Metabolic modeling identifies key constraints on an engineered glycine betaine synthesis pathway in tobacco.

abstract：:A procedure was developed to separate and partially purify two NAD(P)H dehydrogenases from the inner membrane of cauliflower (Brassica oleracea L.) mitochondria. The procedure used Triton X-100 extraction followed by (NH(4))(2)SO(4) precipitation and gel filtration (Sepharose G-200 column) chromatography. The first de...

journal_title：Plant physiology

authors： Klein RR,Burke JJ

更新日期：1984-10-01 00:00:00

abstract：:The anaphase promoting complex or cyclosome is the ubiquitin-ligase that targets destruction box-containing proteins for proteolysis during the cell cycle. Anaphase promoting complex or cyclosome and its activator (the fizzy and fizzy-related) proteins work together with ubiquitin-conjugating enzymes (UBCs) (E2s). One...

journal_title：Plant physiology

authors： Criqui MC,de Almeida Engler J,Camasses A,Capron A,Parmentier Y,Inzé D,Genschik P

更新日期：2002-11-01 00:00:00

abstract：:The trans-Golgi network (TGN) plays a central role in cellular secretion and has been implicated in sorting cargo destined for the plasma membrane. Previously, the Arabidopsis (Arabidopsis thaliana) echidna (ech) mutant was shown to exhibit a dwarf phenotype due to impaired cell expansion. However, ech also has a prev...

journal_title：Plant physiology

authors： Fan X,Yang C,Klisch D,Ferguson A,Bhaellero RP,Niu X,Wilson ZA

更新日期：2014-03-01 00:00:00

abstract：:Plants of Pisum sativum L. ;Alsweet' were grown under a controlled environment and exposed to SO(2) and O(3) to determine whether changes in stomatal aperture during exposure were related to subsequent leaf injury. Stomata consistently closed with injurious levels of SO(2) and O(3). Measurements with diffusion poromet...

journal_title：Plant physiology

authors： Olszyk DM,Tibbitts TW

更新日期：1981-03-01 00:00:00

abstract：:A procedure is described for isolating and purifying mesophyll protoplasts and bundle sheath protoplasts of the C(4) plant Panicum miliaceum. Following enzymic digestion of leaf tissue, mesophyll protoplasts and bundle sheath protoplasts are released and purified by density centrifugation. The lower density of mesophy...

journal_title：Plant physiology

authors： Edwards GE

更新日期：1979-05-01 00:00:00

abstract：:Histidine (His) is an essential ingredient for protein synthesis and is required by all living organisms. In higher plants, although there is considerable evidence that His is essential for plant growth and survival, there is very little information as to whether it plays any specific role in plant development. Here, ...

journal_title：Plant physiology

authors： Mo X,Zhu Q,Li X,Li J,Zeng Q,Rong H,Zhang H,Wu P

更新日期：2006-08-01 00:00:00

abstract：:During a 36-hour period, the rate of absorption of oxygen, respiration quotient, and rate of evolution of heat have been measured using seeds of Prosopis juliflora, Kochia scoparia, and Oxyria digyna. The results obtained in pure water were compared with those obtained in dilute solutions of dinitrolphenol. The data a...

journal_title：Plant physiology

authors： Lacher JR,Amador A,Snow K

更新日期：1966-11-01 00:00:00

abstract：:Assessing the genetic variability of plant performance under heat and drought scenarios can contribute to reduce the negative effects of climate change. We propose here an approach that consisted of (1) clustering time courses of environmental variables simulated by a crop model in current (35 years × 55 sites) and fu...

journal_title：Plant physiology

authors： Millet EJ,Welcker C,Kruijer W,Negro S,Coupel-Ledru A,Nicolas SD,Laborde J,Bauland C,Praud S,Ranc N,Presterl T,Tuberosa R,Bedo Z,Draye X,Usadel B,Charcosset A,Van Eeuwijk F,Tardieu F

更新日期：2016-10-01 00:00:00

abstract：:Steady state rates of NADP reduction decline upon commencement of nitrite reduction in reconstituted chloroplast preparations. Similarly, steady state rates of nitrite reduction are lower, but not zero, during concurrent NADP reduction. These results imply that competition for substrate occurs and suggest that nitrite...

journal_title：Plant physiology

authors： Baysdorfer C,Robinson JM

更新日期：1985-02-01 00:00:00

abstract：:The seeds of lettuce (Lactuca sativa L. cv. Grand Rapids) germinate in darkness at 25 C when treated by gibberellic acid (GA(3)) for 1 hour following 2 hours of imbibition. The time of GA(3) application influences the rate and the final percentage of seeds that germinate. In contrast, red light illumination given at d...

