Reduced Lignin Content and Altered Lignin Composition in Transgenic Tobacco Down-Regulated in Expression of L-Phenylalanine Ammonia-Lyase or Cinnamate 4-Hydroxylase.

Abstract:

:We analyzed lignin content and composition in transgenic tobacco (Nicotiana tabacum) lines altered in the expression of the early phenylpropanoid biosynthetic enzymes L-phenylalanine ammonia-lyase and cinnamate 4-hydroxylase (C4H). The reduction of C4H activity by antisense expression or sense suppression resulted in reduced levels of Klason lignin, accompanied by a decreased syringyl/guaiacyl monomer ratio as determined by pyrolysis gas chromatography/mass spectrometry Similar reduction of lignin levels by down -regulation of L-phenylalanine ammonia-lyase, the enzyme preceding C4H in the central phenylpropanoid pathway, did not result in a decreased syringyl/guaiacyl ratio. Rather, analysis of lignin methoxyl content and pyrolysis suggested an increased syringyl/guaiacyl ratio. One possible explanation of these results is that monolignol biosynthesis from L-phenylalanine might occur by more than one route, even at the early stages of the core phenylpropanoid pathway, prior to the formation of specific monolignol precursors.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Sewalt V,Ni W,Blount JW,Jung HG,Masoud SA,Howles PA,Lamb C,Dixon RA

doi

10.1104/pp.115.1.41

subject

Has Abstract

pub_date

1997-09-01 00:00:00

pages

41-50

issue

1

eissn

0032-0889

issn

1532-2548

pii

115/1/41

journal_volume

115

pub_type

杂志文章
  • Ectopic expression of an amino acid transporter (VfAAP1) in seeds of Vicia narbonensis and pea increases storage proteins.

    abstract::Storage protein synthesis is dependent on available nitrogen in the seed, which may be controlled by amino acid import via specific transporters. To analyze their rate-limiting role for seed protein synthesis, a Vicia faba amino acid permease, VfAAP1, has been ectopically expressed in pea (Pisum sativum) and Vicia nar...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.056523

    authors: Rolletschek H,Hosein F,Miranda M,Heim U,Götz KP,Schlereth A,Borisjuk L,Saalbach I,Wobus U,Weber H

    更新日期:2005-04-01 00:00:00

  • The protein kinase SnRK2.6 mediates the regulation of sucrose metabolism and plant growth in Arabidopsis.

    abstract::In higher plants, three subfamilies of sucrose nonfermenting-1 (Snf1)-related protein kinases have evolved. While the Snf1-related protein kinase 1 (SnRK1) subfamily has been shown to share pivotal roles with the orthologous yeast Snf1 and mammalian AMP-activated protein kinase in modulating energy and metabolic homeo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.150789

    authors: Zheng Z,Xu X,Crosley RA,Greenwalt SA,Sun Y,Blakeslee B,Wang L,Ni W,Sopko MS,Yao C,Yau K,Burton S,Zhuang M,McCaskill DG,Gachotte D,Thompson M,Greene TW

    更新日期:2010-05-01 00:00:00

  • Effect of water stress on the reduction of nitrate and nitrite by soybean nodules.

    abstract::The effects of water stress on nitrate reductase and nitrite reductase activities in symbiotic nodules were examined in field-grown soybean plants (Glycine max L Merr. cv Clark). The in vitro assays of enzyme activity indicated that the nodule cytosol and bacteroids contained both nitrate reductase and nitrite reducta...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.4.1065

    authors: Chen CL,Sung JM

    更新日期:1983-12-01 00:00:00

  • Regulatory Phosphorylation of C4 Phosphoenolpyruvate Carboxylase (A Cardinal Event Influencing the Photosynthesis Rate in Sorghum and Maize).

    abstract::C4 leaf phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) is subject to a day/night regulatory phosphorylation cycle. By using the cytoplasmic protein synthesis inhibitor cycloheximide (CHX), we previously reported that the reversible in vivo light activation of the C4 PEPC protein-serine kinase requires protein syn...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.3.891

    authors: Bakrim N,Prioul JL,Deleens E,Rocher JP,Arrio-Dupont M,Vidal J,Gadal P,Chollet R

    更新日期:1993-03-01 00:00:00

  • Assay of Proton-Coupled Glycolate and D-Glycerate Transport into Chloroplast Inner Envelope Membrane Vesicles by Stopped-Flow Fluorescence.

    abstract::The transport of glycolate and D-glycerate across the inner envelope membrane of intact chloroplasts is rapid and mediated by a translocator with proton/substrate symport activity. The true initial rate of glycolate or D-glycerate transport could not be measured by conventional methods. To resolve the initial rates of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.3.793

    authors: Young XK,McCarty RE

    更新日期:1993-03-01 00:00:00

  • Effects of drought on primary photosynthetic processes of cotton leaves.

