DIACYLGLYCEROL ACYLTRANSFERASE and DIACYLGLYCEROL KINASE Modulate Triacylglycerol and Phosphatidic Acid Production in the Plant Response to Freezing Stress.

Abstract:

:Plants accumulate the lipids phosphatidic acid (PA), diacylglycerol (DAG), and triacylglycerol (TAG) during cold stress, but how plants balance the levels of these lipids to mediate cold responses remains unknown. The enzymes ACYL-COENZYME A:DIACYLGLYCEROL ACYLTRANSFERASE (DGAT) and DIACYLGLYCEROL KINASE (DGK) catalyze the conversion of DAG to TAG and PA, respectively. Here, we show that DGAT1, DGK2, DGK3, and DGK5 contribute to the response to cold in Arabidopsis (Arabidopsis thaliana). With or without cold acclimation, the dgat1 mutants exhibited higher sensitivity upon freezing exposure compared with the wild type. Under cold conditions, the dgat1 mutants showed reduced expression of C-REPEAT/DRE BINDING FACTOR2 and its regulons, which are essential for the acquisition of cold tolerance. Lipid profiling revealed that freezing significantly increased the levels of PA and DAG while decreasing TAG in the rosettes of dgat1 mutant plants. During freezing stress, the accumulation of PA in dgat1 plants stimulated NADPH oxidase activity and enhanced RbohD-dependent hydrogen peroxide production compared with the wild type. Moreover, the cold-inducible transcripts of DGK2, DGK3, and DGK5 were significantly more up-regulated in the dgat1 mutants than in the wild type during cold stress. Consistent with this observation, dgk2, dgk3, and dgk5 knockout mutants showed improved tolerance and attenuated PA production in response to freezing temperatures. Our findings demonstrate that the conversion of DAG to TAG by DGAT1 is critical for plant freezing tolerance, acting by balancing TAG and PA production in Arabidopsis.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Tan WJ,Yang YC,Zhou Y,Huang LP,Xu L,Chen QF,Yu LJ,Xiao S

doi

10.1104/pp.18.00402

subject

Has Abstract

pub_date

2018-07-01 00:00:00

pages

1303-1318

issue

3

eissn

0032-0889

issn

1532-2548

pii

pp.18.00402

journal_volume

177

pub_type

杂志文章
  • The distribution of carbonic anhydrase and ribulose diphosphate carboxylase in maize leaves.

    abstract::Extraction of maize (Zea mays) leaves by progressive grinding under suitably protective conditions yields total carbonic anhydrase activities (4800 units per milligram chlorophyll) comparable to the activity in spinach (Spinacia oleracea) leaves. The total ribulose diphosphate carboxylase activity was also equal to or...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.3.336

    authors: Poincelot RP

    更新日期:1972-09-01 00:00:00

  • Beta-Amylases from Alfalfa (Medicago sativa L.) Roots.

    abstract::Amylase was found in high activity (193 international units per milligram protein) in the tap root of alfalfa (Medicago sativa L. cv. Sonora). The activity was separated by gel filtration chromatography into two fractions with molecular weights of 65,700 (heavy amylase) and 41,700 (light amylase). Activity staining of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.5.1096

    authors: Doehlert DC,Duke SH,Anderson L

    更新日期:1982-05-01 00:00:00

  • Determination of Abscisic Acid in Pinus densiflora by Selected Ion Monitoring.

    abstract::Abscisic acid (ABA) in the stem of akamatsu (Pinus densiflora) was identified and quantified by gas chromatography-mass spectrometry-selected ion monitoring using hexadeuterated ABA as an internal standard. tert-Butyldimethylsilyl ester was used as a derivative of ABA. This derivative had high sensitivity and selectiv...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.3.525

    authors: Funada R,Sugiyama T,Kubo T,Fushitani M

    更新日期:1988-11-01 00:00:00

  • Abscisic Acid and the Photoperiodic Induction of Dormancy in Salix viminalis L.

    abstract::A series of growth room experiments was carried out aiming to establish the role of abscisic acid on dormancy of Salix viminalis L. The inhibitor content and abscisic acid levels of extracts from roots, sap, leaves, and apical tissues of willow were measured using biological assay and gas-liquid chromatography.No evid...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.4.774

    authors: Alvim R,Saunders PF,Barros RS

    更新日期:1979-04-01 00:00:00

  • Water Use Efficiency of Field-grown Maize during Moisture Stress.

    abstract::Theoretical analysis of the CO(2) assimilation and water loss by single leaves suggests that the water use efficiency of C(4) species decreases as stomatal resistance increases. To confirm this hypothesis for a complete maize crop, results from computer simulations and a field experiment were compiled for varying stom...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.56.2.245

    authors: Sinclair TR,Bingham GE,Lemon ER,Allen LH

    更新日期:1975-08-01 00:00:00

  • Respiration and mitochondrial biogenesis in germinating embryos of maize.

