Organelle-bound malate dehydrogenase isoenzymes are synthesized as higher molecular weight precursors.

Abstract:

:Biosynthesis of malate dehydrogenase isoenzymes was studied in cotyledons of watermelons (Citrullus vulgaris Schrad., var. Stone Mountain). The glyoxysomal and mitochondrial isoenzymes are synthesized as higher molecular weight precursors which can be immunoprecipitated by mono-specific antibodies from the products of in vitro translation in reticulocyte lysates programed with cotyledonary mRNA and with the same size from enzyme extracts of pulse-labeled cotyledons. During translocation from the cytosol into the organelles, processing takes place. An 8 kilodalton extra sequence is cleaved from the glyoxysomal precursor and a 3.3 kilodalton extra sequence from the mitochondrial precursor producing the native subunits of 33 and 38 kilodaltons, respectively. The data support a post-translational translocation of the organelle-destined malate dehydrogenase isoenzymes. The in vitro translation of the cytosolic malate dehydrogenase I yields a product which has the same molecular weight as the subunit of the native isoenzyme (39.5 kilodaltons).

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Gietl C,Hock B

doi

10.1104/pp.70.2.483

subject

Has Abstract

pub_date

1982-08-01 00:00:00

pages

483-7

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

70

pub_type

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