    abstract::The effects of drought on Photosystem II (PSII) fluorescence and photosynthetic electron transport activities were analyzed in cotton. Water stress did not modify the amplitude of leaf variable fluorescence at room temperature in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) nor at 77 K. It is therefo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.2.360

    authors: Genty B,Briantais JM,Da Silva JB

    更新日期:1987-02-01 00:00:00

  • Computer Simulation Model for the Biosynthesis of Galactosyldiacylglycerols and Fatty Acid Desaturation in Plants (Determination of Rates of Desaturase Activity in Monogalactosyldiacylglycerol).

    abstract::The level of unsaturation of the constituent fatty acids of many glycerolipids in plant membranes is modified by environmental factors. The measurement of the rate of the desaturation of these fatty acids is essential to an understanding of how plants adapt to changing environments. This is difficult because of the co...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.3.977

    authors: Williams JP,Khan MU,Wong D

    更新日期:1993-03-01 00:00:00

  • Sensitivity to an Ethylene Biosynthesis-Inducing Endoxylanase in Nicotiana tabacum L. cv Xanthi Is Controlled by a Single Dominant Gene.

    abstract::The ethylene biosynthesis-inducing xylanase (EIX) is known to be a potent elicitor of ethylene biosynthesis and other responses when applied to leaf tissue of Nicotiana tabacum L. cv Xanthi. In contrast, leaf tissue of the tobacco cultivar Hicks was insensitive to EIX at concentrations 100-fold higher than was needed ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.3.1081

    authors: Bailey BA,Korcak RF,Anderson JD

    更新日期:1993-03-01 00:00:00

  • Involvement of Abscisic Acid in Ethylene-Induced Cotyledon Abscission in Cotton Seedlings.

    abstract::Cotton (Gossypium hirsutum L. cv LG102) seedlings raised from seeds exposed to 100 [mu]M norflurazon (NFZ) during imbibition contained reduced levels of free abscisic acid (ABA) and were visibly achlorophyllous. Exposure of untreated cotton seedlings to ethylene concentrations >1 [mu]L/L for 24 h resulted in cotyledon...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.2.641

    authors: Suttle JC,Hultstrand JF

    更新日期:1993-02-01 00:00:00

  • Synthesis of chymotrypsin inhibitor I protein in potato leaflets induced by detachment.

    abstract::Chymotrypsin inhibitor I is a protein that can be induced to accumulate in potato leaflets within hr in the light when leaflets are detached from intact plants and supplied with water. This increase in inhibitor I is not accompanied by an increase in all proteins in the detached leaflets. The accumulation is polar, pr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.11.1859

    authors: Ryan CA

    更新日期:1968-11-01 00:00:00

  • Changes in the Content of Two Glutamate Synthase Proteins in Spikelets of Rice (Oryza sativa) Plants during Ripening.

    abstract::Nitrogen accumulation in the apical spikelets on the primary branches of the main stem of rice plants have been studied during the ripening process (0-35 d after flowering). The level of NADH-dependent glutamate synthase (GOGAT) protein and activity increased 4- and 6-fold, respectively, in the first 15 d after flower...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.4.1257

    authors: Hayakawa T,Yamaya T,Mae T,Ojima K

    更新日期:1993-04-01 00:00:00

  • MtSWEET11, a Nodule-Specific Sucrose Transporter of Medicago truncatula.

    abstract::Optimization of nitrogen fixation by rhizobia in legumes is a key area of research for sustainable agriculture. Symbiotic nitrogen fixation (SNF) occurs in specialized organs called nodules and depends on a steady supply of carbon to both plant and bacterial cells. Here we report the functional characterization of a n...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.01910

    authors: Kryvoruchko IS,Sinharoy S,Torres-Jerez I,Sosso D,Pislariu CI,Guan D,Murray J,Benedito VA,Frommer WB,Udvardi MK

    更新日期:2016-05-01 00:00:00

  • The mechanism of iron homeostasis in the unicellular cyanobacterium synechocystis sp. PCC 6803 and its relationship to oxidative stress.

    abstract::In this article, we demonstrate the connection between intracellular iron storage and oxidative stress response in cyanobacteria. Iron is essential for the survival of all organisms. However, the redox properties that make iron a valuable cofactor also lead to oxidative interactions, resulting in the formation of harm...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.141853

    authors: Shcolnick S,Summerfield TC,Reytman L,Sherman LA,Keren N

    更新日期:2009-08-01 00:00:00

  • Protein and RNA Synthesizing Activities during Sexual Process of Heterothallic Closterium.

