Oxygen-18 and deuterium labeling studies of choline oxidation by spinach and sugar beet.

Abstract:

:Chenopods synthesize betaine by a two-step oxidation of choline: choline --> betaine aldehyde --> betaine. The pathway is chloroplastic; the first step has been shown in isolated spinach (Spinacia oleracea L.) chloroplasts to be O(2)- and light-dependent, the role of light being to provide reducing power (P Weigel, EA Weretilnyk, AD Hanson 1988 Plant Physiol 86: 54-60). Here, we report use of in vivo(18)O- and (2)H-labeling in conjunction with fast atom bombardment mass spectrometry to test for two hypothetical choline-oxidizing reactions that would explain the observed requirements for O(2) and reductant: a desaturase or an oxygenase. Simple syntheses for (2)H(3)-choline, (2)H(3), (18)O-choline, and (2)H(3), (18)O-betaine are given. A desaturase mechanism was sought by giving choline deuterated at the 2-carbon, or choline unlabeled at this position together with (2)H(2)O and by analyzing newly synthesized betaine. About 15% of the (2)H at C-2 was lost during oxidation of choline to betaine, and about 10% of the betaine made in the presence of 50% (2)H(2)O was monodeuterated. These small effects are more consistent with chemical exchange than with a desaturase, because 10 to 15% losses of (2)H from the C-2 position also occurred if choline was converted to betaine by a purified bacterial choline oxidase. To test for an oxygenase, the incorporation of (18)O from (18)O(2) into newly synthesized betaine was compared with that from (18)O-labeled choline, in light and darkness. Incorporation of (18)O from (18)O-choline was readily detectable and varied from about 15 to 50% of the theoretical maximum value; the (18)O losses were attributable to exchange of the intermediate betaine aldehyde with water. In darkness, incorporation of (18)O from (18)O(2) approached that from (18)O-choline, but in the light was severalfold lower, presumably due to isotopic dilution by photosynthetic (16)O(2). These data indicate that the chloroplast choline-oxidizing enzyme is an oxygenase.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Lerma C,Hanson AD,Rhodes D

doi

10.1104/pp.88.3.695

subject

Has Abstract

pub_date

1988-11-01 00:00:00

pages

695-702

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

88

pub_type

杂志文章
  • Characterization and Cloning of Grape Circular RNAs Identified the Cold Resistance-Related Vv-circATS1.

    abstract::Circular RNAs (circRNAs) are widely distributed and play essential roles in a series of developmental processes, although none have been identified or characterized in grapevines (Vitis vinifera). In this study, we characterized the function of grape circRNA and uncovered thousands of putative back-splicing sites by g...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.01331

    authors: Gao Z,Li J,Luo M,Li H,Chen Q,Wang L,Song S,Zhao L,Xu W,Zhang C,Wang S,Ma C

    更新日期:2019-06-01 00:00:00

  • Evidence for Endogenous Cyclic Photophosphorylation in Intact Chloroplasts: CO(2) Fixation with Dihydroxyacetone Phosphate.

    abstract::This study examines the capacity of intact spinach (Spinacia oleracea L.) chloroplasts to fix (14)CO(2) when supplied with Benson-Calvin cycle intermediates in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU). Under these conditions, substantial (14)CO(2) fixation occurred in the light but not in the dar...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.2.321

    authors: Woo KC,Gerbaud A,Furbank RT

    更新日期:1983-06-01 00:00:00

  • Effect of CO2 Concentration on Carbonic Anhydrase and Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Expression in Pea.

    abstract::The effect of external CO2 concentration on the expression of carbonic anhydrase (CA) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) was examined in pea (Pisum sativum cv Little Marvel) leaves. Enzyme activities and their transcript levels were reduced in plants grown at 1000 [mu]L/L CO2 compared with p...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.2.569

    authors: Majeau N,Coleman JR

    更新日期:1996-10-01 00:00:00

  • Nickel: a micronutrient essential for higher plants.

    abstract::Nickel was established as an essential micronutrient for the growth of temperate cereal crops. Grain from barley (Hordeum vulgare L. cv ;Onda'; containing 40 to 80 nanograms of Ni per gram dry weight) grown in solution culture with negligible Ni concentrations (< 30 nanograms of Ni per liter) exhibited greatly reduced...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.3.801

    authors: Brown PH,Welch RM,Cary EE

    更新日期:1987-11-01 00:00:00

  • Uptake and phytotoxicity of the herbicide metsulfuron methyl in corn root tissue in the presence of the safener 1,8-naphthalic anhydride.

