A Starch Deficient Mutant of Arabidopsis thaliana with Low ADPglucose Pyrophosphorylase Activity Lacks One of the Two Subunits of the Enzyme.

Abstract:

:A starch deficient mutant of Arabidopsis thaliana (L.) Heynh. has been isolated in which leaf extracts contain only about 5% as much activity of ADPglucose pyrophosphorylase (EC 2.7.7.27) as the wild type. A single, nuclear mutation at a previously undescribed locus designated adg2 is responsible for the mutant phenotype. Although the mutant contained only 5% as much ADPglucose pyrophosphorylase activity as the wild type, it accumulated 40% as much starch when grown in a 12 hour photoperiod. The mutant also contained about 40% as much starch as the wild type when grown in continuous light, suggesting that the rate of synthesis regulates its steady state accumulation. Immunological analysis of leaf extracts using antibodies against the spinach 54 and 51 kilodalton (kD) ADPglucose pyrophosphorylase subunits indicated that the mutant is deficient in a cross-reactive 54 kD polypeptide and has only about 4% as much as the wild type of a cross-reactive 51 kD polypeptide. This result and genetic studies suggested that adg2 is a structural gene which codes for the 54 kD polypeptide, and provides the first functional evidence that the 54 kD polypeptide is a required component of the native ADPglucose pyrophosphorylase enzyme.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Lin TP,Caspar T,Somerville CR,Preiss J

doi

10.1104/pp.88.4.1175

subject

Has Abstract

pub_date

1988-12-01 00:00:00

pages

1175-81

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

88

pub_type

杂志文章
  • Grain Protein Accumulation and the Relationship between Leaf Nitrate Reductase and Protease Activities during Grain Development in Maize (Zea mays L.): I. VARIATION BETWEEN GENOTYPES.

    abstract::Four maize hybrids, two with high and two with low levels of postanthesis nitrate reductase activity were grown under field conditions. The characteristic enzyme patterns had been established in previous work. Nitrate reductase and proteases were measured in three representative leaves (ear leaf, fourth leaf above and...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.1.164

    authors: Reed AJ,Below FE,Hageman RH

    更新日期:1980-07-01 00:00:00

  • Photoinactivation of Detergent-Solubilized Plasma Membrane ATPase from Rosa damascena: Action Spectra.

    abstract::The photochemistry of vesicular and detergent-solubilized preparations of plasma membrane-associated ATPase was investigated in Rosa damascena. The cholate-solubilized ATPase activity fractionated into two peaks on a Sephadex G-150 column with simple, but different ultraviolet (UV) sensitivities. The larger enzyme was...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.3.617

    authors: Imbrie CW,Murphy TM

    更新日期:1984-03-01 00:00:00

  • Purification, characterization, and structural analysis of a plant low-temperature-induced protein.

    abstract::We have purified to near homogeneity a recombinant form of the protein BN28 (rBN28), expressed in response to low temperature in Brassica napus plants, and we have determined its solution structure. Antibodies raised against rBN28 were used to characterize the recombinant and native proteins. Similar to many other low...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.2.367

    authors: Boothe JG,Sönnichsen FD,de Beus MD,Johnson-Flanagan AM

    更新日期:1997-02-01 00:00:00

  • Sensitivity to an Ethylene Biosynthesis-Inducing Endoxylanase in Nicotiana tabacum L. cv Xanthi Is Controlled by a Single Dominant Gene.

    abstract::The ethylene biosynthesis-inducing xylanase (EIX) is known to be a potent elicitor of ethylene biosynthesis and other responses when applied to leaf tissue of Nicotiana tabacum L. cv Xanthi. In contrast, leaf tissue of the tobacco cultivar Hicks was insensitive to EIX at concentrations 100-fold higher than was needed ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.3.1081

    authors: Bailey BA,Korcak RF,Anderson JD

    更新日期:1993-03-01 00:00:00

  • SHORT-ROOT and SCARECROW regulate leaf growth in Arabidopsis by stimulating S-phase progression of the cell cycle.

    abstract::SHORT-ROOT (SHR) and SCARECROW (SCR) are required for stem cell maintenance in the Arabidopsis (Arabidopsis thaliana) root meristem, ensuring its indeterminate growth. Mutation of SHR and SCR genes results in disorganization of the quiescent center and loss of stem cell activity, resulting in the cessation of root gro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.158857

    authors: Dhondt S,Coppens F,De Winter F,Swarup K,Merks RM,Inzé D,Bennett MJ,Beemster GT

    更新日期:2010-11-01 00:00:00

  • Inhibition of Flowering of Xanthium pensylvanicum Wallr. by Prolonged Irradiation with Far Red.

