beta-Glucosidase Activity in Corn Roots: Problems in Subcellular Fractionation.

Abstract:

:Preliminary results from differential centrifugation experiments, washing treatments, and enrichment in linear sucrose gradients at a density of 1.09 grams per cubic centimeter all indicated that beta-glucosidase activity in corn root homogenates was associated with a membrane such as tonoplast. A subsequent sucrose density gradient centrifugation time course showed that the beta-glucosidase was actually a soluble enzyme which moved into the gradients. The problem of soluble enzymes contaminating light density membranes in sucrose gradients and the question of centrifugation time necessary for membrane vesicles to reach isopycnic conditions are addressed.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Nagahashi G,Baker AF

doi

10.1104/pp.76.4.861

subject

Has Abstract

pub_date

1984-12-01 00:00:00

pages

861-4

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

76

pub_type

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