Starch Turnover and Metabolism during Flower and Early Embryo Development.

Abstract:

:The accumulation of starch within photosynthetic tissues and within dedicated storage organs has been characterized extensively in many species, and a function in buffering carbon availability or in fueling later growth phases, respectively, has been proposed. However, developmentally regulated starch turnover within heterotrophic tissues other than dedicated storage organs is poorly characterized, and its function is not well understood. Here, we report on the characterization of starch turnover during flower, early embryo, and silique development in Arabidopsis (Arabidopsis thaliana) using a combined clearing-staining technique on whole-mount tissue. Besides the two previously documented waves of transient starch accumulation in the stamen envelope, occurring during meiosis and pollen mitosis I, we identified a novel, third wave of starch amylogenesis/amylolysis during the last stages of stamen development. To gain insights into the underlying molecular mechanisms, we analyzed publicly available microarray data, which revealed a developmentally coordinated expression of carbohydrate transport and metabolism genes during these waves of transient starch accumulation. Based on this analysis, we characterized starch dynamics in mutants affecting hexose phosphate metabolism and translocation, and identified the Glc-6-phosphate/phosphate antiporter GPT1 as the putative translocator of Glc-6-phosphate for starch biosynthesis in reproductive tissues. Based on these results, we propose a model of starch synthesis within the pollen grain and discuss the nutrient transport route feeding the embryo within the developing seed.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Hedhly A,Vogler H,Schmid MW,Pazmino D,Gagliardini V,Santelia D,Grossniklaus U

doi

10.1104/pp.16.00916

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

2388-2402

issue

4

eissn

0032-0889

issn

1532-2548

pii

pp.16.00916

journal_volume

172

pub_type

杂志文章
  • Effects of temperature, water potential, and light on germination responses of redroot pigweed seeds to ethylene.

    abstract::The responses of redroot pigweed (Amaranthus retroflexus L.) seeds to nine ethylene concentrations between 0.5 and 50 microliters per liter were assessed at different temperatures and water potentials and in either continuous white light or darkness. Under all experimental treatments, the probit-transformed percentage...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.6.1149

    authors: Schonbeck MW,Egley GH

    更新日期:1980-06-01 00:00:00

  • A bioinformatics approach to the identification, classification, and analysis of hydroxyproline-rich glycoproteins.

    abstract::Hydroxyproline-rich glycoproteins (HRGPs) are a superfamily of plant cell wall proteins that function in diverse aspects of plant growth and development. This superfamily consists of three members: hyperglycosylated arabinogalactan proteins (AGPs), moderately glycosylated extensins (EXTs), and lightly glycosylated pro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.156554

    authors: Showalter AM,Keppler B,Lichtenberg J,Gu D,Welch LR

    更新日期:2010-06-01 00:00:00

  • Overexpression of a homeobox gene, LeT6, reveals indeterminate features in the tomato compound leaf

    abstract::The cultivated tomato (Lycopersicon esculentum) has a unipinnate compound leaf. In the developing leaf primordium, major leaflet initiation is basipetal, and lobe formation and early vascular differentiation are acropetal. We show that engineered alterations in the expression of a tomato homeobox gene, LeT6, can cause...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.3.771

    authors: Janssen BJ,Lund L,Sinha N

    更新日期:1998-07-01 00:00:00

  • Isolation of Intact and Functional Chloroplasts from Mesophyll and Bundle Sheath Protoplasts of the C(4) Plant Panicum miliaceum.

    abstract::A procedure is described for isolating and purifying mesophyll protoplasts and bundle sheath protoplasts of the C(4) plant Panicum miliaceum. Following enzymic digestion of leaf tissue, mesophyll protoplasts and bundle sheath protoplasts are released and purified by density centrifugation. The lower density of mesophy...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.5.821

    authors: Edwards GE

    更新日期:1979-05-01 00:00:00

  • Mesoporous silica nanoparticle-mediated intracellular cre protein delivery for maize genome editing via loxP site excision.

    abstract::The delivery of proteins instead of DNA into plant cells allows for a transient presence of the protein or enzyme that can be useful for biochemical analysis or genome modifications. This may be of particular interest for genome editing, because it can avoid DNA (transgene) integration into the genome and generate pre...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.233650

    authors: Martin-Ortigosa S,Peterson DJ,Valenstein JS,Lin VS,Trewyn BG,Lyznik LA,Wang K

    更新日期:2014-02-01 00:00:00

  • LeCTR1, a tomato CTR1-like gene, demonstrates ethylene signaling ability in Arabidopsis and novel expression patterns in tomato.