    abstract::Interrupting each long night with a prolonged period of far red radiant energy resulted in the inhibition of floral initiation in cocklebur. Irradiations inducing different relative levels of P(FR) from 1 to 2% to 80% had about the same effect under 4-hour photoperiods. The lower levels of P(FR) induced by continuous ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.1.95

    authors: Mancinelli AL,Downs RJ

    更新日期:1967-01-01 00:00:00

  • Altered nitrogenous pools induced by the azolla-anabaena azolla symbiosis.

    abstract::The free amino acid and ammonia pools of Azolla caroliniana were analyzed by quantitative column chromatography on columns capable of separating all of the nitrogenous constituents normally found in physiological fluids. Comparisons were made of plants containing symbiotic algae and grown on nitrogen-free media, plant...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.6.798

    authors: Newton JW,Cavins JF

    更新日期:1976-12-01 00:00:00

  • Ozone-Induced Alterations in the Accumulation of Newly Synthesized Proteins in Leaves of Maize.

    abstract::We examined the response of leaves of 3-week-old maize (Zea mays L.) to short-term (5 h) fumigation with O3-enriched air (0, 0.12, 0.24, or 0.36 [mu]L/L). Older leaves and leaf tissue developed more severe visible damage at higher external O3 concentrations. To investigate the immediate effect of O3 exposure on the ac...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.2.777

    authors: Pino ME,Mudd JB,Bailey-Serres J

    更新日期:1995-06-01 00:00:00

  • Chloroplast Membrane Remodeling during Freezing Stress Is Accompanied by Cytoplasmic Acidification Activating SENSITIVE TO FREEZING2.

    abstract::Low temperature is a seasonal abiotic stress that restricts native plant ranges and crop distributions. Two types of low-temperature stress can be distinguished: chilling and freezing. Much work has been done on the mechanisms by which chilling is sensed, but relatively little is known about how plants sense freezing....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00286

    authors: Barnes AC,Benning C,Roston RL

    更新日期:2016-07-01 00:00:00

  • Purification and Characterization of the Enzymes of Fructan Biosynthesis in Tubers of Helianthus tuberosus Colombia (II. Purification of Sucrose:Sucrose 1-Fructosyltransferase and Reconstitution of Fructan Synthesis in Vitro with Purified Sucrose:Sucrose

    abstract::Sucrose:sucrose 1-fructosyltransferase (1-SST), an enzyme involved in fructan biosynthesis, was purified to homogeneity from tubers of Helianthus tuberosus that were harvested in the accumulation phase. Gel filtration under native conditions predicted a molecular mass of about 67 kD. Electrophoresis or gel filtration ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.4.1167

    authors: Koops AJ,Jonker HH

    更新日期:1996-04-01 00:00:00

  • Identification of a specific isoform of tomato lipoxygenase (TomloxC) involved in the generation of fatty acid-derived flavor compounds.

    abstract::There are at least five lipoxygenases (TomloxA, TomloxB, TomloxC, TomloxD, and TomloxE) present in tomato (Lycopersicon esculentum Mill.) fruit, but their role in generation of fruit flavor volatiles has been unclear. To assess the physiological role of TomloxC in the generation of volatile C6 aldehyde and alcohol fla...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.041608

    authors: Chen G,Hackett R,Walker D,Taylor A,Lin Z,Grierson D

    更新日期:2004-09-01 00:00:00

  • Arabidopsis to rice. Applying knowledge from a weed to enhance our understanding of a crop species.

    abstract::Although Arabidopsis is well established as the premiere model species in plant biology, rice (Oryza sativa) is moving up fast as the second-best model organism. In addition to the availability of large sets of genetic, molecular, and genomic resources, two features make rice attractive as a model species: it represen...

    journal_title:Plant physiology

    pub_type: 杂志文章,评审

    doi:10.1104/pp.104.040170

    authors: Rensink WA,Buell CR

    更新日期:2004-06-01 00:00:00

  • Uptake of bicarbonate ion in darkness by isolated chloroplast envelope membranes and intact chloroplasts of spinach.

    abstract::Bicarbonate uptake by isolated chloroplast envelope membranes and intact chloroplasts of spinach (Spinacia oleracea L. var. Viroflay) in darkness exhibited a similar dependency upon temperature, pH, time, and concentrations of isolated or attached envelope membranes. This similarity in uptake properties demonstrates t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.4.520

    authors: Poincelot RP

    更新日期:1974-10-01 00:00:00

  • Role of polygalacturonase in bean leaf abscission.

