Alterations in Chloroplast Thylakoids during an in Vitro Freeze-Thaw Cycle.

Abstract:

:Plastocyanin and chloroplast coupling factor 1 (CF(1)) are released from spinach (Spinacia oleracea L.) thylakoids during a slow freezethaw cycle. CF(1) addition increases the proton uptake of thylakoids previously frozen in sucrose concentrations of 15 mm to 100 mm. Addition of CF(1) and plastocyanin restores the proton uptake of thylakoids frozen in 100 mm sucrose. Plastocyanin and CF(1) release is a manifestation, not the cause, of freeze-thaw damage.Frozen-thawed thylakoids appear to exhibit two levels of response to sucrose as measured by light-dependent proton uptake. Different levels of protection afforded by sucrose may be due, in part, to quantitative differences in CF(1) release. The results suggest at least three freeze-induced lesions in light-dependent proton uptake by thylakoids: plastocyanin release, CF(1) release, and disruption of the semi-permeability of thylakoids.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Garber MP,Steponkus PL

doi

10.1104/pp.57.5.673

subject

Has Abstract

pub_date

1976-05-01 00:00:00

pages

673-80

issue

5

eissn

0032-0889

issn

1532-2548

journal_volume

57

pub_type

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