    abstract::Function of the cyanide-sensitive mitochondrial electron transport system was required for germination of the Zea mays embryo. Respiration of the standard electron transport system (rather than the alternate oxidase) began immediately upon initiation of imbibition. This respiration depended upon cytochrome c oxidase a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.1.295

    authors: Ehrenshaft M,Brambl R

    更新日期:1990-05-01 00:00:00

  • Nickel in Plants: II. Distribution and Chemical Form in Soybean Plants.

    abstract::The gross tissue distribution, intracellular fate, and chemical behavior of Ni(2+) in soybean plants (Glycine max cv. Williams) were investigated. Following root absorption, Ni was highly mobile in the plant, with leaves being the major sink in the shoots for Ni during vegetative growth. A senescence >70% of the Ni pr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.4.566

    authors: Cataldo DA,Garland TR,Wildung RE

    更新日期:1978-10-01 00:00:00

  • Cellular distribution of polysome populations of castor bean endosperm.

    abstract::The distribution of polysomes in the cells of the endosperm of very young (48 hours of germination) seedlings of castor bean (Ricinus communis var. Hale) has been examined. Seedlings exposed to 100 micromolar gibberellin A(3) for periods up to 24 hours showed increased numbers of polysomes associated with endomembrane...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.2.359

    authors: González E

    更新日期:1980-02-01 00:00:00

  • Oxygen-18 and deuterium labeling studies of choline oxidation by spinach and sugar beet.

    abstract::Chenopods synthesize betaine by a two-step oxidation of choline: choline --> betaine aldehyde --> betaine. The pathway is chloroplastic; the first step has been shown in isolated spinach (Spinacia oleracea L.) chloroplasts to be O(2)- and light-dependent, the role of light being to provide reducing power (P Weigel, EA...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.3.695

    authors: Lerma C,Hanson AD,Rhodes D

    更新日期:1988-11-01 00:00:00

  • Alterations in Chloroplast Thylakoids during an in Vitro Freeze-Thaw Cycle.

    abstract::Plastocyanin and chloroplast coupling factor 1 (CF(1)) are released from spinach (Spinacia oleracea L.) thylakoids during a slow freezethaw cycle. CF(1) addition increases the proton uptake of thylakoids previously frozen in sucrose concentrations of 15 mm to 100 mm. Addition of CF(1) and plastocyanin restores the pro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.5.673

    authors: Garber MP,Steponkus PL

    更新日期:1976-05-01 00:00:00

  • Slow-growth phenotype of transgenic tomato expressing apoplastic invertase.

    abstract::The growth of transgenic tomato (Lycopersicon esculentum) plants that express in their apoplast yeast invertase under the control of the cauliflower mosaic virus 35S promoter is severely inhibited. The higher the level of invertase, the greater the inhibition of growth. A second phenotypic characteristic of these tran...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.2.420

    authors: Dickinson CD,Altabella T,Chrispeels MJ

    更新日期:1991-02-01 00:00:00

  • Plant protoplast agglutination by lectins.

    abstract::Concanavalin A, soybean (Glycine max L.) lectin, castor bean (Ricinus communis L.) lectin, and peanut (Arachis hypogaea L.) lectin were able to agglutinate protoplasts prepared from a variety of plant species. The seven other lectins tried were unable to agglutinate those protoplasts tested. Protoplasts prepared from ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.4.626

    authors: Larkin PJ

    更新日期:1978-04-01 00:00:00

  • Putrescine-induced wounding and its effects on membrane integrity and ion transport processes in roots of intact corn seedlings.

    abstract::Interactions between putrescine and membrane function were examined with the use of a recently developed microelectrode system that enables us simultaneously to quantify membrane potentials and net K(+) fluxes associated with individual cells at the root surface of an intact corn (Zea mays L.) seedling. In contrast to...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.3.988

    authors: Ditomaso JM,Shaff JE,Kochian LV

    更新日期:1989-07-01 00:00:00

  • Ozone-Induced Alterations in the Accumulation of Newly Synthesized Proteins in Leaves of Maize.

    abstract::We examined the response of leaves of 3-week-old maize (Zea mays L.) to short-term (5 h) fumigation with O3-enriched air (0, 0.12, 0.24, or 0.36 [mu]L/L). Older leaves and leaf tissue developed more severe visible damage at higher external O3 concentrations. To investigate the immediate effect of O3 exposure on the ac...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.2.777

    authors: Pino ME,Mudd JB,Bailey-Serres J

    更新日期:1995-06-01 00:00:00

  • Purification and Partial Characterization of a Genetically-Defined Superoxide Dismutase (SOD-1) Associated with Maize Chloroplasts.