    abstract::LeCTR1 was initially isolated by both differential display reverse transcriptase-polymerase chain reaction screening for tomato (Lycopersicon esculentum) fruit ethylene-inducible genes and through homology with the Arabidopsis CTR1 cDNA. LeCTR1 shares strong nucleotide sequence homology with Arabidopsis CTR1, a gene a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.009415

    authors: Leclercq J,Adams-Phillips LC,Zegzouti H,Jones B,Latché A,Giovannoni JJ,Pech JC,Bouzayen M

    更新日期:2002-11-01 00:00:00

  • Effect of water stress on cell division and cell-division-cycle 2-like cell-cycle kinase activity in wheat leaves

    abstract::In wheat (Triticum aestivum) seedlings subjected to a mild water stress (water potential of -0.3 MPa), the leaf-elongation rate was reduced by one-half and the mitotic activity of mesophyll cells was reduced to 42% of well-watered controls within 1 d. There was also a reduction in the length of the zone of mesophyll c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.2.667

    authors: Schuppler U,He PH,John PC,Munns R

    更新日期:1998-06-01 00:00:00

  • In Vivo and in Vitro Studies of Glucose-6-Phosphate Dehydrogenase from Barley Root Plastids in Relation to Reductant Supply for NO2- Assimilation.

    abstract::Pyridine nucleotide pools were measured in intact plastids from roots of barley (Hordeum vulgare L.) during the onset of NO2- assimilation and compared with the in vitro effect of the NADPH/NADP ratio on the activity of plastidic glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) from N-sufficient or N-starved roo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.4.1413

    authors: Wright DP,Huppe HC,Turpin DH

    更新日期:1997-08-01 00:00:00

  • Variability in Proline-Accumulating Ability of Barley (Hordeum vulgare L.) Cultivars Induced by Vapor Pressure Deficit.

    abstract::This work was undertaken in an effort to reconcile the conflicting proline-accumulating responses of the barley (Hordeum vulgare L.) cultivars, Excelsior and Proctor, reported by Singh et al. (1972) and Hanson et al. (1976). It deals with the effects of different vapor pressure deficits (VPD) during growth and subsequ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.98.2.716

    authors: Naidu BP,Aspinall D,Paleg LG

    更新日期:1992-02-01 00:00:00

  • Hydrotropism interacts with gravitropism by degrading amyloplasts in seedling roots of Arabidopsis and radish.

    abstract::In response to a moisture gradient, roots exhibit hydrotropism to control the orientation of their growth. To exhibit hydrotropism, however, they must overcome the gravitropism that is dominant on Earth. We found that moisture gradient or water stress caused immediate degradation of the starch anchors, amyloplasts, in...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.018853

    authors: Takahashi N,Yamazaki Y,Kobayashi A,Higashitani A,Takahashi H

    更新日期:2003-06-01 00:00:00

  • Selection for Improved Energy Use Efficiency and Drought Tolerance in Canola Results in Distinct Transcriptome and Epigenome Changes.

    abstract::To increase both the yield potential and stability of crops, integrated breeding strategies are used that have mostly a direct genetic basis, but the utility of epigenetics to improve complex traits is unclear. A better understanding of the status of the epigenome and its contribution to agronomic performance would he...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00155

    authors: Verkest A,Byzova M,Martens C,Willems P,Verwulgen T,Slabbinck B,Rombaut D,Van de Velde J,Vandepoele K,Standaert E,Peeters M,Van Lijsebettens M,Van Breusegem F,De Block M

    更新日期:2015-08-01 00:00:00

  • Resistance to low temperature photoinhibition is not associated with isolated thylakoid membranes of winter rye.

    abstract::In vivo measurements of chlorophyll a fluorescence indicate that cold-hardened winter rye (Secale cereale L. cv Musketeer) develops a resistance to low temperature-induced photoinhibition compared with nonhardened rye. After 7.2 hours at 5 degrees C and 1550 micromoles per square meter per second, the ratio of variabl...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.97.2.804

    authors: Lapointe L,Huner NP,Carpentier R,Ottander C

    更新日期:1991-10-01 00:00:00

  • Leaf Energy Balance Requires Mitochondrial Respiration and Export of Chloroplast NADPH in the Light.

    abstract::Key aspects of leaf mitochondrial metabolism in the light remain unresolved. For example, there is debate about the relative importance of exporting reducing equivalents from mitochondria for the peroxisomal steps of photorespiration versus oxidation of NADH to generate ATP by oxidative phosphorylation. Here, we addre...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00624

    authors: Shameer S,Ratcliffe RG,Sweetlove LJ

    更新日期:2019-08-01 00:00:00

  • Amino Acid uptake by pea leaf fragments: specificity, energy sources, and mechanism.

