Identification of Intracellular Carbonic Anhydrase in Chlamydomonas reinhardtii which Is Distinct from the Periplasmic Form of the Enzyme.

abstract：:Carbon-isotope discrimination (Delta) is used to distinguish between different photosynthetic pathways. It has also been shown that variation in Delta occurs among varieties of C(3) species, but not as yet, in C(4) species. We now report that Delta also varies among genotypes of sorghum (Sorghum bicolor Moench), a C(4...

journal_title：Plant physiology

authors： Hubick KT,Hammer GL,Farquhar GD,Wade LJ,von Caemmerer S,Henderson SA

更新日期：1990-02-01 00:00:00

abstract：:The existence of sphingolipid- and sterol-enriched microdomains, known as lipid rafts, in the plasma membrane (PM) of eukaryotic cells is well documented. To obtain more insight into the lipid molecular species required for the formation of microdomains in plants, we have isolated detergent (Triton X-100)-resistant me...

journal_title：Plant physiology

authors： Laloi M,Perret AM,Chatre L,Melser S,Cantrel C,Vaultier MN,Zachowski A,Bathany K,Schmitter JM,Vallet M,Lessire R,Hartmann MA,Moreau P

更新日期：2007-01-01 00:00:00

abstract：:Selenium-Binding Protein1 (SBP1) gene expression was studied in Arabidopsis (Arabidopsis thaliana) seedlings challenged with several stresses, including cadmium (Cd), selenium {selenate [Se(VI)] and selenite [Se(IV)]}, copper (Cu), zinc (Zn), and hydrogen peroxide (H(2)O(2)) using transgenic lines expressing the lucif...

journal_title：Plant physiology

authors： Hugouvieux V,Dutilleul C,Jourdain A,Reynaud F,Lopez V,Bourguignon J

更新日期：2009-10-01 00:00:00

abstract：:Plant architecture is determined by genetic and developmental programs as well as by environmental factors. Sessile plants have evolved a subtle adaptive mechanism that allows them to alter their growth and development during periods of stress. Phytohormones play a central role in this process; however, the molecules ...

journal_title：Plant physiology

authors： Zhang SW,Li CH,Cao J,Zhang YC,Zhang SQ,Xia YF,Sun DY,Sun Y

更新日期：2009-12-01 00:00:00

abstract：:In contrast to angiosperms, pines and other gymnosperms form well-developed suspensors in somatic embryogenic cultures. This creates a useful system to study suspensor biology. In a study of gene expression during the early stages of conifer embryogenesis, we identified a transcript, PtNIP1;1, that is abundant in imma...

journal_title：Plant physiology

authors： Ciavatta VT,Morillon R,Pullman GS,Chrispeels MJ,Cairney J

更新日期：2001-12-01 00:00:00

abstract：:Sucrose:sucrose 1-fructosyltransferase (1-SST), an enzyme involved in fructan biosynthesis, was purified to homogeneity from tubers of Helianthus tuberosus that were harvested in the accumulation phase. Gel filtration under native conditions predicted a molecular mass of about 67 kD. Electrophoresis or gel filtration ...

journal_title：Plant physiology

authors： Koops AJ,Jonker HH

更新日期：1996-04-01 00:00:00

abstract：:A method for the preparation of developmentally staged microspores and young pollen from maize (Zea mays) has been devised. The preparations are of sufficient purity and quantity for biochemical analysis, including the analysis of steady-state protein and RNA populations associated with each stage. A major transition ...

journal_title：Plant physiology

authors： Bedinger PA,Edgerton MD

更新日期：1990-02-01 00:00:00

abstract：:Expressed sequence tags (ESTs) currently encompass more entries in the public databases than any other form of sequence data. Thus, EST data sets provide a vast resource for gene identification and expression profiling. We have mapped the complete set of 176,915 publicly available Arabidopsis EST sequences onto the Ar...

journal_title：Plant physiology

authors： Zhu W,Schlueter SD,Brendel V

更新日期：2003-06-01 00:00:00

abstract：:Computing techniques are used to simulate the course of uptake of K(+), Na(+), and Cl(-) by low-salt roots. Measurements of the fluxes of these ions in high-salt roots are used to calculate membrane permeabilities, which are then used to calculate cell uptake. In this way it is possible to test the predictive value of...

journal_title：Plant physiology

authors： Pitman MG

更新日期：1969-10-01 00:00:00

abstract：:Rubisco catalyzes the fixation of CO2 into organic compounds that are used for plant growth and the production of agricultural products, and specific sugar-phosphate derivatives bind tightly to the active sites of Rubisco, locking the enzyme in a catalytically inactive conformation. 2-carboxy-d-arabinitol-1-phosphate ...

journal_title：Plant physiology

authors： Lobo AKM,Orr DJ,Gutierrez MO,Andralojc PJ,Sparks C,Parry MAJ,Carmo-Silva E