    abstract::Growth of Zea mays L. cv Potro roots was inhibited by the herbicide metsulfuron methyl (MSM) at the lowest concentration tested: 5 nanomoles per liter. Pretreatment of corn seeds with commercial 1,8-naphthalic anhydride (NA) at 1% (w/w) partially reversed MSM-induced root growth inhibition. MSM at a concentration of 5...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.2.730

    authors: Milhomme H,Bastide J

    更新日期:1990-06-01 00:00:00

  • Kaempferol 3-O-Galactoside, 7-O-Rhamnoside is the Major Green Fluorescing Compound in the Epidermis of Vicia faba.

    abstract::The vacuoles of lower epidermal strips from Vicia faba exhibit an intrinsic green fluorescence when incubated in alkaline buffers. Using an alkaline-induced absorbance change as a spectrophotometric assay, the major pigment responsible for this fluorescence was isolated and identified as the flavonoid: kaempferol 3-O-...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.2.522

    authors: Vierstra RD,John TR,Poff KL

    更新日期:1982-02-01 00:00:00

  • Energy and Electron Transfer Systems of Chlamydomonas reinhardi: II. Two Cyclic Pathways of Photosynthetic Electron Transfer in the Pale Green Mutant.

    abstract::Light- and oxygen-induced changes of cytochromes f, b(563), and b(559) and ferredoxin-flavoprotein were studied by a double beam spectrophotometer with combinations of inhibitors and lowered temperatures in the whole cells of the pale green mutant of Chlamydomonas reinhardi (ATCC 18302). At room temperature, the stead...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.46.1.163

    authors: Hiyama T,Nishimura M,Chance B

    更新日期:1970-07-01 00:00:00

  • Lipid Peroxide-Derived Short-Chain Carbonyls Mediate Hydrogen Peroxide-Induced and Salt-Induced Programmed Cell Death in Plants.

    abstract::Lipid peroxide-derived toxic carbonyl compounds (oxylipin carbonyls), produced downstream of reactive oxygen species (ROS), were recently revealed to mediate abiotic stress-induced damage of plants. Here, we investigated how oxylipin carbonyls cause cell death. When tobacco (Nicotiana tabacum) Bright Yellow-2 (BY-2) c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.115.256834

    authors: Biswas MS,Mano J

    更新日期:2015-07-01 00:00:00

  • Purification and Characteristics of Sorbitol-6-phosphate Dehydrogenase from Loquat Leaves.

    abstract::To study the role of sorbitol-6-phosphate dehydrogenase in sorbitol synthesis in leaves of Rosaceous plants, properties of the enzyme and its presence in several plants in the family was investigated. The activity of the enzyme, which catalyzes an NADP-dependent oxidation of the substrate to glucose-6-phosphate, was d...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.67.2.221

    authors: Hirai M

    更新日期:1981-02-01 00:00:00

  • Photosynthetic carbon metabolism of isolated corn chloroplasts.

    abstract::Chloroplasts have been isolated from 4- to 6-day-old corn (Zea mays) leaves capable of assimilating 45 micromoles CO(2) per milligram chlorophyll per hour. The effects of various factors such as inorganic phosphate, reducing agents, inhibitors, intermediates of the photosynthetic carbon reduction cycle, organic acids,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.4.607

    authors: O'neal D,Hew CS,Latzko E,Gibbs M

    更新日期:1972-04-01 00:00:00

  • Characterization of cadmium uptake by plant tissue.

    abstract::The uptake of cadmium by excised root tissue of barley (Hordeum vulgare L. cv. Arivat) was investigated with respect to kinetics, concentration, and interactions with various cations. The role of metabolism in Cd absorption was examined using a range of temperatures, anaerobic treatments, and chemical inhibitors. The ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.1.67

    authors: Cutler JM,Rains DW

    更新日期:1974-07-01 00:00:00

  • Effect of polar lipids on ATPase activity of membrane preparations from germinating radish seeds.

    abstract::Membrane preparation (sedimenting between 13,000(g) and 80,000(g)) of germinating radish seeds (Raphanus sativus L.) was active in hydrolyzing ATP and, to a lesser extent, a variety of other phosphorylated compounds. Dicyclohexylcarbodiimide (DCCD) and diethylstilbestrol significantly inhibited the ATPase activity (40...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.2.377

    authors: Cocucci M,Ballarin-Denti A

    更新日期:1981-08-01 00:00:00

  • Role of Ethylene in the Geotropic Response of Bermudagrass (Cynodon dactylon L. Pers.) Stolons.

    abstract::We studied the relationship between ethylene and gravity-induced upward bending of bermudagrass (Cynodon dactylon L. Pers.) stolons. Ethylene production begins within 3 hours of the onset of gravistimulation, and increases thereafter until the 15th hour, after which it declines. There is a close positive relationship ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.4.1251

    authors: Balatti PA,Willemöes JG

    更新日期:1989-12-01 00:00:00

  • Cytochemical localization of catalase activity in glyoxysomes from castor bean endosperm.