    abstract::Amino acid uptake into leaf fragments of Pisum sativum depended on metabolism. Glycine uptake was optimal at 30 C and could be supported by respiration and by photosynthesis. Based on studies with an electron flow cofactor, inhibitors, and uncouplers, the energy source for glycine uptake was apparently ATP.The energy-...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.52.6.633

    authors: Cheung YN,Nobel PS

    更新日期:1973-12-01 00:00:00

  • Leaf Conductance in Relation to Assimilation in Eucalyptus pauciflora Sieb. ex Spreng: Influence of Irradiance and Partial Pressure of Carbon Dioxide.

    abstract::Rates of assimilation and transpiration in Eucalyptus pauciflora Sieb. ex Spreng were measured at various ambient partial pressures of CO(2) and various irradiances and were used to estimate leaf conductance and intercellular partial pressure of CO(2). The responses of leaf conductance and rate of assimilation to chan...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.4.670

    authors: Wong SC,Cowan IR,Farquhar GD

    更新日期:1978-10-01 00:00:00

  • Resolution and Properties of Two High Affinity Cyclic Adenosine 3':5'-monophosphate-Binding Proteins from Wheat Germ.

    abstract::A high affinity cAMP-binding protein (cABP II) was purified to homogeneity from wheat germ. The apparent molecular weight of cABP II, as determined from gel exclusion chromatography, is 5.2 x 10(5) (at low ionic strength) and 2.8 x 10(5) (at high ionic strength). One polypeptide subunit (molecular weight, 80,000) was ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.3.577

    authors: Polya GM,Bowman JA

    更新日期:1981-09-01 00:00:00

  • Photoperiod Affects the Phenotype of Mitochondrial Complex I Mutants.

    abstract::Plant mutants for genes encoding subunits of mitochondrial complex I (CI; NADH:ubiquinone oxidoreductase), the first enzyme of the respiratory chain, display various phenotypes depending on growth conditions. Here, we examined the impact of photoperiod, a major environmental factor controlling plant development, on tw...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01484

    authors: Pétriacq P,de Bont L,Genestout L,Hao J,Laureau C,Florez-Sarasa I,Rzigui T,Queval G,Gilard F,Mauve C,Guérard F,Lamothe-Sibold M,Marion J,Fresneau C,Brown S,Danon A,Krieger-Liszkay A,Berthomé R,Ribas-Carbo M,Tcherkez

    更新日期:2017-01-01 00:00:00

  • A Plant Virus-Based Vector System for Gene Function Studies in Pepper.

    abstract::While pepper (Capsicum annuum) is a highly recalcitrant species for genetic transformation studies, plant virus-based vectors can provide alternative and powerful tools for transient regulation and functional analysis of genes of interest in pepper. In this study, we established an effective virus-based vector system ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00836

    authors: Choi B,Kwon SJ,Kim MH,Choe S,Kwak HR,Kim MK,Jung C,Seo JK

    更新日期:2019-11-01 00:00:00

  • Partitioning of Intermediary Carbon Metabolism in Vesicular-Arbuscular Mycorrhizal Leek.

    abstract::Vesicular-arbuscular mycorrhizal fungi are symbionts for a large variety of crop plants; however, the form in which they take up carbon from the host is not established. To trace the course of carbon metabolism, we have used nuclear magnetic resonance spectroscopy with [13C]glucose labeling in vivo and in extracts to ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.1.7

    authors: Shachar-Hill Y,Pfeffer PE,Douds D,Osman SF,Doner LW,Ratcliffe RG

    更新日期:1995-05-01 00:00:00

  • Effects of Nitrate Application on Amaranthus powellii Wats : II. Stomatal Response to Vapor Pressure Difference is Consistent with Optimization of Stomatal Conductance.

    abstract::The hypothesis of optimal stomatal conductance predicts conductance should vary with changes of the vapor pressure difference between leaf and air (VPD) to keep the partial derivative of transpiration rate (E) with respect to assimilation rate (A) constant ( partial differentialE/ partial differentialA = lambda). Stom...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.3.614

    authors: Hunt ER,Weber JA,Gates DM

    更新日期:1985-11-01 00:00:00

  • Global patterns of gene expression in the aleurone of wild-type and dwarf1 mutant rice.

    abstract::The cereal aleurone layer is a model system for studying the regulation of transcription by gibberellin (GA) and abscisic acid (ABA). GA stimulates and ABA prevents the transcription of genes for alpha-amylases and other secreted hydrolytic enzymes, but how GA and ABA affect the transcription of other genes is largely...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.074435

    authors: Bethke PC,Hwang YS,Zhu T,Jones RL

    更新日期:2006-02-01 00:00:00

  • Light-dependent Influx and Efflux of Potassium of Guard Cells during Stomatal Opening and Closing.