    abstract::The chloroplast-associated form of superoxide dismutase from maize (Zea mays L.) (SOD-1) has been purified by a stepwise procedure consisting of (NH(4))(2)SO(4) fractionation, G-100 Sephadex gel filtration, DEAE-Sephacel chromatography, and hydroxylapatite chromatography. This procedure resulted in a single band on so...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.1.31

    authors: Baum JA,Chandlee JM,Scandalios JG

    更新日期:1983-09-01 00:00:00

  • Novel roles for the polyphenol oxidase enzyme in secondary metabolism and the regulation of cell death in walnut.

    abstract::The enzyme polyphenol oxidase (PPO) catalyzes the oxidation of phenolic compounds into highly reactive quinones. Polymerization of PPO-derived quinones causes the postharvest browning of cut or bruised fruit, but the native physiological functions of PPOs in undamaged, intact plant cells are not well understood. Walnu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.228593

    authors: Araji S,Grammer TA,Gertzen R,Anderson SD,Mikulic-Petkovsek M,Veberic R,Phu ML,Solar A,Leslie CA,Dandekar AM,Escobar MA

    更新日期:2014-03-01 00:00:00

  • Specificity of RCN1-mediated protein phosphatase 2A regulation in meristem organization and stress response in roots.

    abstract::Protein dephosphorylation by the serine/threonine protein phosphatase 2A (PP2A) modulates a broad array of cellular functions. PP2A normally acts as a heterotrimeric holoenzyme complex comprising a catalytic subunit bound by regulatory A and B subunits. Characterization of the regulatory A subunit isoforms (ROOTS CURL...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.112995

    authors: Blakeslee JJ,Zhou HW,Heath JT,Skottke KR,Barrios JA,Liu SY,DeLong A

    更新日期:2008-02-01 00:00:00

  • Emission of Hydrogen Sulfide by Leaf Tissue in Response to l-Cysteine.

    abstract::Leaf discs and detached leaves exposed to l-cysteine emitted a volatile sulfur compound which was proven by gas chromatography to be H(2)S. This phenomenon was demonstrated in all nine species tested (Cucumis sativus, Cucurbita pepo, Nicotiana tabacum, Coleus blumei, Beta vulgaris, Phaseolus vulgaris, Medicago sativa,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.2.430

    authors: Sekiya J,Schmidt A,Wilson LG,Filner P

    更新日期:1982-08-01 00:00:00

  • Swelling of Phaseolus mitochondria induced by the action of phospholipase A.

    abstract::Phaseolus vulgaris mitochondria incubated in sucrose swell rapidly upon the addition of phospholipase A. Bovine serum albumin inhibits the swelling. The release of free fatty acids as a result of phospholipase A action on the mitochondria is detected only in the presence of bovine serum albumin, which promotes the hyd...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.45.3.322

    authors: Earnshaw MJ,Truelove B

    更新日期:1970-03-01 00:00:00

  • Suppression of a vegetative MADS box gene of potato activates axillary meristem development.

    abstract::Potato MADS box 1 (POTM1) is a member of the SQUAMOSA-like family of plant MADS box genes isolated from an early stage tuber cDNA library. The RNA of POTM1 is most abundant in vegetative meristems of potato (Solanum tuberosum), accumulating specifically in the tunica and corpus layers of the meristem, the procambium, ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.012500

    authors: Rosin FM,Hart JK,Van Onckelen H,Hannapel DJ

    更新日期:2003-04-01 00:00:00

  • Biosynthesis of the Dihydrochalcone Sweetener Trilobatin Requires Phloretin Glycosyltransferase2.

    abstract::Epidemics of obesity and type 2 diabetes drive strong consumer interest in plant-based low-calorie sweeteners. Trilobatin is a sweetener found at high concentrations in the leaves of a range of crabapple (Malus) species, but not in domesticated apple (Malus × domestica) leaves, which contain trilobatin's bitter positi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00807

    authors: Wang Y,Yauk YK,Zhao Q,Hamiaux C,Xiao Z,Gunaseelan K,Zhang L,Tomes S,López-Girona E,Cooney J,Li H,Chagné D,Ma F,Li P,Atkinson RG

    更新日期:2020-10-01 00:00:00

  • Linear sucrose transport in protoplasts from developing soybean cotyledons.