    abstract::Glyoxysomes isolated from castor bean (Ricinus communis L. var. zanzibariensis) endosperm have been stained by the cytochemical diaminobenzidine reaction. The reaction product obtained by preincubation with 3,3'-diaminobenzidine and incubation with the reagent and H(2)O(2) is distributed uniformly throughout the matri...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.53.2.276

    authors: Bieglmayer C,Ruis H

    更新日期:1974-02-01 00:00:00

  • Compartmentation of Organic Acids in Corn Roots II. The Cytoplasmic Pool of Malic Acid.

    abstract::The major conclusion drawn was that malate generated in corn roots during a 15-minute period of CO(2) fixation and malate introduced into the tissue during a similar period from the bathing medium share a common extramitochondrial compartment, the cytoplasmic pool. The utilization of these 2 forms of malate is normall...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.4.713

    authors: Lips SH,Beevers H

    更新日期:1966-04-01 00:00:00

  • The arabidopsis cell plate-associated dynamin-like protein, ADL1Ap, is required for multiple stages of plant growth and development.

    abstract::Dynamin and dynamin-like proteins are GTP-binding proteins involved in vesicle trafficking. In soybean, a 68-kD dynamin-like protein called phragmoplastin has been shown to be associated with the cell plate in dividing cells (Gu and Verma, 1996). Five ADL1 genes encoding dynamin-like proteins related to phragmoplastin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.126.1.47

    authors: Kang BH,Busse JS,Dickey C,Rancour DM,Bednarek SY

    更新日期:2001-05-01 00:00:00

  • Climate and Developmental Plasticity: Interannual Variability in Grapevine Leaf Morphology.

    abstract::The shapes of leaves are dynamic, changing over evolutionary time between species, within a single plant producing different shaped leaves at successive nodes, during the development of a single leaf as it allometrically expands, and in response to the environment. Notably, strong correlations between the dissection a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.01825

    authors: Chitwood DH,Rundell SM,Li DY,Woodford QL,Yu TT,Lopez JR,Greenblatt D,Kang J,Londo JP

    更新日期:2016-03-01 00:00:00

  • Three-Dimensional Analysis of Chloroplast Structures Associated with Virus Infection.

    abstract::Chloroplasts are multifunctional organelles whose morphology is affected by environmental stresses. Although the three-dimensional (3D) architecture of thylakoid membranes has been reported previously, a 3D visualization of chloroplast under stress has not been explored. In this work, we used a positive-strand RNA ((+...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00871

    authors: Jin X,Jiang Z,Zhang K,Wang P,Cao X,Yue N,Wang X,Zhang X,Li Y,Li D,Kang BH,Zhang Y

    更新日期:2018-01-01 00:00:00

  • Environmental Parameters Affecting Dark Response of Rice Seedlings (Oryza sativa L.) to Triacontanol.

    abstract::Triacontanol applied to IR-8 rice (Oryza sativa L.) seedlings in nutrient solution caused an increase in dry weight during a 6-hour dark period. This increase was altered by atmospheric CO(2) and O(2) concentrations. The largest growth response occurred from 200 to 350 muliters/liter CO(2) with 5% O(2). The treated se...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.5.851

    authors: Bittenbender HC,Dilley DR,Wert V,Ries SK

    更新日期:1978-05-01 00:00:00

  • Mechanism of photoactivation of electron transport in intact bryopsis chloroplasts.

    abstract::The mechanism of photoactivation of photosystem I electron transport was studied in intact Bryopsis corticulans chloroplasts. The evidence from chemical and photochemical studies suggests that photoactivation is a consequence of a reduction of an electron transport component, presumably ferredoxin-NADP(+) reductase. O...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.5.1413

    authors: Satoh K

    更新日期:1982-11-01 00:00:00

  • In Vitro Prenylation of the Small GTPase Rac13 of Cotton.

    abstract::Previous work (D.P. Delmer, J. Pear, A. Andrawis, D. Stalker [1995] Mol Gen Genet 248: 43-51) has identified a gene in cotton (Gossypium hirsutum), Rac13, that encodes a small, signal-transducing GTPase and shows high expression in the fiber at the time of transition from primary to secondary wall synthesis. Since Rac...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.4.1491

    authors: Trainin T,Shmuel M,Delmer DP

    更新日期:1996-12-01 00:00:00

  • Loss of Microtubules in the Interphase Cells of Onion (Allium cepa L.) Root Tips from the Cell Cortex and Their Appearance in the Cytoplasm after Treatment with Cycloheximide.

    abstract::As part of a project to investigate the mechanism of cortical microtubule (MT) alignment, we examined the effects of cycloheximide (CHM) on cortical MTs in the root tip cells of Allium cepa L. Results show that although a preprophase band of MTs remained in the cell cortex, interphase MTs disappeared from the cortical...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.1.281

    authors: Mineyuki Y,Iida H,Anraku Y

    更新日期:1994-01-01 00:00:00

  • Role of the putative osmosensor Arabidopsis histidine kinase1 in dehydration avoidance and low-water-potential response.