    abstract::The role of polygalacturonase in leaf abscission was studied in explants of Phaseolus vulgaris L. cv. Red Kidney. Bean polygalacturonase was partially characterized and comparisons were made between the bean enzyme and previously reported higher plant polygalacturonases. Polygalacturonase isolated from bean leaf absci...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.6.1133

    authors: Berger RK,Reid PD

    更新日期:1979-06-01 00:00:00

  • Light sensitivity of na, rb, and k absorption by different tissues of bean leaves.

    abstract::Autoradiographs of (22)Na-loaded bean (Phaseolus vulgaris L. cv. ;Brittle Wax') leaf slices showed that most of the tracer was concentrated in vascular tissue. Rubidium-86 was uniformly distributed in slices that had been incubated in darkness; after incubation in the light many small dark spots appeared on the autora...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.6.978

    authors: Jacoby B

    更新日期:1975-06-01 00:00:00

  • Stomatal Responses to Light and Drought Stress in Variegated Leaves of Hedera helix.

    abstract::Direct and indirect mechanisms underlying the light response of stomata were studied in variegated leaves of the juvenile phase of Hedera helix L. Dose response curves of leaf conductance were measured with blue and red light in leaves kept in normal or in an inverted position. In the green portions of the leaves, the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.81.3.768

    authors: Aphalo PJ,Sánchez RA

    更新日期:1986-07-01 00:00:00

  • Transcriptional profiling of the Arabidopsis embryo.

    abstract::We have used laser-capture microdissection to isolate RNA from discrete tissues of globular, heart, and torpedo stage embryos of Arabidopsis (Arabidopsis thaliana). This was amplified and analyzed by DNA microarray using the Affymetrix ATH1 GeneChip, representing approximately 22,800 Arabidopsis genes. Cluster analysi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.087668

    authors: Spencer MW,Casson SA,Lindsey K

    更新日期:2007-02-01 00:00:00

  • Cytochemical localization of glycolate dehydrogenase in mitochondria of chlamydomonas.

    abstract::Mildly disrupted cells of Chlamydomonas reinhardi Dangeard were incubated in a reaction medium containing glycolate, ferricyanide, and cupric ions, and then processed for electron microscopy. As a result of the cytochemical treatment, an electron opaque product was deposited specifically in the outer compartment of mi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.3.315

    authors: Beezley BB,Gruber PJ,Frederick SE

    更新日期:1976-09-01 00:00:00

  • Unique Motifs and Length of Hairpin in Oleosin Target the Cytosolic Side of Endoplasmic Reticulum and Budding Lipid Droplet.

    abstract::Plant cytosolic lipid droplets (LDs) are covered with a layer of phospholipids and oleosin and were extensively studied before those in mammals and yeast. Oleosin has short amphipathic N- and C-terminal peptides flanking a conserved 72-residue hydrophobic hairpin, which penetrates and stabilizes the LD Oleosin is synt...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00366

    authors: Huang CY,Huang AHC

    更新日期:2017-08-01 00:00:00

  • Isolation of a cDNA encoding a granule-bound 152-kilodalton starch-branching enzyme in wheat.

    abstract::Screening of a wheat (Triticum aestivum) cDNA library for starch-branching enzyme I (SBEI) genes combined with 5'-rapid amplification of cDNA ends resulted in isolation of a 4,563-bp composite cDNA, Sbe1c. Based on sequence alignment to characterized SBEI cDNA clones isolated from plants, the SBEIc predicted from the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.124.1.253

    authors: Båga M,Nair RB,Repellin A,Scoles GJ,Chibbar RN

    更新日期:2000-09-01 00:00:00

  • Marker-free transgenic plants through genetically programmed auto-excision.

    abstract::We present here a vector system to obtain homozygous marker-free transgenic plants without the need of extra handling and within the same time frame as compared to transformation methods in which the marker is not removed. By introducing a germline-specific auto-excision vector containing a cre recombinase gene under ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.106526

    authors: Verweire D,Verleyen K,De Buck S,Claeys M,Angenon G

    更新日期:2007-12-01 00:00:00

  • Identification of Intracellular Carbonic Anhydrase in Chlamydomonas reinhardtii which Is Distinct from the Periplasmic Form of the Enzyme.

    abstract::A physiologically significant level of intracellular carbonic anhydrase has been identified in Chlamydomonas reinhardtii after lysis of the cell wall-less mutant, cw15, and two intracellular polypeptides have been identified which bind to anti-carbonic anhydrase antisera. The susceptibility of the intracellular activi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.3.904

    authors: Husic HD,Kitayama M,Togasaki RK,Moroney JV,Morris KL,Tolbert NE

    更新日期:1989-03-01 00:00:00

  • The DEK1 Calpain Linker Functions in Three-Dimensional Body Patterning in Physcomitrella patens.

