Functional analysis of the final steps of the 1-deoxy-D-xylulose 5-phosphate (DXP) pathway to isoprenoids in plants using virus-induced gene silencing.

abstract：:Diurnal changes in levels of selected metabolites associated with glycolysis, the C(3) cycle, C(4)-organic acids, and storage carbohydrates were analyzed in active Kalanchoë daigremontiana Crassulacean acid metabolism leaves. Three metabolic transition periods occurred each day. During the first two hours of light, ne...

journal_title：Plant physiology

authors： Kenyon WH,Holaday AS,Black CC

更新日期：1981-11-01 00:00:00

abstract：:C4 photosynthesis evolved more than 60 times independently in different plant lineages. Each time, multiple genes were recruited into C4 metabolism. The corresponding promoters acquired new regulatory features such as high expression, light induction, or cell type-specific expression in mesophyll or bundle sheath cell...

journal_title：Plant physiology

authors： Heimann L,Horst I,Perduns R,Dreesen B,Offermann S,Peterhansel C

更新日期：2013-05-01 00:00:00

abstract：:The rates of CO(2) exchange and (14)CO(2) incorporation in the light and dark and the activities of several photosynthetic, photorespiratory, and respiratory enzymes of soybean (Glycine max [L.] Merr. cv. Wye) reproductive structures were determined at weekly intervals from anthesis to pod maturity. At all stages of p...

journal_title：Plant physiology

authors： Quebedeaux B,Chollet R

更新日期：1975-04-01 00:00:00

abstract：:Biosynthesis of ent-kaurene was investigated in extracts of cell suspension cultures and seedlings of castor bean. Both cell-free extracts contain an inhibitor of kaurene synthetase. The inhibition affects mainly the cyclization of geranylgeranyl pyrophosphate to copalyl pyrophosphate (activity A) and has little or no...

journal_title：Plant physiology

authors： Gafni Y,Shechter I

更新日期：1981-06-01 00:00:00

abstract：:Wheat (Triticum aestivum L. cv Albis) was grown in open-top chambers in the field and fumigated daily with charcoal-filtered air (0.015 microliters per liter O(3)), nonfiltered air (0.03 microliters per liter O(3)), and air enriched with either 0.07 or 0.10 microliters per liter ozone (seasonal 8 hour/day [9 am-5 pm] ...

journal_title：Plant physiology

authors： Lehnherr B,Mächler F,Grandjean A,Fuhrer J

更新日期：1988-12-01 00:00:00

abstract：:Fluxes of mineral nutrients in the xylem are strongly influenced by interactions with the surrounding stem tissues and are probably regulated by them. Toward a mechanistic understanding of these interactions, we applied stable isotope tracers of magnesium, potassium, and calcium continuously to the transpiration strea...

journal_title：Plant physiology

authors： Metzner R,Schneider HU,Breuer U,Thorpe MR,Schurr U,Schroeder WH

更新日期：2010-02-01 00:00:00

abstract：:Although Arabidopsis is well established as the premiere model species in plant biology, rice (Oryza sativa) is moving up fast as the second-best model organism. In addition to the availability of large sets of genetic, molecular, and genomic resources, two features make rice attractive as a model species: it represen...

journal_title：Plant physiology

authors： Rensink WA,Buell CR

更新日期：2004-06-01 00:00:00

abstract：:Water conductance through Abies amabilis seedlings was measured while the roots were exposed to temperatures from 15 to 0.25 degrees C. Before conductance was measured, the seedlings were preconditioned for 3 months at either a high temperature (23 degrees C) or a low temperature (3 degrees C). For both groups of seed...

journal_title：Plant physiology

authors： Teskey RO,Hinckley TM,Grier CC

更新日期：1984-01-01 00:00:00

abstract：:Zinc is an essential mineral for a wide variety of physiological and biochemical processes. To understand zinc transport in cereals, we identified putative zinc transporters in gene databases. Three full-length cDNAs were identified and characterized from rice (Oryza sativa). Two of the cDNAs partially complemented a ...

journal_title：Plant physiology

authors： Ramesh SA,Shin R,Eide DJ,Schachtman DP

更新日期：2003-09-01 00:00:00

abstract：:Aldolase activity of Chara foetida (Braun) could be separated into a minor (peak I) and a major peak (peak II) by ion-exchange chromatography on DEAE-cellulose. Affinity chromatography on P-cellulose resulted in highly purified aldolase preparations with specific activities of 3.2 and 4.8 units per milligram protein a...