journal_title：Plant physiology

authors： Lewak S,Khan AA

更新日期：1977-10-01 00:00:00

abstract：:Five major fatty acids, palmitic (16:0), stearic (18:0), oleic (18:1), linoleic (18:2), and linolenic (18:3), were identified in polar lipid extracts from pulvini of Samanea saman and Phaseolus coccineus. In P. coccineus their distribution varied quantitatively in the laminar pulvinus, petiolar pulvinus, petiole, stem...

journal_title：Plant physiology

authors： Gardner GF,Stowe BB

更新日期：1979-09-01 00:00:00

abstract：:A 3,300-bp DNA fragment encoding the carboxyl-transferase domain of the multidomain, chloroplastic acetyl-coenzyme A carboxylase (ACCase) was sequenced in aryloxyphenoxypropionate (APP)-resistant and -sensitive Alopecurus myosuroides (Huds.). No resistant plant contained an Ile-1,781-Leu substitution, previously shown...

journal_title：Plant physiology

authors： Délye C,Zhang XQ,Chalopin C,Michel S,Powles SB

更新日期：2003-07-01 00:00:00

abstract：:The existence of sphingolipid- and sterol-enriched microdomains, known as lipid rafts, in the plasma membrane (PM) of eukaryotic cells is well documented. To obtain more insight into the lipid molecular species required for the formation of microdomains in plants, we have isolated detergent (Triton X-100)-resistant me...

journal_title：Plant physiology

authors： Laloi M,Perret AM,Chatre L,Melser S,Cantrel C,Vaultier MN,Zachowski A,Bathany K,Schmitter JM,Vallet M,Lessire R,Hartmann MA,Moreau P

更新日期：2007-01-01 00:00:00

abstract：:The effect of abscisic acid (ABA) on the exudation process in excised sunflower root was investigated. A promotion of both ion flux and volume exudation rate was observed. Cutting off the external supply of ions to the roots markedly increased the magnitude of the ABA effect. The promotive effect of ABA on exudation r...

journal_title：Plant physiology

authors： Glinka Z

更新日期：1980-03-01 00:00:00

abstract：:Plant growth and development are strongly dependent on sink-source interactions. In the majority of plants, sucrose (Suc) is the dominant form in which photo-assimilate is transported from source to sinks. Although the effects of Suc on photosynthetic metabolism have been intensively studied, the effect of Suc supply ...

journal_title：Plant physiology

authors： Roessner-Tunali U,Urbanczyk-Wochniak E,Czechowski T,Kolbe A,Willmitzer L,Fernie AR

更新日期：2003-10-01 00:00:00

abstract：:The alternative oxidase (AOX) pathway of plant mitochondria uncouples respiration from mitochondrial ATP production and may ameliorate plant performance under stressful environmental conditions, such as cold temperatures, by preventing excess accumulation of reactive oxygen species. We tested this model in whole tissu...

journal_title：Plant physiology

authors： Fiorani F,Umbach AL,Siedow JN

更新日期：2005-12-01 00:00:00

abstract：:A new ultraviolet (UV)-A/B absorbing pigment with maxima at 312 and 330 nanometers from the cosmopolitan terrestrial cyanobacterium Nostoc commune is described. The pigment is found in high amounts (up to 10% of dry weight) in colonies grown under solar UV radiation but only in low concentrations in laboratory culture...

journal_title：Plant physiology

authors： Scherer S,Chen TW,Böger P

更新日期：1988-12-01 00:00:00

abstract：:The halotolerant alga Dunaliella bardawil accumulates very large amounts of [beta]-carotene when exposed to high light intensity. The accumulated [beta]-carotene is concentrated in small, oily globules within the chloroplast and has been suggested to protect the alga against photodamage by high irradiation (A. Ben-Amo...

journal_title：Plant physiology

authors： Katz A,Jimenez C,Pick U

更新日期：1995-08-01 00:00:00

abstract：:A mutant of Arabidopsis thaliana has been isolated in which ribulose-1,5-bisphosphate carboxylase is present in a nonactivatable form in vivo. The mutation appears to affect carboxylase activation specifically, and not any other enzyme of the photosynthesis or photorespiratory cycles. The effect of the mutation on car...

journal_title：Plant physiology

authors： Somerville CR,Portis AR,Ogren WL

更新日期：1982-08-01 00:00:00

abstract：:ATP:citrate lyase (ACL) catalyzes the conversion of citrate to acetyl-coenzyme A (CoA) and oxaloacetate and is a key enzyme for lipid accumulation in mammals and oleaginous yeasts and fungi. To investigate whether heterologous ACL genes can be targeted and imported into the plastids of plants, a gene encoding a fusion...