    abstract::Stomata in epidermal strips of Vicia faba opened in light and closed in darkness when floated on dilute K(+) solutions. Opening and closing, respectively, paralleled the fluxes of labeled K(+) into and out of the strips. The gain and loss of K(+) by the strips were shown by colbaltinitrite stain to be centered at guar...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.46.3.483

    authors: Humble GD,Hsiao TC

    更新日期:1970-09-01 00:00:00

  • Photocontrol of the Accumulation of Plastid Polypeptides during Greening of Tomato Cotyledons : Potentiation by a Pulse of Red Light.

    abstract::A pulse of red light acting through phytochrome accelerates the formation of chlorophyll upon subsequent transfer of dark-grown seedlings to continuous white light. Specific antibodies were used to follow the accumulation of representative subunits of the major photosynthetic complexes during greening of seedlings of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.4.1934

    authors: Pauncz Y,Gepstein S,Horwitz BA

    更新日期:1992-12-01 00:00:00

  • Root respiration associated with nitrogenase activity (c(2)h(2)) of soybean, and a comparison of estimates.

    abstract::Root respiration associated with symbiotic fixation in soybean (Glycine max [L.] Merr.) was estimated by four methods.Averaged over the life of the plant, the root respires 5.8 milligrams C per milligram N accumulated from fixation. When nitrogenase (C(2)H(2)) activity and root respiration were decreased by treating r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.3.701

    authors: Patterson TG,Larue TA

    更新日期:1983-07-01 00:00:00

  • Sucrose Synthase Expression during Cold Acclimation in Wheat.

    abstract::When wheat (Triticum aestivum) seedlings are exposed to a cold temperature (2-4 degrees C) above 0 degrees C, sucrose accumulates and sucrose synthase activity increases. The effect of a cold period on the level of sucrose synthase (SS) was investigated. Using antibodies against wheat germ SS, Western blots studies sh...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.3.887

    authors: Crespi MD,Zabaleta EJ,Pontis HG,Salerno GL

    更新日期:1991-07-01 00:00:00

  • The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro

    abstract::The effects of plant hormones and sucrose (Suc) on potato (Solanum tuberosum L.) tuberization were studied using in vitro cultured single-node cuttings. Tuber-inducing (high Suc) and -noninducing (low Suc or high Suc plus gibberellin [GA]) media were tested. Tuberization frequencies, tuber widths, and stolon lengths w...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.2.575

    authors: Xu X,van Lammeren AA,Vermeer E,Vreugdenhil D

    更新日期:1998-06-01 00:00:00

  • Phosphorylation of the amino terminus of maize sucrose synthase in relation to membrane association and enzyme activity.

    abstract::Sucrose synthase (SUS) is phosphorylated on a major, amino-terminal site located at Ser-15 (S15) in the maize (Zea mays) SUS1 protein. Site- and phospho-specific antibodies against a phosphorylated S15 (pS15) peptide allowed direct analysis of S15 phosphorylation in relation to membrane association. Immunoblots of the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.036780

    authors: Hardin SC,Winter H,Huber SC

    更新日期:2004-04-01 00:00:00

  • Sequences downstream of the translation initiation codon are important determinants of translation efficiency in chloroplasts.

    abstract::The objective of this study was to determine if mRNA sequences downstream of the translation initiation codon are important for translation of plastid mRNAs. We have employed a transgenic approach, measuring accumulation of the neomycin phosphotransferase (NPTII) reporter enzyme translationally fused with 14 N-termina...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.125.1.430

    authors: Kuroda H,Maliga P

    更新日期:2001-01-01 00:00:00

  • Abundantly and rarely expressed Lhc protein genes exhibit distinct regulation patterns in plants.

    abstract::We have analyzed gene regulation of the Lhc supergene family in poplar (Populus spp.) and Arabidopsis (Arabidopsis thaliana) using digital expression profiling. Multivariate analysis of the tissue-specific, environmental, and developmental Lhc expression patterns in Arabidopsis and poplar was employed to characterize ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.073304

    authors: Klimmek F,Sjödin A,Noutsos C,Leister D,Jansson S

    更新日期:2006-03-01 00:00:00

  • Growth and N Allocation in Rice Plants under CO2 Enrichment.

    abstract::The effects of CO2 enrichment on growth and N allocation of rice (Oryza sativa L.) were examined. The plants were grown hydroponically in growth chambers with a 14-h photoperiod (1000 [mu]mol quanta m-2 s-1) and a day/night temperature of 25/20[deg]C. From the 28th to 70th d after germination, the plants were exposed ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.115.1.199

    authors: Makino A,Harada M,Sato T,Nakano H,Mae T

    更新日期:1997-09-01 00:00:00