journal_title：Plant physiology

authors： Jacobshagen S,Schnarrenberger C

更新日期：1988-05-01 00:00:00

abstract：:A rapid and simple, large-scale method for the purification of DNA-dependent RNA polymerase III (EC 2.7.7.6) from wheat germ is presented. The method involves enzyme extraction at low ionic strength, polyethyleneimine fractionation, (NH(4))(2)SO(4) precipitation, and chromatography on DEAE-Sepharose CL-6B, DEAE-cellul...

journal_title：Plant physiology

authors： Jendrisak J

更新日期：1981-03-01 00:00:00

abstract：:Excised juvenile leaves of Microgramma vacciniifolia (Polypodiaceae) develop sporophytic regenerants when grown on mineral agar with sucrose. The ratio of sporophytes to gametophytes produced from the leaf tissue increases with higher percentages of sucrose such that at 4% sucrose, the induction of aposporous gametoph...

journal_title：Plant physiology

authors： Hirsch AM

更新日期：1975-09-01 00:00:00

abstract：:Crown galls induced by infection of tobacco plants with Agrobacterium tumefaciens strain C58-Cl(pTiB6S3) were excised and cultured in vitro. After about one year of culture on medium-lacking phytohormones, two noncloned lines spontaneously formed shoots. Leaf explants from shoots of tumor-line T5 were capable of growi...

journal_title：Plant physiology

authors： Owens LD

更新日期：1982-01-01 00:00:00

abstract：:The vascular cambium produces secondary xylem and phloem in plants and is responsible for wood formation in forest trees. In this study we used a microscale mass-spectrometry technique coupled with cryosectioning to visualize the radial concentration gradient of endogenous indole-3-acetic acid (IAA) across the cambial...

journal_title：Plant physiology

authors： Uggla C,Mellerowicz EJ,Sundberg B

更新日期：1998-05-01 00:00:00

abstract：:Plant growth and development are strongly dependent on sink-source interactions. In the majority of plants, sucrose (Suc) is the dominant form in which photo-assimilate is transported from source to sinks. Although the effects of Suc on photosynthetic metabolism have been intensively studied, the effect of Suc supply ...

journal_title：Plant physiology

authors： Roessner-Tunali U,Urbanczyk-Wochniak E,Czechowski T,Kolbe A,Willmitzer L,Fernie AR

更新日期：2003-10-01 00:00:00

abstract：:Protein turnover is a key component in cellular homeostasis; however, there is little quantitative information on degradation kinetics for individual plant proteins. We have used (15)N labeling of barley (Hordeum vulgare) plants and gas chromatography-mass spectrometry analysis of free amino acids and liquid chromatog...

journal_title：Plant physiology

authors： Nelson CJ,Alexova R,Jacoby RP,Millar AH

更新日期：2014-09-01 00:00:00

abstract：:We identified a recessive, brassinolide-insensitive mutant caused by a deletion allele (bri1-201) of the brassinosteroid (BR) receptor BRI1. The bri1-201 mutant displayed altered expression levels of genes differentially regulated by gibberellin (GA). RNA-blot analysis revealed that BR and GA antagonistically regulate...

journal_title：Plant physiology

authors： Bouquin T,Meier C,Foster R,Nielsen ME,Mundy J

更新日期：2001-10-01 00:00:00

abstract：:Endo-polygalacturonase (PG) was purified from a commercial preparation of Aspergillus niger pectinase by means of carboxymethylcellulose chromatography, preparative isoelectric focusing, and gel permeation through Sephadex G-50. The enzyme was electrophoretically homogeneous and consisted of a single polypeptide chain...

journal_title：Plant physiology

authors： Cervone F,De Lorenzo G,Degrà L,Salvi G

更新日期：1987-11-01 00:00:00

abstract：:We compared the subcellular distribution of native and artificial reticuloplasmins in endosperm, callus, and leaf tissues of transgenic rice (Oryza sativa) to determine the distribution of these proteins among endoplasmic reticulum (ER) and post-ER compartments. The native reticuloplasmin was calreticulin. The artific...

journal_title：Plant physiology

authors： Torres E,Gonzalez-Melendi P,Stöger E,Shaw P,Twyman RM,Nicholson L,Vaquero C,Fischer R,Christou P,Perrin Y

更新日期：2001-11-01 00:00:00

abstract：:The relationship between the state of oligomerization and activity of purified maize leaf phosphoenolpyruvate carboxylase using size exclusion high performance liquid chromatography was examined. Maximum activities of 35 to 38 micromoles per minute per milligram protein were found when 100% of the enzyme was in its te...

