaf growth and soluble proteins accu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.1.76

    authors: Carmi A,Staden JV

    更新日期:1983-09-01 00:00:00

  • Reaction Properties of the Ascorbic Acid Oxidase from Myrothecium verrucaria.

    abstract::Ascorbic acid oxidase activity in Myrothecium verrucaria extracts resulted in O(2) uptake exceeding 0.5 mole per mole of ascorbic acid and in CO(2) evolution. Measurement of oxidized ascorbic acid at completion of the reaction demonstrated that an average of 10% of the oxidized product disappeared. A comparison of the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.10.1553

    authors: Lillehoj EB,Smith FG

    更新日期:1966-12-01 00:00:00

  • Nod factors induce nod factor cleaving enzymes in pea roots. Genetic and pharmacological approaches indicate different activation mechanisms.

    abstract::Establishment of symbiosis between legumes and rhizobia requires bacterial Nod factors (NFs). The concentration of these lipochitooligosaccharides in the rhizosphere is influenced by plant enzymes. NFs induce on pea (Pisum sativum) a particular extracellular NF hydrolase that releases lipodisaccharides from NFs from S...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.061705

    authors: Ovtsyna AO,Dolgikh EA,Kilanova AS,Tsyganov VE,Borisov AY,Tikhonovich IA,Staehelin C

    更新日期:2005-10-01 00:00:00

  • Aluminum resistance in the Arabidopsis mutant alr-104 is caused by an aluminum-induced increase in rhizosphere pH.

    abstract::A mechanism that confers increased Al resistance in the Arabidopsis thaliana mutant alr-104 was investigated. A modified vibrating microelectrode system was used to measure H+ fluxes generated along the surface of small Arabidopsis roots. In the absence of Al, no differences in root H+ fluxes between wild type and alr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.1.19

    authors: Degenhardt J,Larsen PB,Howell SH,Kochian LV

    更新日期:1998-05-01 00:00:00

  • Specificity of hydrolysis of phytic acid by alkaline phytase from lily pollen.

    abstract::Phytases are the primary enzymes responsible for the hydrolysis of phytic acid, myo-inositol-1,2,3,4,5,6-hexakisphosphate (I-1,2,3,4,5,6-P6). A number of phytases with varying specificities, properties, and localizations hydrolyze phytic acid present in cells. The specificity of hydrolysis of phytic acid by alkaline p...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.4.1489

    authors: Barrientos L,Scott JJ,Murthy PP

    更新日期:1994-12-01 00:00:00

  • Short and long term effects of root and shoot chilling of ransom soybean.

    abstract::The immediate short term effects on some physiological processes and the long term effects on morphology and reproductive development of root- and shoot-chilled soybeans (Glycine max L. cv Ransom) were studied. Roots or shoots of 16- or 17-day-old plants were chilled at 10 degrees C for one week, and then rewarmed to ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.3.778

    authors: Musser RL,Thomas SA,Kramer PJ

    更新日期:1983-11-01 00:00:00

  • Specific degradation of a plant leucyl transfer ribonucleic Acid by a factor in the homologous synthetase preparation.

    abstract::Partially purified aminoacyl synthetase preparations from pea roots (Pisum sativum L. var. Alaska) contain a heat-labile factor which can degrade leucyl-tRNA(6) (leu) to a new species. The singular electrophoretic and chromatographic mobilities, the isoprenoid nucleoside content, and the charging characteristics of th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.52.3.292

    authors: Babcock DF,Morris RO

    更新日期:1973-09-01 00:00:00

  • Gene Expression in Developing Wheat Endosperm : Accumulation of Gliadin and ADPglucose Pyrophosphorylase Messenger RNAs and Polypeptides.

    abstract::The developmental accumulation pattern of messenger RNA transcripts and polypeptides for wheat gliadins and ADPglucose pyrophosphorylase was determined using cDNA and antibody probes. Gliadin mRNA was detected on Northern and RNA dot blots at 3 days after flowering, it increased 100-fold by 10 days and decreased subse...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.1.34

    authors: Reeves CD,Krishnan HB,Okita TW

    更新日期:1986-09-01 00:00:00

  • Rearrangement of actin microfilaments in plant root hairs responding to rhizobium etli nodulation signals

    abstract::The response of the actin cytoskeleton to nodulation (Nod) factors secreted by Rhizobium etli has been studied in living root hairs of bean (Phaseolus vulgaris) that were microinjected with fluorescein isothiocyanate-phalloidin. In untreated control cells or cells treated with the inactive chitin oligomer, the actin c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.3.871

    authors: Crdenas L,Vidali L,Domnguez J,Prez H,Snchez F,Hepler PK,Quinto C

    更新日期:1998-03-01 00:00:00

  • Pollen Tube Discharge Completes the Process of Synergid Degeneration That Is Initiated by Pollen Tube-Synergid Interaction in Arabidopsis.

    abstract::In flowering plant reproduction, pollen tube reception is the signaling system that results in pollen tube discharge, synergid degeneration, and successful delivery of male gametes (two sperm cells) to the site where they can fuse with female gametes (egg cell and central cell). Some molecules required for this comple...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00528

    authors: Leydon AR,Tsukamoto T,Dunatunga D,Qin Y,Johnson MA,Palanivelu R

    更新日期:2015-09-01 00:00:00

  • Regulation of Nicotine Biosynthesis by an Endogenous Target Mimicry of MicroRNA in Tobacco.

    abstract::The interaction between noncoding endogenous target mimicry (eTM) and its corresponding microRNA (miRNA) is a newly discovered regulatory mechanism and plays pivotal roles in various biological processes in plants. Tobacco (Nicotiana tabacum) is a model plant for studying secondary metabolite alkaloids, of which nicot...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00649

    authors: Li F,Wang W,Zhao N,Xiao B,Cao P,Wu X,Ye C,Shen E,Qiu J,Zhu QH,Xie J,Zhou X,Fan L

    更新日期:2015-10-01 00:00:00

  • Transcriptome Association Identifies Regulators of Wheat Spike Architecture.

    abstract::The architecture of wheat (Triticum aestivum) inflorescence and its complexity is among the most important agronomic traits that influence yield. For example, wheat spikes vary considerably in the number of spikelets, which are specialized reproductive branches, and the number of florets, which are spikelet branches t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00694

    authors: Wang Y,Yu H,Tian C,Sajjad M,Gao C,Tong Y,Wang X,Jiao Y

    更新日期:2017-10-01 00:00:00

  • fhy3-1 retains inductive responses of phytochrome A.

    abstract::The fhy3 mutation of Arabidopsis impairs phytochrome A (phyA)-mediated inhibition of hypocotyl growth without affecting the levels of phyA measured spectrophotometrically or immunochemically. We investigated whether the fhy3-1 mutation has similar effects on very low fluence responses (VLFR) and high irradiance respon...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.123.1.235

    authors: Yanovsky MJ,Whitelam GC,Casal JJ

    更新日期:2000-05-01 00:00:00