Characterization of the Destruction of Phytochrome in the Red-absorbing Form.

Abstract:

:Both the red-absorbing (Pr) and far red-absorbing (Pfr) forms of phytochrome undergo destruction, defined as the loss of photoreversibly detectable chromoprotein following actinic irradiation of dark-grown tissue, in 4-day-old etiolated oat seedlings. Pr and Pfr destruction follow the same time course, exhibit the same time delay after actinic irradiation when the plants are grown in sealed containers, result in a loss of antigenically detectable phytochrome, as determined by radial immunodiffusion assay, equal to the loss of spectrophotometrically detectable phytochrome, and have the same sensitivity to 2-mercaptoethanol and azide. We suggest that Pr destruction is a consequence of the same mechanism that is responsible for Pfr destruction.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Stone HJ,Pratt LH

doi

10.1104/pp.63.4.680

subject

Has Abstract

pub_date

1979-04-01 00:00:00

pages

680-2

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

63

pub_type

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