journal_title：Plant physiology

authors： Walker GH,Ku MS,Edwards GE

更新日期：1986-04-01 00:00:00

abstract：:Hydrogen peroxide (H2O2) is a stable component of reactive oxygen species, and its production in plants represents the successful recognition of pathogen infection and pathogen-associated molecular patterns (PAMPs). This production of H2O2 is typically apoplastic but is subsequently associated with intracellular immun...

journal_title：Plant physiology

authors： Tian S,Wang X,Li P,Wang H,Ji H,Xie J,Qiu Q,Shen D,Dong H

更新日期：2016-07-01 00:00:00

abstract：:Virus-induced gene silencing (VIGS) is used extensively for gene function studies in plants. VIGS is inexpensive and rapid compared with silencing conducted through stable transformation, but many virus-silencing vectors, especially in grasses, induce only transient silencing phenotypes. A major reason for transient p...

journal_title：Plant physiology

authors： Ding XS,Mannas SW,Bishop BA,Rao X,Lecoultre M,Kwon S,Nelson RS

更新日期：2018-01-01 00:00:00

abstract：:One of the most abundant proteins in maize (Zea mays L.) embryos is the molecular weight 63,000 globulin encoded by the Glb1 gene. To obtain DNA clones corresponding to Glb1, a cDNA library corresponding to RNA from developing maize embryos was constructed in a lambda expression vector and screened with antibodies spe...

journal_title：Plant physiology

authors： Belanger FC,Kriz AL

更新日期：1989-10-01 00:00:00

abstract：:Glycine decarboxylase is a mitochondrial enzyme complex, which is the site of photorespiratory CO2 and NH3 release. Although the proteins that constitute the complex are located within the mitochondria, because of their intimate association with photosynthesis their expression is controlled by light. Comparisons of th...

journal_title：Plant physiology

authors： Srinivasan R,Oliver DJ

更新日期：1995-09-01 00:00:00

abstract：:The isolation of chloroplast and nuclear DNA from dark- and light-grown, control- and 3-amino-1,2,4-triazole-treated wheat seedlings, Triticum vulgare, is described. Contrary to a previous report, we found that chloroplast and nuclear DNA had similar buoyant densities (1.702 grams per cubic centimeter) and that they c...

journal_title：Plant physiology

authors： Bartels PG,Hyde A

更新日期：1970-12-01 00:00:00

abstract：:Amitrole (3-amino-s-triazole), dichlormate (3,4-dichlorobenzyl methylcarbamate), and pyriclor (2,3,5-trichloro-4-pyridinol) inhibited normal carotenogenesis in etiolated wheat (Triticum aestivum L. var. Coker 65-20) seedlings. Carotenoid precursors accumulated in treated plants. In dichlormate-treated plants, zeta-car...

journal_title：Plant physiology

authors： Burns ER,Buchanan GA,Carter MC

更新日期：1971-01-01 00:00:00

abstract：:Posttranslational modifications of proteins are key effectors of enzyme activity, protein interactions, targeting, and turnover rate, but despite their importance, they are still poorly understood in plants. Although numerous reports have revealed the regulatory role of protein phosphorylation in photosynthesis, vario...

journal_title：Plant physiology

authors： Lehtimäki N,Koskela MM,Mulo P

更新日期：2015-07-01 00:00:00

abstract：:Root respiration associated with symbiotic fixation in soybean (Glycine max [L.] Merr.) was estimated by four methods.Averaged over the life of the plant, the root respires 5.8 milligrams C per milligram N accumulated from fixation. When nitrogenase (C(2)H(2)) activity and root respiration were decreased by treating r...

journal_title：Plant physiology

authors： Patterson TG,Larue TA

更新日期：1983-07-01 00:00:00

abstract：:Boron is an essential micronutrient for plant growth and the boron content of plants differs greatly, but the mechanism(s) of its uptake into cells is not known. Boron is present in the soil solution as boric acid and it is in this form that it enters the roots. We determined the boron permeability coefficient of puri...

journal_title：Plant physiology

authors： Dordas C,Chrispeels MJ,Brown PH

更新日期：2000-11-01 00:00:00

abstract：:The control of night-break timing was studied in dark-grown seedlings of Pharbitis nil (Choisy cv. Violet) following a single continuous or skeleton photoperiod. There was a rhythmic response to a red (R) interruption of an inductive dark period, and the phasing of the rhythm was influenced by the preceding light trea...

journal_title：Plant physiology

authors： Lumsden P,Thomas B,Vince-Prue D

更新日期：1982-07-01 00:00:00