    abstract::The DEFECTIVE KERNEL1 (DEK1) calpain is a conserved 240-kD key regulator of three-dimensional body patterning in land plants acting via mitotic cell plane positioning. The activity of the cytosolic C-terminal calpain protease is regulated by the membrane-anchored DEK1 MEM, which is connected to the calpain via the 600...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00925

    authors: Johansen W,Ako AE,Demko V,Perroud PF,Rensing SA,Mekhlif AK,Olsen OA

    更新日期:2016-10-01 00:00:00

  • Interaction of the Arabidopsis E2F and DP proteins confers their concomitant nuclear translocation and transactivation.

    abstract::E2F transcription factors are required for the progression and arrest of the cell cycle in animals. Like animals, plants have evolved to conserve the E2F family. The Arabidopsis genome encodes E2F and DP proteins that share a high similarity with the animal E2F and DP families. Here, we show that Arabidopsis E2F and D...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.010642

    authors: Kosugi S,Ohashi Y

    更新日期:2002-03-01 00:00:00

  • Involvement of phytosulfokine in the attenuation of stress response during the transdifferentiation of zinnia mesophyll cells into tracheary elements.

    abstract::Phytosulfokine (PSK) is a sulfated peptide hormone required for the proliferation and differentiation of plant cells. Here, we characterize the physiological roles of PSK in transdifferentiation of isolated mesophyll cells of zinnia (Zinnia elegans 'Canary Bird') into tracheary elements (TEs). Transcripts for a zinnia...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.135954

    authors: Motose H,Iwamoto K,Endo S,Demura T,Sakagami Y,Matsubayashi Y,Moore KL,Fukuda H

    更新日期:2009-05-01 00:00:00

  • HEAT SHOCK FACTOR A8a Modulates Flavonoid Synthesis and Drought Tolerance.

    abstract::Drought is an important environmental factor affecting the growth and production of agricultural crops and fruits worldwide, including apple (Malus domestica). Heat shock factors (HSFs) have well-documented functions in stress responses, but their roles in flavonoid synthesis and the flavonoid-mediated drought respons...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.01106

    authors: Wang N,Liu W,Yu L,Guo Z,Chen Z,Jiang S,Xu H,Fang H,Wang Y,Zhang Z,Chen X

    更新日期:2020-11-01 00:00:00

  • Errors in the Measurement of Root Pressure and Exudation Volume Flow Rate Caused by Damage during the Transfer of Unsupported Roots between Solutions.

    abstract::Plants of Zea mays were grown with their roots confined to growing tubes, consisting of cylindrical or spherical glass tubes fitted at the bottom with a stopcock. Nutrient solution was circulated past the roots, and when a plant was 21 to 25 days old, the stopcock was closed, the root excised from the plant and connec...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.1.164

    authors: Miller DM

    更新日期:1987-09-01 00:00:00

  • Uptake and phytotoxicity of the herbicide metsulfuron methyl in corn root tissue in the presence of the safener 1,8-naphthalic anhydride.

    abstract::Growth of Zea mays L. cv Potro roots was inhibited by the herbicide metsulfuron methyl (MSM) at the lowest concentration tested: 5 nanomoles per liter. Pretreatment of corn seeds with commercial 1,8-naphthalic anhydride (NA) at 1% (w/w) partially reversed MSM-induced root growth inhibition. MSM at a concentration of 5...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.2.730

    authors: Milhomme H,Bastide J

    更新日期:1990-06-01 00:00:00

  • Immunolocalization of a Unique Form of Maize Kernel Glutamine Synthetase Using a Monoclonal Antibody.

    abstract::The pedicel (basal maternal tissue) of maize (Zea mays L.) kernels contains a physically and kinetically unique form of glutamine synthetase (GSp1) that is involved in the conversion of transport forms of nitrogen into glutamine for uptake by the developing endosperm (M.J. Muhitch [1989] Plant Physiol 91: 868-875). A ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.3.757

    authors: Muhitch MJ,Felker FC,Taliercio EW,Chourey PS

    更新日期:1995-03-01 00:00:00

  • Feruloyl-CoA 6'-Hydroxylase1-dependent coumarins mediate iron acquisition from alkaline substrates in Arabidopsis.

    abstract::Although iron (Fe) is one of the most abundant elements in the earth's crust, its low solubility in soils restricts Fe uptake by plants. Most plant species acquire Fe by acidifying the rhizosphere and reducing ferric to ferrous Fe prior to membrane transport. However, it is unclear how these plants access Fe in the rh...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.228544

    authors: Schmid NB,Giehl RF,Döll S,Mock HP,Strehmel N,Scheel D,Kong X,Hider RC,von Wirén N

    更新日期:2014-01-01 00:00:00