Abstract:
:Preliminary results from differential centrifugation experiments, washing treatments, and enrichment in linear sucrose gradients at a density of 1.09 grams per cubic centimeter all indicated that beta-glucosidase activity in corn root homogenates was associated with a membrane such as tonoplast. A subsequent sucrose density gradient centrifugation time course showed that the beta-glucosidase was actually a soluble enzyme which moved into the gradients. The problem of soluble enzymes contaminating light density membranes in sucrose gradients and the question of centrifugation time necessary for membrane vesicles to reach isopycnic conditions are addressed.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Nagahashi G,Baker AFdoi
10.1104/pp.76.4.861subject
Has Abstractpub_date
1984-12-01 00:00:00pages
861-4issue
4eissn
0032-0889issn
1532-2548journal_volume
76pub_type
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