journal_title：Plant physiology

authors： Rangasamy D,Ratledge C

更新日期：2000-04-01 00:00:00

abstract：:When wheat (Triticum aestivum) seedlings are exposed to a cold temperature (2-4 degrees C) above 0 degrees C, sucrose accumulates and sucrose synthase activity increases. The effect of a cold period on the level of sucrose synthase (SS) was investigated. Using antibodies against wheat germ SS, Western blots studies sh...

journal_title：Plant physiology

authors： Crespi MD,Zabaleta EJ,Pontis HG,Salerno GL

更新日期：1991-07-01 00:00:00

abstract：:The triphosphate tunnel metalloenzyme (TTM) superfamily comprises a group of enzymes that hydrolyze organophosphate substrates. They exist in all domains of life, yet the biological role of most family members is unclear. Arabidopsis (Arabidopsis thaliana) encodes three TTM genes. We have previously reported that AtTT...

journal_title：Plant physiology

authors： Ung H,Karia P,Ebine K,Ueda T,Yoshioka K,Moeder W

更新日期：2017-09-01 00:00:00

abstract：:We analyzed lignin content and composition in transgenic tobacco (Nicotiana tabacum) lines altered in the expression of the early phenylpropanoid biosynthetic enzymes L-phenylalanine ammonia-lyase and cinnamate 4-hydroxylase (C4H). The reduction of C4H activity by antisense expression or sense suppression resulted in ...

journal_title：Plant physiology

authors： Sewalt V,Ni W,Blount JW,Jung HG,Masoud SA,Howles PA,Lamb C,Dixon RA

更新日期：1997-09-01 00:00:00

abstract：:Endosperm halves from 3-day-old castor bean (Ricinus communis var Hale) were incubated for 30 minutes with l-[(14)C]serine, after which label was observed in ethanolamine, choline, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine, ethanolaminephosphate, and CDPethanolamine, but not in cholinephosphate...

journal_title：Plant physiology

authors： Kinney AJ,Moore TS

更新日期：1987-05-01 00:00:00

abstract：:Cryptogein (CRY), a protein secreted by Phytophthora cryptogea, causes necrosis on tobacco (Nicotiana tabacum) plants at the site of application (the stem or the roots) and also on distant leaves. Autoradiography of plantlets after root absorption of radioiodinated CRY demonstrated a rapid migration of the label to th...

journal_title：Plant physiology

authors： Devergne JC,Bonnet P,Panabières F,Blein JP,Ricci P

更新日期：1992-07-01 00:00:00

abstract：:Plant cystatins, similar to other defense proteins, include hypervariable, positively selected amino acid sites presumably impacting their biological activity. Using 29 single mutants of the eighth domain of tomato (Solanum lycopersicum) multicystatin, SlCYS8, we assessed here the potential of site-directed mutagenesi...

journal_title：Plant physiology

authors： Goulet MC,Dallaire C,Vaillancourt LP,Khalf M,Badri AM,Preradov A,Duceppe MO,Goulet C,Cloutier C,Michaud D

更新日期：2008-03-01 00:00:00

abstract：:The (13)C nuclear magnetic resonance spectra of sucrose extracted from intact soybean and corn leaves labeled with (13)CO(2) for 5 to 180 minutes have been obtained at 22.6 megahertz. The spectra provide a direct determination of the relative concentrations of (12)C-(13)C and (13)C-(13)C pairs of carbons in the labele...

journal_title：Plant physiology

authors： Schaefer J,Kier LD,Stejskal EO

更新日期：1980-02-01 00:00:00

abstract：:ADP-glucose pyrophosphorylase (AGPase) is a key regulatory enzyme in starch biosynthesis. However, plant AGPases differ in several parameters, including spatial and temporal expression, allosteric regulation, and heat stability. AGPases of cereal endosperms are heat labile, while those in other tissues, such as the po...

journal_title：Plant physiology

authors： Linebarger CR,Boehlein SK,Sewell AK,Shaw J,Hannah LC

更新日期：2005-12-01 00:00:00

abstract：:To determine the selectivity of movement of amino acids from source leaves to sink tissues in soybeans (Glycine max [L.] Merr. ;Wells'), (14)C-labeled serine, leucine, or lysine was applied to an abraded spot on a fully expanded trifoliolate leaflet, and an immature sink leaf three nodes above was monitored with a GM ...

journal_title：Plant physiology

authors： Housley TL,Peterson DM,Schrader LE

更新日期：1977-02-01 00:00:00

abstract：:Calcium phosphate-boric acid treatments and UDP-glucose both elicited aniline blue fluorescent, periodic acid-Schiff's reagent-resistant, deposits in association with the cell walls of cowpea (Vigna sinensis [Torner] Savi cv. Early Ramshorn) tissue. Those deposits induced by calcium phosphateboric acid treatment ultra...

journal_title：Plant physiology

authors： Tighe DM,Heath MC

更新日期：1982-02-01 00:00:00