Abstract:
:Saline-soluble glycinins and insoluble glutelins are the major storage proteins in soybean (Glycine max) and rice (Oryza sativa), respectively. In spite of their differences in solubility properties, both proteins are members of the 11S globulin gene family based on their similarities in primary sequences and processing of the coded protein. Wild-type and methionine-modified glycinin coding sequences were expressed in transgenic rice plants under the control of the rice glutelin GluB-1 promoter. Glycinins were specifically synthesized in the endosperm tissue and co-localized with glutelins in type II protein bodies. They assembled into 7S and 11S species, similar to what was observed in developing soybean seeds. This pattern was quite different from that displayed by the rice glutelins in untransformed plants, in which processed subunits sedimenting at 2S were apparent. In glycinin-expressing transgenic plants, however, glutelins were observed sedimenting at 7S and 11S with lesser amounts in the 2S region. A portion of the glycinins was also found associated in the insoluble glutelin fraction. Renaturation experiments suggested that the hybrid glycinin-glutelin oligomers were formed through specific interactions. Overall, these results indicate that despite significant differences in the assembly of soybean glycinin and rice glutelin, both proteins can assemble with each other to form soluble hexameric oligomers or insoluble aggregates.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Katsube T,Kurisaka N,Ogawa M,Maruyama N,Ohtsuka R,Utsumi S,Takaiwa Fdoi
10.1104/pp.120.4.1063subject
Has Abstractpub_date
1999-08-01 00:00:00pages
1063-74issue
4eissn
0032-0889issn
1532-2548journal_volume
120pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::We report here a method for the isolation of high specific activity phosphoglycerate kinase (EC 2.7.2.3) from chloroplasts. The enzyme has been purified over 200-fold from pea (Pisum sativum L.) stromal extracts to apparent homogeneity with 23% recovery. Negative cooperativity is observed with the two enzyme phosphogl...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.1.291
更新日期:1990-09-01 00:00:00
abstract::Two cDNAs encoding functional carbonic anhydrase (CA) enzymes were recently isolated from a non-photosynthetic, cotyledon library of cotton (Gossypium hirsutum) seedlings with putative plastid-targeting sequences (GenBank accession nos. AF132854 and AF132855). Relative CA transcript abundance and enzyme activity incre...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010879
更新日期:2002-04-01 00:00:00
abstract::A recognition molecule was isolated from stigmas of S-allele genotype S(2)S(2) of Brassica oleracea var. capitata L. After Sephadex chromatography, it eluted as a single symmetrical peak during diethylaminoethane-cellulose chromatography. A high degree of purity was affirmed by: sedimentation as a single peak during u...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.2.270
更新日期:1981-02-01 00:00:00
abstract::The relative effects of water stress on growth parameters of subterranean clover (Trifolium subterraneum L. cv. Woogenellup) dependent on either N(2) or 8 millimolar NH(4)NO(3) for N were examined. Whole-plant carbon exchange rate (CER), acetylene reduction (AR), dry matter production, and Kjeldahl N accumulation were...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.2.416
更新日期:1982-02-01 00:00:00
abstract::Leaflets of Vicia faba were pulse-labeled with (14)CO(2) to follow the subsequent movement of photosynthate between leaf tissues. Samples were taken during a (12)CO(2) chase, quick frozen, freeze-substituted, and embedded in methacrylate. Paradermal sections provided tissue samples consisting only of upper epidermis, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.55.4.699
更新日期:1975-04-01 00:00:00
abstract::Plant acclimation to growth light environment has been studied extensively; however, the majority of these studies have focused on light intensity and photo-acclimation, with few studies exploring the impact of dynamic growth light on stomatal acclimation and behavior. To assess the impact of growth light regime on st...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.01809
更新日期:2018-03-01 00:00:00
abstract::The lv-1 mutant of pea (Pisum sativum L.) is deficient in responses regulated by phytochrome B (phyB) in other species but has normal levels of spectrally active phyB. We have characterized three further lv mutants (lv-2, lv-3, and lv-4), which are all elongated under red (R) and white light but are indistinguishable ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.2.525
更新日期:1995-06-01 00:00:00