更新日期：2019-10-01 00:00:00

abstract：:Disc electrophoresis was used to separate water soluble proteins from hardy, non-hardy, and frost killed cabbage (Brassica oleracea var. capitata) leaves. Amidoschwarz staining failed to reveal any new bands as a result of hardening although the relative amounts of proteins in individual bands changed. Sulfhydryl grou...

journal_title：Plant physiology

authors： Morton WM

更新日期：1969-02-01 00:00:00

abstract：:The FAD7 gene is expressed preferentially in the chlorophyllous tissues of unwounded plants. Wounding activates the expression of the FAD7 gene not only in chlorophyllous tissues, but also in nonchlorophyllous tissues of stems and roots. Our previous study suggested that wound-responsive transcriptional activation by ...

journal_title：Plant physiology

authors： Nishiuchi T,Kodama H,Yanagisawa S,Iba K

更新日期：1999-12-01 00:00:00

abstract：:This paper describes the assembly (from commercially available components) of an antilog converter, which transforms the output signals of ion-specific electrodes to ionic concentrations suitable for a linear recorder. It responds linearly to cation concentrations from 10 mum to at least 10 mm and can be used for elec...

journal_title：Plant physiology

authors： Heath RL

更新日期：1975-08-01 00:00:00

abstract：:Magnaporthe oryzae is a fungal pathogen that causes rice (Oryza sativa) blast. SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) are key components in vesicle trafficking in eukaryotic cells and are known to contribute to fungal pathogen resistance. Syntaxin of Plants121 (SYP121), a Qa-SN...

journal_title：Plant physiology

authors： Cao WL,Yu Y,Li MY,Luo J,Wang RS,Tang HJ,Huang J,Wang JF,Zhang HS,Bao YM

更新日期：2019-04-01 00:00:00

abstract：:The ontogeny of seed structure and the accumulation of seed storage substances is the result of a determinant genetic program. Using RNA interference, the synthesis of soybean (Glycine max) glycinin and conglycinin storage proteins has been suppressed. The storage protein knockdown (SP-) seeds are overtly identical to...

journal_title：Plant physiology

authors： Schmidt MA,Barbazuk WB,Sandford M,May G,Song Z,Zhou W,Nikolau BJ,Herman EM

更新日期：2011-05-01 00:00:00

abstract：:Adenosine kinase was partially purified from wheat germ. This enzyme preparation, which was devoid of adenine phosphoribosyltransferase and nearly free of adenosine deaminase but contained adenylate kinase, rapidly phosphorylated adenosine and a cytokinin, N(6)-(delta(2)-isopentenyl)adenosine. Electrophoretic analysis...

journal_title：Plant physiology

authors： Chen CM,Eckert RL

更新日期：1977-03-01 00:00:00

abstract：:DNA and histone methylation coregulate heterochromatin formation and gene silencing in animals and plants. To identify factors involved in maintaining gene silencing, we conducted a forward genetic screen for mutants that release the silenced transgene Pro35S::NEOMYCIN PHOSPHOTRANSFERASE II in the transgenic Arabidops...

journal_title：Plant physiology

authors： Meng J,Wang L,Wang J,Zhao X,Cheng J,Yu W,Jin D,Li Q,Gong Z

更新日期：2018-06-01 00:00:00

abstract：:The response of the actin cytoskeleton to nodulation (Nod) factors secreted by Rhizobium etli has been studied in living root hairs of bean (Phaseolus vulgaris) that were microinjected with fluorescein isothiocyanate-phalloidin. In untreated control cells or cells treated with the inactive chitin oligomer, the actin c...

journal_title：Plant physiology

authors： Crdenas L,Vidali L,Domnguez J,Prez H,Snchez F,Hepler PK,Quinto C

更新日期：1998-03-01 00:00:00

abstract：:We have introduced an ice nucleation gene (inaZ) from Pseudomonas syringae pv. syringae into Nicotiana tabacum, a freezing-sensitive species, and Solanum commersonii, a freezing-tolerant species. Transformants of both species showed increased ice nucleation activity over untransformed controls. The concentration of ic...

journal_title：Plant physiology

authors： Baertlein DA,Lindow SE,Panopoulos NJ,Lee SP,Mindrinos MN,Chen TH

更新日期：1992-12-01 00:00:00

abstract：:Phosphorus (P) is an essential macronutrient, and P deficiency limits plant productivity. Recent work showed that P deficiency affects electron transport to photosystem I (PSI), but the underlying mechanisms are unknown. Here, we present a comprehensive biological model describing how P deficiency disrupts the photosy...