    abstract::Previous studies with isolated soybean cotyledon protoplasts revealed the presence of a saturable, simple diffusion, and nonsaturating carrier-mediated uptake of sucrose into soybean cotyledon cells. A proton/sucrose cotransport may be involved in the saturable sucrose uptake (Lin et al. 1984 Plant Physiol 75: 936-940...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.3.649

    authors: Lin W

    更新日期:1985-07-01 00:00:00

  • Postharvest Variation in Cell Wall-Degrading Enzymes of Papaya (Carica papaya L.) during Fruit Ripening.

    abstract::Pectin methylesterase (PME), polygalacturonase (PG), xylanase, cellulase, and proteinase activity were determined and related to respiration, ethylene evolution, and changes in skin color of papaya (Carica papaya L.) fruit from harvest through to the start of fruit breakdown. PME gradually increased from the start of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.2.382

    authors: Paull RE,Chen NJ

    更新日期:1983-06-01 00:00:00

  • Contribution of malic enzyme, pyruvate kinase, phosphoenolpyruvate carboxylase, and the krebs cycle to respiration and biosynthesis and to intracellular pH regulation during hypoxia in maize root tips observed by nuclear magnetic resonance imaging and gas

    abstract::In vivo pyruvate synthesis by malic enzyme (ME) and pyruvate kinase and in vivo malate synthesis by phosphoenolpyruvate carboxylase and the Krebs cycle were measured by 13C incorporation from [1-13C]glucose into glucose-6-phosphate, alanine, glutamate, aspartate, and malate. These metabolites were isolated from maize ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.3.1073

    authors: Edwards S,Nguyen BT,Do B,Roberts JKM

    更新日期:1998-03-01 00:00:00

  • Confounding of alternate respiration by lipoxygenase activity.

    abstract::The initial burst of respiratory activity (Q(o) (2)) of imbibing soybean (Glycine max [L.] Merr. var. Wayne) seed tissue is cyanide-insensitive, and sensitive to salicylhydroxamate: presumptive evidence for the presence of alternate respiration. The initial O(2) consumption is also highly sensitive to propyl gallate. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.3.470

    authors: Parrish DJ,Leopold AC

    更新日期:1978-09-01 00:00:00

  • A Framework for Lateral Membrane Trafficking and Polar Tethering of the PEN3 ATP-Binding Cassette Transporter.

    abstract::The outermost cell layer of plants, the epidermis, and its outer (lateral) membrane domain facing the environment are continuously challenged by biotic and abiotic stresses. Therefore, the epidermis and the outer membrane domain provide important selective and protective barriers. However, only a small number of speci...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01252

    authors: Mao H,Nakamura M,Viotti C,Grebe M

    更新日期:2016-12-01 00:00:00

  • Induction of the arginine decarboxylase ADC2 gene provides evidence for the involvement of polyamines in the wound response in Arabidopsis.

    abstract::Polyamines are small ubiquitous molecules that have been involved in nearly all developmental processes, including the stress response. Nevertheless, no direct evidence of a role of polyamines in the wound response has been described. We have studied the expression of genes involved in polyamine biosynthesis in respon...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.009951

    authors: Perez-Amador MA,Leon J,Green PJ,Carbonell J

    更新日期:2002-11-01 00:00:00

  • Leaf Conductance in Relation to Assimilation in Eucalyptus pauciflora Sieb. ex Spreng: Influence of Irradiance and Partial Pressure of Carbon Dioxide.

    abstract::Rates of assimilation and transpiration in Eucalyptus pauciflora Sieb. ex Spreng were measured at various ambient partial pressures of CO(2) and various irradiances and were used to estimate leaf conductance and intercellular partial pressure of CO(2). The responses of leaf conductance and rate of assimilation to chan...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.4.670

    authors: Wong SC,Cowan IR,Farquhar GD

    更新日期:1978-10-01 00:00:00

  • The Metabolism of Oat Leaves during Senescence: II. Senescence in Leaves Attached to the Plant.

    abstract::The course of senescence in the first leaves of light-grown Avena seedlings when attached to the plant has been compared with that previously studied in detached leaves and leaf segments. Proteolysis in the leaf, whether attached or detached, is accompanied by markedly polar basipetal transport of amino acids. This po...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.6.859

    authors: Thimann KV,Tetley RR,Van Thanh T

    更新日期:1974-12-01 00:00:00

  • Effects of Air Pollutants on the Composition of Stable Carbon Isotopes, deltaC, of Leaves and Wood, and on Leaf Injury.

    abstract::Air pollutants are known to cause visible leaf injury as well as impairment of photosynthetic CO(2) fixation. Here we evaluate whether the effects on photosynthesis are large enough to cause changes in the relative composition of stable carbon isotopes, delta(13)C, of plant tissue samples, and, if so, how the changes ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.1.218

    authors: Martin B,Bytnerowicz A,Thorstenson YR

    更新日期:1988-09-01 00:00:00