abstract::Polyacrylamide gel electrophoresis and electron microscopy revealed that accumulation of iron-protein in soybean nodules is influenced by nodule age, mutation in bradyrhizobia, and rhizobial/bradyrhizobial strain-soybean cultivar interactions. Iron-protein concentrations (micrograms per milligram protein) were inverse...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.83.2.299
更新日期:1987-02-01 00:00:00
abstract::alpha-Amylase levels in intact seeds of barley (Hordeum vulgare L. cv. Himalaya) reach a maximum at 3 to 4 days of germination while gibberellin levels continue to increase beyond 6 days of germination. In contrast to its effect on half seeds, gibberellic acid does not increase the total amount of alpha-amylase produc...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.48.2.137
更新日期:1971-08-01 00:00:00
abstract::Both the red-absorbing (Pr) and far red-absorbing (Pfr) forms of phytochrome undergo destruction, defined as the loss of photoreversibly detectable chromoprotein following actinic irradiation of dark-grown tissue, in 4-day-old etiolated oat seedlings. Pr and Pfr destruction follow the same time course, exhibit the sam...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.4.680
更新日期:1979-04-01 00:00:00
abstract::We studied the relationship between ethylene and gravity-induced upward bending of bermudagrass (Cynodon dactylon L. Pers.) stolons. Ethylene production begins within 3 hours of the onset of gravistimulation, and increases thereafter until the 15th hour, after which it declines. There is a close positive relationship ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.91.4.1251
更新日期:1989-12-01 00:00:00
abstract::When tobacco (Nicotiana tabacum L. var. Virginia Gold) plants were pretreated with Na ((22)Na) several days before detopping, from 2.3 to 4.9% of Na previously accumulated in roots appeared in the xylem exudate in 7 days after detopping. Na from the external medium, however, was readily transported to the exudate. Mor...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.50.3.388
更新日期:1972-09-01 00:00:00
abstract::Carbonyl sulfide (COS), a substrate for carbonic anhydrase, inhibited alkalization of the medium, O(2) evolution, dissolved inorganic carbon accumulation, and photosynthetic CO(2) fixation at pH 7 or higher by five species of unicellular green algae that had been air-adapted for forming a CO(2)-concentrating process. ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.2.578
更新日期:1992-02-01 00:00:00
abstract::The assimilation of CO(2) by etiolated Hordeum vulgare seedlings during an illumination period indicates a conversion of the organisms to autotrophy.After 1 hour illumination, increases in the photo-assimilation of CO(2) are observed and the distribution of C(14) in the soluble fraction of the plants is predominantly ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.1.115
更新日期:1966-01-01 00:00:00
abstract::Salinity-induced alterations in tomato (Lypersicon esculentum Mill. cv Heinz 1350) root plasma membrane properties were studied and characterized using a membrane vesicle system. Equivalent rates of MgATP-dependent H(+)-transport activity were measured by quinacrine fluorescence (DeltapH) in plasma membrane vesicles i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.2.471
更新日期:1990-06-01 00:00:00
abstract::Treatment of tobacco (Nicotiana tabacum) cell-suspension cultures with cryptogein, an elicitin protein from Phytophthora cryptogea, resulted in the release of a factor(s) that diffused through a 1000-D cutoff dialysis membrane and was capable of inducing sesquiterpene cyclase enzyme activity (a key phytoalexin biosynt...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.2.621
更新日期:1997-02-01 00:00:00
abstract::Cells of yellow-poplar (Liriodendron tulipifera L.) were transformed by direct gene transfer and regenerated into plants by somatic embryogenesis. Plasmid DNA bearing marker genes encoding beta-glucuronidase (GUS) and neomycin phosphotransferase (NPT II) were introduced by microprojectile bombardment into single cells...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.1.114
更新日期:1992-01-01 00:00:00
abstract::The role of roots in the enhancement of cytokinin content and leaf growth of Phaseolus vulgaris plants after decapitation and partial defoliation was investigated. Partial excision of the roots of plants which were decapitated above the primary leaf node resulted in a reduction of leaf growth and soluble proteins accu...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.73.1.76
更新日期:1983-09-01 00:00:00
abstract::Ascorbic acid oxidase activity in Myrothecium verrucaria extracts resulted in O(2) uptake exceeding 0.5 mole per mole of ascorbic acid and in CO(2) evolution. Measurement of oxidized ascorbic acid at completion of the reaction demonstrated that an average of 10% of the oxidized product disappeared. A comparison of the...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.10.1553
