Abstract:
:We analyzed lignin content and composition in transgenic tobacco (Nicotiana tabacum) lines altered in the expression of the early phenylpropanoid biosynthetic enzymes L-phenylalanine ammonia-lyase and cinnamate 4-hydroxylase (C4H). The reduction of C4H activity by antisense expression or sense suppression resulted in reduced levels of Klason lignin, accompanied by a decreased syringyl/guaiacyl monomer ratio as determined by pyrolysis gas chromatography/mass spectrometry Similar reduction of lignin levels by down -regulation of L-phenylalanine ammonia-lyase, the enzyme preceding C4H in the central phenylpropanoid pathway, did not result in a decreased syringyl/guaiacyl ratio. Rather, analysis of lignin methoxyl content and pyrolysis suggested an increased syringyl/guaiacyl ratio. One possible explanation of these results is that monolignol biosynthesis from L-phenylalanine might occur by more than one route, even at the early stages of the core phenylpropanoid pathway, prior to the formation of specific monolignol precursors.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Sewalt V,Ni W,Blount JW,Jung HG,Masoud SA,Howles PA,Lamb C,Dixon RAdoi
10.1104/pp.115.1.41subject
Has Abstractpub_date
1997-09-01 00:00:00pages
41-50issue
1eissn
0032-0889issn
1532-2548pii
115/1/41journal_volume
115pub_type
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