    abstract::Protein and RNA synthesizing activities increased markedly during the mating process and decreased during the maturation stage of zygotes in heterothallic strains of Closterium peracerosum-strigosum-littoale, KAS-4-29 (mating-type minus) and KAS -4-30 (mating-type plus) and a homothallic Closterium acerosum. Different...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.4.696

    authors: Kato A,Sasaki K

    更新日期:1985-08-01 00:00:00

  • GROWTH REGULATING FACTOR5 stimulates Arabidopsis chloroplast division, photosynthesis, and leaf longevity.

    abstract::Arabidopsis (Arabidopsis thaliana) leaf development relies on subsequent phases of cell proliferation and cell expansion. During the proliferation phase, chloroplasts need to divide extensively, and during the transition from cell proliferation to expansion, they differentiate into photosynthetically active chloroplas...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.256180

    authors: Vercruyssen L,Tognetti VB,Gonzalez N,Van Dingenen J,De Milde L,Bielach A,De Rycke R,Van Breusegem F,Inzé D

    更新日期:2015-03-01 00:00:00

  • Preadaptation of protein synthesis in wheat seedlings to high temperature.

    abstract::The optimum temperature of protein synthesis in wheat seedlings (Triticum aestivum L.), measured as (14)C-leucine incorporation, depends on the growing temperature. Plants grown at reduced temperature (4 C) reach their optimum at 27.5 C, whereas plants kept at 36 C have the highest rate of protein synthesis at 35 C. T...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.56.5.590

    authors: Weidner M,Ziemens C

    更新日期:1975-11-01 00:00:00

  • Environmental Parameters Affecting Dark Response of Rice Seedlings (Oryza sativa L.) to Triacontanol.

    abstract::Triacontanol applied to IR-8 rice (Oryza sativa L.) seedlings in nutrient solution caused an increase in dry weight during a 6-hour dark period. This increase was altered by atmospheric CO(2) and O(2) concentrations. The largest growth response occurred from 200 to 350 muliters/liter CO(2) with 5% O(2). The treated se...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.5.851

    authors: Bittenbender HC,Dilley DR,Wert V,Ries SK

    更新日期:1978-05-01 00:00:00

  • Release of Photosynthetic Protein Catabolites by Blebbing from Thylakoids.

    abstract::Thylakoid proteins and their catabolites have been detected in lipid-protein particles isolated from the stroma of intact chloroplasts obtained from primary leaves of 2-week-old bean seedlings (Phaseolus vulgaris L. cv Kinghorn). The lipid-protein particles bear morphological resemblance to plastoglobuli seen in the c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.4.1547

    authors: Ghosh S,Hudak KA,Dumbroff EB,Thompson JE

    更新日期:1994-12-01 00:00:00

  • Chloroplastic phosphoadenosine phosphosulfate metabolism regulates basal levels of the prohormone jasmonic acid in Arabidopsis leaves.

    abstract::Levels of the enzymes that produce wound response mediators have to be controlled tightly in unwounded tissues. The Arabidopsis (Arabidopsis thaliana) fatty acid oxygenation up-regulated8 (fou8) mutant catalyzes high rates of alpha -linolenic acid oxygenation and has higher than wild-type levels of the alpha -linoleni...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.150474

    authors: Rodríguez VM,Chételat A,Majcherczyk P,Farmer EE

    更新日期:2010-03-01 00:00:00

  • Biochemical factors affecting protein accumulation in the rice grain.

    abstract::Rice grains (Oryza sativa L.) from three varieties and three pairs of lines with different protein content were collected at 4-day intervals from 4 to 32 days after flowering. The samples were analyzed for protein, free amino nitrogen, ribonucleic acid, protease activity, and ribonuclease activity. In addition, the ca...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.46.5.743

    authors: Cruz LJ,Cagampang GB,Juliano BO

    更新日期:1970-11-01 00:00:00

  • An imperfect heat shock element and different upstream sequences are required for the seed-specific expression of a small heat shock protein gene.

    abstract::Chimeric constructs containing the promoter and upstream sequences of Ha hsp17.6 G1, a small heat shock protein gene, reproduced in transgenic tobacco (Nicotiana tabacum) its unique seed-specific expression patterns previously reported in sunflower. These constructs did not respond to heat shock, but were expressed wi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.121.3.723

    authors: Carranco R,Almoguera C,Jordano J

    更新日期:1999-11-01 00:00:00

  • mRNA Localization in Plant Cells.

    abstract::Localization of mRNAs at the subcellular level is an essential mechanism for specific protein targeting and local control of protein synthesis in both eukaryotes and bacteria. While mRNA localization is well documented in metazoans, somatic cells, and microorganisms, only a handful of well-defined mRNA localization ex...