更新日期:1966-12-01 00:00:00
abstract::Establishment of symbiosis between legumes and rhizobia requires bacterial Nod factors (NFs). The concentration of these lipochitooligosaccharides in the rhizosphere is influenced by plant enzymes. NFs induce on pea (Pisum sativum) a particular extracellular NF hydrolase that releases lipodisaccharides from NFs from S...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.061705
更新日期:2005-10-01 00:00:00
abstract::A mechanism that confers increased Al resistance in the Arabidopsis thaliana mutant alr-104 was investigated. A modified vibrating microelectrode system was used to measure H+ fluxes generated along the surface of small Arabidopsis roots. In the absence of Al, no differences in root H+ fluxes between wild type and alr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.117.1.19
更新日期:1998-05-01 00:00:00
abstract::Phytases are the primary enzymes responsible for the hydrolysis of phytic acid, myo-inositol-1,2,3,4,5,6-hexakisphosphate (I-1,2,3,4,5,6-P6). A number of phytases with varying specificities, properties, and localizations hydrolyze phytic acid present in cells. The specificity of hydrolysis of phytic acid by alkaline p...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.4.1489
更新日期:1994-12-01 00:00:00
abstract::The immediate short term effects on some physiological processes and the long term effects on morphology and reproductive development of root- and shoot-chilled soybeans (Glycine max L. cv Ransom) were studied. Roots or shoots of 16- or 17-day-old plants were chilled at 10 degrees C for one week, and then rewarmed to ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.73.3.778
更新日期:1983-11-01 00:00:00
abstract::Partially purified aminoacyl synthetase preparations from pea roots (Pisum sativum L. var. Alaska) contain a heat-labile factor which can degrade leucyl-tRNA(6) (leu) to a new species. The singular electrophoretic and chromatographic mobilities, the isoprenoid nucleoside content, and the charging characteristics of th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.52.3.292
更新日期:1973-09-01 00:00:00
abstract::The developmental accumulation pattern of messenger RNA transcripts and polypeptides for wheat gliadins and ADPglucose pyrophosphorylase was determined using cDNA and antibody probes. Gliadin mRNA was detected on Northern and RNA dot blots at 3 days after flowering, it increased 100-fold by 10 days and decreased subse...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.82.1.34
更新日期:1986-09-01 00:00:00
abstract::The response of the actin cytoskeleton to nodulation (Nod) factors secreted by Rhizobium etli has been studied in living root hairs of bean (Phaseolus vulgaris) that were microinjected with fluorescein isothiocyanate-phalloidin. In untreated control cells or cells treated with the inactive chitin oligomer, the actin c...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.116.3.871
更新日期:1998-03-01 00:00:00
abstract::In flowering plant reproduction, pollen tube reception is the signaling system that results in pollen tube discharge, synergid degeneration, and successful delivery of male gametes (two sperm cells) to the site where they can fuse with female gametes (egg cell and central cell). Some molecules required for this comple...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00528
更新日期:2015-09-01 00:00:00
abstract::The interaction between noncoding endogenous target mimicry (eTM) and its corresponding microRNA (miRNA) is a newly discovered regulatory mechanism and plays pivotal roles in various biological processes in plants. Tobacco (Nicotiana tabacum) is a model plant for studying secondary metabolite alkaloids, of which nicot...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00649
更新日期:2015-10-01 00:00:00
abstract::The architecture of wheat (Triticum aestivum) inflorescence and its complexity is among the most important agronomic traits that influence yield. For example, wheat spikes vary considerably in the number of spikelets, which are specialized reproductive branches, and the number of florets, which are spikelet branches t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00694
更新日期:2017-10-01 00:00:00
abstract::The fhy3 mutation of Arabidopsis impairs phytochrome A (phyA)-mediated inhibition of hypocotyl growth without affecting the levels of phyA measured spectrophotometrically or immunochemically. We investigated whether the fhy3-1 mutation has similar effects on very low fluence responses (VLFR) and high irradiance respon...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.123.1.235
更新日期:2000-05-01 00:00:00