    abstract::The molecular basis of plant osmosensing remains unknown. Arabidopsis (Arabidopsis thaliana) Histidine Kinase1 (AHK1) can complement the osmosensitivity of yeast (Saccharomyces cerevisiae) osmosensor mutants lacking Synthetic Lethal of N-end rule1 and SH3-containing Osmosensor and has been proposed to act as a plant o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.209791

    authors: Kumar MN,Jane WN,Verslues PE

    更新日期:2013-02-01 00:00:00

  • Na-NMR Studies of the Intracellular Sodium Ion Concentration in the Halotolerant Alga Dunaliella salina.

    abstract::The Intracellular Na(+) concentration in the halotolerant alga Dunaliella salina was measured in intact cells by (23)Na-NMR spectroscopy, utilizing the dysprosium tripolyphosphate complex as a sodium shift reagent, and was found to be 88 +/- 28 millimolar. Intracellular sodium ion content and intracellular volume were...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.4.813

    authors: Bental M,Degani H,Avron M

    更新日期:1988-08-01 00:00:00

  • The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro

    abstract::The effects of plant hormones and sucrose (Suc) on potato (Solanum tuberosum L.) tuberization were studied using in vitro cultured single-node cuttings. Tuber-inducing (high Suc) and -noninducing (low Suc or high Suc plus gibberellin [GA]) media were tested. Tuberization frequencies, tuber widths, and stolon lengths w...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.2.575

    authors: Xu X,van Lammeren AA,Vermeer E,Vreugdenhil D

    更新日期:1998-06-01 00:00:00

  • Transcriptome analysis reveals a critical role of CHS7 and CHS8 genes for isoflavonoid synthesis in soybean seeds.

    abstract::We have used cDNA microarray analysis to examine changes in gene expression during embryo development in soybean (Glycine max) and to compare gene expression profiles of two soybean cultivars that differ in seed isoflavonoid content. The analysis identified 5,910 genes that were differentially expressed in both soybea...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.086306

    authors: Dhaubhadel S,Gijzen M,Moy P,Farhangkhoee M

    更新日期:2007-01-01 00:00:00

  • Regulation of alternative oxidase activity in six wild monocotyledonous species. An in vivo study at the whole root level.

    abstract::The activity of the alternative pathway is affected by a number of factors, including the level and reduction state of the alternative oxidase (AOX) protein, and the reduction state of the ubiquinone pool. To investigate the significance of these factors for the rate of alternative respiration in vivo, we studied root...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.126.1.376

    authors: Millenaar FF,Gonzàlez-Meler MA,Fiorani F,Welschen R,Ribas-Carbo M,Siedow JN,Wagner AM,Lambers H

    更新日期:2001-05-01 00:00:00

  • Phosphatidylinositol 4,5-Bisphosphate Phospholipase C and Phosphomonoesterase in Dunaliella salina Membranes.

    abstract::In comparison with other cell organelles, the Dunaliella salina plasma membrane was found to be highly enriched in phospholipase C activity toward exogenous [(3)H]phosphatidylinositol 4,5-bisphosphate (PIP(2)). Based on release of [(3)H]inositol phosphates, the plasma membrane exhibited a PIP(2)-phospholipase C activi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.3.1115

    authors: Einspahr KJ,Peeler TC,Thompson GA

    更新日期:1989-07-01 00:00:00

  • Properties of Solubilized Microsomal Lipase from Germinating Brassica napus.

    abstract::Lipase (triacylglycerol acylhydrolase [EC 3.1.1.3.]) was extracted from the microsomal fraction of cotyledons of dark grown seedlings of Canola (Brassica napus L. cv Westar) by treatment with Triton X-100. The enzyme was partially purified by chromatography on Sephacryl S-300 and DEAE Bio-Gel and was stable when store...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.4.1303

    authors: Weselake RJ,Thomson LW,Tenaschuk D,Mackenzie SL

    更新日期:1989-12-01 00:00:00

  • CHLOROPLAST RIBOSOME ASSOCIATED Supports Translation under Stress and Interacts with the Ribosomal 30S Subunit.

    abstract::Chloroplast ribosomes, which originated from cyanobacteria, comprise a large subunit (50S) and a small subunit (30S) containing ribosomal RNAs (rRNAs) and various ribosomal proteins. Genes for many chloroplast ribosomal proteins, as well as proteins with auxiliary roles in ribosome biogenesis or functioning, reside in...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.00602

    authors: Pulido P,Zagari N,Manavski N,Gawronski P,Matthes A,Scharff LB,Meurer J,Leister D

    更新日期:2018-08-01 00:00:00