journal_title：Plant physiology

authors： Carstensen A,Herdean A,Schmidt SB,Sharma A,Spetea C,Pribil M,Husted S

更新日期：2018-05-01 00:00:00

abstract：:The photosynthetic potential of leaves and chloroembryos of Cyamopsis tetragonoloba (L.) Taub as measured by (14)C-bicarbonate fixation, Hill activity, and in vivo fluorescence transients is compared. On a chlorophyll basis, dark fixation of NaH(14)CO(3) in chloroembryos was 1.5 times higher than that of the leaf, whe...

journal_title：Plant physiology

authors： Kaladharan P,Vivekanandan M

更新日期：1990-02-01 00:00:00

abstract：:Chloroplast NADPH-thioredoxin reductase (NTRC) belongs to the thioredoxin systems that control crucial metabolic and regulatory pathways in plants. Here, by characterization of T-DNA insertion lines of NTRC gene, we uncover a novel connection between chloroplast thiol redox regulation and the control of photoperiodic ...

journal_title：Plant physiology

authors： Lepistö A,Kangasjärvi S,Luomala EM,Brader G,Sipari N,Keränen M,Keinänen M,Rintamäki E

更新日期：2009-03-01 00:00:00

abstract：:A key floral activator, FT, integrates stimuli from long-day, vernalization, and autonomous pathways and triggers flowering by directly regulating floral meristem identity genes in Arabidopsis (Arabidopsis thaliana). Since a small amount of FT transcript is sufficient for flowering, the FT level is strictly regulated ...

journal_title：Plant physiology

authors： Seo E,Yu J,Ryu KH,Lee MM,Lee I

更新日期：2011-08-01 00:00:00

abstract：:Aspartate aminotransferase (AAT), a key enzyme in the biosynthesis of aspartate and asparagine, occurs as two forms in alfalfa (Medicago sativa L.), AAT-1 and AAT-2. Both forms were purified to near homogeneity, and high titer polyclonal antibodies produced to the native proteins. Alfalfa AAT-1 was purified from root ...

journal_title：Plant physiology

authors： Farnham MW,Miller SS,Griffith SM,Vance CP

更新日期：1990-06-01 00:00:00

abstract：:The unicellular green alga Chlamydomonas reinhardtii is a widely used model organism for studies of oxygenic photosynthesis in eukaryotes. Here we describe the development of a resource for functional genomics of photosynthesis using insertional mutagenesis of the Chlamydomonas nuclear genome. Chlamydomonas cells were...

journal_title：Plant physiology

authors： Dent RM,Haglund CM,Chin BL,Kobayashi MC,Niyogi KK

更新日期：2005-02-01 00:00:00

abstract：:All aerobic biological systems, including N(2)-fixing root nodules, are subject to O(2) toxicity that results from the formation of reactive intermediates such as H(2)O(2) and free radicals of O(2). H(2)O(2) may be removed from root nodules in a series of enzymic reactions involving ascorbate peroxidase, dehydroascorb...

journal_title：Plant physiology

authors： Dalton DA,Hanus FJ,Russell SA,Evans HJ

更新日期：1987-04-01 00:00:00

abstract：:A procedure is described that can be used to minimize toxic effects of polyethylene glycol (PEG) to plants. The procedure is based on recycling nutrient solutions containing PEG-6000 through two plant cultures. Tomato plants grown in -0.3 megapascals PEG solutions used after two growth cycles exhibited minimal toxic e...

journal_title：Plant physiology

authors： Plaut Z,Federman E

更新日期：1985-10-01 00:00:00

abstract：:The correct separation of homologous chromosomes during meiosis I, and sister chromatids during meiosis II, relies on the tight control of the cohesion complex. The phosphorylation and subsequent cleavage of the meiotic recombination protein REC8 (REC8-like family protein [SYN1] in Arabidopsis [Arabidopsis thaliana]),...

journal_title：Plant physiology

authors： Yuan G,Ahootapeh BH,Komaki S,Schnittger A,Lillo C,De Storme N,Geelen D

更新日期：2018-09-01 00:00:00

abstract：:Sucrose gradient profiles of polyribosomes from the coleoptilar node region of seedlings of Zea mays L. were obtained without pelleting and redispersion of the particles. Water stress caused a shift of ribosomes from the polymeric to the monomeric form, starting about 30 minutes after stress initiation and when the wa...

journal_title：Plant physiology

authors： Hsiao TC

更新日期：1970-08-01 00:00:00

abstract：:Tomato (Lycopersicon esculentum Mill. cv Ailsa Craig) fruit, at five stages of development, have been analyzed for their carotenoid and chlorophyll (Chl) contents, in vitro activities of phytoene synthase, phytoene desaturase, and lycopene cyclase, as well as expression of the phytoene synthase (Psy) and phytoene desa...

journal_title：Plant physiology

authors： Fraser PD,Truesdale MR,Bird CR,Schuch W,Bramley PM

更新日期：1994-05-01 00:00:00