    journal_title:Plant physiology

    pub_type: 杂志文章,评审

    doi:10.1104/pp.19.00972

    authors: Tian L,Chou HL,Fukuda M,Kumamaru T,Okita TW

    更新日期:2020-01-01 00:00:00

  • The occurrence of phytoferritin and its relationship to effectiveness of soybean nodules.

    abstract::Polyacrylamide gel electrophoresis and electron microscopy revealed that accumulation of iron-protein in soybean nodules is influenced by nodule age, mutation in bradyrhizobia, and rhizobial/bradyrhizobial strain-soybean cultivar interactions. Iron-protein concentrations (micrograms per milligram protein) were inverse...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.2.299

    authors: Ko MP,Huang PY,Huang JS,Barker KR

    更新日期:1987-02-01 00:00:00

  • Sugar Potentiation of Fatty Acid and Triacylglycerol Accumulation.

    abstract::Photosynthetically derived sugar provides carbon skeletons for lipid biosynthesis. We used mutants of Arabidopsis (Arabidopsis thaliana) and the expression of oleogenic factors to investigate relationships among sugar availability, lipid synthesis, and the accumulation of triacylglycerol (TAG) in leaf tissue. The adg1...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00828

    authors: Zhai Z,Liu H,Xu C,Shanklin J

    更新日期:2017-10-01 00:00:00

  • Buoyant density studies of chloroplast and nuclear deoxyribonucleic acid from control and 3-amino-1,2,4-triazole-treated wheat seedlings, Triticum vulgare.

    abstract::The isolation of chloroplast and nuclear DNA from dark- and light-grown, control- and 3-amino-1,2,4-triazole-treated wheat seedlings, Triticum vulgare, is described. Contrary to a previous report, we found that chloroplast and nuclear DNA had similar buoyant densities (1.702 grams per cubic centimeter) and that they c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.46.6.825

    authors: Bartels PG,Hyde A

    更新日期:1970-12-01 00:00:00

  • Nitric oxide modulates the activity of tobacco aconitase.

    abstract::Recent evidence suggests an important role for nitric oxide (NO) signaling in plant-pathogen interactions. Additional elucidation of the role of NO in plants will require identification of NO targets. Since aconitases are major NO targets in animals, we examined the effect of NO on tobacco (Nicotiana tabacum) aconitas...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.2.573

    authors: Navarre DA,Wendehenne D,Durner J,Noad R,Klessig DF

    更新日期:2000-02-01 00:00:00

  • Loss of tomato cell wall galactan may involve reduced rate of synthesis.

    abstract::Changes in the galactose content of the noncellulosic polysaccharides of tomato (Mill) fruit cell walls were analyzed under various conditions. On the plant, galactan decreased gradually during fruit growth. As normal fruits ripened, the loss of galactan increased sharply; this was not observed in attached rin fruits ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.3.532

    authors: Lackey GD,Gross KC,Wallner SJ

    更新日期:1980-09-01 00:00:00

  • Temperature dependence of the concentration kinetics of absorption of phosphate and potassium in corn roots.

    abstract::The effect of temperature on respiration and kinetics of H(2)PO(4) (-) and K(+) uptake in corn roots was determined in the range of 2 to 42 C. The response of uptake to temperature, determined from Q(10) and activation energy (Ea) data, for the anion and the cation differ significantly, especially in the range of upta...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.67.4.815

    authors: Bravo-F P,Uribe EG

    更新日期:1981-04-01 00:00:00

  • Formation of carbon monoxide and bile pigment in red and blue-green algae.

    abstract::Five blue-green and one red algal species produced carbon monoxide during photosynthetic growth. The blue-green algae synthesized CO and phycocyanobilin in equimolar quantities at identical rates. The red alga, Porphyridium cruentum, incorporated Delta-aminolevulinic acid-5-(14)C into phycoerythrobilin and CO. The rat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.51.1.72

    authors: Troxler RF,Dokos JM

    更新日期:1973-01-01 00:00:00

  • Localization of [gamma]-Glutamylcysteine Synthetase and Glutathione Synthetase Activity in Maize Seedlings.

    abstract::Fresh weight, protein, cysteine, [gamma]-glutamylcysteine, glutathione, and the extractable activity of the enzymes of glutathione biosynthesis, [gamma]-glutamylcysteine synthetase (EC 6.3.2.2) and glutathione synthetase (EC 6.3.2.3), were measured in roots, scutella, endosperms, and shoots of 3-, 7-, and 11-d-old mai...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.2.561

    authors: Ruegsegger A,Brunold C

    更新日期:1993-02-01 00:00:00