Effect of water stress on the reduction of nitrate and nitrite by soybean nodules.

Abstract:

:The effects of water stress on nitrate reductase and nitrite reductase activities in symbiotic nodules were examined in field-grown soybean plants (Glycine max L Merr. cv Clark). The in vitro assays of enzyme activity indicated that the nodule cytosol and bacteroids contained both nitrate reductase and nitrite reductase activities. The reduction of nitrate in bacteroids increased significantly as nodule water potential declined from -0.6 to -1.4 megapascals, and then decreased when -1.8 megapascals water potential was reached. On the contrary, the reduction of nitrate in nodule cytosol was inhibited as water stress progressed. Increases in water stress intensity also caused a significant inhibition in nitrite reductase activities of bacteroids and nodule cytosol within soybean nodules. The results show that nitrate reduction occurred both in the cytosol and bacteroids of water-stressed soybean nodules. The reduction of nitrate functioned at different physiological modes in these two fractions.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Chen CL,Sung JM

doi

10.1104/pp.73.4.1065

subject

Has Abstract

pub_date

1983-12-01 00:00:00

pages

1065-6

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

73

pub_type

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