Abstract：
:Adenosine kinase was partially purified from wheat germ. This enzyme preparation, which was devoid of adenine phosphoribosyltransferase and nearly free of adenosine deaminase but contained adenylate kinase, rapidly phosphorylated adenosine and a cytokinin, N(6)-(delta(2)-isopentenyl)adenosine. Electrophoretic analysis indicated that only N(6)-(delta(2)-isopentenyl)adenosine-monophosphate was formed from the cytokinin while about 55% AMP, 45% ADP, and a trace of ATP were formed from adenosine. The biosynthesized nucleoside monophosphates were quantitatively hydrolyzed to the corresponding nucleosides by 5'-nucleotidase and the isopentenyl side chain of the phosphorylated cytokinin was not cleaved. The enzyme did not catalyze phosphorylation of inosine.The phosphorylation of the cytokinin and adenosine required ATP and Mg(2+). The pH optimum was from 6.8 to 7.2 for both the cytokinin and adenosine. At pH 7 and 37 C the Km and V(max) for the cytokinin were 31 mum and 8.3 nmoles per mg protein per minute, and the values for adenosine were 8.7 mum and 46 nmoles per mg protein per minute. Crude enzyme preparations from tobacco callus tissue and wheat germ phosphorylated N(6)-(delta(2)-isopentenyl)adenosine. These preparations also phosphorylated N(6)-(delta(2)-isopentenyl)adenine when 5-phosphorylribose-1-pyrophosphate was present.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Chen CM,Eckert RLdoi
10.1104/pp.59.3.443subject
Has Abstractpub_date
1977-03-01 00:00:00pages
443-7issue
3eissn
0032-0889issn
1532-2548journal_volume
59pub_type
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